Article
Genetics & Heredity
Chiao-Yun Lin, Chia-Lung Tsai, Angel Chao, Li-Yu Lee, Wei-Chun Chen, Yun-Hsin Tang, An-Shine Chao, Chyong-Huey Lai
Summary: Despite recent therapeutic breakthroughs, advanced and/or recurrent endometrial cancer remains a significant health burden globally. Our study suggests that NPM/B23-induced CD24 expression in endometrial cancer cells plays a role in immune evasion by inhibiting macrophage-mediated phagocytosis. CD24 may serve as a novel target for endometrial cancer immunotherapy.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2021)
Article
Multidisciplinary Sciences
Yifeng Qi, Bin Zhang
Summary: Nuclear bodies are membraneless condensates that may form via liquid-liquid phase separation; however, they defy theoretical predictions where the equilibrium state should consist of a single droplet. Using a diploid human genome model parameterized with Hi-C data, simulations suggest that the chromatin network allows for the existence of multiple droplets.
NATURE COMMUNICATIONS
(2021)
Editorial Material
Cell Biology
Guang Xu, Man Wu, Ling-Ling Chen
Summary: This study identifies a nucleolar-localized long noncoding RNA (lncRNA), LETN, as essential for tumor cell proliferation through its interaction with NPM1, highlighting the importance of this mutually dependent relationship in maintaining nucleolar function and sustaining cancer cell proliferation.
Review
Biochemistry & Molecular Biology
Hazel C. Thoms, Lesley A. Stark
Summary: The nucleolus plays a critical role in stress response and cellular homeostasis regulation, affecting the signaling pathways of p53 and NF-kappa B. This review delves into the NF-kappa B nucleolar stress response pathway, discussing apoptosis, NF-kappa B signaling activation, and the role of aspirin in inducing apoptosis of colon cancer cells.
Article
Cell Biology
Lilian C. Russo, Pault Y. M. Ferruzo, Fabio L. Forti
Summary: DUSP3 interacts with NPM in the cell nucleus after UV stress, showing high-affinity binding and dephosphorylating specific tyrosines of NPM. This interaction affects HDM2-p53 interaction in the nucleoplasm, leading to increased stability and transcriptional activity of p53, favoring DNA repair and genomic stability.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Optics
M. S. Pochechuev, A. A. Lanin, I. Kelmanson, A. S. Chebotarev, E. S. Fetisova, D. S. Bilan, E. K. Shevchenko, A. A. Ivanov, A. B. Fedotov, V. V. Belousov, A. M. Zheltikov
Summary: Multimodal nonlinear microscopy combining third-harmonic generation (THG) with two- and three-photon excited fluorescence (2PEF and 3PEF) provides a powerful resource for high-fidelity imaging of nucleoli and nucleolar proteins. With a suitably tailored genetically encoded fluorescent stain, reliable detection of specific nucleolar proteins' 2PEF/3PEF readout against extranucleolar signal enables high-contrast imaging. THG offers a versatile readout for imaging unstained nucleoli in various biological systems, from neurons in brain slices to cultured HeLa cells.
Article
Cell Biology
Xianteng Wang, Xiaolin Hu, Wanlu Song, Hui Xu, Zhengtao Xiao, Rongyao Huang, Qingran Bai, Fan Zhang, Yongzhen Chen, Yu Liu, Jianhuo Fang, Xin Li, Qin Shen, Haitao Zhao, Xuerui Yang
Summary: The nucleolus is a crucial organelle for fundamental cellular processes, regulated by complex mechanisms. This study uncovers a novel functional dependency between a long non-coding RNA and a nucleolar protein, affecting cell proliferation and nucleolar functions. This human-specific protein-lncRNA axis reveals a critical layer of regulation in cancer and normal developmental processes.
Article
Oncology
Eric J. Lowe, Anne F. Reilly, Megan S. Lim, Thomas G. Gross, Lauren Saguilig, Donald A. Barkauskas, Rui Wu, Sarah Alexander, Catherine M. Bollard
Summary: The ANHL12P1 clinical trial demonstrated that the addition of CZ to standard treatment prevented relapses during therapy for children with nonlocalized ALK+ CD30+ ALCL, MDD predicted EFS, and the addition of CZ resulted in unexpected thromboembolic events.
JOURNAL OF CLINICAL ONCOLOGY
(2023)
Article
Oncology
Gavin D. Garland, Stephen P. Ducray, Leila Jahangiri, Perla Pucci, G. A. Amos Burke, Jack Monahan, Raymond Lai, Olaf Merkel, Ana-Iris Schiefer, Lukas Kenner, Andrew J. Bannister, Suzanne D. Turner
Summary: Research has shown that nucleophosmin-anaplastic lymphoma kinase (NPM-ALK) drives anaplastic large cell lymphoma (ALCL) by regulating another protein, BRG1, to promote tumor cell proliferation. Inactivation of the gene that leads to expression of BRG1 results in tumor cell death, suggesting that therapeutic targeting of BRG1 may be a novel therapy for this form of cancer.
Article
Biochemistry & Molecular Biology
Takaaki Yasuhara, Yu-Hang Xing, Nicholas C. Bauer, Lukuo Lee, Rui Dong, Tribhuwan Yadav, Roy J. Soberman, Miguel N. Rivera, Lee Zou
Summary: The proper function of the genome relies on the spatial organization of DNA, RNA, and proteins. Research shows that transcription inhibition leads to the formation of condensates (CITIs) that drastically alter the genome's spatial organization. Additionally, proper transcription and RNA processing prevent the generation of gene fusions caused by DNA double-strand breaks (DSBs).
Article
Biochemistry & Molecular Biology
Marlena Cichocka, Anna Karlik, Patrycja Plewka, Kishor Gawade, Agata Stepien, Patrycja Swiergiel, Ankur Gadgil, Katarzyna Dorota Raczynska
Summary: hnRNP UL1 plays multiple important roles in the cell nucleus, including involvement in DNA double-strand break repair, stimulation of ribosomal DNA gene transcription, and interaction with various related proteins.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Management
Elin K. Funck, Tom S. Karlsson
Summary: The effects of NPM have faced criticism for a long time, and now alternative, post-NPM governance innovations are emerging. These innovations share a critique of NPM and a desire to provide new ways of governing public organizations. We argue that instead of demonizing NPM, a more productive approach would be to incorporate elements of NPM into a holistic governance model for the future.
PUBLIC MANAGEMENT REVIEW
(2023)
Article
Cell Biology
Aaron Rodrigues, Kyle L. MacQuarrie, Emma Freeman, Alicia Lin, Alexander B. Willis, Zhaofa Xu, Angel A. Alvarez, Yongchao Ma, Bethany E. Perez White, Daniel R. Foltz, Sui Huang
Summary: This study investigated the dynamic changes in nucleolar characteristics and nucleoli-centromere interactions under different physiological and pathological conditions. The results showed that as cells differentiate, the nucleolar number increases and the centromere association decreases. In cancer cells, the pattern of nucleoli number and nucleoli-centromere association is similar to proliferative cell types, suggesting that nucleolar reorganization may play a role in facilitating malignant transformation. These findings highlight the active roles of nucleolar structure in centromere function and genome organization critical for both normal development and cancer.
MOLECULAR BIOLOGY OF THE CELL
(2023)
Article
Cell Biology
Hazel Mangan, Brian McStay
Summary: The distribution of ribosomal gene arrays from multiple chromosomal nucleolar organizers within human nucleoli is not fully understood. By genetically manipulating individual NORs, customized functional ribosomes can be efficiently produced. Ribosome biogenesis is shown to occur within constrained territories, tethered to individual NORs inside the nucleolus.
GENES & DEVELOPMENT
(2021)
Article
Cell Biology
Cristiana Bersaglieri, Raffaella Santoro
Summary: The nucleolus is an important subcompartment in the nucleus responsible for ribosome biogenesis. Recent studies have shown that it also plays a role in organizing the chromosomes in the nucleus. These genomic domains, called nucleolar associated domains (NADs), are characterized by repressive chromatin states. However, accurate identification of NADs has been challenging due to the lack of a membrane.
CURRENT OPINION IN CELL BIOLOGY
(2023)
Article
Virology
Gabriele Stakaityte, Nnenna Nwogu, Samuel J. Dobson, Laura M. Knight, Christopher W. Wasson, Francisco J. Salguero, David J. Blackbourn, G. Eric Blair, Jamel Mankouri, Andrew Macdonald, Adrian Whitehouse
JOURNAL OF VIROLOGY
(2018)
Article
Pharmacology & Pharmacy
Liangliang Li, Biyun Xue, Weiyao Sun, Guoqian Gu, Gaopeng Hou, Lu Zhang, Chunyan Wu, Qin Zhao, Yanjin Zhang, Gaiping Zhang, Julian A. Hiscox, Yuchen Nan, En-Min Zhou
ANTIVIRAL RESEARCH
(2018)
Article
Immunology
Emily Speranza, Paula Ruibal, Julia R. Port, Feng Feng, Lia Burkhardt, Adam Grundhoff, Stephan Guenther, Lisa Oestereich, Julian A. Hiscox, John H. Connor, Cesar Munoz-Fontela
JOURNAL OF INFECTIOUS DISEASES
(2018)
Article
Biochemistry & Molecular Biology
Oliver Manners, Belinda Baquero-Perez, Adrian Whitehouse
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2019)
Article
Multidisciplinary Sciences
L. E. Kafetzopoulou, S. T. Pullan, P. Lemey, M. A. Suchard, D. U. Ehichioya, M. Pahlmann, A. Thielebein, J. Hinzmann, L. Oestereich, D. M. Wozniak, K. Efthymiadis, D. Schachten, F. Koenig, J. Matjeschk, S. Lorenzen, S. Lumley, Y. Ighodalo, D. I. Adomeh, T. Olokor, E. Omomoh, R. Omiunu, J. Agbukor, B. Ebo, J. Aiyepada, P. Ebhodaghe, B. Osiemi, S. Ehikhametalor, P. Akhilomen, M. Airende, R. Esumeh, E. Muoebonam, R. Giwa, A. Ekanem, G. Igenegbale, G. Odigie, G. Okonofua, R. Enigbe, J. Oyakhilome, E. O. Yerumoh, I. Odia, C. Aire, M. Okonofua, R. Atafo, E. Tobin, D. Asogun, N. Akpede, P. O. Okokhere, M. O. Rafiu, K. O. Iraoyah, C. O. Iruolagbe, P. Akhideno, C. Erameh, G. Akpede, E. Isibor, D. Naidoo, R. Hewson, J. A. Hiscox, R. Vipond, M. W. Carroll, C. Ihekweazu, P. Formenty, S. Okogbenin, E. Ogbaini-Emovon, S. Gunther, S. Duraffour
Article
Microbiology
Baoyuan Liu, Yiyang Chen, Yani Sun, Yuchen Nan, Huixia Li, Taofeng Du, Julian A. Hiscox, Qin Zhao, En-Min Zhou
VETERINARY MICROBIOLOGY
(2019)
Article
Biology
Belinda Baquero-Perez, Agne Antanaviciute, Ivaylo D. Yonchev, Ian M. Carr, Stuart A. Wilson, Adrian Whitehouse
Article
Biochemistry & Molecular Biology
Samuel J. Dobson, Anthony Anene, James R. Boyne, Jamel Mankouri, Andrew Macdonald, Adrian Whitehouse
BIOCHEMICAL JOURNAL
(2020)
Article
Virology
Nnenna Nwogu, Luz E. Ortiz, Adrian Whitehouse, Hyun Jin Kwun
JOURNAL OF VIROLOGY
(2020)
Article
Microbiology
Ethan L. Morgan, Molly R. Patterson, Emma L. Ryder, Siu Yi Lee, Christopher W. Wasson, Katherine L. Harper, Yigen Li, Stephen Griffin, G. Eric Blair, Adrian Whitehouse, Andrew Macdonald
Article
Biochemistry & Molecular Biology
Katerina Douka, Isabel Birds, Dapeng Wang, Andreas Kosteletos, Sophie Clayton, Abigail Byford, Elton J. R. Vasconcelos, Mary J. O'Connell, Jim Deuchars, Adrian Whitehouse, Julie L. Aspden
Summary: Through Poly-Ribo-Seq analysis, we identified 237 cytoplasmic lncRNAs up-regulated during early neuronal differentiation, with 45 small ORFs actively translated, 17 of which were specifically translated upon differentiation. Within these translated lncRNA-smORFs, 15 exhibit sequence conservation within Hominidea, suggesting strong selective constraint in this clade.
Review
Oncology
Katherine Louise Harper, Timothy James Mottram, Adrian Whitehouse
Summary: Circular RNAs (circRNAs) play vital roles in cellular processes, including gene expression modulation and protein interactions. Dysregulated circRNAs have a significant impact on disease progression, especially in cancer, potentially serving as novel therapeutic targets.
Article
Biochemistry & Molecular Biology
Katherine L. Harper, Timothy J. Mottram, Chinedu A. Anene, Becky Foster, Molly R. Patterson, Euan McDonnell, Andrew Macdonald, David Westhead, Adrian Whitehouse
Summary: Non-coding RNA regulatory networks play a critical role in gene expression, modulating multiple cellular pathways and impacting various diseases. A novel host ncRNA regulatory network essential for KSHV lytic replication in B cells involves KSHV-mediated upregulation of host cell circHIPK3, acting as a competing endogenous RNA of miR-30c and leading to increased levels of DLL4. Dysregulation of this network during KSHV lytic replication highlights a novel mechanism of cell cycle control.
Article
Biochemical Research Methods
Konstantin Roder, Amy M. Barker, Adrian Whitehouse, Samuela Pasquali
Summary: This article investigates the change in structure of an RNA regulatory molecule upon a change in the chemistry of one of its bases. The results show that a chemical modification can induce a global rearrangement of the structure, affecting regions far away from the modification site.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
James C. Murphy, Elena M. Harrington, Sophie Schumann, Elton J. R. Vasconcelos, Timothy J. Mottram, Katherine L. Harper, Julie L. Aspden, Adrian Whitehouse
Summary: Historically, ribosomes were thought to be homogeneous machines lacking regulatory capacity, but recent discoveries have uncovered the existence of compositionally distinct, specialized ribosomes that actively regulate mRNA translation. This study focuses on the manipulation of ribosome biogenesis by KSHV to generate specialized ribosomes for efficient translation of viral mRNAs.
NATURE COMMUNICATIONS
(2023)