Article
Cell Biology
Xue Bessie Su, Menglu Wang, Claudia Schaffner, Olga O. Nerusheva, Dean Clift, Christos Spanos, David A. Kelly, Michael Tatham, Andreas Wallek, Yehui Wu, Juri Rappsilber, A. Arockia Jeyaprakash, Zuzana Storchova, Ronald T. Hay, Adele L. Marston
Summary: During cell mitosis, SUMOylation plays a critical role in modulating error correction mechanisms by regulating protein interactions, leading to stable sister kinetochore biorientation and timely anaphase onset.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Sugako Ogushi, Ahmed Rattani, Jonathan Godwin, Jean Metson, Lothar Schermelleh, Kim Nasmyth
Summary: Protection of near-centromeric REC8 cohesin and sister kinetochore co-orientation depend on spindle-chromosome complexes (SCCs) in meiosis I, while being lost in meiosis II, resulting in sister chromatid disjunction and production of haploid gametes. The catalytic activity of Separase is crucial for converting kinetochores to a bi-oriented state and deprotecting cohesin in meiosis I.Selective cleavage of REC8 near kinetochores is sufficient to disrupt co-orientation in univalent chromosomes, but may require resolution of chiasmata in bivalents.
DEVELOPMENTAL CELL
(2021)
Article
Biochemistry & Molecular Biology
Aakanksha Sane, Shreyas Sridhar, Kaustuv Sanyal, Santanu K. Ghosh
Summary: Shugoshin proteins are evolutionarily conserved and play various roles in maintaining genomic stability in different cells, including regulating chromosome positioning, maintaining chromosomal separation efficiency, and serving as a component of the spindle assembly checkpoint to ensure cell stability. This study in Candida albicans reveals novel functions of Shugoshin, such as facilitating timely disassembly of the mitotic spindle and prolonging the response to improper kinetochore-microtubule attachments.
MOLECULAR MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Valentina Mengoli, Katarzyna Jonak, Oleksii Lyzak, Mahdi Lamb, Lisa M. Lister, Chris Lodge, Julie Rojas, Ievgeniia Zagoriy, Mary Herbert, Wolfgang Zachariae
Summary: This study explores the mechanisms of genome haploidization involving cohesin protection and deprotection at centromeres during meiotic divisions. It is found that APC/C activation can lead to sister centromere separation without bipolar spindle forces in yeast and mouse oocytes. Additionally, the stability of centromeric cohesin at metaphase II requires both Rec8 protection by shugoshin and separase inhibition by securin in yeast. These findings suggest that similar mechanisms preserve chromosome integrity during metaphase II in yeast and prolonged metaphase II arrest in mammalian oocytes.
Article
Biochemistry & Molecular Biology
Tahsin Kuzdere, Valentin Flury, Thomas Schalch, Vytautas Iesmantavicius, Daniel Hess, Marc Buehler
Summary: In this study, we found that H3K9 methylation undergoes changes during mitosis in Schizosaccharomyces pombe, and the lack of trimethylation of H3K9 leads to defects in chromosome segregation during mitosis. Additionally, the switch in H3K9 methylation is accompanied by phosphorylation of Clr4.
Article
Genetics & Heredity
Ting Gong, Francis J. McNally
Summary: Errors in chromosome segregation during meiosis are a major cause of aneuploidy. Crossovers are crucial for accurate chromosome alignment and partitioning, as they hold homologous chromosomes together. However, Caenorhabditis elegans zim-2 mutants with crossover defects on chromosome V have fewer dead embryos than expected, especially in males. This discrepancy can be explained by the presence of redundant mechanisms to compensate for crossover loss, a higher production of euploid gametes, and the viability and fertility of trisomy of chromosome V. Additionally, live imaging showed that univalents can segregate independently of homology.
Article
Cell Biology
Wei Ma, Jingwen Zhou, Jian Chen, Antony M. Carr, Yoshinori Watanabe
Summary: The study elucidates the molecular mechanisms of Moa1 in regulating centromeric cohesion protection by associating with Plo1 kinase to control Rec8 phosphorylation, enhancing the activity of PP2A associated with Sgo1, and preventing the cleavage of Rec8 by separase through dephosphorylation at another site.
GENES & DEVELOPMENT
(2021)
Review
Cell Biology
Masamitsu Sato, Yasutaka Kakui, Mika Toya
Summary: Meiosis is a specialized cell division process for gamete production in eukaryotes, with chromosomes and microtubules mutually influencing each other. Studies on the fission yeast Schizosaccharomyces pombe have revealed the organization of chromosomes, cytoskeleton, and cell cycle progression in mitosis and meiosis. Meiosis is strategically designed for faithful genetic material segregation and genetic diversity production through meiosis-specific and general factors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yumi Ueki, Michael A. Hadders, Melanie B. Weisser, Isha Nasa, Paula Sotelo-Parrilla, Lauren E. Cressey, Tanmay Gupta, Emil P. T. Hertz, Thomas Kruse, Guillermo Montoya, A. Arockia Jeyaprakash, Arminja Kettenbach, Susanne M. A. Lens, Jakob Nilsson
Summary: The study reveals the structure and function of the PP2A-B56-hSgo1 complex during mitosis, highlighting the essential role of a conserved pocket on the B56 regulatory subunit for hSgo1 binding and cohesion protection. Additionally, it shows that hSgo1 inhibits the binding of PP2A-B56 substrates and that PP2A-B56 dephosphorylates Cdk1 sites on hSgo1 to regulate cohesin interactions. Overall, the research provides important insights into cohesion protection during mitosis.
Article
Cell Biology
Alexandra Pyatnitskaya, Jessica Andreani, Raphael Guerois, Arnaud De Muyt, Valerie Borde
Summary: This study uncovers the functional link between crossover formation and synaptonemal complex (SC) assembly during meiotic recombination and identifies the molecular mechanism involved.
GENES & DEVELOPMENT
(2022)
Editorial Material
Cell Biology
Corinne Grey, Bernard de Massy
Summary: The study highlights the central role of the Saccharomyces cerevisiae ZMM protein Zip4 in the formation of crossovers and synapsis initiation.
GENES & DEVELOPMENT
(2022)
Article
Biochemical Research Methods
Yuxing Zhang, Lingling Yang, Kailun Fang, Qidan Li, Hongkai Xu, Yan Ren, Jin Zi, Charlie Degui Chen, Siqi Liu
Summary: This study examined the quantitative proteomics of chromosome-binding proteins (CBPs) in meiotic prophase I (MPI) in mice. The results showed that CBPs exhibited dynamic changes and formed different complexes during MPI. These complexes functioned in different categories and provided evidence for the importance of CBP complexes in MPI.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Luisa F. Bustamante-Jaramillo, Celia Ramos, Cristina Martin-Castellanos
Summary: Cyclins and CDKs play key roles in eukaryotic cell biology, coordinating physiological conditions with cell cycle progression. Meiosis is a specialized developmental program ensuring proper genetic propagation to the next generation, with meiosis-specific cyclins being widespread in evolution. Studies have shown that the meiosis-specific Crs1 cyclin in fission yeast exhibits diversified CDK functions, with roles in meiotic S-phase progression, chromosome architecture maintenance, and other key meiosis-specific events.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Stephen R. Wellard, Yujiao Zhang, Chris Shults, Xueqi Zhao, Matthew McKay, Stephen A. Murray, Philip W. Jordan
Summary: The study highlights the critical role of centriole duplication, centrosome maturation, and separation in establishing bipolar spindles during meiotic divisions. The findings show that Polo-like kinase 1 and Aurora A kinase are essential for centrosome maturation and separation, and PLK1 is required to inhibit the second round of centriole duplication until late anaphase I to ensure accurate chromosome segregation during spermatogenesis.
Review
Genetics & Heredity
Jennifer R. Gruhn, Eva R. Hoffmann
Summary: This review explores the history and mechanisms of aneuploidy in human meiosis, as well as potential future research and clinical applications.
ANNUAL REVIEW OF GENETICS
(2022)
Article
Cell Biology
Barbora Huraiova, Judit Kanovits, Silvia Bagelova Polakova, Lubos Cipak, Zsigmond Benko, Andrea Sevcovicova, Dorothea Anrather, Gustav Ammerer, Caia D. S. Duncan, Juan Mata, Juraj Gregan
Editorial Material
Genetics & Heredity
Nina Mayerova, Lubos Cipak, Juraj Gregan
TRENDS IN GENETICS
(2020)
Article
Biochemistry & Molecular Biology
Barbara Sivakova, Jan Jurcik, Veronika Lukacova, Tomas Selicky, Ingrid Cipakova, Peter Barath, Lubos Cipak
Summary: The LFQ phosphoproteomics workflow presented in this study, based on sequential Fe-IMAC phosphopeptide enrichment, strong anion exchange (SAX), and porous graphitic carbon (PGC) fractionation strategies, enabled the identification of 8353 phosphosites in the fission yeast Schizosaccharomyces pombe, with 1274 sites being newly discovered. The results demonstrate that the combination of PGC and SAX fractionation strategies significantly enhances the robustness and specificity of LFQ phosphoproteomics studies, providing valuable insights into the complexity of protein kinase functions in S. pombe.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ivana Misova, Alexandra Pitelova, Jaroslav Budis, Juraj Gazdarica, Tatiana Sedlackova, Anna Jordakova, Zsigmond Benko, Maria Smondrkova, Nina Mayerova, Karoline Pichlerova, Lucia Strieskova, Martin Prevorovsky, Juraj Gregan, Lubos Cipak, Tomas Szemes, Silvia Bagelova Polakova
Summary: In homologous recombination, the Dbl2 protein is found to be essential for regulating gene expression and participating in gene silencing pathways along with the HIRA histone chaperone complex. Furthermore, deletion of Dbl2 results in alterations in chromatin structure and levels of histone methylation.
NUCLEIC ACIDS RESEARCH
(2021)
Editorial Material
Biochemistry & Molecular Biology
Juraj Gregan
Summary: Understanding the spatial organization of genomes is crucial in cell biology, but distinguishing relationships between sister chromatids has been challenging due to limitations in sequencing-based techniques. Recent developments have provided insights into the organization of sister chromatids.
TRENDS IN BIOCHEMICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Barbora Mikolaskova, Matus Jurcik, Ingrid Cipakova, Tomas Selicky, Jan Jurcik, Silvia Bagelova Polakova, Erika Stupenova, Andrej Dudas, Barbara Sivakova, Jana Bellova, Peter Barath, Lucia Aronica, Juraj Gregan, Lubos Cipak
Summary: By studying the interactions between Nrl1 and RNA-processing proteins, as well as the regulation of Nrl1 through phosphorylation, two mechanisms involved in the regulation of the spliceosome-associated factor Nrl1 have been identified: domain-specific interactions and post-translational modification by phosphorylation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Andrea Sevcovicova, Jana Plava, Matej Gazdarica, Eva Szabova, Barbora Huraiova, Katarina Gaplovska-Kysela, Ingrid Cipakova, Lubos Cipak, Juraj Gregan
Summary: The study revealed that phosphorylation sites on the Swi5-Sfr1 complex contribute to its partially functional state during meiosis, but the functional relevance of this phosphorylation remains to be determined.
Editorial Material
Biochemistry & Molecular Biology
Lubos Cipak
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Michaela Osadska, Tomas Selicky, Miroslava Kretova, Jan Jurcik, Barbara Sivakova, Ingrid Cipakova, Lubos Cipak
Summary: Cohesin, a multi-subunit protein complex, plays important roles in various cellular processes. Recent studies have shown that cohesin's functions are not only dependent on protein-protein interactions and modifications, but also involve RNA processing factors. Mutations, changes in expression, and alterations in the interactions between cohesin and RNA processing factors can impact chromosome segregation and genome stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Tomas Selicky, Matus Jurcik, Barbora Mikolaskova, Alexandra Pitelova, Nina Mayerova, Miroslava Kretova, Michaela Osadska, Jan Jurcik, Roman Holic, Lenka Kohutova, Jana Bellova, Zsigmond Benko, Juraj Gregan, Silvia Bagelova Polakova, Peter Barath, Lubos Cipak, Ingrid Cipakova
Summary: This study demonstrates the important role of the Gpl1-Gih35-Wdr83 complex in regulating pre-mRNA splicing in the fission yeast S. pombe, through knock-out strategy, affinity purification, and yeast-two-hybrid assay.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biology
Miroslava Kretova, Tomas Selicky, Ingrid Cipakova, Lubos Cipak
Summary: Pre-mRNA splicing is a crucial process in eukaryotic cells to generate mature messenger RNAs for protein synthesis. The splicing process involves the removal of non-coding regions (introns) and joining of coding regions (exons) in pre-mRNAs. The spliceosome, composed of various proteins and factors, carries out the specific steps of splicing. Recent studies have revealed that post-translational modifications of splicing factors play significant roles in the regulation of pre-mRNA splicing, including phosphorylation, acetylation, methylation, ubiquitination, and sumoylation.
