Article
Biochemistry & Molecular Biology
Richard Huang, Jalna Meens, Scott Yuzwa, Laurie Ailles, Michael Ohh, Claire M. Robinson
Summary: Cancer stem cells play an important role in tumor biology. This study aimed to identify the existence of clear cell renal cell carcinoma (ccRCC) stem cells using the side population approach, but the results did not support the presence of cancer stem cells in ccRCC.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Qing Guo, Shuai Shen, Gefei Guan, Chen Zhu, Cunyi Zou, Jingyuan Cao, Wen Cheng, Xiaoyan Xu, Juanhan Yu, Zhiguo Lin, Guoli Wang, Ling Chen, Peng Cheng, Anhua Wu
Summary: Glioblastoma (GBM) shares common signaling pathways involving TIM-3, an immune checkpoint, between tumor and non-tumor cells. The study reveals that TIM-3 in glioma cells not only regulates the malignant behaviors of glioma cells but also induces macrophage migration and transition to an anti-inflammatory/protumorigenic phenotype through the TIM-3/IL6 signal. Blocking this feedback loop may provide a novel therapeutic strategy for GBM.
Article
Immunology
Peiwen Chen
Summary: TFPI2 has been identified as a crucial molecule in the symbiotic interaction between glioblastoma stem cells and microglia. Blocking this symbiosis not only inhibits tumor growth but also synergizes with an immune checkpoint inhibitor in mouse models of glioblastoma.
Article
Chemistry, Physical
Ashok Kumar Dhinakaran, Swarna Ganesh, Rupa Haldavnekar, Bo Tan, Sunit Das, Krishnan Venkatakrishnan
Summary: Liquid biopsy has the potential to serve as a clinically viable diagnostic tool for glioblastoma, as it can detect circulating glioblastoma stem cell-associated cell free DNA accurately and rapidly with high diagnostic accuracy. The use of nanoengineered plasmonic metasensors allows for real-time genomic profiling of the DNA, enabling the classification of GBM with high specificity and accuracy.
Article
Cell Biology
Fangfang Wang, Zhang Dan, Hongmei Luo, Jingcao Huang, Yushan Cui, Hong Ding, Juan Xu, Zhimei Lin, Yuhan Gao, Xinyu Zhai, Yan Yang, Ying Qu, Li Zhang, Fengjiao Chen, Qiang Wang, Xin Wang, Yu Feng, Ting Liu, Qing Yi, Ting Niu, Yuhuan Zheng
Summary: Drug-resistance is a major problem in multiple myeloma (MM) patients. This study reveals that activated-leukocyte-cell-adhesion-molecule (ALCAM) regulates MM side population (SP)-mediated drug-resistance through the ALCAM-EGF/EGFR axis. EGFR activation promotes the SP ratio, while ALCAM inhibits EGFR downstream signaling in MM cells. SP MM cells have a higher number of mitochondria and interference of mitochondrial function inhibits SP-genesis. Combination therapy with an anti-MM agent and EGFR inhibitor gefitinib improves MM therapeutic efficacy and prolongs survival in MM-bearing mice.
CELL DEATH & DISEASE
(2022)
Article
Engineering, Biomedical
Wentao Wang, Fan Wu, Mohsen Mohammadniaei, Ming Zhang, Yuanyuan Li, Yi Sun, Ben Zhong Tang
Summary: Researchers have developed T-cell-mimic nanoparticles with aggregation-induced emission characteristics to target glioblastoma cells and glioblastoma stem cells. These nanoparticles can cross the blood-brain barrier and effectively inhibit tumor growth and recurrence through photothermal therapy.
Article
Multidisciplinary Sciences
Xiaoli Dong, Yingying Wei, Tao Xu, Xiaoyue Tan, Na Li
Summary: Cancer stem cells (CSCs) play a crucial role in tumor growth, metastasis, and recurrence. Techniques such as staining tumor cells with Hoechst 33342 and analyzing the proportion of side population (SP) cells using flow cytometry provide convenient, fast, and cost-effective methods for the identification and purification of CSCs. Data generated from these assays can contribute to a better understanding of the stemness properties of tumor cells in response to various signals.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Immunology
Saif Khan, Mohd Wajid Ali Khan, Subuhi Sherwani, Sultan Alouffi, Mohammad Jahoor Alam, Khalid Al-Motair, Shahper Khan
Summary: A probabilistic model based on binding scores and differential expression levels of cancer cell membrane proteins was used to predict the selective targeting efficiency of cancer cell membrane coated nanoparticles. The model was experimentally validated and showed high accuracy. This model has the potential to aid in the development of more specific cancer immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Carolin Seifert, Ellen Balz, Susann Herzog, Anna Korolev, Sebastian Gassmann, Heiko Paland, Matthias A. Fink, Markus Grube, Sascha Marx, Gabriele Jedlitschky, Mladen Tzvetkov, Bernhard H. Rauch, Henry W. S. Schroeder, Sandra Bien-Moeller
Summary: Research has shown that PIM1 kinase plays a significant role in the behavior of glioblastoma stem cells, and its inhibition can lead to the death of these stem-like cells, suggesting a potential approach for glioblastoma therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Yan-jie Weng, Xiao-xiao Zhang, Xue Wu, Li-li Guo, Chang-yu Wang
Summary: The study found that different subtypes of ovarian cancer SP cells have varying levels of stemness, with the LSP cell subtype exhibiting higher stem cell marker expression, greater in vitro sphere-forming capability, increased cisplatin resistance, and enhanced in vivo tumorigenicity compared to USP cells. NSP cells showed no stemness characteristics.
CURRENT MEDICAL SCIENCE
(2021)
Article
Oncology
Cecile Doualle, Julien Gouju, Yousra Nouari, Meline Wery, Clelia Guittonneau, Philippe Codron, Audrey Rousseau, Patrick Saulnier, Joel Eyer, Franck Letournel
Summary: In this study, four GSC models obtained through dedifferentiation were extensively characterized and compared to two established patient-derived GSC lines. The dedifferentiated GSC models showed similar characteristics and marker expressions to the patient-derived GSC lines, confirming their relevance for future GSC targeting therapies and emphasizing the potential of the CXCR4 pathway as a therapeutic target.
AMERICAN JOURNAL OF CANCER RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Wenqian Geng, Hao Zou, Hongbo Wang, Yu Dai, Guangzhao Lu, Zhiguo Sun, Ying Lu, Xueying Ding, Yuan Yu
Summary: The biomimetic vehicle based on low density lipoprotein showed significant inhibition of GSC microsphere formation and induced apoptosis in GSCs. It also reduced the formation of GSC-derived endothelial tubules, inhibited endothelial cell migration and angiogenesis, and improved drug transport into the brain. The designed endogenous biomimetic nanomedicine has the potential to enhance intracranial tumor treatments and reduce neurotoxicity.
Article
Oncology
Changlin Yang, Guimei Tian, Mariana Dajac, Andria Doty, Shu Wang, Ji-Hyun Lee, Maryam Rahman, Jianping Huang, Brent A. Reynolds, Matthew R. Sarkisian, Duane Mitchell, Loic P. Deleyrolle
Summary: This study reveals the diversity of cell types and dynamic behaviors in glioblastoma, particularly the heterogeneity between slow-cycling cells and cells defined by the expression of stem cell markers. The findings shed light on the potential mechanisms underlying tumor resilience and treatment resistance, and provide insights for the development of precision and effective therapies for brain cancer.
Review
Immunology
Guangwen Wang, Wenshi Wang
Summary: GBM is a highly treatment-resistant tumor with a poor prognosis, and it has proven to be resistant to both traditional and newer immunotherapies. The need for innovative and efficient treatment strategies is urgent. This review provides a comprehensive overview of various cell therapies currently being used to treat GBM, discussing their efficacy, safety, mechanisms, and challenges, with the aim of identifying the next-generation immunotherapeutic approach to eliminate this dreadful disease.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Paul Guilhamon, Charles Chesnelong, Michelle M. Kushida, Ana Nikolic, Divya Singhal, Graham MacLeod, Seyed Ali Madani Tonekaboni, Florence M. G. Cavalli, Christopher Arlidge, Nishani Rajakulendran, Naghmeh Rastegar, Xiaoguang Hao, Rozina Hassam, Laura J. Smith, Heather Whetstone, Fiona J. Coutinho, Bettina Nadorp, Katrina Ellestad, H. Artee Luchman, Jennifer Ai-wen Chan, Molly S. Shoichet, Michael D. Taylor, Benjamin Haibe-Kains, Samuel Weiss, Stephane Angers, Marco Gallo, Peter B. Dirks, Mathieu Lupien
Summary: This study uses chromatin accessibility to identify diverse self-renewing populations in glioblastoma, with three distinct states identified in glioblastoma stem cells. The characterization of these states improves prognostic precision and guides combination therapies for better treatment outcomes.
Letter
Radiology, Nuclear Medicine & Medical Imaging
Kazunobu Ohnuki, Mitsuyoshi Yoshimoto, Hirofumi Fujii
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2021)
Article
Materials Science, Biomaterials
Thi Kim Dung Doan, Masakazu Umezawa, Kazunobu Ohnuki, Karina Nigoghossian, Kyohei Okubo, Masao Kamimura, Masayuki Yamaguchi, Hirofumi Fujii, Kohei Soga
Summary: In this study, a new bimodal OTN-NIR/MR imaging probe was designed using an OTN-NIR fluorescent organic dye and Gd-DOTA, providing high sensitivity and structural information. The probe exhibited good OTN-NIR fluorescence and MRI contrast enhancement in mouse imaging experiments. The results demonstrated the importance of the Gd-DOTA introduction ratio in controlling the biological response.
BIOMATERIALS SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Yukina Morimoto, Ayako Tokumitsu, Takefumi Sone, Yuki Hirota, Ryota Tamura, Ayuna Sakamoto, Kazunori Nakajima, Masahiro Toda, Yutaka Kawakami, Hideyuki Okano, Shigeki Ohta
Summary: This study aimed to identify factors that promote the expansion of Neural Stem Cell/Progenitor Cells (NSPCs) and understand their molecular mechanisms in cell proliferation and survival. Translationally-controlled tumor protein-1 (TPT1) was identified as a downstream target of Macrophage Migration Inhibitory Factor (MIF) signaling, involved in the regulation of proliferation and survival in NSPCs and brain tumor-initiating cells (BTICs).
NEUROCHEMICAL RESEARCH
(2022)
Article
Oncology
Hidetaka Suzuki, Shuichi Mitsunaga, Masafumi Ikeda, Takao Aoyama, Kazumi Yoshizawa, Masayuki Yamaguchi, Masami Suzuki, Minoru Narita, Toshikatsu Kawasaki, Atsushi Ochiai
Summary: This study revealed that the IL-6/gp130 axis promotes nerve invasion in PDAC and is upregulated in PDAC patients with severe nerve invasion. This offers a potential target for therapeutic intervention in PDAC with nerve invasion.
Article
Engineering, Biomedical
Atsushi Yagishita, Shin'ichiro Takeda, Miho Katsuragawa, Tenyo Kawamura, Hideaki Matsumura, Tadashi Orita, Izumi O. Umeda, Goro Yabu, Pietro Caradonna, Tadayuki Takahashi, Shin Watanabe, Yousuke Kanayama, Hiroshi Mizuma, Kazunobu Ohnuki, Hirofumi Fujii
Summary: This study reports the development and performance of an imaging system equipped with a cadmium telluride diode detector that achieves high energy and spatial resolutions. By fitting high-resolution spectra to an X-ray analysis model, multiple radionuclides can be simultaneously visualized in living tissue.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Oncology
Makoto Nakagawa, Masayuki Yamaguchi, Makoto Endo, Yukino Machida, Ayuna Hattori, Fumie Tanzawa, Shinji Tsutsumi, Issay Kitabayashi, Akira Kawai, Fumihiko Nakatani
Summary: The study suggests that levels of 2-HG in both tumor tissue and serum can serve as potential biomarkers for IDH-mutant tumors, and the 2-HG signal in MR spectra has the potential to be a non-invasive biomarker.
JOURNAL OF BONE ONCOLOGY
(2022)
Letter
Radiology, Nuclear Medicine & Medical Imaging
Kazunobu Ohnuki, Yasumasa Takenaka, Mitsuyoshi Yoshimoto, Hirofumi Fujii
ANNALS OF NUCLEAR MEDICINE
(2022)
Article
Oncology
Yuki Katoh, Tomonori Yaguchi, Akiko Kubo, Takashi Iwata, Kenji Morii, Daiki Kato, Shigeki Ohta, Ryosuke Satomi, Yasuhiro Yamamoto, Yoshitaka Oyamada, Kota Ouchi, Shin Takahashi, Chikashi Ishioka, Ryo Matoba, Makoto Suematsu, Yutaka Kawakami
Summary: This study investigated the immunological roles of SCD1 in cancer immune response, finding that SCD1 inhibition augments antitumor T cells and enhances the therapeutic effects of anti-PD-1 antibody. High SCD1 expression was observed in a non-T cell-inflamed subtype of human colon cancer, and serum SCD1 related fatty acids were correlated with response rates and prognosis of non-small lung cancer patients following anti-PD-1 antibody treatment. Therefore, SCD1 is a promising target for improving cancer immunotherapies.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Kazunobu Ohnuki, Mitsuyoshi Yoshimoto, Hiromitsu Haba, Shino Manabe, Hiroki Takashima, Masahiro Yasunaga, Yasumasa Takenaka, Hirofumi Fujii
Summary: This study investigated the permeability of four types of plastic films and two types of rubber gloves to At-211 and identified suitable materials for preventing contamination. The results showed that polyvinylidene chloride, a laminated film, and nitrile rubber provided better protection against At-211 contamination compared to polyethylene, polyvinyl chloride, and latex rubber.
Review
Dermatology
Shigeki Ohta, Aya Misawa, Chaw Kyi-Tha-Thu, Naomi Matsumoto, Yoshie Hirose, Yutaka Kawakami
Summary: Melanoma has been extensively studied as a model for cancer immunology research, and investigations into anti-tumor T-cell responses have provided valuable insights for immunotherapies. Individual differences in cancer-immune status are influenced by cancer cell characteristics, genetic backgrounds, and environmental and lifestyle factors. Recent studies using advanced technologies have led to advancements in human tumor immunology and the development of personalized immunotherapies for melanoma patients.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Masayuki Yamaguchi, Kosuke Kojo, Mizuki Akatsuka, Tomoyuki Haishi, Tatsushi Kobayashi, Takahito Nakajima, Hiroyuki Nishiyama, Hirofumi Fujii
Summary: We developed a new device, consisting of a 3-cm RF coil and an immobilizer, for high-resolution MR imaging of the testis. A cohort of healthy volunteers underwent an MRI study using this device, with the immobilizer and RF coil placed on the scrotum. T2-weighted images were acquired with an in-plane resolution of 117 microns using a 3-T MR scanner and the PROPELLER sequence. The combined use of a small RF coil and an immobilizer proved to be a feasible option for obtaining high-resolution MR images of the testis.
MAGNETIC RESONANCE IN MEDICAL SCIENCES
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Keisuke Tsuda, Takayuki Suzuki, Kazuhito Toya, Eisuke Sato, Hirofumi Fujii
Summary: In this study, the optimal reconstruction algorithm in FDG PET with a short acquisition time was investigated. The images reconstructed by FORE + OSEM showed poorer image quality compared to 3D-OSEM, but they provided clearer FDG uptake and were more useful for the detection of lesions.
WORLD JOURNAL OF NUCLEAR MEDICINE
(2023)
Article
Materials Science, Biomaterials
Thi Kim Dung Doan, Masakazu Umezawa, Kyohei Okubo, Masao Kamimura, Masayuki Yamaguchi, Hirofumi Fujii, Kohei Soga
Summary: Multimodal imaging, combining over one-thousand-nanometer near-infrared (OTN-NIR) fluorescence and magnetic resonance (MR) imaging, is attractive for biomedical research. In this study, a bimodal OTN-NIR/MRI probe was developed by encapsulating IR-1061 in a gadolinium-tetraazacyclododecanetetraacetic acid (Gd-DOTA) conjugated poly(lactic-co-glycolic acid)-block-poly(ethylene glycol) copolymer (PLGA-b-PEG) micelle at two different locations. The probe structure with Gd-DOTA at the border of PLGA/PEG showed stable fluorescence and signal distribution, indicating enhanced structural stability and performance.
BIOMATERIALS SCIENCE
(2022)
Meeting Abstract
Oncology
Aya Misawa, Shigeki Ohta, Naoshi Kawamura, Hidetoshi Sumimoto, Rumi Sakaguchi, Ryotaro Imagawa, Yuki Katoh, Takahiro Tsujikawa, Kenta Nakamura, Ryuhei Okuyama, Yutaka Kawakami
Meeting Abstract
Oncology
Shigeki Ohta, Naoki Izawa, Aya Misawa, Yuki Katoh, Akiko Kubo, Shigenori Kadowaki, Keiko Minashi, Tomohiro Nishina, Takako Nakajima Eguchi, Yutaka Kawakami
Review
Oncology
Xinru Zhou, Yin Jia, Chuanbin Mao, Shanrong Liu
Summary: Small extracellular vesicles (sEVs), such as exosomes, have emerged as crucial targets for liquid biopsy and promising drug delivery vehicles in tumor progression. They can serve as biomarkers for tumor diagnosis and as drug carriers for cancer treatment.
Article
Oncology
Ruochan Chen, Ju Zhu, Xiao Zhong, Jie Li, Rui Kang, Daolin Tang
Summary: The interplay between autophagy and apoptosis plays a crucial role in tumorigenesis and cancer therapy, with HMGB1 serving as a key regulator in these processes.
Article
Oncology
Zongfu Pan, Xixuan Lu, Tong Xu, Jinming Chen, Lisha Bao, Ying Li, Yingying Gong, Yulu Che, Xiaozhou Zou, Zhuo Tan, Ping Huang, Minghua Ge
Summary: This study uncovered the emerging role of HN1 in promoting dedifferentiation of anaplastic thyroid cancer (ATC) cells. HN1 negatively regulated the thyroid differentiation markers and had an inhibitory effect on the transcriptional activation of CTCF, thereby influencing the chromatin accessibility of thyroid differentiation genes.
Article
Oncology
Yi Qin, Shengjun Xiong, Jun Ren, Gautam Sethi
Summary: Autophagy plays an important regulatory role in glioblastoma, and its dysregulation can lead to drug resistance and radioresistance. It also affects stem cell characteristics, overall growth, and metastasis. Therefore, autophagy is a promising target for glioblastoma therapy.
Article
Oncology
Katsuya Nagaoka, Xuewei Bai, Dan Liu, Kevin Cao, Joud Mulla, Chengcheng Ji, Hongze Chen, Muhammad Azhar Nisar, Amalia Bay, William Mueller, Grace Hildebrand, Jin-Song Gao, Shaolei Lu, Hiroko Setoyama, Yasuhito Tanaka, Jack R. Wands, Chiung-Kuei Huang
Summary: This study found that serum 2-OG levels in cholangiocarcinoma patients are associated with the effectiveness of chemotherapy. Patients with progressive disease showed significantly higher levels of serum 2-OG compared to stable disease and partial response patients. The study also revealed that overexpression of ASPH mimics the effects of 2-OG, and knockdown of ASPH improves chemotherapy. Targeting ASPH enhances the effects of chemotherapy by modulating ATM and ATR, two key regulators of DDRs.
