Article
Chemistry, Analytical
Tao Zhao, Jing-Wen Wang, Hong-Shuai Zhang, Xin Zheng, Yi-Ping Chen, Hao Tang, Jian-Hui Jiang
Summary: This study presents a dual-nanopore biosensor for the detection of dopamine at the single-cell level. The biosensor allows rapid and specific detection of dopamine, as well as monitoring changes in dopamine concentration within individual cells.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Min Hye Kang, Yun-Jeong Ji, Yu Mi Han, Gwi Yeong Jang, Dong Hwi Kim, Jeong Hoon Lee, Geum-Soog Kim, Su Ji Choi, Hyung Don Kim
Summary: The study found that Sasa borealis has high antioxidant activity, can effectively reduce ROS generation, promote the expression of a range of antioxidant enzymes, and shows potential for preventing neurodegenerative diseases.
APPLIED SCIENCES-BASEL
(2022)
Article
Pharmacology & Pharmacy
Sergejs Zavadskis, Adelheid Weidinger, Dominik Hanetseder, Asmita Banerjee, Cornelia Schneider, Susanne Wolbank, Darja Marolt Presen, Andrey Kozlov
Summary: The study found that the inhibitor DPI has different effects on ROS metabolism and mitochondrial function in various types of cells, such as stem cells and differentiated cells, depending on the cell's energy metabolism. Undifferentiated cells may be a better target for DPI compared to differentiated parenchymal cells.
Article
Chemistry, Applied
Serdal Kaya, Yahya A. Ismaiel, Nahyun Kwon, Gyoungmi Kim, Jose L. Bila, Juyoung Yoon, Ozlem Seven, Engin U. Akkaya
Summary: Singlet oxygen is a cytotoxic reactive species involved in cancer photodynamic therapy. Bodipy-based fluorescent dyes with singlet oxygen reactive modules can signal the intracellular generation of singlet oxygen through photosensitization. Long wavelength probes of singlet oxygen based on this approach are expected to be highly valuable.
Article
Neurosciences
Wenjun Zhao, Lei Dong, Lei Tian, Ling Zhao, Yuhan Zhao, Yu Zheng
Summary: This study investigates the regulation of 15 Hz/2 mT ELF-EMFs on long-term potentiation (LTP) persistence in the hippocampal CA1 region of Sprague-Dawley rats at different early developmental stages. The results show that ELF-EMFs differentially inhibit LTP persistence depending on the age, with a stronger inhibitory effect in younger rats. The inhibitory effect is associated with intracellular calcium stores mediated by inositol-1,4,5-trisphosphate receptors (IP(3)Rs), and can be modulated by adjusting extracellular calcium concentration.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2023)
Review
Cell Biology
Mojdeh Barati, Behrad Darvishi, Mohammad Amin Javidi, Ali Mohammadian, Seyed Peyman Shariatpanahi, Mohammad Reza Eisavand, Alireza Madjid Ansari
Summary: The induction of apoptosis in exposed cells by ELF-EMF is controversial, with studies showing conflicting results. The cellular stress response following ELF-EMF exposure may play a critical role in modulating apoptotic effects, highlighting the key factor influencing discrepancies in observations.
CELL PROLIFERATION
(2021)
Article
Chemistry, Multidisciplinary
Katarzyna Balon, Benita Wiatrak
Summary: Models based on cell cultures are useful tools in modern scientific research, with neoplasm-derived cell lines like PC12 and THP-1 offering flexibility for neurobiological studies but requiring prior differentiation. Proper differentiation of neoplastic cell lines is important for neurobiological research, as demonstrated by the impact on sensitivity to harmful factors such as A beta(25-35) or lipopolysaccharide.
APPLIED SCIENCES-BASEL
(2021)
Article
Neurosciences
Wen-Bo Chen, Yu-Xiang Wang, Hong-Gang Wang, Di An, Dan Sun, Pan Li, Tao Zhang, Wan-Ge Lu, Yan-Qiang Liu
Summary: This study aimed to investigate the role of intracellular zinc ion dysregulation in mediating A ss-related neurotoxicity. The results showed that the -Zn2+-specific chelator TPEN attenuated A ss 25-35-induced neurotoxicity and restored intracellular -Zn2+ concentration by modulating abnormal -Ca2+-related signaling pathways.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Lester Uy Vinzons, Shu-Ping Lin
Summary: Successful fabrication of photosensitive substrates with hierarchical structures using a simple and cost-effective method allows for the control of neuronal cell neurite outgrowth and alignment.
ACS APPLIED NANO MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Baojin Ma, Shi Guo, Yuta Nishina, Alberto Bianco
Summary: The reaction between graphene oxide and glutathione affects the intracellular redox balance, increasing reactive oxygen species levels and inhibiting cell viability and proliferation.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Medicine, Research & Experimental
Caio Pupin Rosa, Thiago Caetano Andrade Belo, Natalia Cristina de Melo Santos, Evandro Neves Silva, Juciano Gasparotto, Patricia Paiva Corsetti, Leonardo Augusto de Almeida
Summary: Intracellular production of reactive oxygen species (ROS) plays a significant role in both the lethal effects on microorganisms and the regulation of inflammation and metabolism. Various microorganisms have been found to modulate the assembly or disruption of inflammasomes through ROS production. This process is a significant milestone in our understanding of the immunology of intracellular pathogens.
Article
Cell Biology
Michiko Tashiro, Masato Konishi, Makino Watanabe, Utako Yokoyama
Summary: The intracellular concentration of free Mg2+ ([Mg2+]i) plays a crucial role in cellular functions. Reactive oxygen species (ROS) can affect [Mg2+]i homeostasis and lead to cellular damage. This study demonstrates that ROS decrease intracellular Mg2+ levels in cardiomyocytes through a Na+-independent Mg2+ efflux system, potentially contributing to ROS-induced cardiac dysfunction.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Immunology
Iwona Niemietz, Kelly L. Brown
Summary: This study found that hyaluronan (HA) can prime neutrophils to produce a stronger oxidative burst in response to different stimuli, when combined with TNF alpha. Additionally, HA can enhance intracellular reactive oxygen species (ROS) production induced by TNF alpha, as well as activate p38 MAPK and apoptosis.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Yinglu Chen, Xiaoheng He, Qiuhong Chen, Yi He, Fangman Chen, Chao Yang, Liang Wang
Summary: Fighting intracellular bacteria with strong antibiotics has been a challenge, but utilizing nanomaterials with unique physicochemical properties offers potential for precise drug delivery and modulation of the infectious microenvironment. This review explores the characteristics of intracellular infection microenvironment and how nanomaterials' properties affect their interactions with cells and bacteria. The progress of nanomaterial-based targeted delivery and controlled release of antibiotics is discussed, with an emphasis on the unique intrinsic properties of nanomaterials for treating intracellular bacteria. Opportunities and challenges of bioactive nanomaterials in addressing intracellular infections are also examined.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Oncology
Hong-Gui Xu, Viktor Reshetnikov, Marit Wondrak, Lisa Eckhardt, Leoni A. Kunz-Schughart, Christina Janko, Rainer Tietze, Christoph Alexiou, Hannes Borchardt, Achim Aigner, Wenjie Gong, Michael Schmitt, Leopold Sellner, Steffen Daum, Huelya Gizem Oezkan, Andriy Mokhir
Summary: The study explores a novel N-alkylaminoferrocene-based prodrug that serves as a radiosensitizer by enhancing reactive oxygen species (ROS) levels specifically in cancer cells, affecting mitochondrial function, while sparing normal cells. By targeting mitochondria and inducing ROS simultaneously, the prodrug efficiently enhances the efficacy of radiotherapy and demonstrates a synergistic effect between radiotherapy and ROS amplification using NAAF-based compounds.
Article
Cell Biology
Simone Guarnieri, Caterina Morabito, Michele Bevere, Paola Lanuti, Maria A. Mariggio
Summary: The study found that simulated microgravity slowed cell proliferation and decreased metabolic activity, along with increased levels of intracellular reactive oxygen species and calcium ions. The presence of an antioxidant, N-acetylcysteine, counteracted the effects of simulated microgravity.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2021)
Review
Biochemistry & Molecular Biology
Rosa Mancinelli, Franco Checcaglini, Francesco Coscia, Paola Gigliotti, Stefania Fulle, Giorgio Fano-Illic
Summary: In the last decade, evidence has emerged showing the importance of myokines released from skeletal muscle in impacting various biological functions. Specific myokines such as irisin have unique roles in modulating oxidative stress. Understanding the role of myokines in skeletal muscle aging processes can help in comprehending the regulatory mechanisms of muscle cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Michele Bevere, Caterina Morabito, Maria A. Mariggio, Simone Guarnieri
Summary: This review provides an overview of the key hallmarks of cardiomyocytes in physiological and pathological conditions, highlighting the metabolic aspects, cellular structures, intracellular calcium homeostasis, and intracellular zinc balance. These components are strongly linked to each other through intracellular ROS management, and their imbalance can lead to cardiovascular diseases.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Biochemistry & Molecular Biology
Guya Diletta Marconi, Ylenia Della Rocca, Luigia Fonticoli, Simone Guarnieri, Simone Carradori, Thangavelu Soundara Rajan, Jacopo Pizzicannella, Francesca Diomede
Summary: This study investigated the protective role of carvacrol (CAR) in the inflammatory process by modulating the TLR4/NF kappa B/NALP3/IL-1 beta pathway and ROS production. CAR demonstrated significant anti-inflammatory effects by reducing TLR4, NF kappa B, NALP3, and IL-1 beta expression, and also showed protective effects against ROS. CAR could potentially be used as a therapeutic agent against inflammatory stimuli.
Review
Biochemistry & Molecular Biology
Ester Sara Di Filippo, Franco Checcaglini, Giorgio Fano-Illic, Stefania Fulle
Summary: In order to respond to changes in the environment and carry out necessary metabolic functions, cells require a functional control center. This review identifies the mitochondrion as such a control center, where messengers like H2O2, Ca2+, and Zn2+ collaborate to activate adaptive response mechanisms. By exchanging information, these messengers generate a holistic response of the cell.
Article
Cell Biology
Michele Bevere, Caterina Morabito, Simone Guarnieri, Maria A. Mariggio
Summary: This study investigated the impact of GAP43 on cardiac tissue and found that its absence led to morphological changes and signs of hypertrophy in the hearts of knockout mice. This suggests that GAP43 may play a significant role in the functional processes of cardiac muscle.
HISTOCHEMISTRY AND CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Francesco Avolio, Stefano Martinotti, Vladimir Kh Khavinson, Jessica Elisabetta Esposito, Giulia Giambuzzi, Antonio Marino, Ekaterina Mironova, Riccardo Pulcini, Iole Robuffo, Giuseppina Bologna, Pasquale Simeone, Paola Lanuti, Simone Guarnieri, Svetlana Trofimova, Antonio Domenico Procopio, Elena Toniato
Summary: This study evaluates the effects of five different peptides on human monocytic THP-1 cells, revealing their ability to modulate cell proliferation patterns, reduce inflammatory responses, and enhance anti-inflammatory actions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Biochemistry & Molecular Biology
Giorgio Fano-Illic, Stefania Fulle
Editorial Material
Chemistry, Multidisciplinary
Maria A. Mariggio, Giulia Ricci
APPLIED SCIENCES-BASEL
(2022)
Article
Medicine, General & Internal
Francesco Coscia, Rosa Mancinelli, Paola Virginia Gigliotti, Franco Checcaglini, Giorgio Fano-Illic
Summary: This observational study examines the effects of physical activity on fatigue perception, maximum power output, sleep, and cognitive changes in long COVID-19 subjects. The results show that active subjects have significantly reduced fatigue perception compared to sedentary ones. Additionally, both active and sedentary subjects experienced reduced strength development, poor sleep quality, and mild cognitive impairment.
Article
Cell Biology
Marika Berardini, Luisa Gesualdi, Caterina Morabito, Francesca Ferranti, Anna Reale, Michele Zampieri, Katsiaryna Karpach, Antonella Tinari, Lucia Bertuccini, Simone Guarnieri, Angela Catizone, Maria A. Mariggio, Giulia Ricci
Summary: The study shows that simulated microgravity exposure can lead to accumulation of reactive oxygen species in TCam-2 cells, along with increased protein and lipid oxidation, and altered mitochondria structure. However, the cells are capable of triggering compensatory mechanisms to overcome the effects of altered gravitational force.
