Article
Infectious Diseases
Sang-Guen Kim, Sung-Bin Lee, Su-Jin Jo, Kevin Cho, Jung-Kum Park, Jun Kwon, Sib Sankar Giri, Sang-Wha Kim, Jeong-Woo Kang, Won-Joon Jung, Young-Min Lee, Eunjung Roh, Se-Chang Park
Summary: This study suggests the potential of using a combination of bacteriophages and antibiotics as an effective strategy to control the outbreak of blight disease. The combination showed improved host infectivity coverage and achieved synergistic effects in inhibiting bacterial growth. The phage cocktail demonstrated higher antibacterial activity compared to the antibiotic alone.
Article
Entomology
Wessam Hassan, Osman Ahmed, Rasha E. Hassan, Sahar A. Youssef, A. A. Shalaby
Summary: This study aimed to isolate and identify the bacteria causing fire blight disease and the corresponding bacteriophages. The results showed that bacteriophages were effective in reducing the severity of fire blight disease in pear trees, indicating their potential as a biological control method.
EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL
(2023)
Article
Agriculture, Multidisciplinary
Yeong Seok Kim, Men Thi Ngo, Bomin Kim, Jae Woo Han, Jaekyeong Song, Myung Soo Park, Gyung Ja Choi, Hun Kim
Summary: In this study, a fungal isolate with antibacterial activity against E. amylovora was identified and characterized. The active compound from the culture was found to effectively suppress the development of fire blight disease. This research provides new insights into the potential of using P. brasilianum for biocontrol of fire blight.
PLANT PATHOLOGY JOURNAL
(2022)
Article
Biology
Jaein Choe, Byeori Kim, Mi-Kyung Park, Eunjung Roh
Summary: In this study, Phi Fifi106, isolated from pear orchard soil, was purified and characterized, and its efficacy for controlling fire blight in apple plants was evaluated. The results showed that Phi Fifi106 efficiently inhibited the infection of E. amylovora and reduced the disease incidence and severity in M9 apple plants.
Article
Microbiology
Guillermo Esteban-Herrero, Belen Alvarez, Ricardo D. Santander, Elena G. Biosca
Summary: Erwinia amylovora, the bacterial species responsible for fire blight disease, causes significant economic losses in pome fruit crops worldwide. Chemical control methods are not always effective and can be harmful to the environment and human health. This study investigated the potential of using antagonistic bacteria as biocontrol agents against E. amylovora. Among the bacterial isolates tested, 12% showed antagonistic activity against the pathogen and some maintained their activity even after chloroform inactivation. Further experiments demonstrated that selected isolates were able to reduce the severity of fire blight symptoms in loquats and pears. These isolates also produced hydrolases linked to biocontrol and were able to fix molecular nitrogen. Based on additional characterization, four biocontrol strain candidates were selected and identified as Bacillus, Paenarthrobacter, and Pseudomonas genera. The results of this study provide promising prospects for improving the biological control strategies against fire blight disease.
Review
Plant Sciences
Steven Gayder, Sandrine Kammerecker, Lars Fieseler
Summary: This review summarizes the current knowledge of different phages infecting Erwinia amylovora, the causative agent of fire blight in pome fruit. Most studies have focused on in vitro efficacy, with limited data on infection assays using pear slices or detached blossoms, and few field trials reported. Further research is needed to determine if bacteriophages can effectively control fire blight in the open field.
JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Horticulture
Gulshariya Kairova, Nurzhan Daulet, Maxim Solomadin, Nurlan Sandybayev, Shynggys Orkara, Vyacheslav Beloussov, Nazym Kerimbek, Dilyara Gritsenko, Zagipa Sapakhova
Summary: This study conducted phytopathological evaluation and molecular genetic analysis of 59 apple varieties in different regions of Kazakhstan, finding that 26 varieties showed the highest resistance to fire blight. Interestingly, all 59 apple cultivars had identical genotypes for the 10 markers associated with fire blight resistance.
Article
Infectious Diseases
Annette Wimmer, Michael Glueck, Wenzi Ckurshumova, Jun Liu, Michael Fefer, Kristjan Plaetzer
Summary: This study aims to demonstrate that photodynamic inactivation can be used to treat plant diseases even if the causative bacteria are resistant to conventional treatment. Different photosensitizers in combination with different culture conditions have varying effects on bacterial inactivation, and PDI based on natural photosensitizers might represent an eco-friendly treatment strategy.
Review
Plant Sciences
Quan Zeng, Joanna Pulawska, Jeffrey Schachterle
Summary: This review examines the different infection stages of the fire blight disease caused by Erwinia amylovora, focusing on the early events during the initial infection of flowers and shoot tips. It discusses the production and transfer of inoculum, epiphytic proliferation of the pathogen, entry into the host, and initial proliferation inside the host. Additionally, the review delves into how E. amylovora modulates its virulence expression to induce disease symptoms during these early events.
JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Entomology
Matthew Boucher, Rowan Collins, Stephen Hesler, Kerik Cox, Greg Loeb
Summary: The study found that Delia platura flies prefer infected and oozing fruit over uninfected fruit, and this preference diminishes when bacterial ooze is removed. However, the flies do not show a preference between infected saplings and uninfected saplings. Additionally, the volatiles from infected fruit do not attract the flies, indicating that diseased fruit odor is not the reason for their preference.
ENVIRONMENTAL ENTOMOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Jungkum Park, Byeori Kim, Sujin Song, Yong Whan Lee, Eunjung Roh
Summary: This study isolated nine bacteriophages from orchards in Korea that were infected with fire blight. These bacteriophages showed different genetic diversity and were effective in controlling the fire blight-causing pathogen E. amylovora.
PLANT PATHOLOGY JOURNAL
(2022)
Article
Microbiology
Leandra E. Knecht, Yannick Born, Cosima Pelludat, Joel F. Pothier, Theo H. M. Smits, Martin J. Loessner, Lars Fieseler
Summary: The widespread use of antibiotics has led to an increase in antibiotic resistant bacteria. As a result, researchers are exploring effective alternatives, such as bacteriophages. However, phage resistance can still occur. This study found that different phages had varying effects on the resistance of Erwinia amylovora CFBP 1430, the bacteria responsible for fire blight. Additionally, a mutant strain, 1430(Y2R), exhibited cross-resistance to certain phages and altered LPS structures.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Duck Kyu Ryu, Mahesh Adhikari, Dong Hyuk Choi, Kyung Jin Jun, Do Hyoung Kim, Chae Ryeong Kim, Min Kyu Kang, Duck Hwan Park
Summary: Fire blight, caused by Erwinia amylovora, is a major bacterial disease in apple and pear trees, resulting in significant economic losses worldwide. This study analyzed the mechanisms and disease severity reduction efficacy of copper-based control agents (CBCAs) against E. amylovora. The results showed that copper oxychloride, copper sulfate basic, and tribasic copper sulfate at 2,000 ppm had significant reduction effects on fire blight.
PLANT PATHOLOGY JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Imen Medhioub, Manel Cheffi, Slim Tounsi, Mohamed Ali Triki
Summary: This study demonstrates the potential of Bacillus velezensis as a biological control agent against fire blight disease caused by Erwinia amylovora. B. velezensis OEE1 showed high antibacterial activity and multi-resistance to antibiotics. The active compounds extracted from B. velezensis OEE1 exhibited antibacterial activity against E. amylovora. The efficacy of B. velezensis OEE1 in reducing disease severity and plant mortality was confirmed in greenhouse and field experiments.
BIOLOGICAL CONTROL
(2022)
Article
Plant Sciences
Gaucher Matthieu, Righetti Laura, Aubourg Sebastien, Duge de Bernonville Thomas, Brisset Marie-Noelle, Chevreau Elisabeth, Vergne Emilie
Summary: The pPPO16 promoter, cloned from apple, is a useful tool for creating fire blight resistant apple genotypes without using transgenic methods. It allows fast and strong expression of transgenes in apple, making it a potential regulator.
PLANT CELL REPORTS
(2022)
Article
Engineering, Biomedical
Shalu Suri, Susan M. Lehman, Shivaram Selvam, Khalilah Reddie, Santanu Maity, Niren Murthy, Andres J. Garcia
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2015)
Letter
Cardiac & Cardiovascular Systems
Saima Aslam, Victor Pretorius, Susan M. Lehman, Sandra Morales, Robert T. Schooley
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2019)
Article
Virology
Susan M. Lehman, Gillian Mearns, Deborah Rankin, Robert A. Cole, Frenk Smrekar, Steven D. Branston, Sandra Morales
Article
Infectious Diseases
Nancy Law, Cathy Logan, Gordon Yung, Carrie-Lynn Langlais Furr, Susan M. Lehman, Sandra Morales, Francisco Rosas, Alexander Gaidamaka, Igor Bilinsky, Paul Grint, Robert T. Schooley, Saima Aslam
Article
Surgery
Saima Aslam, Andrew M. Courtwright, Christine Koval, Susan M. Lehman, Sandra Morales, Carrie-Lynn Langlais Furr, Francisco Rosas, Michael J. Brownstein, Joseph R. Fackler, Brittany M. Sisson, Biswajit Biswas, Matthew Henry, Truong Luu, Brittany N. Bivens, Theron Hamilton, Christopher Duplessis, Cathy Logan, Nancy Law, Gordon Yung, Jason Turowski, Judith Anesi, Steffanie A. Strathdee, Robert T. Schooley
AMERICAN JOURNAL OF TRANSPLANTATION
(2019)
Article
Microbiology
Razieh Kebriaei, Katherine Lev, Taylor Morrisette, Kyle C. Stamper, Jacinda C. Abdul-Matakabbir, Susan M. Lehman, Sandra Morales, Michael J. Rybak
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2020)
Article
Microbiology
Taylor Morrisette, Katherine L. Lev, Razieh Kebriaei, Jacinda C. Abdul-Mutakabbir, Kyle C. Stamper, Sandra Morales, Susan M. Lehman, Gregory S. Canfield, Breck A. Duerkop, Cesar A. Arias, Michael J. Rybak
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2020)
Article
Microbiology
Razieh Kebriaei, Katherine L. Lev, Kyle C. Stamper, Susan M. Lehman, Sandra Morales, Michael J. Rybak
Summary: This study proved the effectiveness of bacteriophage-antibiotic combinations in preventing bacteriophage resistance and enhancing bacterial eradication in simulated endocardial vegetation models, potentially combating serious MRSA infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Virology
Mart Krupovic, Dann Turner, Vera Morozova, Mike Dyall-Smith, Hanna M. Oksanen, Rob Edwards, Bas E. Dutilh, Susan M. Lehman, Alejandro Reyes, Diana P. Baquero, Matthew B. Sullivan, Jumpei Uchiyama, Jesca Nakavuma, Jakub Barylski, Mark J. Young, Shishen Du, Poliane Alfenas-Zerbini, Alla Kushkina, Andrew M. Kropinski, Ipek Kurtboke, J. Rodney Brister, Cedric Lood, B. L. Sarkar, Tong Yigang, Ying Liu, Li Huang, Johannes Wittmann, Nina Chanishvili, Leonardo J. van Zyl, Janis Rumnieks, Tomohiro Mochizuki, Matti Jalasvuori, Ramy K. Aziz, Malgorzata Lobocka, Kenneth M. Stedman, Andrey N. Shkoporov, Annika Gillis, Xu Peng, Francois Enault, Petar Knezevic, Rob Lavigne, Sung-Keun Rhee, Virginija Cvirkaite-Krupovic, Cristina Moraru, Andrea I. Moreno Switt, Minna M. Poranen, Andrew Millard, David Prangishvili, Evelien M. Adriaenssens
Summary: The Bacterial Viruses Subcommittee and the Archaeal Viruses Subcommittee of the International Committee on Taxonomy of Viruses (ICTV) summarized their activities from March 2020 to March 2021, reporting the separation of the former Subcommittee into two separate entities and the creation or redefinition of numerous new taxa, including a new realm, three orders, 15 families, 31 subfamilies, 734 genera, and 1845 species.
ARCHIVES OF VIROLOGY
(2021)
Article
Microbiology
Taylor Morrisette, Katherine L. Lev, Gregory S. Canfield, Breck A. Duerkop, Razieh Kebriaei, Kyle C. Stamper, Dana Holger, Susan M. Lehman, Smaranda Willcox, Cesar A. Arias, Michael J. Rybak
Summary: Enterococcus faecium is an important multidrug-resistant pathogen. The use of bacteriophage cocktails as a complement to antibiotic therapy has been proposed. This study selected two phages (113 and 9184) with the broadest host ranges for further experiments and found that the combination of Daptomycin and the phage cocktail showed bactericidal activity and prevented phage resistance against daptomycin-nonsusceptible E. faecium.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Dana J. Holger, Katherine L. Lev, Razieh Kebriaei, Taylor Morrisette, Rahi Shah, Jose Alexander, Susan M. Lehman, Michael J. Rybak
Summary: This study investigates the impact of phage-antibiotic combinations (PAC) on bacterial killing, resistance development, and outer membrane vesicle (OMV) production in multidrug-resistant (MDR) P. aeruginosa. The findings suggest that PAC has the potential to augment killing of MDR P. aeruginosa, prevent resistance development, and reduce OMV production.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Razieh Kebriaei, Katherine L. Lev, Rahi M. Shah, Kyle C. Stamper, Dana J. Holger, Taylor Morrisette, Ashlan J. Kunz Coyne, Susan M. Lehman, Michael J. Rybak
Summary: Bacterial biofilms are difficult to eradicate and can complicate infections. Phage+antibiotic combinations (PAC) may be more effective on biofilms than single agents. Our study demonstrated the effectiveness of phage Sb-1 on MRSA biofilms and suggested PAC regimens as adjuncts to antibiotics.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Jennifer M. Dan, Susan M. Lehman, Rita Al-kolla, Samuel Penziner, Kamyar Afshar, Gordon Yung, Eugene Golts, Nancy Law, Cathy Logan, Zsuzsanna Kovach, Gill Mearns, Robert T. Schooley, Saima Aslam, Shane Crotty
Summary: Bacteriophage therapy involves using viruses to kill antibiotic-resistant bacteria. In this case study, we observed the development of phage-specific CD4 T cells, immunoglobulin G, and neutralizing antibodies in response to bacteriophage therapy for a lung transplant recipient with multidrug-resistant Pseudomonas aeruginosa pneumonia. Despite the development of phage-specific immune responses, the treatment was considered successful. We provide a detailed profile of the immune responses in this case.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Microbiology
Amer El Ghali, Kyle Stamper, Ashlan J. Kunz Coyne, Dana Holger, Razieh Kebriaei, Jose Alexander, Susan M. Lehman, Michael J. Rybak
Summary: This study evaluated the activity of phage-ciprofloxacin combinations against drug-resistant P. aeruginosa. The results showed that CIP-LL-EC-109 combination therapy had synergistic and bactericidal activity and mitigated phage resistance in an ex vivo simulated endocardial vegetation model.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Immunology
Jennifer M. Dan, Susan M. Lehman, Rita Al-Kolla, Samuel Penziner, Kamyar Afshar, Gordon Yung, Eugene Golts, Nancy Law, Cathy Logan, Zsuzsanna Kovach, Gill Mearns, Robert T. Schooley, Saima Aslam, Shane Crotty
Summary: This study reported a case of successful treatment of multidrug-resistant Pseudomonas aeruginosa pneumonia in a lung transplant recipient using phage therapy. The development of phage-specific immune response was observed.
JOURNAL OF INFECTIOUS DISEASES
(2022)