Article
Food Science & Technology
Xue Qin, Yitian Wang, Hongxuan Li, Xinyan Yang, Zhenghui Wang, Qing Chen, Chaoxin Man, Yujun Jiang
Summary: A visual detection strategy based on DNAzyme and asymmetry recombinase polymerase amplification (aRPA) was developed for monitoring Cronobacter sakazakii in powdered infant formula (PIF). The method showed low limits of detection and good stability in on-site simulation evaluation.
Article
Biotechnology & Applied Microbiology
Zhenbo Xu, Ziqi Liu, Thanapop Soteyome, Jingjing Hua, Liang Zhang, Lei Yuan, Yanrui Ye, Zhao Cai, Liang Yang, Ling Chen, Janette M. Harro, Birthe Veno Kjellerup, Junyan Liu, Yanyan Li
Summary: The study investigated the transcriptional regulation of the Cronobacter sakazakii pmrA mutant during biofilm formation, revealing important genes involved in different stages of biofilm formation.
Article
Food Science & Technology
Dongdong Zhu, Yufei Fan, Xiaoyi Wang, Ping Li, Yaping Huang, Jingbo Jiao, Chumin Zhao, Yue Li, Shuo Wang, Xinjun Du
Summary: The GroEL protein of C. sakazakii plays a crucial role in the bacterium's adaptation to dry and high-temperature environments, and can induce a strong immune response. It is found both inside and outside the bacterial cells and has virulent effects, including adhesion and invasion of host cells.
Article
Food Science & Technology
Ondrej Holy, Julio Parra-Flores, Sarah Lepuschitz, Maria Paula Alarcon-Lavin, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Jetsi Mancilla-Rojano, Werner Ruppitsch, Stephen Forsythe
Summary: This study investigated the putative virulence factors of six Cronobacter sakazakii strains isolated from powdered milk, identifying different sequence types and resistance genes. Some strains demonstrated high adherence and invasion abilities, while the cpa gene was not detected. Various virulence and resistance genes, as well as plasmids, were identified through whole-genome sequencing. Additionally, CRISPR matrices were observed in all strains, enhancing decision-making for health authorities to protect public health.
Article
Immunology
Katie Phair, Sonia Gonsalves Pereira, Carmel Kealey, Seamus Fanning, Damien B. Brady
Summary: Cronobacter species enhance their virulence through various mechanisms during survival in the environment, infection of hosts, and persistence inside hosts. Cronobacter sakazakii is associated with fatal infections in populations such as neonates and infants.
MICROBIAL PATHOGENESIS
(2022)
Article
Food Science & Technology
Yan Ma, Yingying Zhang, Zhongguo Shan, Xin Wang, Xiaodong Xia
Summary: The study investigated the impact of PhoP/PhoQ two component system on biofilm formation in Cronobacter sakazakii BAA-894. Results showed that the system played crucial roles in regulating gene expression, cell viability, and extracellular polymeric substance content, suggesting it as a potential target for controlling biofilms.
Article
Food Science & Technology
Danliangmin Song, Qunchao Su, Ai Jia, Shiqian Fu, Xiaoming Ma, Tiantian Li, Chaoxin Man, Xinyan Yang, Yujun Jiang
Summary: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid identification technology for microorganisms. A new, low-cost liquid spotting pretreatment method was developed and optimized using response surface methodology. The method accurately detected and quantified Cronobacter sakazakii, with a 100% identification accuracy for 70 strains of isolates.
Article
Food Science & Technology
Lingli Luo, Lanhua Yi, Jiaxin Chen, Bianfang Liu, Xin Lu
Summary: Bacteriocin BM1157 inhibits Gram-negative bacteria by disrupting their metabolic processes, inhibiting biofilm formation, and ultimately causing cell death.
Article
Biotechnology & Applied Microbiology
Ping Lu, Juan Xue, Xi Chen, Xuemeng Ji
Summary: Our study reveals the critical role of the dnaK gene in virulence, adhesion, invasion, and acid resistance of Cronobacter sakazakii. Proteomic analysis shows that dnaK knockout leads to upregulation of certain proteins and deamidation of others. The findings suggest that DnaK-mediated protein deamidation may be a novel mechanism for virulence and stress adaptation in Cronobacter sakazakii.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Food Science & Technology
Yufei Fan, Ping Li, Dongdong Zhu, Chumin Zhao, Jingbo Jiao, Xuemeng Ji, Xinjun Du
Summary: This study aims to elucidate the precise roles of the ESA_00986 gene in C. sakazakii by generating gene knockout mutants and complementary strains. The results showed that the ESA_00986 gene plays a positive role in biofilm formation, cell adhesion and invasion, and virulence, demonstrating it as a novel virulence factor and advancing our understanding of the pathogenic mechanism of C. sakazakii.
Article
Food Science & Technology
Yi Sun, Jiahui Li, Yanpeng Yang, Gaoji Yang, Yiqi Shi, Shuo Wang, Muxue Wang, Xiaodong Xia
Summary: This study investigates the role of ptsH in stress response and virulence in C. sakazakii. The Delta ptsH mutant displays slower growth rate, decreased resistance to various stresses, and altered gene expression patterns compared to the wild-type. Furthermore, RNA-seq analysis reveals the upregulation of sulfur metabolism pathway and downregulation of bacterial secretion system pathway in the Delta ptsH mutant.
Article
Food Science & Technology
Shuyan Wu, Pornchanok Subharat, Gale Brightwell
Summary: The study found that the combination of blue light LED and external hydrogen peroxide (H2O2) can significantly reduce the number of Cronobacter sakazakii cells, especially for stationary phase and biofilm cells.
Article
Chemistry, Analytical
Hye Ri Kim, Myunghee Kim, Byoung Chan Kim
Summary: In this study, C. sakazakii-specific aptamers were isolated using a centrifugation-based partitioning method, which showed high affinity and specificity for the pathogen. Among the two aptamers isolated, SC25 displayed efficient detection of C. sakazakii in powdered infant formula with less cross-reactivity, potentially reducing overall testing time compared to traditional methods.
Article
Chemistry, Analytical
Yao Zhang, Xingrui Liao, Gege Yu, Juan Wei, Peng Wang, Yueqi Wang, Yinnan Jing, Jiamin Wang, Pengyu Chen, Jianlong Wang, Hong Wang, Yanru Wang
Summary: The development of nanobodies devoid of the Fc fraction has been described for the first time, which enables the real-time monitoring of foodborne pathogens. The phage-mediated sandwich chemiluminescent enzyme immunoassay (CISA) showed high sensitivity for detecting Cronobacter sakazakii.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Yifang Cao, Li Li, Yan Zhang, Fengsong Liu, Xinglong Xiao, Xiaofeng Li, Yigang Yu
Summary: The study shows that sdiA affects biofilm formation in C. sakazakii by regulating gene expression related to bacterial motility and biofilm characteristics. Biofilm formation increased under low osmotic stress conditions and decreased under high osmotic stress conditions.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biotechnology & Applied Microbiology
Julio Parra-Flores, Eduard Maury-Sintjago, Alejandra Rodriguez-Fernandez, Sergio Acuna, Fabiola Cerda, Juan Aguirre, Ondrej Holy
JOURNAL OF FOOD PROTECTION
(2020)
Article
Biotechnology & Applied Microbiology
P. V. Costa, R. M. de Siqueira, A. C. Rosa Guimaraes, L. Vasconcellos, V. Midlej, G. M. Silva da Conceicao, S. J. Forsythe, M. L. Lima Brandao
Summary: This study evaluated the cytotoxic activity of Cronobacter strains isolated from foods and clinical samples in Brazil, and identified some strains capable of producing cytotoxins.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Food Science & Technology
Na Ling, Yujun Jiang, Haiyan Zeng, Yu Ding, Stephen Forsythe
Summary: This review discusses the progress made in China regarding the research on Cronobacter, including large-scale surveys and the adoption of DNA sequence-based methods for identification and genotyping of the organism. These methods have significantly advanced understanding of the taxonomy, ecology, and virulence of Cronobacter, leading to improved source tracking in infant formula production facilities and epidemiological investigations.
JOURNAL OF FOOD SCIENCE
(2021)
Article
Food Science & Technology
Ondrej Holy, Julio Parra-Flores, Sarah Lepuschitz, Maria Paula Alarcon-Lavin, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Jetsi Mancilla-Rojano, Werner Ruppitsch, Stephen Forsythe
Summary: This study investigated the putative virulence factors of six Cronobacter sakazakii strains isolated from powdered milk, identifying different sequence types and resistance genes. Some strains demonstrated high adherence and invasion abilities, while the cpa gene was not detected. Various virulence and resistance genes, as well as plasmids, were identified through whole-genome sequencing. Additionally, CRISPR matrices were observed in all strains, enhancing decision-making for health authorities to protect public health.
Review
Agriculture, Dairy & Animal Science
Lihan Wang, Stephen J. Forsythe, Xinyan Yang, Shiqian Fu, Chaoxin Man, Yujun Jiang
Summary: Cronobacter genus includes food-borne pathogens that can cause severe infections in infants, with a high mortality rate. Advanced detection technology and analysis of high-frequency species from different sources can aid in targeted control of Cronobacter. The bacteria's resistance to environmental stresses and the development of new control techniques highlight the importance of strict monitoring and prevention throughout food processing and storage.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Microbiology
Julio Parra-Flores, Ondrej Holy, Francisca Riffo, Sarah Lepuschitz, Eduard Maury-Sintjago, Alejandra Rodriguez-Fernandez, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Jetsi Mancilla-Rojano, Miriam Troncoso, Guillermo Figueroa, Werner Ruppitsch, Stephen Forsythe
Summary: This study used whole genome sequencing and laboratory studies to characterize Cronobacter strains from contaminated products, revealing multiple antibiotic resistance and virulence genes, supporting the decision to recall contaminated powdered and dairy formulas from the Chilean market in 2017.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Julio Parra-Flores, Ondrej Holy, Francisca Riffo, Sarah Lepuschitz, Werner Ruppitsch, Stephen Forsythe
Summary: Cronobacter sakazakii is a pathogen that causes severe diseases in infants under 12 months, such as meningitis and necrotizing enterocolitis. Seven C. sakazakii genome sequences isolated from PIF and dairy products in Chile in 2017 are presented.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Microbiology
Julio Parra-Flores, Ondrej Holy, Fernanda Bustamante, Sarah Lepuschitz, Ariane Pietzka, Alejandra Contreras-Fernandez, Claudia Castillo, Catalina Ovalle, Maria Paula Alarcon-Lavin, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Jetsi Mancilla-Rojano, Miriam Troncoso, Guillermo Figueroa, Werner Ruppitsch
Summary: This study investigated the virulence factors and antibiotic resistance in Listeria monocytogenes strains isolated from ready-to-eat foods. The results showed that these strains carried virulence and antibiotic resistance traits that pose a potential public health risk for consumers.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Nutrition & Dietetics
Eduard Maury-Sintjago, Alejandra Rodriguez-Fernandez, Julio Parra-Flores, Marcela Ruiz-De la Fuente
Summary: The aim of the study was to determine the association between menstrual cycle phase and resting metabolic rate (RMR) as well as dietary intake. The results showed that lean women had increased RMR, caloric intake, and lipid intake during the luteal phase, while women with obesity only had increased carbohydrate intake.
Article
Environmental Sciences
Alejandra Rodriguez-Fernandez, Natalia Ramos-Castillo, Marcela Ruiz-De la Fuente, Julio Parra-Flores, Eduard Maury-Sintjago
Summary: Gestational exposure to PM2.5 and PM10 particulate matter is associated with prematurity and low birth weight in Chilean newborns. PM10 affects the entire pregnancy, while PM2.5 is primarily associated with low birth weight in the second trimester.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Nutrition & Dietetics
Alejandra Rodriguez-Fernandez, Ximena Sanhueza-Riquelme, Gloria Carcamo-Vargas, Julio Parra-Flores, Ana Lizette Rojas-Rodriguez, Marcela Ruiz-De la Fuente, Eduard Maury-Sintjago
Summary: There is limited knowledge regarding the factors influencing non-exclusive breastfeeding (NEBF) practices in immigrant populations, particularly in Latin America. This study aimed to determine the association between maternal factors and the prevalence of NEBF in Haitian immigrant women in southern Chile. The findings showed that Haitian immigrant women without legal residency, recently arrived, with low educational attainment, and limited knowledge of breastfeeding are at higher risk of not providing exclusive breastfeeding. However, employment, access to basic services, and Spanish proficiency were found to be protective factors associated with higher rates of exclusive breastfeeding.
Article
Pediatrics
Alejandra Rodriguez-Fernandez, Marcela Ruiz-De la Fuente, Ximena Sanhueza-Riquelme, Julio Parra-Flores, Maria Dolores Marrodan, Eduard Maury-Sintjago
Summary: The study found that maternal factors, such as educational attainment and age, are associated with preterm and low-birthweight births in Chile. Mothers with less than 12 years of education and mothers aged 35 or older were found to be at a higher risk for preterm and low-birthweight newborns.
Article
Food Science & Technology
Julio Parra-Flores, Adriana Cabal-Rosel, Beatriz Daza-Prieto, Pamela Chavarria, Eduard Maury-Sintjago, Alejandra Rodriguez-Fernandez, Sergio Acuna, Werner Ruppitsch
Summary: This study genetically characterized Enterobacteriaceae and Enterococcus strains isolated from powdered infant formulas (PIF). The strains exhibited resistance to multiple antibiotics and carried various resistance and virulence genes. These multidrug-resistant strains pose a health risk for infants consuming PIF.
Article
Infectious Diseases
Ondrej Holy, Julio Parra-Flores, Jaroslav Bzdil, Adriana Cabal-Rosel, Beatriz Daza-Prieto, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Ricardo Rodriguez-Martinez, Sergio Acuna, Stephen Forsythe, Werner Ruppitsch
Summary: The objective of this study was to screen for antibiotic resistance, fitness and virulence genes in Cronobacter sakazakii strains isolated from food and powdered-milk-producing environments using whole-genome sequencing. Antibiotic-resistance genes and virulence genes were detected using various tools, and susceptibility testing was performed. The strains showed resistance to cephalotin and ampicillin, and multiple ARGs and VGs were identified. These findings suggest a potential risk of infection in susceptible populations.
Article
Microbiology
Fernanda Bustamante, Eduard Maury-Sintjago, Fabiola Cerda Leal, Sergio Acuna, Juan Aguirre, Miriam Troncoso, Guillermo Figueroa, Julio Parra-Flores
Article
Biotechnology & Applied Microbiology
Gemechu G. Abdi, Yetenayet B. Tola, Chala G. Kuyu
Summary: This study evaluated the quality of honey in the supply chain in southwest Ethiopia and found significant differences in physicochemical and microbial quality among different actors. The study also developed a predictive model to detect adulteration, which showed good linearity and predictive capacity.
JOURNAL OF FOOD PROTECTION
(2024)
Article
Biotechnology & Applied Microbiology
Kathleen A. Glass, Jie Yin Lim, Quinn L. Singer
Summary: Commercial cheese brines can be a reservoir for salt-tolerant pathogens. This study found that the addition of hydrogen peroxide can effectively reduce the population of L. monocytogenes in cheese brines, especially at higher temperatures and salt concentrations. The presence of indigenous microorganisms may neutralize the effect of hydrogen peroxide.
JOURNAL OF FOOD PROTECTION
(2024)