4.7 Article

Sugar recovery of enzymatic hydrolysed oil palm empty fruit bunch fiber by chemical pretreatment

Journal

CELLULOSE
Volume 20, Issue 6, Pages 3191-3203

Publisher

SPRINGER
DOI: 10.1007/s10570-013-0033-1

Keywords

Empty fruit bunch fiber (EFBF); Pretreatment; Enzymatic sugar recovery; Black liquor

Funding

  1. Ministry of Science, Technology and Innovation (MOSTI) [10-05-MGI-GMB001]
  2. Industrial Biotechnology Scheme [DIP-2012-018]

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An investigation was conducted to study the effect of solvent composition and temperature on the efficiency of pretreatment prior to enzymatic hydrolysis. The aim was to improve the sugar recovery of oil palm empty fruit bunch fiber (EFBF) through enzymatic hydrolysis. Two types of pretreatments, namely, acidified-glycerol (AC-g) pretreatment and alkaline-glycerol (AL-g) pretreatment were conducted. The study proved that AL-g pretreatment promoted higher delignification and enzymatic hydrolyzed sugar yield compared to AC-g pretreatment. Total sugar recovery of 81.44 and 96.55 % was achieved from AL-g pretreatment at 80 and 120 A degrees C respectively, following the enzymatic hydrolysis. However, downstream industrial processes, involving enzyme treatment along the processing line have the preference of acidic condition. Thus, AC-g pretreatment was favorable. Approximately 51.74 % total sugar had been recovered successfully from enzymatic hydrolysis of EFBF after 3 h of pretreatment by using solvent comprising of 50 % acetic acid and 80 % aqueous glycerol at a ratio of 97:3 at 120 A degrees C.

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