Article
Cell Biology
Aleksandra Romaniuk-Drapala, Ewa Toton, Natalia Konieczna, Marta Machnik, Wojciech Barczak, Dagmar Kowal, Przemyslaw Kopczynski, Mariusz Kaczmarek, Blazej Rubis
Summary: This study investigates the impact of telomerase downregulation on breast cancer cells and reveals increased drug sensitivity, decreased proliferation, altered signaling pathways, and potential activation of autophagy. These findings provide new evidence for the therapeutic potential of telomerase downregulation in eliminating cancer cells.
Article
Biochemistry & Molecular Biology
Lygia S. Nogueira, Carolina P. Vasconcelos, Geovanni Pereira Mitre, Leonardo Oliveira Bittencourt, Jessica Rodrigues Placa, Maria Sueli da Silva Kataoka, Joao de Jesus Viana Pinheiro, Gustavo Pompermaier Garlet, Edivaldo H. C. De Oliveira, Rafael R. Lima
Summary: This study explored the global gene expression changes in hPLF cells after immortalization through the ectopic expression of hTERT, showing successful immortalization without overexpression of the hTERT gene. The results indicated that hPLF-hTERT maintained proliferation and morphology characteristics, with altered gene expression possibly regulated at the posttranscriptional level. Further studies are encouraged to design experimental protocols from a translational perspective to investigate clinical questions.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Chenjing Ma, Xian Li, Weifeng Ding, Xin Zhang, Hang Chen, Ying Feng
Summary: In this study, the effects of introducing exogenous hTERT on Periplaneta americana cells were investigated. It was found that hTERT expression promoted cell growth and extended cell lifespan by increasing telomerase activity and elongating telomeres. This suggests that introducing hTERT into insect cells could be an efficient method to establish certain insect cell lines.
Article
Health Care Sciences & Services
Aigul R. Rakhmatullina, Rimma N. Mingaleeva, Dina U. Gafurbaeva, Olesya N. Glazunova, Aisylu R. Sagdeeva, Emil R. Bulatov, Albert A. Rizvanov, Regina R. Miftakhova
Summary: This study successfully immortalized adipose-derived MSCs by overexpressing hTERT gene and knocking down TP53, maintaining the differentiation potential and cell morphology stability during long-term culture.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Genetics & Heredity
Quy Van Chanh Le, SeungYeon Youk, Munjeong Choi, Hyoim Jeon, Won-Il Kim, Chak-Sum Ho, Chankyu Park
Summary: This study generated a panel of immortalized cell lines by transfecting human telomerase reverse transcriptase (hTERT) and investigated their characteristics and genotypes, revealing their potential applications.
FRONTIERS IN GENETICS
(2022)
Article
Biochemical Research Methods
Shanhui Ren, Xue Yang, Tingyu Peng, Xusheng Qiu, Xiangwei Wang, Xiangping Yin, Xuerui Wan, Yuefeng Sun, Haotai Chen
Summary: A stable hTERT-expressing fetal cow skin fibroblast cell line was successfully established using recombinant DNA technology, which exhibited immortal properties and demonstrated susceptibility to Lumpy skin disease virus (LSDV). This cell line provides an important model for basic and applied research, clinical applications, and vaccine development.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Cell Biology
Qianyu Cheng, Chang Liu, Qiuman Chen, Wenping Luo, Tong-Chuan He, Deqin Yang
Summary: Stem cells from the apical papilla (SCAPs) are a promising candidate for tissue regeneration and regenerative endodontic treatment. However, obtaining enough cells from the limited apical papilla tissue and maintaining their primary phenotype is challenging. This study successfully immortalized human SCAPs, allowing for long-term proliferation and retaining mesenchymal and progenitor biomarkers. The immortalized cells showed greater potential for osteogenic differentiation compared to primary cells, making them a potential source for bone tissue engineering.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Daniela-Madalina Iacomi, Ana-Maria Rosca, Raluca Tutuianu, Tiberiu Paul Neagu, Vasile Pruna, Maya Simionescu, Irina Titorencu
Summary: Engineered immortalized ADSC with extended lifespan and maintained beneficial properties could be a valuable tool for wound healing and skin regenerative therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Daniel Otero-Albiol, Amancio Carnero
Summary: Cellular senescence is a complex physiological state characterized by proliferative arrest, with various functions beyond tumor suppression. Low oxygen conditions have been observed to increase the lifespan of mammalian cells and promote self-renewal and pluripotency in stem cells. Hypoxia facilitates senescence bypass, malignant transformation, and acquisition of stemness properties, contributing to tumor development and aggressiveness in cancer.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Review
Cell Biology
Ruozhu Yang, Yi Han, Xinyu Guan, Yue Hong, Jiahao Meng, Shirong Ding, Qian Long, Wenjun Yi
Summary: TERT is an essential enzyme responsible for telomere maintenance and genome stability. In breast cancer, the overactivation of hTERT plays a critical role in tumor initiation and progression. Understanding the mechanisms of hTERT activation in cancer could lead to improved understanding of the disease and the development of more effective treatment strategies.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Multidisciplinary Sciences
Melina Beykou, Mar Arias-Garcia, Theodoros I. Roumeliotis, Jyoti S. Choudhary, Nicolas Moser, Pantelis Georgiou, Chris Bakal
Summary: Palbociclib treatment can induce a senescence-like cell state in TNBC cells, providing insights for the future use of CDK4/6 inhibitors and the development of combination therapies in TNBC subtypes.
Article
Computer Science, Information Systems
Joyce A. Ayoola, Tokunbo Ogunfunmi
Summary: Advancements in computer-aided tools have improved accurate breast cancer prediction models, reducing the mortality rate. Random forest predictor and genetic algorithm are key methods in achieving high accuracy and effective feature selection. This paper proposes hybridized genetic algorithm models for breast cancer prediction, considering the order of feature selection algorithms, and compares their performance with other learning models. The hybridized Genetic Algorithm with Fisher_Score (GA + Fisher_Score) model shows promising results with 99.12% accuracy, outperforming other hybridized genetic algorithm models.
Article
Immunology
Danielle Rodrigues Cortez, Fabio Mitsuo Lima, Joao Luis Reis-Cunha, Daniella Castanheira Bartholomeu, Rolando Andre Rios Villacis, Silvia Regina Rogatto, Andre Guilherme Costa-Martins, Fernanda Sycko Marchiano, Rafaela Andrade do Carmo, Jose Franco da Silveira, Marjorie Mendes Marini
Summary: In this study, the genetic differences between two lineages of Chagas disease-causing parasite T. cruzi were analyzed using intraspecific array-based comparative genomic hybridization (aCGH). The results showed that clone D11 had more chromosomal gains and losses compared to the parental G strain. These genomic changes suggest a dynamic genome in clone D11 that responds to environmental stress by altering gene copy numbers and generating segmental aneuploidy.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Oncology
Anindita Chakrabarty, Shayantani Chakraborty, Ranjini Bhattacharya, Goutam Chowdhury
Summary: This paper focuses on chemotherapy resistance in triple negative breast cancer (TNBC) due to therapy-induced senescence (TIS). Senescent cells and the senescence-associated secretory proteome (SASP) play a role in promoting drug resistance and aggressive clones in cancer. Strategies to control TIS using evolutionary biology principles are proposed to improve therapeutic outcomes for TNBC patients.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Ana C. Garrido-Castro, Liam F. Spurr, Melissa E. Hughes, Yvonne Y. Li, Andrew D. Cherniack, Priti Kumari, Maxwell R. Lloyd, Brittany Bychkovsky, Romualdo Barroso-Sousa, Simona Di Lascio, Esha Jain, Janet Files, Ayesha Mohammed-Abreu, Max Krevalin, Colin MacKichan, William T. Barry, Hao Guo, Daniel Xia, Ethan Cerami, Barrett J. Rollins, Laura E. MacConaill, Neal Lindeman, Ian E. Krop, Bruce E. Johnson, Nikhil Wagle, Eric P. Winer, Deborah A. Dillon, Nancy U. Lin
Summary: The study compared the genomic landscapes of de novo metastatic breast cancer (dnMBC) with recurrent metastatic breast cancer (rMBC) and found MYB amplification was enriched in triple-negative dnMBC. Mutations in specific genes were more prevalent in dnMBC, and alterations associated with shorter overall survival were identified. High tumor mutational burden correlated with better overall survival in triple-negative dnMBC, suggesting potential implications for therapeutic strategies.
CLINICAL CANCER RESEARCH
(2021)
Article
Oncology
William C. Hines, Irene Kuhn, Kate Thi, Berbie Chu, Gaelen Stanford-Moore, Rocio Sampayo, James C. Garbe, Martha Stampfer, Alexander D. Borowsky, Mina J. Bissell
BREAST CANCER RESEARCH AND TREATMENT
(2016)
Article
Multidisciplinary Sciences
William C. Hines, Paul Yaswen, Mina J. Bissell
NATURE COMMUNICATIONS
(2015)
Article
Multidisciplinary Sciences
Ramray Bhat, Brian Belardi, Hidetoshi Mori, Peiwen Kuo, Andrew Tam, William C. Hines, Quynh-Thu Le, Carolyn R. Bertozzi, Mina J. Bissell
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Cell Biology
Rocio G. Sampayo, Andres M. Toscani, Matthew G. Rubashkin, Kate Thi, Luciano A. Masullo, Lanina L. Violi, Jonathon N. Lakins, Alfredo Caceres, William C. Hines, Federico Coluccio Leskow, Fernando D. Stefani, Dante R. Chialvo, Mina J. Bissell, Valerie M. Weaver, Marina Simian
JOURNAL OF CELL BIOLOGY
(2018)
Article
Cell Biology
Alexey V. Bazarov, Marjolein van Sluis, William C. Hines, Ekaterina Bassett, Alain Beliveau, Eric Campeau, Rituparna Mukhopadhyay, Won Jae Lee, Sonya Melodyev, Yuri Zaslavsky, Leonard Lee, Francis Rodier, Agustin Chicas, Scott W. Lowe, Jean Benhattar, Bing Ren, Judith Campisi, Paul Yaswen
Article
Oncology
Jason R. Pirone, Monica D'Arcy, Delisha A. Stewart, William C. Hines, Melissa Johnson, Michael N. Gould, Paul Yaswen, D. Joseph Jerry, Sallie Smith Schneider, Melissa A. Troester
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
(2012)
Article
Cell Biology
Alexey V. Bazarov, Won Jae Lee, Irina Bazarov, Moses Bosire, William C. Hines, Basha Stankovich, Agustin Chicas, Scott W. Lowe, Paul Yaswen
Article
Oncology
Kristina A. Trujillo, William C. Hines, Keith M. Vargas, Anna C. Jones, Nancy E. Joste, Marco Bisoffi, Jeffrey K. Griffith
MOLECULAR CANCER RESEARCH
(2011)
Review
Biochemistry & Molecular Biology
Mina J. Bissell, William C. Hines
Article
Multidisciplinary Sciences
William C. Hines, Alexey V. Bazarov, Rituparna Mukhopadhyay, Paul Yaswen
Article
Multidisciplinary Sciences
Kim-Vy Nguyen-Ngoc, Kevin J. Cheung, Audrey Brenot, Eliah R. Shamir, Ryan S. Gray, William C. Hines, Paul Yaswen, Zena Werb, Andrew J. Ewald
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2012)
Editorial Material
Cell Biology
William C. Hines, Ying Su, Irene Kuhn, Kornelia Polyak, Mina J. Bissell
Article
Oncology
Kimberly S. Butler, William C. Hines, Christopher M. Heaphy, Jeffrey K. Griffith
Meeting Abstract
Oncology
Lina Ding, Ying Su, Muhammad Ekram, Sung Jin Huh, Noga Bloushtain-Qimron, Sibgat Choudhury, William Hines, Jun Yao, Mina Bissell, Kornelia Polyak
MOLECULAR CANCER RESEARCH
(2018)
Article
Oncology
Rituparna Mukhopadhyay, Sylvain V. Costes, Alexey V. Bazarov, William C. Hines, Mary Helen Barcellos-Hoff, Paul Yaswen
BREAST CANCER RESEARCH
(2010)