Article
Food Science & Technology
Fuat Aydin, Tuba Kayman, Secil Abay, Harun Hizlisoy, Izzet Burcin Saticioglu, Emre Karakaya, Orhan Sahin
Summary: This study investigated the genetic diversity, prevalence, and mechanisms of resistance to quinolone antibiotics in 178 Campylobacter jejuni isolates from humans, cattle, dogs, and chickens in Turkey. The results showed that there were 21 clonal complexes (CCs) and 78 different sequence types (STs) among the isolates, with CC21, CC353, CC206, and CC257 being the predominant clones. Quinolone resistance was detected in 41% of the isolates, and all resistant isolates had a specific mutation in the gyrA gene. The study provided important insights into the transmission pathways of C. jejuni in Turkey, suggesting that broiler meat and dogs may be the most important sources of human campylobacteriosis in the country.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Xiaofang Wu, Lei Ji, Yuehua Shen, Liping Chen, Deshun Xu, Fenfen Dong
Summary: Thirteen pathogenic Campylobacter strains were isolated from diarrhea cases and raw poultry meat products in Huzhou, including 11 Campylobacter jejuni and 2 Campylobacter coli strains. These strains contain multiple virulence-related genes and show high resistance to nalidixic acid, ciprofloxacin, and tetracycline. Genomic sequencing revealed the presence of tet(O) and blaOXA resistance genes in all C. jejuni strains and multiple drug resistance genes in 2 C. coli strains.
Article
Microbiology
Mehmet Cemal Adiguzel, Debora Brito Goulart, Zuowei Wu, Jinji Pang, Seyda Cengiz, Qijing Zhang, Orhan Sahin
Summary: The study found that FQ-resistant Campylobacter strains mostly carry mutations in the gyrA gene and contain various spacer sequences within the CRISPR system, with many showing high nucleotide homology to known Campylobacter phages. While most spacer sequences matched known phages, there were also some unidentified spacer sequences present.
Article
Microbiology
Xiaoqi Zang, Hongyue Lv, Haiyan Tang, Xinan Jiao, Jinlin Huang
Summary: This study analyzed 1609 Campylobacter jejuni isolates from various ecological origins in China. The results showed that disease associated capsular genotypes and LOS classes over-represented in human isolates were also dominant in animals, especially cattle. High-risk lineages CC-22, CC-464, and CC-21, found in human isolates with GBS worldwide, were also present in food animal isolates with disease associated capsular types, indicating a potential clonal spread across different regions and hosts.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Cecilia Johansson, Christian Kampmann, Anna Nilsson, Johan Dicksved, Lars Engstrand, Hilpi Rautelin
Summary: This study characterized Campylobacter jejuni fecal isolates from international travelers and analyzed the relationship between bacterial traits and clinical symptoms. It found that isolates from Bangladesh had higher pathogenic potential but led to milder symptoms compared to isolates from other regions with lower pathogenic potential but more severe symptoms. The study also examined the fecal microbiota composition prior to travel and found individual variation but no direct association with symptom development. Further research is needed to confirm the potential relationship between gut microbiota characteristics and the severity of Campylobacter infections.
Article
Multidisciplinary Sciences
Nuanpan Khemnu, Oralak Serichantalergs, Sirigade Ruekit, Paphavee Lertsethtakarn, Frederic Poly, Brett E. Swierczewski, John M. Crawford
Summary: Campylobacter jejuni is a leading cause of bacterial diarrhea worldwide and is linked to various complications including Guillain-Barre Syndrome, inflammatory bowel disease, reactive arthritis, and irritable bowel syndrome. C. jejuni possesses the ability to form a polysaccharide capsule on its surface, which is atypical for an intestinal pathogen. The hypervariable region of the C. jejuni genome contains the genes responsible for the biosynthesis of the phase-variable capsule, which has been classified using multiplex PCR based on Penner serotypes. However, there are still C. jejuni strains with untypeable capsules using the current multiplex PCR method. This study used next generation sequencing and whole genome analysis software to identify novel capsule biosynthesis loci in C. jejuni isolates. Unique PCR primers were designed for the identification of these new loci, and a new multiplex mix called epsilon was developed. The addition of these unique sequences complements the current C. jejuni multiplex capsule typing system and enables the identification of previously untypeable capsule loci in C. jejuni isolates.
Article
Immunology
Craig T. Parker, Kerry K. Cooper, Francesca Schiaffino, William G. Miller, Steven Huynh, Hannah K. Gray, Maribel Paredes Olortegui, Paul Garcia Bardales, Dixner Rengifo Trigoso, Pablo Penataro-Yori, Margaret N. Kosek
Summary: Campylobacter jejuni is the leading bacterial cause of gastroenteritis worldwide, and this study isolated and sequenced two C. jejuni strains from guinea pigs in the Peruvian Amazon, revealing unique genomic features that may be associated with guinea pig host specialization.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Lauren K. Hudson, William E. Andershock, Runan Yan, Mugdha Golwalkar, Nkuchia M. M'ikanatha, Irving Nachamkin, Linda S. Thomas, Christina Moore, Xiaorong Qian, Richard Steece, Katie N. Garman, John R. Dunn, Jasna Kovac, Thomas G. Denes
Summary: Campylobacteriosis is the most common bacterial foodborne illness in the United States and this study compared clinical and non-clinical Campylobacter populations from Tennessee and Pennsylvania, identified source attribution patterns through phylogenetic analyses, and identified potential outbreak clusters based on genomic similarity.
Article
Microbiology
Martine C. H. Sorensen, Yilmaz Emre Gencay, Florian Fanger, Mariana A. T. Chichkova, Maria Mazurova, Jochen Klumpp, Eva M. Nielsen, Lone Brondsted
Summary: The study revealed that Campylobacter jejuni strains from Danish broilers display varying sensitivity and resistance mechanisms to phages, with different strains being susceptible to specific phages or completely resistant. Furthermore, these strains have developed diverse resistance mechanisms such as CRISPR spacers and restriction modification systems, indicating a complex evolutionary response to phage infection.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Public, Environmental & Occupational Health
Jose A. Rodrigues, Wonhee Cha, Rebekah E. Mosci, Sanjana Mukherjee, Duane W. Newton, Paul Lephart, Hossein Salimnia, Walid Khalife, James T. Rudrik, Shannon D. Manning
Summary: In a four-year active surveillance study at four metropolitan hospitals in Michigan, an increasing trend in Campylobacter jejuni infections was observed. Analysis of 214 C. jejuni isolates revealed widespread antibiotic resistance, with differences in infection rates by hospital type and patient characteristics associated with disease severity.
FRONTIERS IN PUBLIC HEALTH
(2021)
Article
Biotechnology & Applied Microbiology
Jifeng Zeng, Yu Wang, Lixia Fan, Nuo Yang, Jiwen Pan, Yu Han, Xin Wang, Qian Li, Guiying Guo, Jiping Zheng, Weixin Zeng
Summary: This study aimed to investigate the molecular epidemiology, genotypes, and phenotypes of Streptococcus associated with bovine subclinical mastitis in Hainan, China. Results showed that environmental S. uberis plays a significant role in causing subclinical mastitis in Hainan, with distinct MLST, virulence, and antibiotic resistance characteristics.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Cell Biology
Benshak J. Audu, Strachan Norval, Lopes Bruno, Ramjee Meenakshi, Macrae Marion, Ken J. Forbes
Summary: This study in Nigeria investigated the genomic diversity and antimicrobial resistance profile of Campylobacter in humans and livestock, finding a higher prevalence of resistance in Campylobacter isolated from livestock compared to humans. The results contribute to the understanding of the epidemiology of thermophilic Campylobacter in Nigeria.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Brian Lassen, Nao Takeuchi-Storm, Clementine Henri, Tine Hald, Marianne Sandberg, Johanne Ellis-Iversen
Summary: This study compared the genetic diversity of Campylobacter isolates from organic/free-range broilers with those from conventional broilers and other animal hosts in Denmark to infer the reservoir sources of Campylobacter for free-range broilers. The results suggest that cattle may be the reservoir for Campylobacter in both conventional and free-range broilers. However, the predictive value of the data is affected by the limited sampling time and scope.
Article
Biochemistry & Molecular Biology
Stefano Ravaioli, Davide Campoccia, Werner Ruppitsch, Franz Allerberger, Alessandro Poggi, Emanuele Chisari, Lucio Montanaro, Carla Renata Arciola
Summary: This study evaluated the usefulness of automated ribotyping as a screening tool for identifying S. aureus genetic lineages. Results showed that automated ribotyping can predict spa and MLST clonal complexes, but its high cost and lower discriminatory power could be obstacles for fine genotyping analysis. However, automated ribotyping has advantages such as automation, ease of execution, stability, typeability, and reproducibility, making it a reliable method to be used alongside gold standard methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Nehal A. Saif, Jose F. Cobo-Diaz, Menattallah Elserafy, Iman El-Shiekh, Avelino Alvarez-Ordonez, Shaimaa F. Mouftah, Mohamed Elhadidy
Summary: This study characterized Campylobacter jejuni from different sources in Egypt using multiple typing techniques. The analysis revealed that cgMLST and host-segregating markers were the most discriminative methods, and identified retail chicken and cattle reservoir as important sources of C. jejuni infection in Egypt. The findings emphasize the importance of using whole genome sequencing typing methods for source tracking of C. jejuni and suggest the role of non-poultry sources in human campylobacteriosis control measures in Egypt.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Public, Environmental & Occupational Health
S. Olkkola, S. Nykasenoja, S. Raulo, A. -K. Llarena, S. Kovanen, R. Kivisto, A. -L. Myllyniemi, M. L. Hanninen
ZOONOSES AND PUBLIC HEALTH
(2016)
Article
Food Science & Technology
Sara Kovanen, Rauni Kivisto, Ann-Katrin Llarena, Ji Zhang, Ulla-Maija Karkkainen, Tamara Tuuminen, Jaakko Uksila, Marjaana Hakkinen, Mirko Rossi, Marja-Liisa Hanninen
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2016)
Article
Public, Environmental & Occupational Health
L. Pohjola, S. Nykasenoja, R. Kivisto, T. Soveri, A. Huovilainen, M. L. Hanninen, M. Fredriksson-Ahoma
ZOONOSES AND PUBLIC HEALTH
(2016)
Article
Biotechnology & Applied Microbiology
Joana Revez, Ann-Katrin Llarena, Thomas Schott, Markku Kuusi, Marjaana Hakkinen, Rauni Kivisto, Marja-Liisa Hanninen, Mirko Rossi
Article
Microbiology
Sara M. Kovanen, Rauni I. Kivisto, Mirko Rossi, Thomas Schott, Ulla-Maija Karkkainen, Tamara Tuuminen, Jaakko Uksila, Hilpi Rautelin, Marja-Liisa Hanninen
JOURNAL OF CLINICAL MICROBIOLOGY
(2014)
Article
Multidisciplinary Sciences
Satu Olkkola, Sara Kovanen, Johanna Roine, Marja-Liisa Hanninen, Anna Hielm-Bjorkman, Rauni Kivisto
Article
Biotechnology & Applied Microbiology
Sara Kovanen, Mirko Rossi, Mari Pohja-Mykra, Timo Nieminen, Mirja Raunio-Saarnisto, Mikaela Sauvala, Maria Fredriksson-Ahomaa, Marja-Liisa Hanninen, Rauni Kivisto
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Multidisciplinary Sciences
Anniina Jaakkonen, Rauni Kivisto, Maria Aarnio, Jenni Kalekivi, Marjaana Hakkinen
Article
Food Science & Technology
Beata Wysok, Joanna Wojtacka, Rauni Kivisto
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2020)
Article
Microbiology
Beata Wysok, Joanna Wojtacka, Marja-Liisa Hanninen, Rauni Kivisto
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Microbiology
Ann-Katrin Llarena, Rauni Kivisto
Article
Microbiology
Satu Olkkola, Mirko Rossi, Anniina Jaakkonen, Maria Simola, Jouni Tikkanen, Marjaana Hakkinen, Pirkko Tuominen, Otso Huitu, Jukka Niemimaa, Heikki Henttonen, Rauni Kivisto
Summary: The study found that C. jejuni was the only Campylobacter species identified in small wild mammals in Finland and in samples collected near 40 farms, with the highest prevalence in yellow-necked mice and bank voles. Through whole-genome sequencing analysis, it was suggested that C. jejuni strains from voles may represent a new previously undescribed species. The research also showed that the phylogeny of the isolates followed host species-association, rather than origin from either farm or wild captured environments.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Mikaela Sauvala, Emma Woivalin, Rauni Kivisto, Riikka Laukkanen-Ninios, Sauli Laaksonen, Roger Stephan, Maria Fredriksson-Ahomaa
Summary: The prevalence of foodborne bacteria in game bird faeces and mallard breast meat in southern Finland during the hunting season was screened using PCR. Campylobacter and Listeria were commonly detected in the faeces and Listeria on mallard meat, with L. monocytogenes of sequence types associated with human listeriosis frequently found. Good hygiene during game bird handling, storing the meat frozen, and proper heat treatment are important measures to minimize health risks for hunters and consumers.
Article
Microbiology
Rauni Kivisto, Sofia Kamarainen, Otso Huitu, Jukka Niemimaa, Heikki Henttonen
Summary: This study reveals the presence of Cryptosporidium protozoa in mouse, vole, and shrew populations around Finland, with the highest zoonotic risk associated with C. ditrichi in Apodemus flavicollis and C. andersoni in Craseomys rufocanus. The most common zoonotic species in human infections, C. parvum, was not detected in the study.
Article
Veterinary Sciences
Rauni Kivisto, Mikaela Sauvala, Maria Fredriksson-Ahomaa, Johanna Bjorkroth
Summary: C. jejuni is commonly found in reared pheasants, and different genotypes were identified in this study. The ST-45 genotype was closely related to human clinical isolates, while the dominant ST-699 genotype was distinct from other sources worldwide. Hygienic measures and improved biosecurity are necessary to control the spread of infection and reduce the zoonosis risk associated with pheasant farming.
ACTA VETERINARIA SCANDINAVICA
(2023)