Article
Chemistry, Analytical
Li Li, Zheng Zhou, Xinmin Li, Dandan Li, Min Zhao, Changjin Liu, Haiping Wu, Wei Yang, Shijia Ding, Bo Shen
Summary: A novel 3D walking nanomachine-based biosensor utilizing strand displacement for ultrasensitive detection of ATP was successfully developed, demonstrating a low limit of detection and practical application in human serum samples. This biosensing strategy holds great potential for biochemical studies and clinical diagnosis.
Article
Chemistry, Analytical
Ni Liao, Mei-Chen Pan, Li Wang, Fan Yang, Ruo Yuan, Ying Zhuo
Summary: A novel swing arm location-controllable DNA walker based on DNA tetrahedral nanostructures was developed for nucleic acid detection, demonstrating enhanced efficiency and potential in diagnostic analysis.
ANALYTICAL CHEMISTRY
(2021)
Review
Engineering, Environmental
Dong Han, Kui Yang, Shiguo Sun, Jia Wen
Summary: This review provides a comprehensive summary of signal amplification strategies in ECL biosensors, including electron transfer and energy transfer-based amplification, DNA technology-based amplification, nanomaterials-based amplification, and other amplification strategies. Future perspectives and current challenges in signal amplification of ECL biosensors are also outlined.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Qirong Chen, Huahui Gao, Jianglong Yao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a new target/aptamer binding-induced inhibition of enzyme activity strategy was used to construct an amplified and sensitive electrochemical biosensor for detecting Sonic Hedgehog (SHh). The sensor exhibited high selectivity and could detect SHh down to 3.34 pM. This strategy has the potential to be a convenient approach for detecting different proteins in biological studies and disease diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Ruo Yuan, Ya-Qin Chai, Jia-Hang Li, Jia-Li Liu, Xiao-Long Zhang, Xiao-Chun Zhu
Summary: A novel ultrasensitive electrochemiluminescence (ECL) biosensor was developed using 2D Co3O4 nanosheets as a coreaction accelerator for the detection of miRNA-21. The Co3O4 nanosheets promoted the decomposition of H2O2 to generate more O2．-, which enhanced the ECL signals by reacting with luminol. Additionally, the biosensor achieved ultrasensitive detection of miRNA-21 through the self-assembly of highly ordered rolling DNA nanomachines.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Ruiying Yang, Guihua Jiang, Huimin Liu, Leiliang He, Fei Yu, Li'e Liu, Lingbo Qu, Yongjun Wu
Summary: The dual-model biosensor utilizing a 3D DNA walker strategy achieved highly sensitive detection of miRNA-224, avoiding interference and significantly increasing sensitivity through distance-controllable signal enhancing and quenching strategies.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Yuanyuan Yue, Songtao Wang, Qiang Jin, Nan An, Lina Wu, He Huang
Summary: This article presents a CRISPR/Cas12a-based electrochemical biosensor (E-CRISPR) for detecting lead ions (Pb2+), which utilizes GR-5 DNAzyme as a signal medium to convert Pb2+ into nucleic acid signals and achieves cooperative signal amplification by activating CRISPR/Cas12a cleavage of the electrochemical signal probe, enabling ultrasensitive Pb2+ detection.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Guoqiao Huang, Jing Zhang, Yinghao Cheng, Chan Li, Qiufeng Song, Chang Xue, Zai-sheng Wu, Zhifa Shen
Summary: An intelligent DNA machine called S-nanoprobe was designed for the detection of miRNA-21. The S-nanoprobe demonstrated high efficiency in detecting target miRNA and exhibited a high specificity. It could be used to detect down to 1 fM of target and could discriminate target miRNA from homologous miRNAs. The S-nanoprobe also showed promising results in detecting tumor-associated biomarkers in early diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Huifeng Xu, Shiqi Zhang, Ting Zhang, Weihua Huang, Yuting Dai, Ruoxi Zheng, Guangwen Wu
Summary: An immobilization-free ECL biosensor was constructed for ultra-trace detection of Cd2+ by using exquisite hairpin DNA and magnetic Fe3O4-graphene oxide nanosheets as the carrier. The concentration of Cd2+ was monitored based on the attenuation of ECL signal, showing high sensitivity with a detection limit of 1.1 x 10(-4) ppb.
Article
Biophysics
Zhen Zhao, Zhiqiang Chen, Dengren Liu, Li Wang, Shufeng Liu
Summary: A self-propelled DNA machine for enzyme-free and ultrasensitive electrochemical detection of nucleic acid was developed using a new strategy of fuel strand-powered target recycling and successive proximity-based inter-strand displacement. The system showed tunable detection performance and achieved a low detection limit of 0.1 fM, making it a powerful tool for amplified detection of nucleic acid with potential applications in bioanalysis, disease diagnosis, and biomedicine.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Yuan Zhang, ShuTing Li, JingJing Tian, Kai Li, Zaihui Du, WenTao Xu
Summary: A universal, highly sensitive and visual Salmonella detection method was proposed in this research, using UL-PCR-triggered SDA. This method could detect Salmonella genome at levels as low as 22 copies mL(-1), with an excellent linear range between 10(2) copies mL(-1) and 10(7) copies mL(-1). The strategy shows promise for broad applications.
Article
Biophysics
Ke Yang, Jining Li, Marc Lamy de la Chapelle, Guorong Huang, Yunxia Wang, Jinbao Zhang, Degang Xu, Jianquan Yao, Xiang Yang, Weiling Fu
Summary: A novel THz biosensor based on metamaterials coupled with nanoparticles and strand displacement amplification (SDA) was developed for sensitive detection of microRNA (miRNA) samples. The biosensor showed good detection sensitivity with a limit of detection of 14.54 aM and exhibited a linear response for miRNA-21, offering potential applications in nucleic acid analysis and cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Xianghui Li, Yilei Lu, Yiming Chen, Zhipeng Pan, Ni Lin, Zhenyu Lin, Huixing Chen
Summary: A signal-on low background electrochemiluminescence (ECL) biosensor for microRNA-141 (miRNA-141) detection was developed based on the electrostatic attraction difference between negatively charged report probes and positively charged electrode. The biosensor utilized a modified indium tin oxide (ITO) electrode with a positively charged surface to achieve low background signal by electrostatic repulsion with dichlorotris (1,10-phenanthroline) ruthenium (II) hydrate (Ru(phen)(3)(2+)). The target triggered a cascade reaction to generate negatively charged long dsDNAs, which efficiently loaded Ru(phen)(3)(2+) (Ru-dsDNA) and resulted in a strong ECL signal.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Mengnan Fan, Jianru Yang, Xiaosu Wang, Yongjie Xu, Bing Li, Hui Yang, Qin Lu, Xun Min, Meirong Huang, Jian Huang
Summary: Neisseria gonorrhoeae is the only pathogen that causes gonorrhea. A developed detection method based on recombinase polymerase amplification (RPA) can eliminate false-positive signals and has the advantages of rapid performance, high sensitivity, and minimal dependency on equipment.
ANALYTICA CHIMICA ACTA
(2023)
Article
Nanoscience & Nanotechnology
Yaqi Liu, Fengyi Wang, Shuo Ge, Lu Zhang, Zuhao Zhang, Yunqing Liu, Yan Zhang, Shenguang Ge, Jinghua Yu
Summary: A strand displacement amplification (SDA)-assisted CRISPR/Cas12a electrochemiluminescence (ECL) biosensor was developed for ultrasensitive detection of Staphylococcus aureus (Sa)-16S rDNA. The biosensor utilized a porphyrinic Zr metal-organic framework (MOF) as a coreactant accelerator to enhance the ECL emission signal. The presence of Sa-16S rDNA triggered the SDA reaction, generating single-stranded DNA products (SP) that activated the CRISPR/Cas12a trans-cleavage and effectively extinguished the ECL signals. The ECL platform exhibited high sensitivity and specificity, making it suitable for clinical diagnosis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
