Article
Multidisciplinary Sciences
Seunghyi Kook, Ping Wang, Shufang Meng, Christopher S. Jetter, Jennifer M. S. Sucre, John T. Benjamin, Jason J. Gokey, Hayley A. Hanby, Alexa Jaume, Laura Goetzl, Michael S. Marks, Susan H. Guttentag
Summary: Lamellar bodies (LBs) are lysosome-related organelles (LROs) in AT2 cells that play a crucial role in cell homeostasis. Disruption of trafficking pathways mediated by AP-3 can lead to pulmonary fibrosis through affecting the transport of ATP8A1 to LBs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Ophthalmology
M. Helena Cardoso, Michael J. Hall, Thomas Burgoyne, Pedro Fale, Tina Storm, Cristina Escrevente, Pedro Antas, Miguel C. Seabra, Clare E. Futter
Summary: This study aimed to model the effects of chloroquine on the retinal pigment epithelium in vivo and investigate the impact of mild chloroquine treatment on lysosome function and turnover. The research found that low-dose chloroquine treatment inhibits lysosome reformation, leading to a gradual depletion of lysosomes' ability to interact with cargo-carrying vacuoles and degrade their content. The accumulation of pro-cathepsin D and LAMP1 reflects the inhibition of normal turnover of lysosomal constituents and possibly lysosomes themselves. Understanding the mechanisms underlying lysosome reformation may contribute to the development of new treatments for chloroquine-induced retinopathy.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Cell Biology
Peike Wang, Shuailin Du, Chuanhui Guo, Zhangli Ni, Ziying Huang, Na Deng, Haili Bao, Wenbo Deng, Jinhua Lu, Shuangbo Kong, Hua Zhang, Haibin Wang
Summary: During pregnancy, the blastocyst affects the differentiation of uterine epithelial cells by activating lysosomes, thereby promoting embryo implantation. The blastocyst-derived IGF2 activates STAT3 in epithelial cells, inducing the expression of lysosomal enzymes and further promoting uterine epithelial cell differentiation. Proteomic data shows that the blastocyst-induced lysosomes degrade CLDN1 and MUC1, molecules that are known to inhibit successful implantation.
Article
Critical Care Medicine
Samir Rustam, Yang Hu, Seyed Babak Mahjour, Andre F. Rendeiro, Hiranmayi Ravichandran, Andreacarola Urso, Frank D'Ovidio, Fernando J. Martinez, Nasser K. Altorki, Bradley Richmond, Vasiliy Polosukhin, Jonathan A. Kropski, Timothy S. Blackwell, Scott H. Randell, Olivier Elemento, Renat Shaykhiev
Summary: The remodeling and loss of distal conducting airways, including preterminal and terminal bronchioles, contribute to progressive airflow limitation in COPD. However, the cellular mechanisms underlying these structural changes are still unknown.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Article
Multidisciplinary Sciences
Sarah Mustaly-Kalimi, Wacey Gallegos, Robert A. Marr, Alice Gilman-Sachs, Daniel A. Peterson, Israel Sekler, Grace E. Stutzmann
Summary: Impairments in Ca2+ signaling disrupt lysosomal acidification and contribute to pathological protein aggregation in neurodegenerative disorders like AD. Targeting the RyR-Ca2+ pathway could be a potential therapeutic intervention.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Oncology
Yi Wu, Peng Huang, Xian-Ping Dong
Summary: The importance of autophagy and lysosomal calcium channels in maintaining cellular homeostasis and their roles in cancer development are discussed in this review. The review highlights the significance of intracellular calcium signaling and lysosomal calcium stores in regulating various cellular processes and their implications in human diseases, particularly cancer.
Article
Cell Biology
Dongun Lee, Jeong Hee Hong
Summary: Lysosomal chloride levels are crucial for lysosomal function and cellular processes such as migration and apoptosis. Reduced chloride levels induce lysosomal calcium release and repositioning, leading to dysregulation of cancer progression.
Article
Cell Biology
Anna Acheva, Tytti Karki, Niccole Schaible, Ramaswamy Krishnan, Sari Tojkander
Summary: Obesity is a major risk factor for the development of breast cancer in postmenopausal women, with the adipokine leptin strongly linked to tumor cell proliferation and metastasis. Leptin activates leptin receptors and mechanosensitive Ca2+ channels to regulate actomyosin dynamics, thus playing a role in the development of breast carcinomas.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Chenyan Li, Yangyang Hou, Minwei He, Liping Lv, Yulong Zhang, Sujing Sun, Yan Zhao, Xingzhao Liu, Ping Ma, Xiaohui Wang, Qianqian Zhou, Linsheng Zhan
Summary: This study evaluated the effect of Lap on DCs and found that Lap dramatically improved the homing ability of DCs to lymphoid tissues and enhanced antigen-specific CD8+ T-cell activation. Additionally, a Lap@C-C adjuvant was constructed for DC vaccines, which initiated a robust cytotoxic T-cell immune response against hepatitis B infection. These findings highlight the potential value of Lap as a DC vaccine adjuvant and provide a basis for effective DC vaccine development.
Article
Biochemistry & Molecular Biology
Kei Takahashi, Shinsuke Nakamura, Masamitsu Shimazawa, Hideaki Hara
Summary: This study characterized the retinal phenotype in mature PGRN knockout mice, showing reduced a-wave amplitude and outer nuclear thickness in Grn(-/-) mice compared to wild-type mice. Increased accumulation of retinal microglial cells on the RPE apical layer and changes in lysosomal protein expression were observed in Grn(-/-) mice, indicating a potential modulation of microglial dynamics in PGRN deficiency.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jessica B. Blackburn, Jacob A. Schaff, Sergey Gutor, Rui-Hong Du, David Nichols, Taylor Sherrill, Austin J. Gutierrez, Matthew K. Xin, Nancy Wickersham, Yong Zhang, Michael J. Holtzman, Lorraine B. Ware, Nicholas E. Banovich, Jonathan A. Kropski, Timothy S. Blackwell, Bradley W. Richmond
Summary: Loss of secretory IgA (SIgA) in chronic obstructive pulmonary disease (COPD) small airways is caused by reduced expression of polymeric immunoglobulin receptor (pIgR) and degradation of SIgA by neutrophil elastase and nontypeable Haemophilus influenzae-secreted proteases.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Cell Biology
Mateus Milani, Philippe Pihan, Claudio Hetz
Summary: Calcium is a crucial messenger in regulating cellular processes and interorganelle communication. Lysosomal function is dependent on calcium, and ion channels on the lysosomal membrane regulate lysosomal properties such as pH. Lysosome-dependent cell death (LDCD) is a specific type of cell death involving lysosomes that plays important roles in tissue homeostasis, development, and pathology. This article focuses on recent advances in understanding the role of calcium signaling in LDCD.
Review
Cell Biology
Johannes Nass, Julian Terglane, Volker Gerke
Summary: Vascular endothelial cells play a crucial role in regulating vascular functions and interactions with blood cells to ensure smooth blood flow and specific responses to vascular injury and inflammation. These cells have the ability to rapidly change their surface properties to control blood flow effectively.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Juliette L. Simonin, Alexandre Luscher, Davide Losa, Mehdi Badaoui, Christian van Delden, Thilo Kohler, Marc Chanson
Summary: Defective hydration of airway surface mucosa is associated with recurrent lung infection in cystic fibrosis. The presence of a healthy airway surface liquid can prevent infection.
Article
Medicine, Research & Experimental
Kei Takahashi, Shinsuke Nakamura, Masamitsu Shimazawa, Hideaki Hara
Summary: The study found that deficiency of progranulin disrupts lysosomal activity and physiological functions in retinal pigment epithelium (RPE) cells. Additionally, loss of progranulin decreases cell viability and disrupts cell-cell junctions.
