Review
Chemistry, Multidisciplinary
Fei Wang, Aftab Ullah, Xuelian Fan, Zhou Xu, Rongling Zong, Xuewen Wang, Gang Chen
Summary: An appropriate delivery system can enhance the immune effects of antigens against infections or tumors. Targeting vaccine delivery systems to APCs is a promising strategy for provoking potent immune responses.
JOURNAL OF CONTROLLED RELEASE
(2021)
Review
Immunology
Ger T. Rijkers, Nynke Weterings, Andres Obregon-Henao, Michaela Lepolder, Taru S. Dutt, Frans J. van Overveld, Marcela Henao-Tamayo
Summary: Infection with SARS-CoV-2 leads to the pandemic disease COVID-19, but effective vaccines such as mRNA and viral vector vaccines have been developed to control the spread. These vaccines can induce immune-related side effects like allergic reactions and thrombosis events, especially in rare cases.
Review
Biochemistry & Molecular Biology
Anella Saggese, Loredana Baccigalupi, Giuliana Donadio, Ezio Ricca, Rachele Isticato
Summary: The development of efficient mucosal vaccines relies on appropriate vectors. The bacterial spore has been proposed as a vaccine delivery system, displaying antigens on its surface and inducing specific immune responses in animals. The advantages and drawbacks of using the spore and different approaches to display antigens are discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Farzana Hossain, Shruthi Kandalai, Xiaozhuang Zhou, Nan Zhang, Qingfei Zheng
Summary: In this review, synthetic approaches for antigen-based cancer vaccines are highlighted, along with the discussion of various bioconjugation strategies. The progress of genetically modified cancer vaccines in clinical research is also summarized. Additionally, the synthesis of adjuvants and utilization of biomaterial scaffolds for enhancing immune responses are mentioned.
Article
Multidisciplinary Sciences
Xiaoqing Shi, Jiage Ding, Yanyan Zheng, Jiawe Wang, Navid Sobhani, Praveen Neeli, Gang Wang, Junnian Zheng, Dafei Chai
Summary: PLGA/PEI-HMGB1/GPC3 vaccine induced a strong and long-lasting CTL effect, inhibiting the progression and re-attack of hepatocellular carcinoma.
Article
Nanoscience & Nanotechnology
Haoqian Chen, Xiao Zheng, Lingjue Li, Lishuxin Huang, Weiwei Huang, Yanbing Ma
Summary: This study developed a simple technique for combining a peptide and a delivery system using the natural secretion system of bacteria. The system biosynthesizes an antigenic peptide in bacteria and releases it extracellularly through bacterial secretory vesicles, achieving both peptide biosynthesis and delivery. The system utilizes the natural properties of bacterial vesicles to promote antigen uptake and dendritic cell maturation, inducing tumor-specific CD4+ Th1 and CD8+ CTL responses and effectively suppressing tumor growth in TC-1 tumor-bearing mice. This research promotes innovation and extends the application of peptide-based vaccine biosynthesis technology, providing a new method for personalized cancer immunotherapy using screened peptides as antigens in the future.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Pharmacology & Pharmacy
Yukinobu Kodama, Ayako Tokunaga, Junya Hashizume, Hiroo Nakagawa, Hitomi Harasawa, Tomoaki Kurosaki, Tadahiro Nakamura, Koyo Nishida, Mikiro Nakashima, Mitsuru Hashida, Shigeru Kawakami, Hitoshi Sasaki
Summary: A biocompatible splenic vector for a DNA vaccine against melanoma was developed, showing strong luciferase activity and suppression of melanoma growth and metastasis in mice. The ternary complex mainly transfected into macrophages in the spleen, indicating its suitability for DNA vaccination. The ternary complex also exhibited low acute and liver toxicities in mice, confirming its safety profile.
Review
Oncology
Cathrine Lund Lorentzen, John B. Haanen, Oezcan Met, Inge Marie Svane
Summary: Years of research on mRNA vaccines for cancer treatment have paved the way for the rapid development of mRNA vaccines during the COVID-19 pandemic. mRNA-based cancer vaccines offer potential advantages in terms of tolerability and easy production, making them a promising option for cancer immunotherapy. The latest clinical advances in mRNA-based vaccines for cancer treatment are summarized in this review, along with future perspectives and challenges for this new and promising treatment approach.
Article
Oncology
Carmen Aguilar-Gurrieri, Ana Barajas, Carla Rovirosa, Raquel Ortiz, Victor Urrea, Nuria de la Iglesia, Bonaventura Clotet, Julia Blanco, Jorge Carrillo
Summary: Neoantigens are tumor-specific antigens that can elicit a specific immune response. They have potential for the development of personalized cancer vaccines. The presentation of neoantigens to T cells is influenced by vaccine delivery strategies, including the use of specific linkers. The efficiency of neoantigen processing and presentation by MHC-I molecules can be improved with alanine-based linkers.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2023)
Article
Engineering, Environmental
Junyan Li, Yuanyuan Wu, Jian Xiang, Hairong Wang, Qi Zhuang, Ting Wei, Zhiqin Cao, Qingyang Gu, Zhuang Liu, Rui Peng
Summary: Scientists have synthesized F-PEI nanovaccine for mRNA delivery, which can induce dendritic cell maturation and trigger effective antigen presentation, leading to strong anti-tumor immune responses. This research provides a new tool for mRNA delivery, promising for personalized cancer vaccines as well as other mRNA-based immunotherapies.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Multidisciplinary Sciences
Lei Lei, Dennis Huang, Huile Gao, Bin He, Jun Cao, Nicholas A. Peppas
Summary: This review introduces the advantages of using hydrogels in cancer vaccine delivery systems, including their ability to encapsulate antigens and protect them from degradation, reverse immunosuppression and stimulate immune response, as well as their precise drug release capability.
Review
Nanoscience & Nanotechnology
Marina A. Dobrovoskaia, Mark Bathe
Summary: Gene therapeutics show promise in treating genetic diseases and cancer through modulation of gene expression and immune cell activation, but face limitations in toxicity, targeting, and efficiency compared to viral vectors. Structured DNA assemblies fabricated using DNA origami principles offer a new nonviral delivery vector with controllable immunostimulatory properties and low manufacturing cost.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2021)
Review
Multidisciplinary Sciences
Lei Lei, Dennis Huang, Huile Gao, Bin He, Jun Cao, Nicholas A. Peppas
Summary: This review summarizes the classification of cancer vaccines, highlights the strategies of hydrogel-based cancer vaccines, and provides some insights into the future development of hydrogel-based cancer vaccines.
Review
Pharmacology & Pharmacy
Abdellatif Bouazzaoui, Ahmed A. H. Abdellatif, Faisal A. Al-Allaf, Neda M. Bogari, Saied Al-Dehlawi, Sameer H. Qari
Summary: The current COVID-19 pandemic has prompted a global effort to develop effective vaccines against the SARS-CoV-2 virus, which has caused significant economic, social, and psychological concerns. Research teams worldwide are adopting various strategies, from conventional approaches to novel methods, to combat this virulent pathogen and address the challenges in vaccine development.
Review
Immunology
Samantha J. Paston, Victoria A. Brentville, Peter Symonds, Lindy G. Durrant
Summary: Vaccination has had a significant impact in preventing infectious diseases, but the development of therapeutic cancer vaccines has been challenging.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemical Research Methods
Leandro J. Benedini, Douglas Figueiredo, Joaquin Cabrera-Crespo, Viviane M. Goncalves, Gabriel G. Silva, Gilson Campani, Teresa C. Zangirolami, Felipe F. Furlan
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Biotechnology & Applied Microbiology
Thais S. Milessi, Caroline L. Perez, Teresa C. Zangirolami, Felipe A. S. Corradini, Juliana P. Sandri, Maria R. Foulquie-Moreno, Roberto C. Giordano, Johan M. Thevelein, Raquel L. C. Giordano
BIOTECHNOLOGY FOR BIOFUELS
(2020)
Article
Materials Science, Paper & Wood
Thais S. Milessi, Claudia R. Silva, Guilherme S. Moraes, Patricia M. Aquino, Roberto C. Giordano, Raquel L. C. Giordano, Teresa C. Zangirolami
Article
Multidisciplinary Sciences
Fabiana Lauretti-Ferreira, Paloma L. D. Silva, Naiara M. Alcantara, Bruna F. Silva, Isabele Grabher, Gisele O. Souza, Erika Nakajima, Milena A. Akamatsul, Silvio A. Vasconcellos, Patricia A. E. Abreu, Eneas Carvalho, Elizabeth A. L. Martine, Paulo L. Ho, Josefa B. da Silva
Article
Biotechnology & Applied Microbiology
Felipe A. S. Corradini, Thais S. Milessi, Viviane M. Goncalves, Roberto Ruller, Cintia R. Sargo, Laiane A. Lopes, Teresa C. Zangirolami, Paulo W. Tardioli, Roberto C. Giordano, Raquel L. C. Giordano
Summary: In this study, a recombinant beta-xylosidase from Bacillus subtilis was immobilized on agarose and chitosan, showing high thermal stability and improved enzyme kinetic behavior. The immobilized enzymes remained efficient in corncob xylan hydrolysis after multiple cycles, indicating their potential as biocatalysts in a biorefinery context.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Vinicius Marquioni, Francis Morais Franco Nunes, Maria Teresa Marques Novo-Mansur
Summary: Mass spectrometry is crucial in proteomics, and database searching plays a significant role in protein identification. While many authors suggest teaching mass spectrometry to undergraduate students, the importance of database searching is often overlooked. Therefore, our proposal for a class specifically focusing on database searching of mass spectrometry data aims to improve students' understanding of protein identification by mass spectrometry.
JOURNAL OF CHEMICAL EDUCATION
(2021)
Article
Multidisciplinary Sciences
Flavia S. Zandonadi, Silvia P. Ferreira, Andre V. Alexandrino, Carolina M. Carnielli, Juliana Artier, Mariana P. Barcelos, Nicole C. S. Nicolela, Evandro L. Prieto, Leandro S. Goto, Jose Belasque, Maria Teresa Marques Novo-Mansur
Article
Biotechnology & Applied Microbiology
Thiago J. B. Mesquita, Gilson Campani, Roberto C. Giordano, Teresa C. Zangirolami, Antonio C. L. Horta
Summary: A machine learning control strategy, based on an artificial neural network, was proposed to enhance the productivity and yield of micro-aerobic fermentation by controlling oxygen uptake rate. Compared to traditional fermentation methods and other micro-aeration strategies, this approach demonstrated superior performance in experimental settings, improving ethanol productivity and yield.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Adilson Jose da Silva, Josivan de Souza Cunha, Teri Hreha, Kelli Cristina Micocci, Heloisa Sobreiro Selistre-de-Araujo, Blanca Barquera, Mattheos A. G. Koffas
Summary: This study engineered the pyocyanin biosynthetic pathway for high production in a heterologous host for the first time. Optimized culture and induction parameters led to a 3.5-fold increase in pyocyanin accumulation, while pathway balancing through testing plasmids with different copy numbers resulted in a fourfold difference in product titer among the engineered strains. Co-expression of Vitreoscilla hemoglobin Vhb further improved pyocyanin production to a final titer of 18.8 mg/L, demonstrating the potential of using Escherichia coli for phenazines production in electro-fermentation systems.
METABOLIC ENGINEERING
(2021)
Article
Microbiology
Camila L. Campos, Luciana R. Gomes, Ambart E. Covarrubias, Ellen E. Kato, Gisele G. Souza, Silvio A. Vasconcellos, Marcos B. Heinemann, Elizabeth A. L. Martins, Paulo L. Ho, Renata M. A. Da Costa, Josefa B. Da Silva
Summary: Leptospirosis is a global zoonotic disease, and a three-dimensional human lung cell culture model has been established to study the infection mechanism and interaction with the host for this disease.
CURRENT MICROBIOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Caroline L. Perez, Lais P. R. da C. Pereira, Thais S. Milessi, Juliana P. Sandri, Mekonnen Demeke, Maria R. Foulquie-Moreno, Johan M. Thevelein, Teresa C. Zangirolami
Summary: A strategy based on strain evaluation and integration with 1G ethanol production was used to develop a robust and scalable process for 2G ethanol production. The study found that using mixed medium of crude bagasse hydrolysate and molasses achieved high ethanol concentrations and long-term stability.
Article
Multidisciplinary Sciences
Vinicius Marquioni, Fernando Pacheco Nobre Rossi, Deborah Cezar Mendonca, Layla Farage Martins, Franklin Behlau, Joao Carlos Setubal, Aline Maria da Silva, Maria Teresa Marques Novo-Mansur
Summary: Citrus canker is a bacterial disease that affects citrus crops worldwide. This study reports the isolation and characterization of a new jumbo phage, LucasX, which can infect Xanthomonas citri and X. fuscans. LucasX has a large genome and shows potential as a biocontrol agent for citrus canker.
Article
Chemistry, Medicinal
Gabriel Pinna Feliciano, Sara Hayama Arimori, Vinicius Watanabe Nakao, Joice Rodrigues Dos Santos, Elizabeth A. L. Martins, Marcelo Porto Bemquerer, Elisabeth Cheng
Summary: This study successfully achieved the separation of FVIII and PCC by directly loading plasma onto anion exchange resin under high ionic strength. Using certain resins, blood products can be purified quickly without the need for cryoprecipitation.
Article
Education, Scientific Disciplines
Gabriel Luz Chaves, Raquel Salgado Batista, Josivan de Sousa Cunha, Daniel Lossa Altmann, Adilson Jose da Silva
Summary: This article introduces the use of simulation tool OptFlux to teach cellular metabolism to chemical engineers. Students explore the quantitative aspects of cellular metabolism by simulating the behavior of Escherichia coli and Saccharomyces cerevisiae cells under different environmental conditions. The students' feedback regarding the use of OptFlux as an educational tool is highly positive, with most students finding it a useful teaching strategy.
EDUCATION FOR CHEMICAL ENGINEERS
(2022)
Article
Engineering, Environmental
Jussara F. Carneiro, Jose M. Aquino, Bianca F. Silva, Adilson J. Silva, Romeu C. Rocha-Filho
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2020)