Article
Parasitology
Elise Courtot, Morgane Miclon, Barbara Reaves, Adrian J. Wolstenholme, Cedric Neveu
Summary: Levamisole is a broad-spectrum anthelmintic used to control parasitic nematodes impacting livestock, but its efficacy is compromised by drug-resistant parasites. This study investigates truncated cholinergic receptor sub-units as potential levamisole resistance markers in nematodes. The researchers used Xenopus oocyte and Caenorhabditis elegans as expression systems to functionally investigate these markers. They found that truncated UNC-63 induces a reduction in levamisole susceptibility, providing the first functional validation for this molecular marker.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2023)
Article
Multidisciplinary Sciences
Jeba R. J. Jesudoss Chelladurai, Katy A. Martin, Pam Vardaxis, Craig Reinemeyer, Paramasivan Vijayapalani, Alan P. Robertson, Matthew T. Brewer
Summary: In this study, the researchers investigated the role of permeability glycoproteins (P-gps) in Toxocara canis drug tolerance. They found that T. canis has 13 annotated P-gp genes, with at least 10 expressed in adults and hatched larvae, and at least 8 expressed in somatic larvae. However, treatment with macrocyclic lactones did not significantly increase P-gp expression. Further studies are needed to understand the contribution of P-gps to macrocyclic lactone tolerance in T. canis.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Amanda C. Shaver, Janneke Wit, Clayton Dilks, Timothy Crombie, Hanchen Li, Raffi Aroian, Erik Andersen
Summary: Treatment of parasitic nematode infections in humans and livestock is becoming a challenge due to the increasing anthelmintic resistance. However, the use of Caenorhabditis elegans as a model organism has provided insights into the molecular targets of anthelmintic drugs. By studying genetically diverse strains of C. elegans, researchers were able to identify variations in anthelmintic responses and quantify the contribution of genetics to resistance. This study highlights the importance of using genetically diverse models to understand anthelmintic resistance.
Article
Multidisciplinary Sciences
Daniela Bedenice, Jessica Resnick-Sousa, Lauren Bookbinder, Victoria Trautwein, Hannah N. Creasey, Giovanni Widmer
Summary: This study examined the correlation between nematode worm burden and intestinal microbiota composition in adult alpacas. Fecal samples were collected from 102 healthy alpacas at three different time points. The results showed high temporal stability in the fecal microbiota of healthy alpacas. The intensity of nematode infection was only significantly correlated with microbiota composition 14 days after treatment. Alpaca age was found to be the only consistent predictor of microbiota taxonomic composition.
Article
Veterinary Sciences
Michal Babjak, Alzbeta Konigova, Michaela Komaromyova, Tetiana Kuzmina, Pawel Nosal, Marian Varady
Summary: This study was conducted to investigate the resistance of Haemonchus contortus to albendazole and ivermectin. The results showed reduced efficacy of albendazole and ivermectin, while levamisole was found to be an efficient anthelmintic treatment.
JOURNAL OF VETERINARY RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Z. J. Quintero-Elena, L. Aguilar-Marcelino, G. S. Castaneda-Ramirez, O. Gomez-Rodriguez, E. Villar-Luna, G. Lopez-Guillen, S. G. Ramirez-Rojas
Summary: The study evaluated the in vitro and micro-plot predatory activity of the mite Caloglyphus mycophagus against Haemonchus contortus (L3) and Nacobbus aberrans (J2). Results showed that the mite had in vitro predation activity against both nematodes, with significant reduction percentages. Additionally, mortality of mites in micro-plots containing ivermectin was 100%.
BIOLOGICAL CONTROL
(2022)
Article
Microbiology
Kathryn S. Evans, Janneke Wit, Lewis Stevens, Steffen R. Hahnel, Briana Rodriguez, Grace Park, Mostafa Zamanian, Shannon C. Brady, Ellen Chao, Katherine Introcaso, Robyn E. Tanny, Erik C. Andersen
Summary: Researchers used Caenorhabditis elegans as a model organism to study macrocyclic lactone (ML) resistance, identifying three genomic regions on chromosome V that contribute to natural differences in response to abamectin. Two novel loci were discovered, likely representing new resistance mechanisms. By comparing genes in these new loci to those in the parasitic nematode Haemonchus contortus, potential shared resistance genes between nematode species were identified. Overall, this study highlights the advantages of using C. elegans to understand anthelmintic resistance in parasitic nematodes.
Article
Biotechnology & Applied Microbiology
Ricardo Jose Comans-Perez, Jose E. Sanchez, Laith Khalil Tawfeeq Al-Ani, Manases Gonzalez-Cortazar, Gloria Sarahi Castaneda-Ramirez, Pedro Mendoza-de Gives, Alma Delia Sanchez-Garcia, Jair Millan-Orozco, Liliana Aguilar-Marcelino
Summary: This study evaluated the morphology of ten strains of edible mushrooms and their anthelmintic activity against Haemonchus contortus larvae in vitro. The results showed that some fungi exhibited high toxicity against nematodes, making them potential candidates for future in vivo tests.
BIOLOGICAL CONTROL
(2021)
Article
Agriculture, Dairy & Animal Science
Waresi Tuersong, Xin Liu, Yifan Wang, Simin Wu, Peixi Qin, Shengnang Zhu, Feng Liu, Chunqun Wang, Min Hu
Summary: This study explored the metabolome of an IVM-resistant H. contortus strain and analyzed the role of metabolism in IVM resistance. The results showed that amino acid metabolism may be mediated by the uptake of IVM and related to IVM resistance in H. contortus. This study contributes to our understanding of the mechanisms of IVM resistance and may provide effective approaches to manage infection by resistant strains of H. contortus.
Article
Biochemistry & Molecular Biology
Chunqun Wang, Wenjie Gao, Shi Yan, Xing-Quan Zhu, Xun Suo, Xin Liu, Nishith Gupta, Min Hu
Summary: N-glycosylation is a physiologically vital post-translational modification in eukaryotic organisms, and Haemonchus contortus has exclusive chitobiose modifications in its N-glycans. Immunogenic proteins like peptidases are N-glycosylated in the parasite, with glycan-rich conjugates primarily located in the intestine and gonads of adult worms, evading host immune responses. This comprehensive atlas of N-glycosylation in the prevalent parasitic nematode highlights its importance for infection, immunity, and prevention.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Parasitology
Paulius Baltrusis, Claude L. Charvet, Peter Halvarsson, Sofia Mikko, Johan Hoglund
Summary: The nematode Haemonchus contortus is a common and pathogenic parasite in small ruminants, and detecting resistance to levamisole in this parasite is challenging. A new droplet digital PCR assay was developed to detect a specific gene deletion associated with levamisole resistance in H. contortus.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
(2021)
Article
Plant Sciences
Sasha Hayes, Aya C. Taki, Kah Yean Lum, Joseph J. Byrne, Jonathan M. White, Merrick G. Ekins, Robin B. Gasser, Rohan A. Davis
Summary: This study identified a marine extract derived from an Australian sponge with activity against an economically important parasitic nematode. Further analysis led to the purification of four known sesterterpenes and the revision of their chemical structures. The findings suggest that these sesterterpenes deserve further investigation for their activity against nematodes.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Parasitology
Laura Mate, Mariana Ballent, Candela Canton, Carlos Lanusse, Laura Ceballos, Luis L. Ignacio Alvarez, Juan Pedro Liron
Summary: This study compared the gene expression levels of ABC transporters in sheep nematode Haemonchus contortus isolates with different susceptibility to ivermectin and found that some ABC transporter genes showed minor differences in transcription levels, but could not fully explain ivermectin resistance. Pgp-9 may be one of the most relevant candidates contributing to the multi-genic nature of ivermectin resistance.
VETERINARY PARASITOLOGY
(2022)
Article
Cell Biology
Stephen R. Doyle, Roz Laing, David Bartley, Alison Morrison, Nancy Holroyd, Kirsty Maitland, Alistair Antonopoulos, Umer Chaudhry, Ilona Flis, Sue Howell, Jennifer McIntyre, John S. Gilleard, Andy Tait, Barbara Mable, Ray Kaplan, Neil Sargison, Collette Britton, Matthew Berriman, Eileen Devaney, James A. Cotton
Summary: Understanding the genetic basis of anthelmintic drug resistance in parasitic nematodes is crucial for improving parasite control. By using an in vivo genetic cross, this study successfully mapped resistance loci for three major classes of anthelmintics and identified new alleles for resistance. The study also implicated a transcription factor in ivermectin resistance and demonstrated the feasibility of high-resolution forward genetics in parasitic nematodes.
Article
Parasitology
Waresi Tuersong, Caixian Zhou, Simin Wu, Peixi Qin, Chunqun Wang, Wenda Di, Lu Liu, Hui Liu, Min Hu
Summary: This study identified differentially expressed genes (DEGs) in Ivermectin (IVM)-resistant Haemonchus contortus compared to susceptible strains, providing insights into the molecular mechanisms of IVM resistance. The study revealed potential new target genes related to lipid metabolism, cuticle structure, and important pathways such as antigen processing, lysosome, autophagy, apoptosis, and NOD1-like receptor signaling pathways. These findings contribute to a better understanding of IVM resistance in H. contortus.
PARASITES & VECTORS
(2022)
Article
Biology
Ines Gonzalez-Calvo, Keerthana Iyer, Melanie Carquin, Anouar Khayachi, Fernando A. Giuliani, Severine M. Sigoillot, Jean Vincent, Martial Seveno, Maxime Veleanu, Sylvana Tahraoui, Melanie Albert, Oana Vigy, Celia Bosso-Lefevre, Yann Nadjar, Andrea Dumoulin, Antoine Triller, Jean-Louis Bessereau, Laure Rondi-Reig, Philippe Isope, Fekrije Selimi
Summary: The study revealed the importance of Susd4 gene in synaptic function and plasticity, with its constitutive loss affecting motor coordination adaptation and learning in mice. Additionally, SUSD4 was found to interact with proteins regulating AMPA receptor turnover, potentially playing a role in neurodevelopmental diseases.
Article
Multidisciplinary Sciences
Paul Vigne, Clotilde Gimond, Celine Ferraris, Anne Vielle, Johan Hallin, Ania Pino-Querido, Sonia El Mouridi, Laure Mignerot, Christian Frokjaer-Jensens, Thomas Boulin, Henrique Teotonio, Christian Braendle
Summary: The study reveals that a single amino acid change in a potassium channel subunit can lead to matricidal hatching in nematodes by reducing the sensitivity to environmental stimuli triggering egg-laying, and this trait can be maintained under fluctuating resource availability.
Article
Genetics & Heredity
Annie Laquerriere, Dana Jaber, Emanuela Abiusi, Jerome Maluenda, Dan Mejlachowicz, Alexandre Vivanti, Klaus Dieterich, Radka Stoeva, Loic Quevarec, Flora Nolent, Valerie Biancalana, Philippe Latour, Damien Sternberg, Yline Capri, Alain Verloes, Bettina Bessieres, Laurence Loeuillet, Tania Attie-Bitach, Jelena Martinovic, Sophie Blesson, Florence Petit, Claire Beneteau, Sandra Whalen, Florent Marguet, Jerome Bouligand, Delphine Heron, Geraldine Viot, Jeanne Amiel, Daniel Amram, Celine Bellesme, Martine Bucourt, Laurence Faivre, Pierre-Simon Jouk, Suonavy Khung, Sabine Sigaudy, Anne-Lise Delezoide, Alice Goldenberg, Marie-Line Jacquemont, Laetitia Lambert, Valerie Layet, Stanislas Lyonnet, Arnold Munnich, Lionel Van Maldergem, Juliette Piard, Fabien Guimiot, Pierre Landrieu, Pascaline Letard, Fanny Pelluard, Laurence Perrin, Marie-Helene Saint-Frison, Haluk Topaloglu, Laetitia Trestard, Catherine Vincent-Delorme, Helge Amthor, Christine Barnerias, Alexandra Benachi, Eric Bieth, Elise Boucher, Valerie Cormier-Daire, Andree Delahaye-Duriez, Isabelle Desguerre, Bruno Eymard, Christine Francannet, Sarah Grotto, Didier Lacombe, Fanny Laffargue, Marine Legendre, Dominique Martin-Coignard, Andre Megarbane, Sandra Mercier, Mathilde Nizon, Luc Rigonnot, Fabienne Prieur, Chloe Quelin, Hanitra Ranjatoelina-Randrianaivo, Nicoletta Resta, Annick Toutain, Helene Verhelst, Marie Vincent, Estelle Colin, Catherine Fallet-Bianco, Michele Granier, Romulus Grigorescu, Julien Saada, Marie Gonzales, Anne Guiochon-Mantel, Jean-Louis Bessereau, Marcel Tawk, Ivo Gut, Cyril Gitiaux, Judith Melki
Summary: In this study, genetic diagnosis was achieved in 52.7% of AMC index patients, with the identification of nine newly discovered genes. The primary cause of AMC was determined to be skeletal muscle involvement (40%), with autosomal recessive inheritance being the most frequent mode (66.3%). In sporadic cases born to non-consanguineous parents, de novo dominant autosomal or X linked variants were observed in 50% of cases.
JOURNAL OF MEDICAL GENETICS
(2022)
Article
Pharmacology & Pharmacy
Nicolas Lamassiaude, Elise Courtot, Angelique Corset, Claude L. Charvet, Cedric Neveu
Summary: This study identified and characterized highly conserved GluCl subunits from different nematode species, revealing the pivotal role of GLC-2 subunit in the composition of heteromeric GluCls and discovering a novel GluCl subtype combining GLC-2/GLC-3 subunits. The findings provide new insights into GluCl diversity and potential drug targets for the development of next generation anthelmintic compounds.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tina V. A. Hansen, Heinz Sager, Celine E. Toutain, Elise Courtot, Cedric Neveu, Claude L. Charvet
Summary: This study investigates the pharmacological characteristics of the DEG-3/DES-2 channel in C. elegans. The results show that the channel is more sensitive to betaine than ACh and choline, but less sensitive to monepantel and monepantel sulfone. These findings provide important insights into the function of the DEG-3/DES-2 channel in C. elegans.
Article
Pharmacology & Pharmacy
Susanna Pucci, Cristiano Bolchi, Francesco Bavo, Marco Pallavicini, Massimiliano Renzi, Sergio Fucile, Roberta Benfante, Donatella Lattuada, Jean-Louis Bessereau, Manuela D'Alessandro, Valerie Risson, Michele Zoli, Francesco Clementi, Cecilia Gotti, Clara De Palma, Simona Di Lascio
Summary: Glioblastomas, the most common brain tumors, have a poor prognosis. Combination treatment with a compound called MG624, along with a pro-oxidant mitocan, has led to the development of two new compounds (StN-4 and StN-8) that are more effective in reducing the viability of glioblastoma cells. The anti-tumoral effects of these compounds are not solely due to their action on nicotine receptors, but also involve other mechanisms.
PHARMACOLOGICAL RESEARCH
(2022)
Review
Neurosciences
Ines Gonzalez-Calvo, Melissa Cizeron, Jean-Louis Bessereau, Fekrije Selimi
Summary: The domains CUB, CCP, and TSP-1 are fundamental building blocks of synaptic architecture, playing crucial roles in various species. Their function is partially conserved in more complex nervous systems.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Cell Biology
Charline Roy, Laurent Molin, Allan Alcolei, Mathilde Solyga, Benjamin Bonneau, Camille Vachon, Jean-Louis Bessereau, Florence Solari
Summary: DAF-2/IIRc plays a role in the relationship between longevity and motility; inactivation of DAF-2/IIRc in neurons or intestine extends the lifespan of C. elegans; the motility pattern of daf-2 mutants is determined by the sequential and opposing impact of neurons and muscle tissues.
Article
Multidisciplinary Sciences
Maelle Jospin, Benjamin Bonneau, Viviane Laine, Jean-Louis Bessereau
Summary: The properties of Caenorhabditis elegans levamisole-sensitive acetylcholine receptors (L-AChRs) can be modified by their clustering machinery, which is important for the receptors' functionality in their native environment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Genetics & Heredity
Kota Mizumoto, Yishi Jin, Jean-Louis Bessereau
Summary: Using the nematode Caenorhabditis elegans as a model organism, research has uncovered key regulators in the formation of functional synapses, highlighting the conservation of signaling networks across animals. This research contributes to advancing our understanding of the brain.
Article
Agriculture, Dairy & Animal Science
Jacques Cabaret, Melodie Mercier, Maurice Mahieu, Gisele Alexandre
Summary: Parasites, particularly gastrointestinal nematodes (GINs), pose a significant health threat to tropical goat husbandry. However, the use of synthetic anthelmintics has led to the development of resistance, necessitating the need for targeted treatments. Simple indicators such as anemia, weight loss, and hair roughness can be used to identify animals in need of treatment. The promotion of these indicators and the support of agricultural advisory services are crucial for their successful implementation.
Article
Multidisciplinary Sciences
Patricia Izquierdo, Claude Charvet, Cedric Neveu, A. Christopher Green, John E. H. Tattersall, Lindy Holden-Dye, Vincent O'Connor
Summary: In this study, the nematode C. elegans was used as a model organism to investigate the neurotoxic effects of organophosphates, particularly paraoxon-ethyl. The authors found that modulation of acetylcholine receptor function can mitigate the toxic effects of organophosphate poisoning. Mutants with reduced acetylcholine receptor activity were identified as being more resistant to the toxic consequences of organophosphate intoxication.
Article
Parasitology
Paulius Baltrusis, Peter Halvarsson, Claude L. Charvet, Johan Hoglund
Summary: Parasitic sheep nematodes, especially Haemonchus contortus, cause significant damage to animals due to their drug resistance capability. However, the mechanism of resistance to compounds like levamisole and efficient resistance detection tools are still not well understood. In this study, the presence and frequency of a newly reported mutation associated with levamisole resistance were investigated in different isolates of H. contortus. The results suggest that the mutation causing the S168T substitution in hco-acr-8 may be a plausible variant associated with levamisole resistance in some isolates.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
(2023)
Article
Agriculture, Dairy & Animal Science
Eric R. Morgan, Anne Segonds-Pichon, Hubert Ferte, Patrick Duncan, Jacques Cabaret
Summary: Parasite overdispersion among hosts has important implications for their population dynamics and control. The factors determining parasite overdispersion, such as parasite acquisition and acquired host immunity, have not been thoroughly studied in parasite populations.
Article
Agriculture, Dairy & Animal Science
Caroline Chylinski, Jacques Cortet, Jacques Cabaret, Alexandra Blanchard
Summary: Gastrointestinal nematodes (GIN) utilize flexible life history strategies to adapt to environmental challenges, and their fitness can be maintained by adjusting subsequent life traits. Understanding how distinct populations of GIN species respond to environmental challenges is important for parasite control interventions. This study compared the life history traits and fitness outcomes of three Haemonchus contortus isolates exposed to challenges during different life stages. The findings demonstrate isolate-specific alterations to life history strategies and provide insights into non-genetic adaptive processes that may affect parasite control strategies.