Journal
CELLULAR IMMUNOLOGY
Volume 290, Issue 2, Pages 190-195Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cellimm.2014.06.006
Keywords
Sepsis; miR-27a; TNF-alpha; IL-6; Inflammatory response; NF-kappa B p65
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Funding
- National Natural Science Foundation of China [81272144]
- Science and Technology Commission of Shanghai Municipality [12ZR1419800]
- National Natural Science Foundation of China [81272144]
- Science and Technology Commission of Shanghai Municipality [12ZR1419800]
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MicroRNAs (miRNAs) are short, non-coding RNAs that regulate the expression of multiple target genes. Dysregulation of miRNAs is common in sepsis. Through microRNA microarray and qRT-PCR we found that the levels of miR-27a, miR-153 and miR-143 are up regulated, while let-7a, miR-218 and miR-129-5p are down regulated in lungs of septic mice. Knocking down of miR-27a down regulates expression levels of TNF-alpha and IL-6 significantly via reducing the phosphorylation level of NF-kappa B p65 and inhibiting its DNA binding activity. Furthermore, neutralisation of miR-27a up regulates PPAR gamma level, down regulates TNF-alpha expression, relieves pulmonary inflammation and promotes survival of septic mice, which demonstrates that miR-27a plays an important role in regulating inflammatory response in sepsis and provides a potential target for clinical sepsis research and treatment. (C) 2014 Elsevier Inc. All rights reserved.
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