Article
Immunology
Mary A. Brown, Sophie B. Morgan, Gillian E. Donachie, Katie L. Horton, Ian D. Pavord, Carolina V. Arancibia-Carcamo, Timothy S. C. Hinks
Summary: Type-2 low asthma or COPD is potentially caused by persistent bacterial colonization of the lower airways by bacteria such as non-encapsulated Haemophilus influenzae (NTHi), which is a commensal of the upper airways. The extent of NTHi invasion and activation of proinflammatory cytokines in airway epithelial cells is not fully understood. This study found that NTHi can be internalized transiently by airway epithelial cells and induce inflammation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Microbiology
Bryn Short, Stephen Carson, Anna-Claire Devlin, James A. Reihill, Anne Crilly, William MacKay, Gordon Ramage, Craig Williams, Fionnuala T. Lundy, Lorcan P. McGarvey, Keith D. Thornbury, S. Lorraine Martin
Summary: Haemophilus influenzae is a common cause of bacterial infection in the lungs of COPD patients, leading to acute exacerbations. Its ability to adhere to host epithelial cells, form biofilms, and persist through immune evasion and antibiotic tolerance plays a crucial role in the pathogenesis of COPD.
CRITICAL REVIEWS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Lewis J. Williams, Stephen G. Tristram, Graeme R. Zosky
Summary: Exposure to geogenic particulate matter (PM) is associated with increased prevalence of respiratory infections in Australian Indigenous communities. The concentration of iron in geogenic PM is linked to its respiratory health effects, and haematite has the greatest impact on the invasiveness of bronchial epithelial cells by non-typeable Haemophilus influenzae (NTHi).
ENVIRONMENTAL RESEARCH
(2023)
Review
Immunology
Jake R. Weeks, Karl J. Staples, C. Mirella Spalluto, Alastair Watson, Tom M. A. Wilkinson
Summary: Non-typeable Haemophilus influenzae (NTHi) is a common pathogen in COPD patients, but its impact varies among different cohorts. Other bacteria may also contribute to acute exacerbations in COPD. The biofilm phase of NTHi could play a significant role in COPD, exhibiting extreme antimicrobial tolerance and synergistic interactions with other lung pathogens.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Rabeb Dhouib, Marufa Nasreen, Dk Seti Maimonah Pg Othman, Daniel Ellis, Simon Lee, Ama-Tawiah Essilfie, Philip M. Hansbro, Alastair G. McEwan, Ulrike Kappler
Summary: Molybdenum-containing enzyme DmsABC plays a crucial role in infection by H. influenzae, affecting intracellular survival and interactions with the host.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Medicine, Research & Experimental
Shengsen Chen, Xinyun Zhang, Cheng Yang, Shi Wang, Hao Shen
Summary: This study established a mouse model of bacterium-induced acute exacerbation of idiopathic pulmonary fibrosis (AE-IPF) and found that the IL-17 gene is essential for the development of AE-IPF.
Article
Immunology
Xiao Wu, Runfeng Li, Yunceng Weng, Hongxia Zhou, Haiming Jiang, Jin Zhao, Bin Liu, Ruifeng Chen, Xinxin Chen, Weimin Yang, Zifeng Yang, Xinhua Wang
Summary: Primary influenza virus infection can predispose hosts to secondary infection with Haemophilus influenzae, which increases disease severity and mortality. Sequential infection with influenza A virus and Haemophilus influenzae induced lethal synergy in a mouse model, possibly due to increased bacterial loads and lung damage. Analysis showed a correlation between specific adhesion molecules and bacterial growth in secondary pneumonia following primary viral infection.
MICROBES AND INFECTION
(2021)
Article
Infectious Diseases
Shuxian Wen, Ying Mai, Xu Chen, Kun Xiao, Yongping Lin, Zhenbo Xu, Ling Yang
Summary: This study investigates the molecular epidemiology and antibiotic resistance of Haemophilus influenzae in Guangzhou, China. A total of 80 H.influenzae isolates were collected from patients in a hospital from January 2020 to April 2021. The majority of H. influenzae strains were found to be non-typeable H. influenzae, and they showed relative susceptibility to certain antibiotics. The genotyping results revealed high genetic diversity among the isolates.
Article
Microbiology
Ying Zhang, Hongbo Wang, Yanqiu Zhang, Peiliang Zhao, Yanan Li
Summary: Non-typeable Haemophilus influenzae (NTHi) outer membrane vesicles (OMVs) may play a role in the pathogenesis of neutrophilic asthma. It was found that NTHi OMVs induced inflammatory responses and immune-related signaling pathways, resulting in neutrophilic asthma.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Lukas Hleba, Miroslava Hlebova, Eva Kovacikova, Anton Kovacik
Summary: The rapid determination of beta-lactamase presence in H. influenzae isolates was achieved through indirect detection of degradation ampicillin products using MALDI-TOF MS. This method can distinguish resistant and susceptible strains and accelerate the identification of beta-lactamase-producing strains in clinical microbiology.
Article
Infectious Diseases
Young June Choe, Mi Seon Han, Youn Young Choi, Young Joo Sohn, Ye Kyung Kim, Kyung Min Kim, Ji Young Park, Hyun Mi Kang, In Ae Yoon, Jae Hong Choi, Chi Eun Oh, Eun Young Cho, Hyunju Lee, Eun Hwa Choi
Summary: The study in Korea between 2014 and 2019 found that the carriage rates of Streptococcus pneumoniae and non-typeable Haemophilus influenzae in children varied, with a significant increase in carriage of serotype 23A of S. pneumoniae. Children who had received pneumococcal conjugate vaccines had a lower risk of vaccine-type carriage. Continuous surveillance is necessary to evaluate the long-term effects of PCVs on the carriage dynamics of these bacteria.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Immunology
Sara E. Oliver, Amy B. Rubis, Heidi M. Soeters, Arthur Reingold, Meghan Barnes, Susan Petit, Monica M. Farley, Lee H. Harrison, Kathy Como-Sabetti, Sarah A. Khanlian, Rachel Wester, Ann Thomas, William Schaffner, Henju Marjuki, Xin Wang, Susan Hariri
Summary: This study evaluated the epidemiology of NTHi disease in the US, including among pregnant women, infants, and PWH. Results showed that NTHi causes substantial invasive disease, especially among older adults, pregnant/postpartum women, and neonates. Enhanced surveillance and targeted interventions are needed to prevent perinatal NTHi infections.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Microbiology
Pei-Yi Su, Wei-Hung Cheng, Cheng-Hsun Ho
Summary: This study investigated the molecular basis of multidrug-resistant (MDR) non-typeable Haemophilus influenzae (NTHi) and found that genetic variations and plasmid-encoded genes play a significant role in antibiotic resistance. The study also revealed high levels of resistance to various antibiotics among MDR NTHi isolates, highlighting the importance of monitoring drug resistance trends in NTHi.
Article
Multidisciplinary Sciences
Magdiel Perez-Cruz, Bachirou Kone, Remi Porte, Christophe Carnoy, Julien Tabareau, Pierre Gosset, Francois Trottein, Jean-Claude Sirard, Muriel Pichavant, Philippe Gosset
Summary: This study demonstrates that stimulating innate immunity with a TLR5 ligand is an effective antibacterial treatment in COPD-exposed mice, suggesting innovative therapeutic strategies against acute exacerbations in COPD.
Review
Infectious Diseases
Jiying Xiao, Lin Su, Shumin Huang, Lingyue Liu, Kamran Ali, Zhimin Chen
Summary: Haemophilus influenzae (H. influenzae) is a significant pathogen causing respiratory tract infections and invasive diseases, leading to a considerable disease burden. The Hib conjugate vaccine has decreased severe infections caused by Hib strains, but other NTHi serotypes have emerged worldwide. Understanding the epidemic trends and antibiotic resistance characteristics of H. influenzae is important in developing prevention strategies.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Chemistry, Analytical
Birgit Schilling, Brendan MacLean, Jason M. Held, Alexandria K. Sahu, Matthew J. Rardin, Dylan J. Sorensen, Theodore Peters, Alan J. Wolfe, Christie L. Hunter, Michael J. MacCoss, Bradford W. Gibson
ANALYTICAL CHEMISTRY
(2015)
Article
Biochemical Research Methods
Susan E. Abbatiello, Birgit Schilling, D. R. Mani, Lisa J. Zimmerman, Steven C. Hall, Brendan Maclean, Matthew Albertolle, Simon Allen, Michael Burgess, Michael P. Cusack, Mousumi Gosh, Victoria Hedrick, Jason M. Held, H. Dorota Inerowicz, Angela Jackson, Hasmik Keshishian, Christopher R. Kinsinger, John Lyssand, Lee Makowski, Mehdi Mesri, Henry Rodriguez, Paul Rudnick, Pawel Sadowski, Nell Sedransk, Kent Shaddox, Stephen J. Skates, Eric Kuhn, Derek Smith, Jeffery R. Whiteaker, Corbin Whitwell, Shucha Zhang, Christoph H. Borchers, Susan J. Fisher, Bradford W. Gibson, Daniel C. Liebler, Michael J. MacCoss, Thomas A. Neubert, Amanda G. Paulovich, Fred E. Regnier, Paul Tempst, Steven A. Carr
MOLECULAR & CELLULAR PROTEOMICS
(2015)
Article
Biochemical Research Methods
Matthew J. Rardin, Birgit Schilling, Lin-Yang Cheng, Brendan X. MacLean, Dylan J. Sorensen, Alexandria K. Sahu, Michael J. MacCoss, Olga Vitek, Bradford W. Gibson
MOLECULAR & CELLULAR PROTEOMICS
(2015)
Article
Biochemistry & Molecular Biology
Yuya Nishida, Matthew J. Rardin, Chris Carrico, Wenjuan He, Alexandria K. Sahu, Philipp Gut, Rami Najjar, Mark Fitch, Marc Hellerstein, Bradford W. Gibson, Eric Verdin
Article
Biochemistry & Molecular Biology
Birgit Schilling, David Christensen, Robert Davis, Alexandria K. Sahu, Linda I. Hu, Arti Walker-Peddakotla, Dylan J. Sorensen, Bozena Zemaitaitis, Bradford W. Gibson, Alan J. Wolfe
MOLECULAR MICROBIOLOGY
(2015)
Article
Biochemical Research Methods
Jesse G. Meyer, Alexandria K. D'Souza, Dylan J. Sorensen, Matthew J. Rardin, Alan J. Wolfe, Bradford W. Gibson, Birgit Schilling
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2016)
Article
Genetics & Heredity
Pedro Reis Rodrigues, Tiffany K. Kaul, Jo-Hao Ho, Mark Lucanic, Kristopher Burkewitz, William B. Mair, Jason M. Held, Laura M. Bohn, Matthew S. Gill
G3-GENES GENOMES GENETICS
(2016)
Article
Biochemical Research Methods
Anna M. Zawadzka, Birgit Schilling, Michael P. Cusack, Alexandria K. Sahu, Penelope Drake, Susan J. Fisher, Christopher C. Benz, Bradford W. Gibson
MOLECULAR & CELLULAR PROTEOMICS
(2014)
Article
Microbiology
Alaa AbouElfetouh, Misty L. Kuhn, Linda I. Hu, Michael D. Scholle, Dylan J. Sorensen, Alexandria K. Sahu, Doerte Becher, Haike Antelmann, Milan Mrksich, Wayne F. Anderson, Bradford W. Gibson, Birgit Schilling, Alan J. Wolfe
Article
Biochemistry & Molecular Biology
Jessica B. Behring, Sjoerd van der Post, Arshag D. Mooradian, Matthew J. Egan, Maxwell I. Zimmerman, Jenna L. Clements, Gregory R. Bowman, Jason M. Held
Article
Biochemical Research Methods
Arshag D. Mooradian, Sjoerd van der Post, Kristen M. Naegle, Jason M. Held
PLOS COMPUTATIONAL BIOLOGY
(2020)
Article
Cell Biology
Caitlyn B. Brashears, Meltem Barlin, William R. Ehrhardt, Richa Rathore, Matthew Schultze, Shin-Chen Tzeng, Brian A. Van Tine, Jason M. Held
CELL DEATH & DISEASE
(2020)
Article
Biochemical Research Methods
Robert W. Seymour, Sjoerd van Der Post, Arshag D. Mooradian, Jason M. Held
Summary: ProteoSushi is a software tool tailored for analysis of peptide-centric proteomic data sets, facilitating quantitation by summing or averaging intensities for each modified site, merging overlapping peptides charges, and annotating each PTM site with the most up-to-date biological information available.
JOURNAL OF PROTEOME RESEARCH
(2021)