Article
Food Science & Technology
Fuat Aydin, Tuba Kayman, Secil Abay, Harun Hizlisoy, Izzet Burcin Saticioglu, Emre Karakaya, Orhan Sahin
Summary: This study investigated the genetic diversity, prevalence, and mechanisms of resistance to quinolone antibiotics in 178 Campylobacter jejuni isolates from humans, cattle, dogs, and chickens in Turkey. The results showed that there were 21 clonal complexes (CCs) and 78 different sequence types (STs) among the isolates, with CC21, CC353, CC206, and CC257 being the predominant clones. Quinolone resistance was detected in 41% of the isolates, and all resistant isolates had a specific mutation in the gyrA gene. The study provided important insights into the transmission pathways of C. jejuni in Turkey, suggesting that broiler meat and dogs may be the most important sources of human campylobacteriosis in the country.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Immunology
Morgane Nennig, Ann-Katrin Llarena, Malte Herold, Joel Mossong, Christian Penny, Serge Losch, Odile Tresse, Catherine Ragimbeau
Summary: A study on Campylobacter jejuni in Luxembourg revealed an unexpected endemic pattern in the genetic profiles, and tested the hypothesis of stable lineages occurrence by implementing whole genome sequencing (WGS). Results showed that regardless of the typing scheme used, epidemic or endemic signals were detected in the lineages, supporting the clonal expansion of stable genomes in the Campylobacter population.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Virology
Yuanyue Tang, Jie Li, Yuexuan Wang, Zhaojun Song, Hangning Ying, Linghua Kong, Xin'an Jiao, Jinlin Huang
Summary: This study characterized the phage CP39 and investigated its resistance in Campylobacter jejuni. The 06875 gene was found to be responsible for CPS-phage adsorption, and rapid mutation in this gene allowed the bacteria to defend against phage CP39.
Article
Food Science & Technology
A. Conesa, G. Garofolo, A. Di Pasquale, C. Camma
Summary: Campylobacter jejuni is the main pathogen in human food-borne outbreaks worldwide, and studies have reported antimicrobial resistance (AMR) in C. jejuni strains. Monitoring of AMR in Europe focuses on Campylobacter spp. from food-producing animals, as they are the main source of campylobacteriosis in humans through contamination of food products. The emergence of resistance to multiple drugs in C. jejuni, classified as a 'high priority pathogen' by WHO, limits treatment alternatives. Understanding the contributions of different food sources to gastrointestinal disease and assessing the genetic diversity among Campylobacter species is crucial for prioritizing food safety interventions and control strategies. The work program aimed to assess AMR of C. jejuni isolated from humans, poultry, birds from wild and urban Italian habitats, and to investigate correlations between phenotypic and genotypic AMR. It also focused on acquiring knowledge and skills in web-based tools, bioinformatics analyses, and genomics data processing for identifying epidemiological clusters and studying AMR patterns in C. jejuni isolates.
Article
Agriculture, Dairy & Animal Science
Huilin Yang, Yongbin Li, Yu Zhang, Bowen Dong, Buting Duan, Lulu Guo, Tingyang Wang, Xiaoling Lv, Mingxue Zheng, Xiaozhen Cui, Rui Bai
Summary: This study collected cloacal swabs from broiler farms in Shanxi Province, China to investigate the prevalence, drug resistance, and virulence gene data of Campylobacter jejuni in broilers. The isolates showed high resistance to ampicillin and sensitivity to erythromycin, with a high rate of multidrug resistance. Multilocus sequence typing revealed genotypic diversity among isolates. The findings highlight the importance of source contamination control in broiler farms for the health of humans and animals.
Article
Biotechnology & Applied Microbiology
Mark E. Berrang, Elizabeth A. McMillan, Steven W. Knapp, Richard J. Meinersmann
Summary: This study investigated the relationship between ambient weather conditions and the isolation of Campylobacter from chicken flocks. The results showed that seasonality does not affect the rate of Campylobacter isolation from broilers in the warm temperate climate of the Southeastern U.S., but the diversity of isolates was higher in the milder spring and fall seasons.
JOURNAL OF FOOD PROTECTION
(2023)
Article
Virology
Tamar Suladze, Ekaterine Jaiani, Marina Darsavelidze, Maia Elizbarashvili, Olivier Gorge, Ia Kusradze, Tamar Kokashvili, Nino Lashkhi, George Tsertsvadze, Nino Janelidze, Svetlana Chubinidze, Marina Grdzelidze, Shota Tsanava, Eric Valade, Marina Tediashvili
Summary: Phages of highly pathogenic bacteria are gaining importance in bacterial detection and identification, subspecies typing, phage therapy, and environmental decontamination. This study identified eight new phages that showed lytic activity against Yersinia pestis, making them promising for infection control. Two of these phages also exhibited activity against other Enterobacteriaceae and their complete genome sequences were determined.
Article
Biochemistry & Molecular Biology
Stefano Ravaioli, Davide Campoccia, Werner Ruppitsch, Franz Allerberger, Alessandro Poggi, Emanuele Chisari, Lucio Montanaro, Carla Renata Arciola
Summary: This study evaluated the usefulness of automated ribotyping as a screening tool for identifying S. aureus genetic lineages. Results showed that automated ribotyping can predict spa and MLST clonal complexes, but its high cost and lower discriminatory power could be obstacles for fine genotyping analysis. However, automated ribotyping has advantages such as automation, ease of execution, stability, typeability, and reproducibility, making it a reliable method to be used alongside gold standard methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Maria Kornienko, Dmitry Bespiatykh, Maja Malakhova, Roman Gorodnichev, Nikita Kuptsov, Egor Shitikov
Summary: Phage therapy is a hopeful approach to combat multidrug-resistant bacteria. However, it requires the isolation of new phages or the search for suitable phages in existing libraries. This study proposes a simple PCR method to differentiate between two families of Staphylococcus phages and eleven genera of Klebsiella phages, allowing for rapid screening and identification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Maria Giufre, Elena Mazzolini, Marina Cerquetti, Silvio Brusaferro
Summary: This study compared ESBL-producing Escherichia coli isolates from humans and food-producing animals, finding different types of ESBLs in different sources. The results suggest the potential exchange of ESBL genes from animals to humans, emphasizing the need for strict monitoring based on a 'One Health' approach.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Ophthalmology
Yin-Hsi Chang, Yhu-Chering Huang, Hung-Chi Chen, David H. K. Ma, Lung-Kun Yeh, Kuo-Hsuan Hung, Ching-Hsi Hsiao
Summary: This study investigated the molecular and phenotypic characteristics of methicillin-resistant Staphylococcus epidermidis (MRSE) isolated from healthy conjunctiva and ocular infections. The results showed that MRSE isolates from both sources exhibited overlapping molecular types and phenotypic traits. Specific strain ST59 showed distinctive characterizations compared to other strains.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Damini Khawaskar, Anbazhagan Subbaiyan, Dhayanath Balusamy, Sophia Inbaraj, Abhishek, Obli Rajendhran Vinodh Kumar, Viswas Konasagara Nagaleekar, Dharmendra Kumar Sinha, Pallab Chaudhuri, Bhoj Raj Singh, Vinod Kumar Chaturvedi, Prasad Thomas
Summary: This study aimed to determine the genetic factors associated with bovine disease conditions caused by E. coli. Comparative genomic analysis revealed that phylogroups A and C, as well as pathotypes ExPEC and EPEC, were strongly connected with bovine disease strains. However, STEC strains, including EHEC, had minimal involvement in bovine disease. The study also identified predominant genetic lineages and virulence genes associated with disease origin strains.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Hosny El-Adawy, Helmut Hotzel, Silvia Garcia-Soto, Herbert Tomaso, Hafez M. Hafez, Stefan Schwarz, Heinrich Neubauer, Joerg Linde
Summary: This study used whole-genome sequencing to assess the epidemiology and genetic heterogeneity of C. jejuni in commercial turkey farms in Germany. The results showed high genetic diversity among the isolates, with various antimicrobial resistance genes and virulence-associated genes. The study highlights the potential of whole-genome sequencing for surveillance of C. jejuni and emphasizes the need for updates in resistance gene databases.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Microbiology
Xiaoqi Zang, Hongyue Lv, Haiyan Tang, Xinan Jiao, Jinlin Huang
Summary: This study analyzed 1609 Campylobacter jejuni isolates from various ecological origins in China. The results showed that disease associated capsular genotypes and LOS classes over-represented in human isolates were also dominant in animals, especially cattle. High-risk lineages CC-22, CC-464, and CC-21, found in human isolates with GBS worldwide, were also present in food animal isolates with disease associated capsular types, indicating a potential clonal spread across different regions and hosts.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rashit Tarakanov, Anna A. Lukianova, Peter Evseev, Roksana Pilik, Anna D. Tokmakova, Eugene E. Kulikov, Stepan Toshchakov, Alexander N. Ignatov, Fevzi S-U Dzhalilov, Konstantin A. Miroshnikov
Summary: A new lytic bacteriophage, Ayka, has been discovered to effectively control the Gram-positive bacterium Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) that causes diseases in soybean cultivation. This is the first bacteriophage specifically targeting Cff and has potential applications in agricultural bioprotection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Veterinary Sciences
Martin Peter Rydal, Michela Gambino, Claus Bottcher Jorgensen, Louise Ladefoged Poulsen, Lone Brondsted, Jens Peter Nielsen
Summary: Host genotype plays a crucial role in the susceptibility to enterotoxigenic E. coli (ETEC), with CHCF1 genotype possibly corresponding to susceptibility towards ETEC F4ac/ab infection.
BMC VETERINARY RESEARCH
(2022)
Article
Microbiology
Amira R. Vitt, Stephen J. Ahern, Michela Gambino, Martine C. H. Sorensen, Lone Brondsted
Summary: This study reports the complete genome sequences of E. coli bacteriophages isolated from various environmental samples, providing important resources for studying E. coli.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Biotechnology & Applied Microbiology
Michele Gourmelon, Amine M. Boukerb, Nesrine Nabi, Sangeeta Banerji, Katrine G. Joensen, Joelle Serghine, Alexandre Cormier, Francis Megraud, Philippe Lehours, Thomas Alter, Danielle J. Ingle, Martyn D. Kirk, Eva M. Nielsen
Summary: Members of the Campylobacter lad group from diverse sources and geographical locations were analyzed using comparative genomics. The study revealed high genetic diversity and potential human pathogenicity of these isolates, highlighting the importance of effective surveillance and disease prevention strategies.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Review
Microbiology
Jochen Klumpp, Matthew Dunne, Martin J. Loessner
Summary: Bacteriophages and their encoded RBPs have great potential as precision antimicrobials for treating bacterial infections and controlling bacterial contaminations. Their distinctive features and binding specificity make them attractive candidates for therapeutics and diagnostics. The future use of synthetic technology and artificial intelligence-driven approaches will revolutionize the application of these tools.
CURRENT OPINION IN MICROBIOLOGY
(2023)
Article
Infectious Diseases
Soren Persson, Hans Linde Nielsen, John Eugenio Coia, Jorgen Engberg, Bente Scharvik Olesen, Anne Line Engsbro, Andreas Munk Petersen, Hanne Marie Holt, Lars Lemming, Ea Sofie Marmolin, Turid Snekloth Sondergaard, Leif Percival Andersen, Mie Birgitte Frid Jensen, Camilla Wiuff, Gitte Sorensen, Sofie Holtsmark Nielsen, Eva Moller Nielsen
Summary: This study established a new sentinel surveillance scheme to monitor C. difficile infections in Denmark. The results showed geographical differences and temporal changes in the distribution of different types of C. difficile infections.
Article
Biotechnology & Applied Microbiology
Marta Volpi, Michela Gambino, Kirsten Kirkeby, Anne Elsser-Gravesen, Lone Brondsted
Summary: This study conducted a full-scale industrial trial to assess the efficacy of a commercial phage product in reducing naturally occurring Salmonella on pork carcasses. The results showed that phage application led to a reduction of approximately 79% in Salmonella-positive carcasses. This demonstrates that the implementation of phage application in industrial settings can serve as an additional strategy for controlling foodborne pathogens.
Article
Biotechnology & Applied Microbiology
Yilmaz Emre Gencay, Dziuginta Jasinskyte, Camille Robert, Szabolcs Semsey, Virginia Martinez, Anders Ostergaard Petersen, Katja Brunner, Ana de Santiago Torio, Alex Salazar, Iszabela Cristiana Turcu, Melissa Kviesgaard Eriksen, Lev Koval, Adam Takos, Ricardo Pascal, Thea Staffeldt Schou, Lone Bayer, Tina Bryde, Katja Chandelle Johansen, Emilie Glad Bak, Frenk Smrekar, Timothy B. Doyle, Michael J. Satlin, Aurelie Gram, Joana Carvalho, Lene Jessen, Bjorn Hallstrom, Jonas Hink, Birgitte Damholt, Alice Troy, Mette Grove, Jasper Clube, Christian Grondahl, Jakob Krause Haaber, Eric van der Helm, Milan Zdravkovic, Morten Otto Alexander Sommer
Summary: By screening and modifying a library of 162 wild-type phages, researchers have developed a phage therapy that can specifically target Escherichia coli. The engineered phages reduce the emergence of phage-tolerant bacteria and out-compete their wild-type counterparts. SNIPR001, a combination of four complementary bacteriophages, shows promising results in reducing E. coli load in animal models.
NATURE BIOTECHNOLOGY
(2023)
Article
Microbiology
Cedric Woudstra, Lone Brondsted
Summary: In light of the global antimicrobial resistance crisis, there is an urgent need for new antibiotic substitutes. Tailocins, bacteriocin particles similar to phage tails produced by bacteria for environmental competition, are potential alternatives to antibiotics. However, the availability of characterized Tailocins is limited. This study explored the production of Tailocins from phage particles using osmotic shock or chemical treatment, and found that different phages have different responses to these treatments.
PHAGE-THERAPY APPLICATIONS AND RESEARCH
(2023)
Article
Microbiology
Amira R. Vitt, Martine C. Holst Sorensen, Valeria Bortolaia, Lone Brondsted
Summary: This study established a representative collection of ESBL/AmpC-producing Escherichia coli from livestock and meat and tested the antimicrobial potential of bacteriophages. The results showed that Warwickvirus phages exhibited the broadest host range and were able to kill up to 26 strains. It was concluded that specific phages for ESBL/AmpC E. coli should be isolated as some strains were resistant to all tested phages. The strain collection described in this study is a valuable resource fulfilling this need.
PHAGE-THERAPY APPLICATIONS AND RESEARCH
(2023)
Article
Virology
Pilar Garcia, Rafael Tabla, Hany Anany, Roberto Bastias, Lone Brondsted, Susana Casado, Pablo Cifuentes, John Deaton, Thomas G. Denes, Mohammad Aminul Islam, Rob Lavigne, Andrea I. Moreno-Switt, Natsuko Nakayama, Cristina Munoz Madero, Alexander Sulakvelidze, Antonet M. Svircev, Jeroen Wagemans, Elena G. Biosca, Dacil Rivera
Summary: This meeting discussed the suitability of using bacteriophages as alternative antimicrobials in the agrifood sector. It brought together researchers, industry delegates, and policymakers to debate the advantages, potential shortcomings, and regulatory aspects of phage application strategies.
Article
Microbiology
Carla Rodrigues, Kathrin Hauser, Niamh Cahill, Malgorzata Ligowska-Marzeta, Gabriella Centorotola, Alessandra Cornacchia, Raquel Garcia Fierro, Marisa Haenni, Eva Moller Nielsen, Pascal Piveteau, Elodie Barbier, Dearbhaile Morris, Francesco Pomilio, Sylvain Brisse
Summary: The study provides a novel isolation strategy for Klebsiella pneumoniae species complex (KpSC) from food matrices, and reveals high prevalence of KpSC in chicken meat and salad, indicating that food could be a potential source of KpSC colonization in humans.
MICROBIOLOGY SPECTRUM
(2022)