Insect omics research coming of age1This review is part of a virtual symposium on recent advances in understanding a variety of complex regulatory processes in insect physiology and endocrinology, including development, metabolism, cold hardiness, food intake and digestion, and diuresis, through the use of omics technologies in the postgenomic era.

Quantitative, High-Resolution Proteomics for Data-Driven Systems Biology

(2011) Jürgen Cox et al.   Annual Review of Biochemistry

Microarrays, deep sequencing and the true measure of the transcriptome

(2011) John H Malone et al.   BMC BIOLOGY

A honey bee (Apis mellifera L.) PeptideAtlas crossing castes and tissues

(2011) Queenie WT Chan et al.   BMC GENOMICS

Ras1 CA overexpression in the posterior silk gland improves silk yield

(2011) Li Ma et al.   CELL RESEARCH

Locust phase polyphenism: Does epigenetic precede endocrine regulation?

(2011) Bart Boerjan et al.   GENERAL AND COMPARATIVE ENDOCRINOLOGY

The genome of the leaf-cutting antAcromyrmex echinatiorsuggests key adaptations to advanced social life and fungus farming

(2011) Sanne Nygaard et al.   GENOME RESEARCH

Pyrosequencing-based expression profiling and identification of differentially regulated genes from Manduca sexta, a lepidopteran model insect

(2011) Shuguang Zhang et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Fruitless RNAi knockdown in males interferes with copulation success in Schistocerca gregaria

(2011) Bart Boerjan et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

The circadian clock genes affect reproductive capacity in the desert locust Schistocerca gregaria

(2011) Julie Tobback et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Novel Royal Jelly Proteins Identified by Gel-Based and Gel-free Proteomics

(2011) Bin Han et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Role of the Halloween genes, Spook and Phantom in ecdysteroidogenesis in the desert locust, Schistocerca gregaria

(2011) Elisabeth Marchal et al.   JOURNAL OF INSECT PHYSIOLOGY

Mitochondrial Proteins Differential Expression during Honeybee (Apis melliferaL.) Queen and Worker Larvae Caste Determination

(2011) Desalegn Begna et al.   JOURNAL OF PROTEOME RESEARCH

Predicted Effector Molecules in the Salivary Secretome of the Pea Aphid (Acyrthosiphon pisum): A Dual Transcriptomic/Proteomic Approach

(2011) James C. Carolan et al.   JOURNAL OF PROTEOME RESEARCH

Quand Tribolium complémente la génétique de la drosophile

(2011) François Bonneton   M S-MEDECINE SCIENCES

Large-Scale Label-Free Quantitative Proteomics of the Pea aphid-BuchneraSymbiosis

(2011) Anton Poliakov et al.   MOLECULAR & CELLULAR PROTEOMICS

Genome-wide analysis of alternative reproductive phenotypes in honeybee workers

(2011) DRIES CARDOEN et al.   MOLECULAR ECOLOGY

Next-generation transcriptome assembly

(2011) Jeffrey A. Martin et al.   NATURE REVIEWS GENETICS

In search for non-steroidogenic functions of the prothoracic glands of the desert locust, Schistocerca gregaria: A peptidomic and proteomic approach

(2011) Bart Boerjan et al.   PEPTIDES

Transcriptome Analysis of the Desert Locust Central Nervous System: Production and Annotation of a Schistocerca gregaria EST Database

(2011) Liesbeth Badisco et al.   PLoS One

Differential Proteomics in Dequeened Honeybee Colonies Reveals Lower Viral Load in Hemolymph of Fertile Worker Bees

(2011) Dries Cardoen et al.   PLoS One

Knockdown of Midgut Genes by dsRNA-Transgenic Plant-Mediated RNA Interference in the Hemipteran Insect Nilaparvata lugens

(2011) Wenjun Zha et al.   PLoS One

The Worker Honeybee Fat Body Proteome Is Extensively Remodeled Preceding a Major Life-History Transition

(2011) Queenie W. T. Chan et al.   PLoS One

Microarray-Based Transcriptomic Analysis of Differences between Long-Term Gregarious and Solitarious Desert Locusts

(2011) Liesbeth Badisco et al.   PLoS One

Knickkopf protein protects and organizes chitin in the newly synthesized insect exoskeleton

(2011) S. S. Chaudhari et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The genome of the fire ant Solenopsis invicta

(2011) Y. Wurm et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Draft genome of the red harvester ant Pogonomyrmex barbatus

(2011) C. R. Smith et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Creating a Buzz About Insect Genomes

(2011) G. E. Robinson et al.   SCIENCE

The Genome Sequence of the Leaf-Cutter Ant Atta cephalotes Reveals Insights into Its Obligate Symbiotic Lifestyle

(2011) Garret Suen et al.   PLoS Genetics

Knockdown of ecdysis-triggering hormone gene with a binary UAS/GAL4 RNA interference system leads to lethal ecdysis deficiency in silkworm

(2010) H. Dai et al.   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

Oxytocin/vasopressin and gonadotropin-releasing hormone from cephalopods to vertebrates

(2010) Hiroyuki Minakata   Annals of the New York Academy of Sciences

Increase of Drosophila melanogaster lifespan due to D-GADD45 overexpression in the nervous system

(2010) E. N. Plyusnina et al.   BIOGERONTOLOGY

Reverse genetics in eukaryotes

(2010) Serge Hardy et al.   BIOLOGY OF THE CELL

Functionality of the GAL4/UAS system in Tribolium requires the use of endogenous core promoters

(2010) Johannes B Schinko et al.   BMC DEVELOPMENTAL BIOLOGY

The past, present and future of gene targeting in Drosophila

(2010) Natalia Wesolowska et al.   FLY

Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection

(2010) Yoko Takasu et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Neuropeptide and neurohormone precursors in the pea aphid, Acyrthosiphon pisum

(2010) J. Huybrechts et al.   INSECT MOLECULAR BIOLOGY

A functional DNA methylation system in the pea aphid, Acyrthosiphon pisum

(2010) T. K. Walsh et al.   INSECT MOLECULAR BIOLOGY

Feeding-based RNA interference of atrehalose phosphate synthasegene in the brown planthopper,Nilaparvata lugens

(2010) J. Chen et al.   INSECT MOLECULAR BIOLOGY

Chitosan/double-stranded RNA nanoparticle-mediated RNA interference to silence chitin synthase genes through larval feeding in the African malaria mosquito (Anopheles gambiae)

(2010) X. Zhang et al.   INSECT MOLECULAR BIOLOGY

RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design

(2010) Olle Terenius et al.   JOURNAL OF INSECT PHYSIOLOGY

Tangible benefits of the aphid Acyrthosiphon pisum genome sequencing for aphid proteomics: Enhancements in protein identification and data validation for homology-based proteomics

(2010) M. Cilia et al.   JOURNAL OF INSECT PHYSIOLOGY

Genomics and Peptidomics of Neuropeptides and Protein Hormones Present in the Parasitic WaspNasonia vitripennis∥

(2010) Frank Hauser et al.   JOURNAL OF PROTEOME RESEARCH

When peptides fly: Advances in Drosophila proteomics

(2010) Alexey Veraksa   Journal of Proteomics

Genetics Coupled to Quantitative Intact Proteomics Links Heritable Aphid and Endosymbiont Protein Expression to Circulative Polerovirus Transmission

(2010) M. Cilia et al.   JOURNAL OF VIROLOGY

The SILAC Fly Allows for Accurate Protein Quantificationin Vivo

(2010) Matthias D. Sury et al.   MOLECULAR & CELLULAR PROTEOMICS

Lack of evidence for DNA methylation of Invader4 retroelements in Drosophila and implications for Dnmt2-mediated epigenetic regulation

(2010) Matthias Schaefer et al.   NATURE GENETICS

RNA interference of insulin-related peptide and neuroparsins affects vitellogenesis in the desert locust Schistocerca gregaria

(2010) Liesbeth Badisco et al.   PEPTIDES

Ingested RNA interference for managing the populations of the Colorado potato beetle, Leptinotarsa decemlineata

(2010) Fang Zhu et al.   PEST MANAGEMENT SCIENCE

Differential Gene Expression and Protein Abundance Evince Ontogenetic Bias toward Castes in a Primitively Eusocial Wasp

(2010) James H. Hunt et al.   PLoS One

Differential Protein Expression in Honeybee (Apis mellifera L.) Larvae: Underlying Caste Differentiation

(2010) Jianke Li et al.   PLoS One

De Novo Transcriptome Sequencing in Anopheles funestus Using Illumina RNA-Seq Technology

(2010) Jacob E. Crawford et al.   PLoS One

Genome sequences of the human body louse and its primary endosymbiont provide insights into the permanent parasitic lifestyle

(2010) E. F. Kirkness et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Sequencing of Culex quinquefasciatus Establishes a Platform for Mosquito Comparative Genomics

(2010) P. Arensburger et al.   SCIENCE

Genomic Comparison of the Ants Camponotus floridanus and Harpegnathos saltator

(2010) R. Bonasio et al.   SCIENCE

Functional and Evolutionary Insights from the Genomes of Three Parasitoid Nasonia Species

(2010) J. H. Werren et al.   SCIENCE

Cotton plants expressing CYP6AE14 double-stranded RNA show enhanced resistance to bollworms

(2010) Ying-Bo Mao et al.   TRANSGENIC RESEARCH

The Honey Bee Epigenomes: Differential Methylation of Brain DNA in Queens and Workers

(2010) Frank Lyko et al.   PLOS BIOLOGY

Genome Sequence of the Pea Aphid Acyrthosiphon pisum

(2010)   PLOS BIOLOGY

Large-Scale Field Application of RNAi Technology Reducing Israeli Acute Paralysis Virus Disease in Honey Bees (Apis mellifera, Hymenoptera: Apidae)

(2010) Wayne Hunter et al.   PLoS Pathogens

Major Genomic Events and Their Consequences for Vertebrate Evolution and Endocrinology

(2009) Dan Larhammar et al.   Annals of the New York Academy of Sciences

Beyond Drosophila: RNAi In Vivo and Functional Genomics in Insects

(2009) Xavier Bellés   Annual Review of Entomology

Large-scale insertional mutagenesis of a coleopteran stored grain pest, the red flour beetle Tribolium castaneum, identifies embryonic lethal mutations and enhancer traps

(2009) Jochen Trauner et al.   BMC BIOLOGY

Quantitative proteomics: a tool to assess cell differentiation

(2009) Michiel Vermeulen et al.   CURRENT OPINION IN CELL BIOLOGY

A peptidomics study reveals the impressive antimicrobial peptide arsenal of the wax moth Galleria mellonella

(2009) Susan E. Brown et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

IAPV, a bee-affecting virus associated with Colony Collapse Disorder can be silenced by dsRNA ingestion

(2009) E. Maori et al.   INSECT MOLECULAR BIOLOGY

Mechanisms of dsRNA uptake in insects and potential of RNAi for pest control: A review

(2009) Hanneke Huvenne et al.   JOURNAL OF INSECT PHYSIOLOGY

Mass spectrometric profiling of (neuro)-peptides in the worker honeybee, Apis mellifera

(2009) Bart Boerjan et al.   NEUROPHARMACOLOGY

The Gene Ontology in 2010: extensions and refinements

(2009)   NUCLEIC ACIDS RESEARCH

Quantitative peptidomics reveal brain peptide signatures of behavior

(2009) A. Brockmann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Honey bee aggression supports a link between gene regulation and behavioral evolution

(2009) C. Alaux et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

DNA methylation is widespread and associated with differential gene expression in castes of the honeybee, Apis mellifera

(2009) N. Elango et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The hemolymph proteome of the honeybee: Gel-based or gel-free?

(2009) Annelies Bogaerts et al.   PROTEOMICS

Insights into female sperm storage from the spermathecal fluid proteome of the honeybee Apis mellifera

(2009) Boris Baer et al.   GENOME BIOLOGY

Overexpression ofHmgDcauses the failure of pupariation inDrosophilaby affecting ecdysone receptor pathway

(2008) Jing Chen et al.   ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY

Prediction of neuropeptide cleavage sites in insects

(2008) Bruce R. Southey et al.   BIOINFORMATICS

Epigenetics, an emerging discipline with broad implications

(2008) Robert Feil   COMPTES RENDUS BIOLOGIES

Construction of a piggyBac-based enhancer trap system for the analysis of gene function in silkworm Bombyx mori

(2008) Keiro Uchino et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Genome-wide analysis of genes related to ovary activation in worker honey bees

(2008) G. J. Thompson et al.   INSECT MOLECULAR BIOLOGY

The Construction of a Bioactive Peptide Database in Metazoa

(2008) Feng Liu et al.   JOURNAL OF PROTEOME RESEARCH

The genome of the model beetle and pest Tribolium castaneum

(2008) Stephen Richards et al.   NATURE

Cloning and identification of an oxytocin/vasopressin-like receptor and its ligand from insects

(2008) E. Stafflinger et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A powerful method combining homologous recombination and site-specific recombination for targeted mutagenesis in Drosophila

(2008) G. Gao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases

(2008) K. J. Beumer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nutritional Control of Reproductive Status in Honeybees via DNA Methylation

(2008) R. Kucharski et al.   SCIENCE

Molecular evolution of neuropeptides in the genus Drosophila

(2008) Christian Wegener et al.   GENOME BIOLOGY

How functional genomics and genetics complements insect endocrinology

(2007) Ank Reumer et al.   GENERAL AND COMPARATIVE ENDOCRINOLOGY