Article
Polymer Science
Chong Teng, Zhicheng Tong, Qiulin He, Huangrong Zhu, Lu Wang, Xianzhu Zhang, Wei Wei
Summary: In this study, biocompatible hydrogel microspheres were fabricated to promote the osteogenic differentiation of stem cells and enhance bone regeneration. The results from in vivo experiments demonstrated the superiority of the BMSC/HM system in inducing bone regeneration. This study provides a simple and effective research idea for bone defect repair, and the optimization of hydrogel microspheres holds promise for future tissue engineering applications.
Article
Biochemistry & Molecular Biology
Thanuja D. K. Herath, Leonardo Saigo, Benoit Schaller, Anis Larbi, Swee Hin Teoh, Charles James Kirkpatrick, Bee Tin Goh
Summary: This study showed that autologous neutrophils may have a positive effect on promoting new bone formation, providing potential clinical benefits for bone regeneration in oral and maxillofacial surgery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Zhiwei Xu, Cunyang Wang, Guiqin Song, Yan Wang, Xiaoyun Zhang, Xiaoming Li
Summary: Covalently binding bone morphogenetic protein-2 (BMP-2)-derived peptides onto ammonized graphene (GP) can enhance the efficiency of promoting bone regeneration and repairing calvarial defect, as the positively charged ammonized GP can absorb specific proteins and interact with them effectively to induce osteogenic cell differentiation. This study demonstrates that the performance of scaffolds containing covalently bound bioactive molecules can be controlled by the covalent binding mode, and the prepared ammonized GP scaffold shows inspiring potential for bone tissue regeneration and engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Engineering, Biomedical
Meadhbha Brennan, David S. Monahan, Benedicte Brulin, Sara Gallinetti, Paul Humbert, Christina Tringides, Cristina Canal, Maria Pau Ginebra, Pierre Layrolle
Summary: In this study, the healing capacity of CDHA administering MSCs to bone defects was evaluated for the first time and compared with sintered beta-TCP constructs. Greater cell infiltration occurred in CDHA scaffolds, and after 8 weeks in vivo, MSC engraftment was higher in CDHA compared to beta-TCP, as was bone in-growth.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Patricia A. Miguez, Stephen A. Tuin, Adam G. Robinson, Joyce Belcher, Prapaporn Jongwattanapisan, Kimberly Perley, Vinicius de Paiva Goncalves, Arash Hanifi, Nancy Pleshko, Elisabeth R. Barton
Summary: Hesperidin has shown to enhance osteogenic markers and collagen matrix quality, potentially serving as an adjunct in regenerative bone therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
S. Kotagudda Ranganath, M. Schlund, Jerome Delattre, J. Ferri, F. Chai
Summary: Most existing preclinical models for evaluating the biosafety and bone-regeneration efficacy of bone substitute materials (BSMs) or tissue engineering (TE) constructs have limitations. This study developed a novel double-site critical-sized defect (CSD) model in one rabbit, which reduces the number of animals needed and avoids the influence of individual differences and evaluation bias.
MATERIALS TODAY BIO
(2022)
Article
Veterinary Sciences
Tsvetan Chaprazov, Radina Vasileva, Kiril Atliev, Elena Firkova
Summary: This study compared the effects of local and systemic application of recombinant human erythropoietin (rhEPO) on the healing of rat calvarial defects. The results showed that local application of rhEPO enhanced bone formation without systemic effects, while systemic application affected erythropoiesis but did not result in bone formation.
VETERINARY SCIENCES
(2022)
Article
Chemistry, Physical
Mindaugas Pranskunas, Egidijus Simoliunas, Milda Alksne, Victor Martin, Pedro Sousa Gomes, Algirdas Puisys, Algirdas Kaupinis, Gintaras Juodzbalys
Summary: The study evaluated the functionality of secretome isolated from periosteum-derived MSCs in healing critical size calvarial bone defects in rabbits, showing that osteogenic-induced secretome enhanced new bone formation process and had the highest bone tissue formation. The findings suggest that MSC secretome, particularly from osteogenic-induced populations, may be an effective therapeutic approach to enhance bone tissue healing and regeneration.
Article
Biochemistry & Molecular Biology
Agnieszka Kulesza, Katarzyna Zielniok, Jakub Hawryluk, Leszek Paczek, Anna Burdzinska
Summary: This study investigated the effects of nonselective COX inhibitor ibuprofen on human bone marrow MSCs (hBM-MSCs). The results showed that ibuprofen reduced PGE(2) secretion, but did not significantly affect proliferation and migration of MSCs. However, it significantly decreased the secretion of certain pro-regenerative factors.
Review
Cell Biology
Stefania Crippa, Ludovica Santi, Margherita Berti, Giada De Ponti, Maria Ester Bernardo
Summary: The human organism requires the production of approximately 1 trillion new blood cells per day through hematopoiesis in the bone marrow, regulated by the BM microenvironment and HSPCs. The BM niche, defined by interactions between HSPCs and non-hematopoietic cells, controls HSPC maintenance and orchestrates hematopoiesis according to the body's needs. Mesenchymal stromal cells (MSCs) play a key role in the BM niche by regulating HSPC homeostasis and impacting the outcome of hematopoietic stem cell transplantation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Peripheral Vascular Disease
Yunke Ren, Xinying Chu, Janaka Senarathna, Akanksha Bhargava, Warren L. Grayson, Arvind P. Pathak
Summary: This study developed a new multimodality imaging approach to characterize the remodeling vasculature and its effects on osteogenesis in a preclinical calvarial defect model. The results showed that angiogenic evolution played a crucial role in calvarial bone regeneration, preceding osteogenesis and corresponding to distinct phases of bone healing.
Article
Engineering, Biomedical
Neety Sahu, Pranay Agarwal, Fiorella Grandi, Michela Bruschi, Stuart Goodman, Derek Amanatullah, Nidhi Bhutani
Summary: The study demonstrates that co-culturing patient-derived OA cartilage explants with alginate-encapsulated MSC microbeads can lead to improved proteoglycan distribution, increased sGAG content, cell proliferation, elevated levels of cytokines such as IL-10, HGF, and sFAS, increased TIMP1 levels, and a decrease in apoptotic cells. This indicates that the paracrine factors secreted by MSC microbeads create a conducive microenvironment for anabolic, proliferative, and anti-apoptotic processes in patient-derived OA cartilage, ultimately promoting endogenous regeneration.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Multidisciplinary Sciences
Cheng Su, Xiao Zheng, Yanjin He, Li Long, Wenchuan Chen
Summary: This study identified aberrant expression of circRNAs in hUCMSCs during osteogenic differentiation, constructed competing endogenous RNA networks using bioinformatics tools, elucidated underlying mechanisms, and validated the osteogenic-promoting effect of a specific circRNA.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Elena Markina, Elena Andreeva, Ludmila Buravkova
Summary: The rodent hindlimb unloading (HU) model was used to investigate the effects of space weightlessness on bone marrow stromal precursors. Multipotent mesenchymal stromal cells (MMSCs) were isolated from rat femur and tibia and examined after periods of HU and subsequent restoration of load (HU + RL). The study found that HU led to a decrease in osteogenic activity in MMSCs, with more pronounced effects observed in the MMSCs from the tibia. These findings are important for understanding the mechanisms of skeletal disorders in astronauts during long-term space missions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Dirk Waehnert, Julian Koettnitz, Madlen Merten, Daniel Kronenberg, Richard Stange, Johannes F. W. Greiner, Christian Kaltschmidt, Thomas Vordemvenne, Barbara Kaltschmidt
Summary: Comparing different bone substitute materials in rat calvarial critical size defects, transplantation of Spongostan(TM) resulted in superior regeneration, indicating its potential as an alternative for defects in the maxillary region.
Article
Endocrinology & Metabolism
K. -H. William Lau, Charles H. Rundle, Xiao-Dong Zhou, David J. Baylink, Matilda H. -C. Sheng
Article
Biochemistry & Molecular Biology
K-H W. Lau, S-T Chen, X. Wang, S. Mohan, J. E. Wergedal, C. Kesavan, A. K. Srivastava, D. S. Gridley, S. L. Hall
Article
Endocrinology & Metabolism
Matilda H. -C. Sheng, Kin-Hing William Lau, Ram Lakhan, Abu Shufian Ishtiaq Ahmed, Charles H. Rundle, Patra Biswanath, David J. Baylink
Review
Biochemistry & Molecular Biology
Kin-Hing William Lau, Matilda H-C Sheng
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2018)
Article
Immunology
Mariam Choudry, Xiaolei Tang, Tiffany Santorian, Samiksha Wasnik, Jidong Xiao, Weirong Xing, Kin-Hing William Lau, Subburaman Mohan, David J. Baylink, Xuezhong Qin
Article
Immunology
Yi Xu, Yanmei Cheng, David J. Baylink, Samiksha Wasnik, Gati Goel, Mei Huang, Huynh Cao, Xuezhong Qin, Kin-Hing William Lau, Christian Chan, Adam Koch, Linh H. Pham, Jintao Zhang, Chih-Huang Li, Xiaohua Wang, Edmundo Carreon Berumen, James Smith, Xiaolei Tang
JOURNAL OF IMMUNOLOGY
(2019)
Article
Multidisciplinary Sciences
Samiksha Wasnik, Ram Lakhan, David J. Baylink, Charles H. Rundle, Yi Xu, Jintao Zhang, Xuezhong Qin, Kin-Hing William Lau, Edmundo E. Carreon, Xiaolei Tang
Article
Endocrinology & Metabolism
Virginia Stiffel, Charles H. Rundle, Matilda H. -C. Sheng, Subhashri Das, Kin-Hing William Lau
CALCIFIED TISSUE INTERNATIONAL
(2020)
Article
Infectious Diseases
Chih-Huang Li, Xiaolei Tang, Samiksha Wasnik, Xiaohua Wang, Jintao Zhang, Yi Xu, Kin-Hing William Lau, H. Bryant Nguyen, David J. Baylink
BMC INFECTIOUS DISEASES
(2019)
Article
Endocrinology & Metabolism
Virginia M. Stiffel, Alexander Thomas, Charles H. Rundle, Matilda H-C Sheng, Kin-Hing William Lau
CALCIFIED TISSUE INTERNATIONAL
(2020)
Article
Cell Biology
Abu Shufian Ishtiaq Ahmed, Matilda H. C. Sheng, Kin-Hing William Lau, Sean M. Wilson, Daniel Wongwarawat, Xiaolei Tang, Mahdis Ghahramanpouri, Antoine Nehme, Yi Xu, Amir Abdipour, Xiao-Bing Zhang, Samiksha Wasnik, David J. Baylink
Summary: Impaired coupled bone formation is a major cause of osteoporosis. This study suggests that the release of bone calcium during osteoclastic bone resorption is essential for coupled bone formation. Calcium signaling plays a significant role in regulating bone cell anabolic activities and is a major regulator of coupled bone formation.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Endocrinology & Metabolism
Matilda H-C Sheng, Kin-Hing William Lau, Charles H. Rundle, Anar Alsunna, Sean M. Wilson, David J. Baylink
Summary: This study reveals the mechanism of impaired osteoblast differentiation in aged bone cells, which is caused by reduced activation of SOCE. This finding is important for understanding the mechanism of fracture repair in the elderly.
JOURNAL OF BONE AND MINERAL METABOLISM
(2022)
Article
Endocrinology & Metabolism
Wanqiu Chen, Samiksha Wasnik, Yawen Fu, Leslie Aranda, Charles H. Rundle, Kin-Hing William Lau, David J. Baylink, Xiaobing Zhang
Article
Endocrinology & Metabolism
Kin-Hing William Lau, Virginia M. Stiffel, Charles H. Rundle, Mehran Amoui, Jordan Tapia, Tyler D. White, Matilda H-C Sheng
Article
Medicine, Research & Experimental
Samiksha Wasnik, Charles H. Rundle, David J. Baylink, Mohammad Safaie Yazdi, Edmundo E. Carreon, Yi Xu, Xuezhong Qin, Kin-Hing William Lau, Xiaolei Tang