Article
Spectroscopy
Jianling Chen, Chunliu Yang, Hailiang Nie, Haiyin Li
Summary: Development of high-performance fluorescence sensors for pesticide has been a challenging task. Most existing fluorescence sensors use enzyme-inhibited strategy, which is expensive and prone to interference. Aptamer-based fluorescence sensors require intricate processes and expensive materials. In this study, a novel aptamer-based fluorescence system is developed for label-free, enzyme-free, and highly sensitive detection of profenofos pesticide. The system utilizes hybridization chain reaction (HCR)-assisted signal amplification and specific intercalation of N-methylmesoporphyrin IX (NMM) in G-quadruplex DNA to achieve improved detection performance.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Food Science & Technology
Wanling Cui, Guodong Hu, Enguang Lv, Chonghui Li, Zhenxing Wang, Qiang Li, Zhaohong Qian, Jihua Wang, Shicai Xu, Rui Wang
Summary: A label-free and enzyme-free fluorescent aptasensor was developed for quantifying kanamycin in milk, with high sensitivity and selectivity. It has the potential for wide application in ensuring food safety.
Article
Chemistry, Analytical
Junhyun Park, Hyogu Han, Chihyun Park, Jun Ki Ahn
Summary: In this study, a personal glucose pyrophosphatase (PPase) detection method is demonstrated using cascade enzymatic reaction (CER) promoted by hexokinase and pyruvate kinase. Compared with previous methods, this method eliminates the need for expensive equipment and complex procedures, and allows quantitative evaluation of PPase activity within a dynamic linear range.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Wenjing Wang, Fei Zhai, Fupei Xu, Min Jia
Summary: Novel enzyme-free amplified dual-terminal labeled split aptamer (SPA) probes were developed for one-step rapid and sensitive detection of bisphenol A (BPA). These probes showed a low detection limit and rapid response, making them suitable for onsite detection.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Funa Meng, Zhongxi Jiang, Yajing Li, Peina Zhang, Haisheng Liu, Yanli Sun, Xueliang Wang
Summary: A enzyme-free and label-free electrochemical sensor was developed for detecting PNK activity. The sensor utilized the catalytic characteristic of PNK on DNA phosphorylation to achieve detection. The method showed good linear correlation and high sensitivity, making it a potential tool for PNK concentration detection in biological research and clinical diagnosis.
Article
Chemistry, Analytical
Wenjiao Zhou, Lin Xu, Bingying Jiang
Summary: A label-free and highly sensitive fluorescent aptasensing platform was developed for detecting kanamycin in milk samples. This method demonstrated good selectivity, low detection limit, and the potential for sensitive monitoring of trace amounts of kanamycin for food safety applications.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Ping Zhou, Yingbo Pan, Wei Pan, Sumei Lu, Jiaqi Yin, Na Li, Bo Tang
Summary: A duplex-specific nuclease (DSN)-mediated and dual-AND logic gate-based triple-line lateral flow strip detection system was developed for the rapid and simultaneous detection of four lung cancer-related miRNAs. This system combines DSN-mediated signal amplification with AND logic gate-based simple signal output, and has a detection limit of 26.51 fM. Additionally, this assay can detect lung cancer-related miRNAs from serum in a homogeneous and separation-free format, with an accuracy of 100%. This approach provides a simple and easy-to-handle method for the diagnosis of lung cancer in clinic.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Qian Zhao, Guangyin Zhang, Dai Lu, Kejun Feng, Xingbo Shi
Summary: A highly sensitive aptasensor based on dual-enzyme mediated cascade-signal amplification was developed for the detection of ampicillin. The sensor exhibited excellent detection performance and versatility, paving the way for efficient sensors for detecting other trace food hazards.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Inorganic & Nuclear
Salim Ali, Suranjan Sikdar, Shatarupa Basak, Md Salman Haydar, Kangkan Mallick, Modhusudan Mondal, Debadrita Roy, Shibaji Ghosh, Sanjay Sahu, Paramita Paul, Mahendra Nath Roy
Summary: A biosensor with crystalline CuS nanoparticles was synthesized without a surfactant. The CuS nanoparticles had a hexagonal crystalline structure and spherical shape. This study focused on the peroxidase-mimicking activity, superoxide dismutase-mimicking activity, and colorimetric determination of epinephrine neurotransmitters.
INORGANIC CHEMISTRY
(2023)
Article
Chemistry, Analytical
Cheng-Yu Lee, Chi-Hsiang Liao, Nei-Mei Fang, You-Zung Hsieh
Summary: A DNAzyme-based biosensor was developed for the detection of PPase activity, offering high sensitivity, a wide linear range, and single-step operation.
Article
Chemistry, Multidisciplinary
William Etheridge, Frederic Brossard, Sitan Zheng, Svenja Moench, Suraj Pavagada, Roisin M. Owens, Ljiljana Fruk
Summary: By using polydopamine and gold nanoparticles, the reaction rate of copper-selective DNA cleaving DNAzyme can be significantly enhanced, making the combination of polydopamine and DNAzyme suitable for practical applications as a sensitive biosensor for Cu(ii) ions. Deposition of DNAzyme onto a gold electrode followed by Polydopamine Assisted DNA Immobilisation (PADI) allows for the development of a cost-effective, label-free, and fast electrochemical biosensor with a low limit of detection, thus paving the way for a new generation of hybrid DNAzyme-based biosensors.
Article
Engineering, Environmental
Yong Zhang, Chengyong Wu, Hongxin Liu, Mohammad Rizwan Khan, Zhifeng Zhao, Guiping He, Aimin Luo, Jiaqi Zhang, Ruijie Deng, Qiang He
Summary: This study presents a label-free, dually amplified and homogeneous DNAzyme assay for sensitive and one-pot detection of lead pollution, which can precisely detect Pb2+ in tap water, milk and fish, promising for on-site monitoring lead pollution risk and ensuring environmental and food safety.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Fei Ma, Ya-Zhen Liu, Meng Liu, Jian-Ge Qiu, Chun-Yang Zhang
Summary: For the first time, fluorogenic RNA aptamers have been utilized for label-free uracil-DNA glycosylase (UDG) assay. By engineering the transcription machine with dU substitution, this assay only requires a single probe to simultaneously sense and amplify the UDG signal, achieving a low detection limit. Moreover, it can be used for screening UDG inhibitors and measuring endogenous UDG activity in different cells.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Dexin Du, Jue Wang, Mingquan Guo, Jiangnan Shu, Wei Nie, Zhiping Bian, Di Yang, Hua Cui
Summary: Label-free electrochemiluminescence (ECL) immunoassays (lf-ECLIA) have become an attractive method for biomarker detection due to their simplicity, speed, and cost-effectiveness. This study investigated the interaction between biomarkers and analytical interfaces constructed using a nanoluminophore and antibody-functionalized gold nanoparticles. The results showed that positively charged biomarkers increased the ECL intensity, while negatively charged biomarkers decreased the ECL intensity. A charge-dependent lf-ECLIA method was established based on the ECL signal changes, which exhibited different responses to positively charged and negatively charged biomarkers.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Qian Ma, Sam Fong Yau Li
Summary: This study presents a novel method for quantitative detection of miRNA, utilizing the distance-dependent property of photoinduced electron transfer between G-quadruplex/hemin complexes and DNA/Cu nano particles. The method enables the sensitive and specific determination of miRNA let-7a with a relatively low limit of detection, and has been successfully applied for enzymeand label-free fluorescence detection of miRNA from real samples.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Yung-Peng Wu, Chee Ying Chew, Tian-Neng Li, Tzu-Hsuan Chung, En-Hao Chang, Chak Hin Lam, Kui-Thong Tan
Article
Chemistry, Analytical
Yu-Hsuan Chen, Wan-Ching Chien, Di-Chi Lee, Kui-Thong Tan
ANALYTICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Pei-Jhen Li, Mohammad Tarigue Anwar, Chen-Yo Fan, Duane S. Juang, Hsin-Yi Lin, Tsung-Che Chang, Sachin Kisan Kawade, Hsiang-Jung Chen, Yu-Ju Chen, Kui-Thong Tan, Chun-Cheng Lin
Article
Chemistry, Organic
Jih Ru Hwu, Animesh Roy, Avijit Panja, Wen-Chieh Huang, Yu-Chen Hu, Kui-Thong Tan, Chun-Cheng Lin, Kuo-Chu Hwang, Ming-Hua Hsu, Shwu-Chen Tsay
JOURNAL OF ORGANIC CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Hsin-Ju Chan, Xin-Hui Lin, Syuan-Yun Fan, Jih Ru Hwu, Kui-Thong Tan
CHEMISTRY-AN ASIAN JOURNAL
(2020)
Article
Chemistry, Analytical
Yu-Hsuan Chen, Nitesh K. Gupta, Hsiao-Jung Huang, Chak Hin Lam, Ching-Lan Huang, Kui-Thong Tan
Summary: The lateral flow assay (LFA) has been an important diagnostic tool, with the recent development of affinity-switchable lateral flow assay (ASLFA) overcoming limitations of traditional LFAs, enabling rapid detection of molecules that were previously undetectable by conventional methods.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Hsiao-Jung Huang, Yu-Ting Lin, Min-Chi Chung, Yu-Hsuan Chen, Kui-Thong Tan
Summary: This paper reports an affinity-switchable lateral flow assay (ASLFA) for rapid and selective detection of hydrogen peroxide, glucose, and ethanol in blood serum and urine samples. The ASLFA approach, based on a gold nanoparticle-conjugated ASbiotin probe, can be an alternative to classical detection methods where simple and rapid on-site detection is essential.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
You-Ru Cai, Yi-Ting Liu, Xue-Feng Luo, Yin-Yu Lee, Kui-Thong Tan, I-Chia Chen
Summary: In this study, the metal-enhanced fluorescence (MEF) effect of rhodamine dye by gold sub-micro hexagonal plates was investigated. A multilayered avidin-biotin complex was used to connect the fluorophore and the sub-microplate. Fluorescence lifetime image microscopy (FLIM) combined with time-correlated single-photon counting was employed to obtain lifetime images and intensities of dye on single particles. The results showed that the number of layers of gold sub-microplates affected the emission intensity and lifetime of the dye.
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Fang-Ling Lin, Jui-Ting Yen, Pin-Wen Fang, Shun-Qiang Xu, Jing-Cyun Lin, Kui-Thong Tan
Summary: This paper describes a new strategy using an affinity-based protein-labeling fluorescent probe to study ectodomain shedding. The study identifies the ADAM17 metalloprotease as responsible for the shedding of carbonic anhydrases and shows that this protein-labeling approach provides a more detailed insight into the mechanism of ectodomain shedding and protein lifetime on the cell surface, compared to current immunological methods.
ACS CHEMICAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Chien-Chi Wu, Shao-Jie Huang, Tsung-Yu Fu, Fang-Ling Lin, Xin-You Wang, Kui-Thong Tan
Summary: This paper introduces a new affinity-tunable protein switch strategy that can activate specific protein binding events and generate fluorescent signals by changing the protein's conformation. This modular sensing concept can selectively detect different drugs on the cell surface.
Article
Chemistry, Multidisciplinary
Ping-Chien Wu, Kui-Thong Tan, I-Chia Chen
Summary: The trans-cis isomerization of linear merocyanine L-Mero4 and phenyl substituted linear merocyanine P-L-Mero4 in salt solution and ionic liquid was studied using frequency upconversion measurements. The addition of strontium chloride and cesium iodide to the solvents dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) allowed for variation of the ionic strength. The results showed that the interaction between SrCl2 and L-Mero4 was stronger than with P-L-Mero4.
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
(2023)
Article
Chemistry, Analytical
Shun-Qiang Xu, Zong-Yan Sie, Jung- Hsu, Kui-Thong Tan
Summary: In this paper, a small but highly specific PM fluorescent dye, PM-1, is reported, which exhibits a very long retention time on the plasma membrane. The dye can be used to study the dynamic processes of cell surface proteins, such as ectodomain shedding and endocytosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Min-Chi Chung, Yun-Qiao Liu, Bo-Lin Jian, Shun-Qiang Xu, Jhih-Jie Syu, Chin-Fa Lee, Kui-Thong Tan
Summary: In this study, a Signal-ON small molecule competitive assay based on the sterically modulated affinity-switchable interaction of biotin and streptavidin was reported. The steric hindrance on biotin is removed in the presence of the small molecule target, allowing the generation of the desired test line. This affinity-switchable approach could be applied in drug discovery and clinical diagnosis for the rapid analysis of small molecules.
Article
Nanoscience & Nanotechnology
Chen-Yo Fan, Sachin Kisan Kawade, Avijit K. Adak, Chien Cho, Kui-Thong Tan, Chun-Cheng Lin
Summary: The fabrication of Ag-coated cuprous oxide (Cu2O) NP slides promotes metal-enhanced fluorescence effect and enables external copper ion-free CuAAC reaction, providing a practical and alternative sensing platform for biological or diagnostic applications; the immobilization of antibodies on these newly developed slide surfaces using a specific pathway shows better sensitivity compared to the random antibody immobilization method.
ACS APPLIED NANO MATERIALS
(2021)
Article
Chemistry, Analytical
Hsiang-Yun Huang, Syuan-Yun Fan, En-Hao Chang, Chak Hin Lam, Yu-Chun Lin, Xin-Hui Lin, Nitesh K. Gupta, Kui-Thong Tan
ANALYTICAL CHEMISTRY
(2020)