Journal
BRITISH POULTRY SCIENCE
Volume 51, Issue 6, Pages 821-827Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/00071668.2010.499141
Keywords
-
Categories
Funding
- National Science Council, Republic of China [NSC92-2313-B-002-131, NSC93-2313-B-002-113, NSC95-2313-B-002-060-MY3]
Ask authors/readers for more resources
1. The purpose of this study was to investigate the effects of epidermal growth factor (EGF) on progesterone (P4) secretion from ovarian granulosa cells in Tsaiya ducks (Anas platyrhynchos var. domestica). 2. We obtained the largest (F1) follicle from Tsaiya duck, the granulosa layer was separated and the cells were isolated according to their proximity to the germinal disc. 3. The granulosa cells were cultured invitro, the culture media and the cells were used to determine P4 and steroidogenic enzyme concentrations, respectively. 4. P4 concentrations were decreased in cultured granulosa cells taken proximal to the germinal disc (GD) compared to those distal to the germinal disc (NGD). 5. EGF inhibited both basal and ovine luteinising hormone (oLH)-induced P4 concentrations. It also inhibited the P4 secretion via protein kinase A (PKA) pathway when cultured with GD and NGD granulose cells (mixed together) invitro. 6. Western blot results showed decreased concentrations of cytochrome P450 side-chain cleavage (P450scc) enzyme, 3-hydroxysteroid dehydrogenase (3-HSD) enzyme and steroid acute regulatory (StAR) protein expression, when the cells were co-treated with EGF and oLH. 7. The inhibitory effect of oLH-induced P4 production was attenuated by EGF by the addition of MAP-erk kinase (MEK) inhibitor (PD98059) suggests EGF may inhibit P4 production by affecting via the mitogen-activated protein kinase (MAPK) pathway.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available