Article
Food Science & Technology
Babak Pakbin, Wolfram Manuel Bruck, Samaneh Allahyari, John W. A. Rossen, Razzagh Mahmoudi
Summary: This study investigated the prevalence, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of C. sakazakii strains isolated from powdered infant formula milk (PIFM) samples collected in Tehran, Iran. The results showed a high prevalence rate of C. sakazakii in PIFM samples, with high resistance to multiple antibiotics and a diverse genetic profile. The study highlights the need for novel microbial surveillance systems to control the contamination of this foodborne pathogen in infant foods.
Article
Food Science & Technology
Ondrej Holy, Julio Parra-Flores, Sarah Lepuschitz, Maria Paula Alarcon-Lavin, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Jetsi Mancilla-Rojano, Werner Ruppitsch, Stephen Forsythe
Summary: This study investigated the putative virulence factors of six Cronobacter sakazakii strains isolated from powdered milk, identifying different sequence types and resistance genes. Some strains demonstrated high adherence and invasion abilities, while the cpa gene was not detected. Various virulence and resistance genes, as well as plasmids, were identified through whole-genome sequencing. Additionally, CRISPR matrices were observed in all strains, enhancing decision-making for health authorities to protect public health.
Article
Food Science & Technology
Danliangmin Song, Xuehe Qi, Yan Huang, Ai Jia, Yaqi Liang, Chaoxin Man, Xinyan Yang, Yujun Jiang
Summary: Cronobacter species are opportunistic foodborne pathogens that pose a threat to infant health. This study investigates the relationship between antibiotic tolerance and virulence in Cronobacter, and identifies proteins implicated in virulence and antibiotic resistance through proteomics analysis. The findings suggest that antibiotic tolerance in Cronobacter is associated with increased production of enterotoxin and hemolysin, and certain strains exhibit decreased utilization of sialic acid. The study also reveals different proteomes and virulence-related proteins in strains with different antibiotic tolerance.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Microbiology
Julio Parra-Flores, Ondrej Holy, Francisca Riffo, Sarah Lepuschitz, Eduard Maury-Sintjago, Alejandra Rodriguez-Fernandez, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Jetsi Mancilla-Rojano, Miriam Troncoso, Guillermo Figueroa, Werner Ruppitsch, Stephen Forsythe
Summary: This study used whole genome sequencing and laboratory studies to characterize Cronobacter strains from contaminated products, revealing multiple antibiotic resistance and virulence genes, supporting the decision to recall contaminated powdered and dairy formulas from the Chilean market in 2017.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Julio Parra-Flores, Adriana Cabal-Rosel, Beatriz Daza-Prieto, Pamela Chavarria, Eduard Maury-Sintjago, Alejandra Rodriguez-Fernandez, Sergio Acuna, Werner Ruppitsch
Summary: This study genetically characterized Enterobacteriaceae and Enterococcus strains isolated from powdered infant formulas (PIF). The strains exhibited resistance to multiple antibiotics and carried various resistance and virulence genes. These multidrug-resistant strains pose a health risk for infants consuming PIF.
Article
Food Science & Technology
Hongxuan Li, Shiqian Fu, Danliangmin Song, Xue Qin, Wei Zhang, Chaoxin Man, Xinyan Yang, Yujun Jiang
Summary: In this study, 35 Cronobacter strains isolated from powdered infant formula and its processing environment were identified and typed using 16S rRNA sequencing and multilocus sequence typing (MLST) technology. A total of 35 different sequence types were obtained, including three new ones that were isolated for the first time. The antibiotic resistance analysis revealed that all isolates were resistant to erythromycin but sensitive to ciprofloxacin. Moreover, 68.57% of the strains showed multi-drug resistance, with the most drug-resistant Cronobacter strain exhibiting resistance to 13 different drugs. Transcriptomics analysis identified 77 differentially expressed genes related to drug resistance, and the study further revealed that Cronobacter strains can activate multidrug efflux systems to enhance drug resistance. Understanding the drug resistance and mechanisms of Cronobacter is of great public health significance for the rational selection and development of antibacterial drugs, as well as the control and treatment of infections caused by this pathogenic microorganism.
Article
Agriculture, Dairy & Animal Science
Ju Liu, Guoyang Xie, Qin Xiong, Dan Mu, Hengyi Xu
Summary: A simple and sensitive fluorescent turn-off aptasensor was developed for detecting viable Cronobacter sakazakii in powdered infant formula. The sensor showed good performance and specificity in detecting the pathogen within 3 hours in both pure culture and formula samples.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Microbiology
Xin Gan, Menghan Li, Shaofei Yan, Xiaofei Wang, Wei Wang, Fengqin Li
Summary: The study found that Cronobacter sakazakii from powdered infant formula manufacturing factories in China are mostly pan-susceptible with weak biofilm-forming abilities. The isolates, mostly ST64, exhibited clinical relevance and shared a close genetic background, indicating possible cross-contamination events between processing rooms and external environments.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Yuan-song Zhang, Xuechao Xu, Juanli Yang, Mengyuan Tan, Wenyuan Zhou, Lu Gao, Zhen-quan Yang
Summary: In this study, a chromogenic system based on phage-directed immobilized nanozyme was developed for colorimetric measurement of Cronobacter sakazakii in powdered infant formula (PIF). The method showed high specificity and low detection limit, successfully detecting C. sakazakii and achieving good recovery rates.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Jae-Ik Lee, Sang-Soon Kim, Dong-Hyun Kang
Summary: In this study, the effect of desiccation stress on the transcriptomic and phenotypic changes of Cronobacter sakazakii ATCC 29544 was investigated. The addition of glucose or lactose to the growth medium induced differentially expressed genes (DEGs) and altered the membrane lipids, making C. sakazakii more susceptible to lipid oxidation. The transcriptome and phenotype of C. sakazakii were found to be influenced by the nutritional materials used to cultivate the pathogen.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Zhanwen Zheng, Yawen Xie, Sheng Ma, Junhong Tu, Jiahui Li, Sen Liang, Yunfeng Xu, Chao Shi
Summary: The study demonstrated that 405-nm LED light effectively inactivated C. sakazakii in PIF, reducing its tolerance to various environmental stressors. LED illumination also downregulated the transcription levels of tolerance-associated genes and caused damage to bacterial cell membranes. This suggests the potential of 405-nm LED technology in preventing and controlling pathogens in food processing and storage environments.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Food Science & Technology
Geng Zou, Libere Ndayishimiye, Lingxiang Xin, Manshan Cai, Longjian Zhang, Jie Li, Zhiyong Song, Renwei Wu, Yang Zhou, Yuanguo Shi, Yingwang Ye, Rui Zhou, Jinquan Li
Summary: In this study, a phage termed LPCS28 with a broad lysis spectrum against C. sakazakii was isolated from environmental water samples. LPCS28 showed high thermal stability, with no significant decrease in titer after heating at 60 degrees C for one hour. Upon thermal processing at 63 degrees C for 30 min, LPCS28 effectively inhibited C. sakazakii in reconstituted powdered infant formula and liquid milk, reducing its concentration to below the limit of detection within 9 h at 37 degrees C. Phylogenetic analysis classified LPCS28 as a new genus, Nanhuvirus, within the family Straboviridae. These results highlight the potential of LPCS28 as a biological control agent against pathogenic C. sakazakii in the dairy industry.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Lihan Wang, Xinyi Pang, Jiayuan Zhao, Haonan Jin, Xinyan Yang, Shiqian Fu, Shasha Cheng, Hongxuan Li, Chao Miao, Chaoxin Man, Yujun Jiang
Summary: JK004 is a novel virulent phage isolated from the native environment of C. sakazakii, known for its tolerance to heat, pH, and osmotic-stress. It shows excellent antibacterial activity against C. sakazakii and biofilms, without containing any known virulence, transduction, or antibiotic resistance genes, indicating its safety for practical applications in food production. The study suggests the potential of JK004 as an effective strategy for controlling C. sakazakii.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Food Science & Technology
Rajni Chauhan, Ben D. Tall, Gopal Gopinath, Wamik Azmi, Gunjan Goel
Summary: This article reviews the factors and strategies responsible for the survival and persistence of Cronobacter sakazakii in powdered infant formula (PIF), focusing on thermal resistance, antibiotic or antimicrobial resistance, virulence potential, and stress-associated gene expression. This pathogen poses a high risk to neonates and its ability to survive in low-moisture foods is influenced by intrinsic and extrinsic factors.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Food Science & Technology
Yanpeng Yang, Sheng Ma, Kailun Guo, Du Guo, Jiahui Li, Muxue Wang, Yutang Wang, Chunling Zhang, Xiaodong Xia, Chao Shi
Summary: This study found that combined LED illumination and citral treatment can effectively reduce the abundance of C. sakazakii in reconstituted powdered infant formula. The combination treatment also destroys cellular morphology and membrane integrity, and induces lipid peroxidation. Neither LED illumination nor citral treatment results in DNA breakdown in C. sakazakii.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
Ondrej Holy, Julio Parra-Flores, Sarah Lepuschitz, Maria Paula Alarcon-Lavin, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Jetsi Mancilla-Rojano, Werner Ruppitsch, Stephen Forsythe
Summary: This study investigated the putative virulence factors of six Cronobacter sakazakii strains isolated from powdered milk, identifying different sequence types and resistance genes. Some strains demonstrated high adherence and invasion abilities, while the cpa gene was not detected. Various virulence and resistance genes, as well as plasmids, were identified through whole-genome sequencing. Additionally, CRISPR matrices were observed in all strains, enhancing decision-making for health authorities to protect public health.
Article
Microbiology
Julio Parra-Flores, Ondrej Holy, Francisca Riffo, Sarah Lepuschitz, Eduard Maury-Sintjago, Alejandra Rodriguez-Fernandez, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Jetsi Mancilla-Rojano, Miriam Troncoso, Guillermo Figueroa, Werner Ruppitsch, Stephen Forsythe
Summary: This study used whole genome sequencing and laboratory studies to characterize Cronobacter strains from contaminated products, revealing multiple antibiotic resistance and virulence genes, supporting the decision to recall contaminated powdered and dairy formulas from the Chilean market in 2017.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Julio Parra-Flores, Ondrej Holy, Francisca Riffo, Sarah Lepuschitz, Werner Ruppitsch, Stephen Forsythe
Summary: Cronobacter sakazakii is a pathogen that causes severe diseases in infants under 12 months, such as meningitis and necrotizing enterocolitis. Seven C. sakazakii genome sequences isolated from PIF and dairy products in Chile in 2017 are presented.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Microbiology
Julio Parra-Flores, Ondrej Holy, Fernanda Bustamante, Sarah Lepuschitz, Ariane Pietzka, Alejandra Contreras-Fernandez, Claudia Castillo, Catalina Ovalle, Maria Paula Alarcon-Lavin, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Jetsi Mancilla-Rojano, Miriam Troncoso, Guillermo Figueroa, Werner Ruppitsch
Summary: This study investigated the virulence factors and antibiotic resistance in Listeria monocytogenes strains isolated from ready-to-eat foods. The results showed that these strains carried virulence and antibiotic resistance traits that pose a potential public health risk for consumers.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Nutrition & Dietetics
Eduard Maury-Sintjago, Alejandra Rodriguez-Fernandez, Julio Parra-Flores, Marcela Ruiz-De la Fuente
Summary: The aim of the study was to determine the association between menstrual cycle phase and resting metabolic rate (RMR) as well as dietary intake. The results showed that lean women had increased RMR, caloric intake, and lipid intake during the luteal phase, while women with obesity only had increased carbohydrate intake.
Article
Environmental Sciences
Alejandra Rodriguez-Fernandez, Natalia Ramos-Castillo, Marcela Ruiz-De la Fuente, Julio Parra-Flores, Eduard Maury-Sintjago
Summary: Gestational exposure to PM2.5 and PM10 particulate matter is associated with prematurity and low birth weight in Chilean newborns. PM10 affects the entire pregnancy, while PM2.5 is primarily associated with low birth weight in the second trimester.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Nutrition & Dietetics
Alejandra Rodriguez-Fernandez, Ximena Sanhueza-Riquelme, Gloria Carcamo-Vargas, Julio Parra-Flores, Ana Lizette Rojas-Rodriguez, Marcela Ruiz-De la Fuente, Eduard Maury-Sintjago
Summary: There is limited knowledge regarding the factors influencing non-exclusive breastfeeding (NEBF) practices in immigrant populations, particularly in Latin America. This study aimed to determine the association between maternal factors and the prevalence of NEBF in Haitian immigrant women in southern Chile. The findings showed that Haitian immigrant women without legal residency, recently arrived, with low educational attainment, and limited knowledge of breastfeeding are at higher risk of not providing exclusive breastfeeding. However, employment, access to basic services, and Spanish proficiency were found to be protective factors associated with higher rates of exclusive breastfeeding.
Article
Pediatrics
Alejandra Rodriguez-Fernandez, Marcela Ruiz-De la Fuente, Ximena Sanhueza-Riquelme, Julio Parra-Flores, Maria Dolores Marrodan, Eduard Maury-Sintjago
Summary: The study found that maternal factors, such as educational attainment and age, are associated with preterm and low-birthweight births in Chile. Mothers with less than 12 years of education and mothers aged 35 or older were found to be at a higher risk for preterm and low-birthweight newborns.
Article
Food Science & Technology
Julio Parra-Flores, Adriana Cabal-Rosel, Beatriz Daza-Prieto, Pamela Chavarria, Eduard Maury-Sintjago, Alejandra Rodriguez-Fernandez, Sergio Acuna, Werner Ruppitsch
Summary: This study genetically characterized Enterobacteriaceae and Enterococcus strains isolated from powdered infant formulas (PIF). The strains exhibited resistance to multiple antibiotics and carried various resistance and virulence genes. These multidrug-resistant strains pose a health risk for infants consuming PIF.
Article
Infectious Diseases
Ondrej Holy, Julio Parra-Flores, Jaroslav Bzdil, Adriana Cabal-Rosel, Beatriz Daza-Prieto, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Ricardo Rodriguez-Martinez, Sergio Acuna, Stephen Forsythe, Werner Ruppitsch
Summary: The objective of this study was to screen for antibiotic resistance, fitness and virulence genes in Cronobacter sakazakii strains isolated from food and powdered-milk-producing environments using whole-genome sequencing. Antibiotic-resistance genes and virulence genes were detected using various tools, and susceptibility testing was performed. The strains showed resistance to cephalotin and ampicillin, and multiple ARGs and VGs were identified. These findings suggest a potential risk of infection in susceptible populations.
Article
Food Science & Technology
Elaine Benitez-Correa, Jose Miguel Bastias-Montes, Sergio Acuna-Nelson, Ociel Munoz-Farina
Summary: The extraction kinetics of polyphenols from cocoa bean shells using deep eutectic solvents and their effects on antioxidant capacity and main compounds were analyzed. The results showed that the type of hydrogen bond donor influenced the properties of the extracts, and an additional mechanism enhanced the extraction capacity of deep eutectic solvents compared with organic solvents.
CURRENT RESEARCH IN FOOD SCIENCE
(2023)
Article
Chemistry, Physical
Mauricio Opazo-Navarrete, Cesar Burgos-Diaz, Karla A. Garrido-Miranda, Sergio Acuna-Nelson
Summary: This study aimed to improve the solubility and emulsifying properties of lupin proteins through enzymatic hydrolysis. The results showed that neutral and alkaline proteases were more efficient in hydrolyzing lupin proteins. The solubility and emulsifying capacity of hydrolyzed lupin proteins were significantly improved compared to untreated proteins.
COLLOIDS AND INTERFACES
(2022)
Article
Biotechnology & Applied Microbiology
Gemechu G. Abdi, Yetenayet B. Tola, Chala G. Kuyu
Summary: This study evaluated the quality of honey in the supply chain in southwest Ethiopia and found significant differences in physicochemical and microbial quality among different actors. The study also developed a predictive model to detect adulteration, which showed good linearity and predictive capacity.
JOURNAL OF FOOD PROTECTION
(2024)
Article
Biotechnology & Applied Microbiology
Kathleen A. Glass, Jie Yin Lim, Quinn L. Singer
Summary: Commercial cheese brines can be a reservoir for salt-tolerant pathogens. This study found that the addition of hydrogen peroxide can effectively reduce the population of L. monocytogenes in cheese brines, especially at higher temperatures and salt concentrations. The presence of indigenous microorganisms may neutralize the effect of hydrogen peroxide.
JOURNAL OF FOOD PROTECTION
(2024)