Article
Entomology
Alberto Maria Cattaneo, Peter Witzgall, Charles A. Kwadha, Paul G. Becher, William B. Walker III
Summary: This study reveals that Drosophila suzukii may exhibit specific responses to two plant compounds and one fly-emitted compound. Among them, methyl salicylate is the most active ligand, specifically targeting a particular olfactory receptor subunit. These findings can provide insights for future development of control strategies for D. suzukii.
JOURNAL OF PEST SCIENCE
(2022)
Review
Entomology
Hao Guo, Dean P. Smith
Summary: Insects, including blood-feeding female mosquitoes, play a major role in transmitting deadly diseases. They use olfaction to locate food, mates, and hosts, but the nature of odorant plumes poses a challenge for them. In order to adapt to different concentrations and durations of stimuli, insects desensitize their olfactory system. Understanding the molecular mechanisms behind this desensitization process is important for repellents and pest control.
Article
Biochemistry & Molecular Biology
Jing-Yu Lin, Zhao Yang, Chan Yang, Ji-Xiang Du, Fan Yang, Jie Cheng, Wei Pan, Shi-Jie Zhang, Xu Yan, Jia Wang, Jin Wang, Lu Tie, Xiao Yu, Xin Chen, Jin-Peng Sun
Summary: This study revealed the mechanism of functional coupling between BmOR3 and BmOrco, including key interactions such as ionic lock and hydrophobic zipper, and demonstrated selective coupling of BmOR3 with Gi proteins, as well as recruitment of arrestin in an agonist-dependent manner. The study provided important insights into the coupling of active PRs to their downstream effectors, including coreceptors, G proteins, and arrestin.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
Shadi Jafari, Mattias Alenius
Summary: Olfactory perception is highly individualized in humans and Drosophila, with adaptation playing a key role in shaping olfactory-guided behaviors. Adaptation occurs both in the central nervous system and in the periphery, with mechanisms across different time scales influencing olfactory perception.
CELL AND TISSUE RESEARCH
(2021)
Article
Multidisciplinary Sciences
Yupeng Wang, Ying Sun, Paule V. Joseph
Summary: In humans, there are about 400 functional olfactory receptor (OR) genes that detect odors. These OR genes can be divided into tens of families. The OR genes have mainly experienced extensive tandem duplications, resulting in gene gains and losses. However, it is still unknown whether different OR gene families have experienced distinct modes of gene duplication.
Editorial Material
Multidisciplinary Sciences
Emily R. Liman
Summary: Insects use odorant receptor proteins to form membrane ion channels that open when binding to an odorant molecule, allowing them to detect and distinguish between smells.
Article
Entomology
Md Zeeshan Ali, Anwar L. Bilgrami, Jawaid Ahsan
Summary: This review presents the latest research progress on olfactory and gustatory signaling in the fruit fly Drosophila melanogaster, including the molecular mechanisms involved, the receptors and signaling pathways, and the potential applications in pest and insect vector control.
Article
Multidisciplinary Sciences
Dania Jundi, Jean-Pierre Coutanceau, Erika Bullier, Soumaiya Imarraine, Ziad Fajloun, Elim Hong
Summary: The expression of olfactory receptor (OR) genes in non-olfactory tissues has been found in rodents and humans, with over 400 genes in humans and 1000 in rodents. Zebrafish have around 140 OR genes, with 31 genes expressed in various tissues, showing conservation of expression in non-olfactory tissues across vertebrates.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Dania Jundi, Jean-Pierre Coutanceau, Erika Bullier, Soumaiya Imarraine, Ziad Fajloun, Elim Hong
Summary: This study identified the expression of 31 out of 36 olfactory receptor (OR) genes in various tissues in zebrafish larvae, including trunk, pharynx, pancreas, and brain; furthermore, some OR genes exhibited dynamic temporal expression patterns in specific brain regions across different developmental stages of zebrafish, such as the hypothalamus and the habenula.
SCIENTIFIC REPORTS
(2023)
Article
Entomology
Xiaolong Liu, Na Tong, Zheran Wu, Yang Li, Meiqi Ma, Pei Liu, Min Lu
Summary: In this study, a transcriptome analysis of Plagiodera versicolora was conducted, identifying a variety of chemosensory genes. Some of these genes showed higher expression in female antennae, potentially participating in female-specific behaviors. This research is of great importance for understanding the olfactory system and chemoreception mechanism in P. versicolora.
Article
Biochemistry & Molecular Biology
Sainan Zhang, Meng Li, Yabei Xu, Yuxuan Zhao, Yiming Niu, Shixiang Zong, Jing Tao
Summary: This study identified 23 AglaORs genes associated with the detection of pheromones, including 11 male-biased and 2 female-biased genes, which were more significantly expressed in the sexual maturation stage. Additionally, 2 female-biased AglaORs genes were overexpressed in females seeking spawning grounds after mating, suggesting their involvement in the recognition of host plant volatiles for regulating spawning ground selection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ria Gupta, Aayushi Mittal, Vishesh Agrawal, Sushant Gupta, Krishan Gupta, Rishi Raj Jain, Prakriti Garg, Sanjay Kumar Mohanty, Riya Sogani, Harshit Singh Chhabra, Vishakha Gautam, Tripti Mishra, Debarka Sengupta, Gaurav Ahuja
Summary: The molecular mechanisms of olfaction are less explored compared to other sensory systems. OdoriFy is a web server that offers various functions, such as odorant identification, chemical classification, and responsive receptor identification. Utilizing explainable artificial intelligence, OdoriFy provides the rationale behind each prediction.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Wai Yee Low, Benjamin D. Rosen, Yan Ren, Derek M. Bickhart, Thu-Hien To, Fergal J. Martin, Konstantinos Billis, Tad S. Sonstegard, Shawn T. Sullivan, Stefan Hiendleder, John L. Williams, Michael P. Heaton, Timothy P. L. Smith
Summary: The study reported the first gaur reference genome and identified three biological pathways with significant changes in gene copy numbers, reflecting potential adaptation to environmental challenges. Comparative analysis with other domesticated cattle revealed genomic signatures of artificial selection, providing insights into selection for male fertility.
Article
Biochemistry & Molecular Biology
Adnan S. Syed, Kanika Sharma, Maxime Policarpo, Sara Ferrando, Didier Casane, Sigrun Korsching
Summary: Cartilaginous fishes have a low and stable number of olfactory chemosensory receptors belonging to the OR, TAAR, and V1R/ORA families, while the number of V2R/OlfC receptors is higher and more dynamic. The V2R/OlfC receptors are expressed in the olfactory epithelium in a sparse pattern, indicating the same cell-type specificity as bony fishes. The relatively low number of olfactory receptors in cartilaginous fishes compared to bony fishes may be due to a trade-off between olfactory sensitivity and discrimination capability.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Article
Plant Sciences
Hai-Xia Zhan, Lan Li, Feng-Qi Li, Lian-Sheng Zang
Summary: In this study, the antennal and abdominal transcriptomes of Anastatus japonicus Ashmead were sequenced and analyzed to understand the olfactory mechanisms in this species. A total of 201 putative olfactory receptor genes, including 184 odorant receptors (ORs) and 17 ionotropic receptors (IRs), were identified. Tissue-specific and sex-biased expression profiles of these genes were assayed, revealing significant differences in expression levels between female and male antennae. These findings provide valuable insights into the molecular mechanisms of the olfactory system in A. japonicus.
Article
Biotechnology & Applied Microbiology
Francisca Rojas Ringeling, Shounak Chakraborty, Caroline Vissers, Derek Reiman, Akshay M. Patel, Ki-Heon Lee, Ari Hong, Chan-Woo Park, Tim Reska, Julien Gagneur, Hyeshik Chang, Maria L. Spletter, Ki-Jun Yoon, Guo-li Ming, Hongjun Song, Stefan Canzar
Summary: Ladder-seq is a method that improves the quality of RNA sequencing transcriptomes by separating transcripts by length before sequencing. Simulated data showed that Ladder-seq accurately quantifies transcripts of complex genes and has higher accuracy and sensitivity in transcript assembly. In experimental data, Ladder-seq identifies more genes with isoform switches and reveals widespread changes in isoform usage after m(6)A depletion.
NATURE BIOTECHNOLOGY
(2022)
Article
Neurosciences
Qijing Xie, Jiefu Li, Hongjie Li, Namrata D. Udeshi, Tanya Svinkina, Daniel Orlin, Sayeh Kohani, Ricardo Guajardo, D. R. Mani, Chuanyun Xu, Tongchao Li, Shuo Han, Wei Wei, S. Andrew Shuster, David J. Luginbuhl, Stephen R. Quake, Swetha E. Murthy, Alice Y. Ting, Steven A. Carr, Liqun Luo
Summary: The transcription factor Acj6 regulates the precise dendrite targeting of Drosophila olfactory projection neurons by controlling the expression of cell-surface proteins. This study identifies specific cell-surface proteins that execute Acj6-regulated wiring decisions and demonstrates that Acj6 employs unique sets of these proteins in different neuron types for dendrite targeting. The combined expression of these proteins effectively rescues mutant phenotypes, highlighting the importance of Acj6 in controlling wiring specificity.
Article
Multidisciplinary Sciences
Hongjie Li, Jasper Janssens, Maxime De Waegeneer, Sai Saroja Kolluru, Kristofer Davie, Vincent Gardeux, Wouter Saelens, Fabrice David, Maria Brbic, Jure Leskovec, Colleen N. McLaughlin, Qijing Xie, Robert C. Jones, Katja Brueckner, Jiwon Shim, Sudhir Gopal Tattikota, Frank Schnorrer, Katja Rust, Todd G. Nystul, Zita Carvalho-Santos, Carlos Ribeiro, Soumitra Pal, Sharvani Mahadevaraju, Teresa M. Przytycka, Aaron M. Allen, Stephen F. Goodwin, Cameron W. Berry, Margaret T. Fuller, Helen White-Cooper, Erika L. Matunis, Stephen DiNardo, Anthony Galenza, Lucy Erin O'Brien, Julian A. T. Dow, Heinrich Jasper, Brian Oliver, Norbert Perrimon, Bart Deplancke, Stephen R. Quake, Liqun Luo, Stein Aerts
Summary: This study presents a single-cell atlas of the fruit fly Drosophila melanogaster, including 580,000 nuclei and annotations of over 250 distinct cell types. It serves as a valuable resource for the Drosophila community and provides a reference for studying genetic perturbations and disease models at single-cell resolution.
Article
Multidisciplinary Sciences
Jessica A. Osterhout, Vikrant Kapoor, Stephen W. Eichhorn, Eric Vaughn, Jeffrey D. Moore, Ding Liu, Dean Lee, Laura A. DeNardo, Liqun Luo, Xiaowei Zhuang, Catherine Dulac
Summary: This study identifies a previously uncharacterized population of neurons in the brain that are activated after infection, playing a crucial role in generating fever response and other sickness symptoms. By examining gene expression and electrophysiological measurements, the researchers uncover a mechanism where these neurons are influenced by immune signals released by non-neuronal cells during infection and are functionally connected to brain areas controlling body temperature and appetite.
Editorial Material
Neurosciences
Jalal Baruni, Liqun Luo
Summary: In this study, Paquelet et al. utilize microendoscopy to record the activity of over 2,000 dorsal raphe serotonin neurons, providing new insights into their function at both the level of individual neurons and the population.
Article
Multidisciplinary Sciences
Jun Xu, Yifang Liu, Hongjie Li, Alexander J. Tarashansky, Colin H. Kalicki, Ruei-Jiun Hung, Yanhui Hu, Aram Comjean, Sai Saroja Kolluru, Bo Wang, Stephen R. Quake, Liqun Luo, Andrew P. McMahon, Julian A. T. Dow, Norbert Perrimon
Summary: The single-nucleus study of the Drosophila renal system identified various cell types and their roles in kidney function and regeneration, providing insights for the generation of disease models.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Editorial Material
Biochemistry & Molecular Biology
Colleen N. McLaughlin, Liqun Luo
Summary: In this article, the authors reveal that mosquitoes' strong attraction to humans may be attributed to non-canonical olfactory circuit organization and coding, which enables them to locate human hosts effectively.
Article
Neurosciences
S. Andrew Shuster, Jiefu Li, URee Chon, Miley C. Sinantha-Hu, David J. Luginbuhl, Namrata D. Udeshi, Dominique Kiki Carey, Yukari H. Takeo, Qijing Xie, Chuanyun Xu, D. R. Mani, Shuo Han, Alice Y. Ting, Steven A. Carr, Liqun Luo
Summary: A new method called iPEEL was developed to identify cell-surface proteomes in specific cell types in their native tissues. By applying iPEEL to studying developing and mature cerebellar Purkinje cells, researchers discovered differential enrichment in cell-surface proteomes with post-translational protein processing and synaptic functions in these cells. They also identified the critical role of Armh4 in Purkinje cell dendrite morphogenesis through in vivo loss-of-function screening. Disrupting Armh4's endocytosis augmented its effect on dendrite morphogenesis. This study highlights the importance of CSP profiling in native mammalian tissues for understanding cell-surface signaling regulations.
Article
Multidisciplinary Sciences
Ya-Ting Chang, Max Kowalczyk, P. Michelle Fogerson, Yu-Ju Lee, Minza Haque, Eliza L. Adams, David C. Wang, Laura A. DeNardo, Marc Tessier-Lavigne, John R. Huguenard, Liqun Luo, Wei-Hsiang Huang
Summary: Hyperexcitability of brain circuits is a common feature of autism spectrum disorders. This study identifies Rai1 as a negative regulator of excitability, and its deletion results in increased seizure susceptibility and prolonged hippocampal seizure duration.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Taehong Yang, Daniel W. Bayless, Yichao Wei, Dan Landayan, Ivo M. Marcelo, Yangpeng Wang, Laura A. DeNardo, Liqun Luo, Shaul Druckmann, Nirao M. Shah
Summary: Social interactions rely on the awareness and understanding of others' behavior. Mirror neurons, which represent actions of self and others, are crucial for this cognitive process. In this study, we identified mirror neurons in the hypothalamus of mice and found that their activity is essential for aggression and can even trigger aggressive displays towards mirror images.
Article
Biochemical Research Methods
Yuhang Fan, Zaneta Andrusivova, Yunming Wu, Chew Chai, Ludvig Larsson, Mengxiao He, Liqun Luo, Joakim Lundeberg, Bo Wang
Summary: Expansion spatial transcriptomics (Ex-ST) combines tissue expansion and an improved RNA capture protocol to achieve high spatial resolution in array-based spatial transcriptomics. By clearing and expanding tissue before capturing the entire polyadenylated transcriptome, Ex-ST overcomes the limitation of array density. We demonstrate its effectiveness using mouse brain samples.
Article
Multidisciplinary Sciences
Tzu-Chiao Lu, Maria Brbic, Ye-Jin Park, Tyler Jackson, Jiaye Chen, Sai Saroja Kolluru, Yanyan Qi, Nadja Sandra Katheder, Xiaoyu Tracy Cai, Seungjae Lee, Yen -Chung Chen, Niccole Auld, Chung-Yi Liang, Sophia H. Ding, Doug Welsch, Samuel D'Souza, Angela Oliveira Pisco, Robert C. Jones, Jure Leskovec, Eric C. Lai, Hugo J. Bellen, Liqun Luo, Heinrich Jasper, Stephen R. Quake, Hongjie Li
Summary: This study presents the Aging Fly Cell Atlas, which is a single-nucleus transcriptomic map of the whole aging Drosophila. It reveals changes in tissue cell composition, gene expression, and cell identities during aging, and develops aging clock models that predict fly age based on ribosomal gene expression. The atlas provides a valuable resource for studying fundamental principles of aging in complex organisms.
Article
Biochemistry & Molecular Biology
Daniel W. Bayless, Chung-ha O. Davis, Renzhi Yang, Yichao Wei, Vinicius Miessler de Andrade Carvalho, Joseph R. Knoedler, Taehong Yang, Oscar Livingston, Akira Lomvardas, Gabriela J. Martins, Ana Mafalda Vicente, Jun B. Ding, Liqun Luo, Nirao M. Shash
Summary: This study has identified a neural circuit that governs key aspects of innate male sexual behavior, including motor displays, drive, and reward. This circuit connects chemosensory input to neurons that regulate motor output and reward centers.
Article
Biochemical Research Methods
Luka Borozan, Francisca Rojas Ringeling, Shao-Yen Kao, Elena Nikonova, Pablo Monteagudo-Mesas, Domagoj Matijevic, Maria L. Spletter, Stefan Canzar
Summary: In this study, a method called fortuna is proposed, which creates transcript fragments by guessing novel combinations of annotated splice sites and pseudoaligns reads to fragments using kallisto. The method is around 7x faster than traditional align and count approaches, accurately maps reads containing mismatches across novel junctions, and identifies more reads supporting aberrant splicing events in patients with autism spectrum disorder.
Article
Multidisciplinary Sciences
Ya-Hui Chou, Chi-Jen Yang, Hao-Wei Huang, Nan-Fu Liou, Michael Raphael Panganiban, David Luginbuhl, Yijie Yin, Istvan Taisz, Liang Liang, Gregory S. X. E. Jefferis, Liqun Luo
Summary: In this study, the authors used genetic labeling and electron microscopy connectomics to investigate the variability of a specific neuron, TC-LN, in its innervation patterns. They found that this neuron exhibits extraordinary variability and that it is influenced by factors such as sex, courtship experience, and mating in relation to food intake. The researchers propose that this mating-associated variability regulates how food odor is interpreted by the inhibitory network to modulate feeding behavior.