Article
Biochemical Research Methods
Olivia Gordon, Joshua Gibbons, Jared Lamp, Andrew W. Lantz
Summary: There is a great need for fast, affordable, and accurate methods for microbial analysis in various fields. This study presents a novel capillary isotachophoresis (CITP) method for quantifying intact cells in mixed samples based on their electrophoretic mobility ranges. The method uses ion spacers to create efficient cell peaks in the electropherogram, which can be used to determine the concentrations of different cell species. The results demonstrate the potential application of CITP in cell growth studies, microbial contamination testing, and sterility testing.
Article
Biophysics
Hui Wang, Cuifang Liu, Xiaoting Zhang, Tiancong Xiu, Ping Li, Wei Zhang, Wen Zhang, Xin Wang, Zhenzhen Liu, Bo Tang
Summary: Understanding the pathogenesis of hypertension and exploring new therapeutic targets are crucial for developing more targeted treatments. This study developed a fluorescence probe to detect Golgi ROS levels in hypertensive mice and evaluated the effects of antihypertensive drugs. The study also identified ROS post-transcriptional modification sites on DHFR, providing further insights into the mechanisms of hypertension.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biophysics
Hui Wang, Cuifang Liu, Xiaoting Zhang, Tiancong Xiu, Ping Li, Wei Zhang, Wen Zhang, Xin Wang, Zhenzhen Liu, Bo Tang
Summary: Investigating the use of a fluorescence probe to detect increased Golgi ROS levels in hypertensive mice, evaluating the efficacy of antihypertensive drugs, and identifying ROS post-transcriptional modification sites on dihydrofolate reductase. This suggests a new therapeutic target for hypertension and the development of new antihypertensive drugs.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemical Research Methods
Kazuki Hata, Noriko Nonaka, Nobuyuki Sato, Takashi Kaneta
Summary: By adding organic solvent and adjusting the apparent pH, 17 inorganic and organic anions were successfully separated. Methanol was chosen as the modifier that provided the largest separation window to achieve complete separation of the target analytes.
Article
Chemistry, Analytical
Si-Yuan Wang, Jun-Ru Liu, Zheng-Hua Ju, Di-Hua Tian, Zuo-Hu Chai, Yu Zhang, Fang Dai, Shengxiang Zhang, Bo Zhou
Summary: A fluorescent probe that can simultaneously sense cellular microenvironment parameters and redox signaling molecules has a wide range of applications. A two-photon probe V-OS was constructed to enable simultaneous intravital imaging of mitochondrial viscosity and superoxide radical anion (O2•-). By triggering different responses at different wavelengths, V-OS can detect changes in viscosity and O2•- levels. Using this probe, the cross-talk between viscosity and O2•- was identified, and the burst of O2•- and increased viscosity during cellular ferroptosis and cerebral ischemia reperfusion injury in living mice were successfully mapped.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Agricultural Engineering
Sara El Kharraf, Soukaina El-Guendouz, Abdellah Farah, Bahia Bennani, Maria C. Mateus, El Mestafa El Hadrami, M. Graca Miguel
Summary: This study aimed to investigate the antibacterial activities of two essential oils against multiple bacteria, and the results showed that essential oils obtained by simultaneous extraction had better antimicrobial and antioxidant activities.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Magdalena Kwolek-Mirek, Aleksandra Dubicka-Lisowska, Sabina Bednarska, Renata Zadrag-Tecza, Pawel Kaszycki
Summary: Copper-zinc superoxide dismutase (SOD1) is an antioxidant enzyme that regulates oxidative stress-related alterations in the phenotype of the yeast Saccharomyces cerevisiae. Comparative proteomic analysis of the wild-type strain and Delta sod1 mutant revealed differential expression of proteins related to carbon metabolism, amino acid and protein biosynthesis, nucleotide biosynthesis, and antioxidant processes. The study suggests that protein comparative profiling of the Delta sod1 yeast can provide valuable insights into the systemic alterations in the S. cerevisiae phenotype caused by the SOD1 mutation.
Article
Pharmacology & Pharmacy
Abdollah Abdollahi-Aghdam, Mir Reza Majidi, Hadi Veladi, Yadollah Omidi
Summary: A new microfluidic-based method with electrochemical detection was developed for the simultaneous quantification of AP and PHE in human blood and pharmaceuticals. The method offers advantages of rapid analysis, simplicity, and reliable results.
Article
Chemistry, Analytical
Wen Zhang, Chunmiao Zhou, Ping Li, Jihong Liu, Xin Wang, Wei Zhang, Hui Wang, Bo Tang
Summary: Dysregulated redox homeostasis under pathological conditions can lead to oxidative stress and associated disease damage. However, the lack of real-time methods for analyzing redox states is a barrier to understanding pathogenic mechanisms. We developed a novel imaging strategy that allows real-time imaging of dual molecular events during oxidative stress, providing insights into dynamic intracellular changes of the redox state and elucidating the molecular mechanisms of oxidative stress-related diseases.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Jianjing Zhou, Zhongqi Xu
Summary: A novel capillary electrophoresis-Capacitively coupled contactless conductivity detection (CE-(CD)-D-4) method for the separation of 12 antibiotics has been developed, with formic acid used as the background electrolyte for improved cost-effectiveness and shorter analysis time. The method shows good sensitivity for both qualitative analysis and detection, allowing for the separation and detection of pharmaceutical formulations with different UV absorbing properties in a single run.
ANALYTICAL METHODS
(2022)
Article
Biology
E. A. Machigov, E. V. Igonina, D. A. Sviridova, A. V. Rubanovich, S. K. Abilev
Summary: The study investigates the effect of Paraquat on E. coli using biosensors. It is found that Paraquat generates superoxide radical anion and hydrogen peroxide, leading to decreased bacterial viability and luminescence intensity. Antioxidants are able to reduce the concentration of free radicals generated by Paraquat and increase bacterial viability.