Review
Plant Sciences
Elizabeth Ihien Katche, Annaliese S. Mason
Summary: Resynthesized rapeseed lines have recreated the historical hybridization between Brassica rapa and B. oleracea, providing valuable genetic resources for rapeseed breeding and insights into polyploid plant meiosis evolution. This review discusses the production of these lines through interspecific crosses, their resulting fertility and agronomically important traits, meiotic instability issues, and the potential role of meiosis genes and gene regulatory networks in stabilizing meiosis. The direct use of resynthesized and semi-resynthesized Brassica napus for hybrid breeding and the study of other agronomic traits is also explored.
CRITICAL REVIEWS IN PLANT SCIENCES
(2023)
Review
Genetics & Heredity
Soo-In Sohn, Senthil Kumar Thamilarasan, Subramani Pandian, Young-Ju Oh, Tae-Hun Ryu, Gang-Seob Lee, Eun-Kyoung Shin
Summary: Interspecific hybridization is common in nature and has great potential in agricultural systems. However, it can also lead to the escape of transgenes in genetically modified crops, posing risks to unintended plants. This review focuses on the interspecific hybridization and transgene escape of Brassica napus and B. rapa, aiming to better understand the environmental impact of interspecific hybrids and effectively manage transgene escape.
Article
Plant Sciences
Ying Zhou, Meicui Yang, Shihui Zhao, Haoran Shi, Yun Li, Wanzhuo Gong, Jin Yang, Jisheng Wang, Qiong Zou, Lanrong Tao, Zeming Kang, Rong Tang, Shixing Guo, Shaohong Fu
Summary: Interspecific hybridization of rapeseed is an important way to innovate breeding resources. This research successfully obtained interspecific maternal inbred offspring (induced F-1) by artificially synthesizing F-1 through artificial pollination between Brassica napus and Brassica raga. The study found that the genetic stability of egg cells was greater than that of sperm cells and the induced F-1 had normal fertility and meiosis. Genetic variation between interspecies could be preserved or broadened in the induced F-1.
Article
Biochemistry & Molecular Biology
Soo-In Sohn, Senthil Kumar Thamilarasan, Subramani Pandian, Young-Ju Oh, Hyeon-Jung Kang, Eun-Kyoung Shin
Summary: Interspecific hybridization between GM Brassica napus and Brassica rapa can result in viable hybrids that can actively hybridize up to F-3 generations or more. This suggests that transgene flow from GM B. napus to B. rapa is highly possible, and can occur through hand pollination in a greenhouse environment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Zongxiang Zhan, Nadil Shah, Ru Jia, Xiaonan Li, Chunyu Zhang, Zhongyun Piao
Summary: In this study, the clubroot resistance gene CRd was introduced from Chinese cabbage to Brassica napus using interspecific hybridization and backcrossing with molecular marker-assisted selection. The resulting clubroot-resistant variety, Zhongshuang 11R, showed resistance to P. brassicae pathotypes and had similar agronomic traits to its parental line, while meeting the quality requirements for commercial rapeseed oil.
Article
Plant Sciences
Li Zhang, Xiangsheng Li, Lichun Chang, Tianpeng Wang, Jianli Liang, Runmao Lin, Jian Wu, Xiaowu Wang
Summary: This study successfully increased the genetic diversity of Brassica juncea by introgressing the A genome of Brassica rapa. New B. juncea germplasm with expanded phenotypic variations was developed, and the introgression of B. rapa segments was tracked using SNP markers. The results showed that the introgression of the B. rapa genome significantly increased the genetic diversity of B. juncea.
HORTICULTURE RESEARCH
(2022)
Article
Plant Sciences
Prashant Vasisth, Naveen Singh, Omkar Maharudra Limbalkar, Mohit Sharma, Gokulan Dhanasekaran, Mohan Lal Meena, Priyanka Jain, Sarika Jaiswal, Mir Asif Iquebal, Anshul Watts, Kiran B. Gaikwad, Rajendra Singh
Summary: Interspecific hybridization has led to the development of improved canola varieties with higher productivity and adaptability. Through the analysis of multiple traits in different hybrids, specific genetic factors contributing to yield have been identified. This research demonstrates the effectiveness of interspecific hybridization in enhancing crop diversity and productivity.
Article
Plant Sciences
Haitian Yu, Kan-Fa Chang, Sheau-Fang Hwang, Stephen E. Strelkov, Paula Baptista, Livy Williams
Summary: Multiple species of Fusarium can cause root rot in canola, and there is a strong correlation between root rot severity at the seedling stage and at maturity. The interaction between Fusarium oxysporum and F. proliferatum leads to more severe root rot. Understanding these interactions and the relative virulence of Fusarium species can improve the management of root rot in canola.
Article
Horticulture
Qun Feng, Jie Yu, Xiaoli Yang, Xianju Lv, Yanmeng Lu, Jiangnan Yuan, Xuye Du, Bin Zhu, Zaiyun Li
Summary: Interspecific hybridization is an important breeding method that enriches genetic resources for crops. The study successfully developed a new octaploid Raphanobrassica interspecific hybrid, which can serve as intermediate germplasm for introducing desirable traits into Brassica crops and as a founder for experimental synthesis of new species.
SCIENTIA HORTICULTURAE
(2022)
Article
Plant Sciences
Elvis Katche, Roman Gaebelein, Zurianti Idris, Paula Vasquez-Teuber, Yu-tzu Lo, David Nugent, Jacqueline Batley, Annaliese S. Mason
Summary: The study investigates the hybridization of Brassica juncea and Brassica carinata (2n = BBAC) over six generations, revealing improved meiotic pairing behavior and fertility levels approaching those of the parent species. The hybrids contain a stable A/C genome formed from recombination of A and C-genome chromosomes. This provides experimental evidence that two genomes can come together to form a new, restructured genome in hybridization events between two allotetraploid species that share a common genome.
Article
Plant Sciences
Shannon F. Greer, Dieter Hackenberg, Vasilis Gegas, Georgia Mitrousia, David Edwards, Jacqueline Batley, Graham R. Teakle, Guy C. Barker, John A. Walsh
Summary: Turnip yellows virus (TuYV) is transmitted by aphids and causes yield losses in oilseed rape and vegetable brassicas. Resistance to TuYV has been identified and mapped in the diploid progenitor species of OSR, Brassica rapa and Brassica oleracea, with QTLs located on chromosomes A02, A06, and C05. The discovery of these new resistance QTLs provides opportunities for developing varieties with enhanced resistance to TuYV.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Omkar Maharudra Limbalkar, Rajendra Singh, Parvesh Kumar, Joghee Nanjundan, Chiter Mal Parihar, Prashant Vasisth, Devendra Kumar Yadava, Viswanathan Chinnusamy, Naveen Singh
Summary: The study aimed to establish hybrid vigor for drought tolerance and water use efficiency by evaluating 105 hybrids developed from intermating 15 CDLs. Traits studied were found to be governed by both additive and non-additive gene actions, with hybrids showing higher productivity under drought conditions. Significant general combining ability (GCA) and specific combining ability (SCA) effects were observed for some CDLs and hybrids, indicating potential for improving yield in drought-prone areas. Water use efficiency (WUE) varied among parental CDLs and hybrids, with hybrids showing higher WUE under both rainfed and irrigated conditions. Selection indices such as drought tolerance index (DTI) and mean relative performance (MRP) were found to be effective in identifying productive CDLs and hybrids under drought conditions.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Miaomiao Liu, Fangdi Hu, Lijun Liu, Xiaoming Lu, Rong Li, Jingyu Wang, Junyan Wu, Li Ma, Yuanyuan Pu, Yan Fang, Gang Yang, Wangtian Wang, Wancang Sun
Summary: Hypocotyl length is an important trait that affects the cold tolerance of Brassica napus. In this study, a segregating population was constructed using two contrasting parental varieties, and BSA-Seq was used to identify candidate genes for hypocotyl length. The results revealed genetic control of hypocotyl length and identified a QTL locus associated with this trait. These findings provide valuable insights into the genetic basis of hypocotyl length in winter rapeseed and have implications for breeding programs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Kawalpreet Kaur, Yingyi Liu, Habibur Rahman
Summary: In this study, clubroot disease resistance was successfully introgressed from turnip to canola through backcrossing and selective breeding, revealing the genetic relationships among different resistance pathotypes.
Article
Plant Sciences
Stefanie Ambros, Mona Kotewitsch, Philipp R. Wittig, Bettina Bammer, Angelika Mustroph
Summary: In this study, the transcriptional response of rapeseed roots to hypoxia was analyzed using RNAseq. The results showed that rapeseed roots had similar responses to hypoxia stress as some hypoxia-induced genes, and there was no difference in response between the two rapeseed cultivars used in the study. However, root hypoxia had minimal effect on the carbohydrate status of the roots. The leaves did not respond to the root stress after a 24-hour treatment.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Etsuko Itabashi, Kenji Osabe, Ryo Fujimoto, Tomohiro Kakizaki
PLANT CELL REPORTS
(2018)
Article
Agronomy
Eigo Fukai, Md. Masud Karim, Daniel J. Shea, Nazmoon Naher Tonu, Kevin C. Falk, Taketo Funaki, Keiichi Okazaki
MOLECULAR BREEDING
(2019)
Article
Multidisciplinary Sciences
Daniel J. Shea, Namiko Nishida, Satoko Takada, Etsuko Itabashi, Satoshi Takahashi, Ayasha Akter, Naomi Miyaji, Kenji Osabe, Hasan Mehraj, Motoki Shimizu, Motoaki Seki, Tomohiro Kakizaki, Keiichi Okazaki, Elizabeth S. Dennis, Ryo Fujimoto
SCIENTIFIC REPORTS
(2019)
Article
Genetics & Heredity
Ayasha Akter, Satoshi Takahashi, Weiwei Deng, Daniel J. Shea, Etsuko Itabashi, Motoki Shimizu, Naomi Miyaji, Kenji Osabe, Namiko Nishida, Yutaka Suzuki, Chris A. Helliwell, Motoaki Seki, William James Peacock, Elizabeth S. Dennis, Ryo Fujimoto
Article
Multidisciplinary Sciences
Satoko Takada, Ayasha Akter, Etsuko Itabashi, Namiko Nishida, Daniel J. Shea, Naomi Miyaji, Hasan Mehraj, Kenji Osabe, Motoki Shimizu, Takeshi Takasaki-Yasuda, Tomohiro Kakizaki, Keiichi Okazaki, Elizabeth S. Dennis, Ryo Fujimoto
SCIENTIFIC REPORTS
(2019)
Article
Plant Sciences
Hasan Mehraj, Takahiro Kawanabe, Motoki Shimizu, Naomi Miyaji, Ayasha Akter, Elizabeth S. Dennis, Ryo Fujimoto
Article
Biochemistry & Molecular Biology
Md Masud Karim, Abdulsalam Dakouri, Yan Zhang, Qilin Chen, Gary Peng, Stephen E. Strelkov, Bruce D. Gossen, Fengqun Yu
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Plant Sciences
Hasan Mehraj, Ayasha Akter, Naomi Miyaji, Junji Miyazaki, Daniel J. Shea, Ryo Fujimoto, Md. Asad-ud Doullah
Article
Plant Sciences
Naomi Miyaji, Motoki Shimizu, Takeshi Takasaki-Yasuda, Elizabeth S. Dennis, Ryo Fujimoto
Summary: Fusarium yellows-resistant and susceptible lines exhibit different responses to salicylic acid (SA), with the resistant line activating SA-responsive genes involved in defense responses. Specific SA-induced genes in resistant lines may play a role in defense against Fusarium oxysporum f. sp. conglutinans. The study highlights the potential importance of SA in disease resistance in Brassica rapa and suggests an antagonistic interaction between SA and jasmonic acid/ethylene responses in Fusarium yellows resistance mechanism.
PLANT CELL REPORTS
(2021)
Article
Multidisciplinary Sciences
Abdulsalam Dakouri, Mebarek Lamara, Md. Masud Karim, Jinghe Wang, Qilin Chen, Bruce D. Gossen, Stephen E. Strelkov, Sheau-Fang Hwang, Gary Peng, Fengqun Yu
Summary: The study identified several Brassica napus accessions with strong resistance to new, virulent pathotypes of Plasmodiophora brassicae. Through genotyping by sequencing and mixed linear model analysis, 13 important SNP loci associated with resistance to different pathotypes were identified.
SCIENTIFIC REPORTS
(2021)
Review
Horticulture
Saaya Shiraki, Kazumasa Fujiwara, Yoshiki Kamiya, Mst. Arjina Akter, Elizabeth S. Dennis, Ryo Fujimoto, Hasan Mehraj
Summary: Heterosis is an important phenomenon in high-yield crop breeding, but its molecular mechanism is not well understood in horticultural crops. This review summarizes the new genetic and epigenetic studies on heterosis in horticultural crops. Molecular approaches are being used to study the molecular basis of heterosis in horticultural crops due to the difficulty of predicting heterosis from parental genetic distance. Transcriptome analyses have identified photosynthesis-related genes and noncoding RNAs as important factors in heterosis. QTL analysis has revealed the association of specific loci with heterosis in vegetative and fruit tissues.
Article
Agronomy
Ayasha Akter, Tomohiro Kakizaki, Etsuko Itabashi, Kohei Kunita, Motoki Shimizu, Mst. Arjina Akter, Hasan Mehraj, Keiichi Okazaki, Elizabeth S. S. Dennis, Ryo Fujimoto
Summary: In this study, the genotyping of 310 Brassica rapa accessions revealed 19 homozygous and 81 heterozygous putative functional alleles of the flowering time gene BrFLC5. Overexpression of BrFLC5 was found to regulate flowering in Arabidopsis thaliana. The expression level of BrFLC5 was not associated with the G/A polymorphism in the third intron, but rather with a 584-bp insertion in the promoter region. The potential impact of high expression of functional BrFLC5 on premature bolting-resistant lines in B. rapa vegetables is discussed.
MOLECULAR BREEDING
(2023)
Article
Horticulture
Mst. Arjina Akter, Hasan Mehraj, Naomi Miyaji, Satoshi Takahashi, Takeshi Takasaki-Yasuda, Motoaki Seki, Elizabeth S. Dennis, Ryo Fujimoto, Kenji Osabe
Summary: This study found that two active histone modifications are associated with the expression level of lncRNAs in Brassica rapa, and they are enriched around the transcription start site. The transcription level of long intergenic noncoding RNAs is positively correlated with the level of histone modifications. Furthermore, the expression levels of mRNAs and their paired NATs are positively correlated after Fusarium oxysporum f. sp. conglutinans inoculation.
Article
Plant Sciences
Fengqun Yu, Yan Zhang, Jinghe Wang, Qilin Chen, Md. Masud Karim, Bruce D. Gossen, Gary Peng
Summary: This study identified resistance loci for different pathotypes of clubroot disease in canola through genomic sequencing and QTL analysis, and discovered some potential genes associated with resistance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Maho Sato, Motoki Shimizu, Daniel J. Shea, Mozammel Hoque, Takahiro Kawanabe, Naomi Miaji, Ryo Fujimoto, Eigo Fukai, Keiichi Okazaki