Article
Veterinary Sciences
Longfei Zhang, Hongjuan Wang, Yilin Bai, Lei Wang, Yueyu Bai, Jianhe Hu
Summary: The PK/PD integration model based on mutant selection window theory is an important method to optimize the dosage regimen for the prevention of multidrug-resistant bacteria. In this study, an in vitro dynamic infection model was used to investigate the prevention of drug-resistant mutations of danofloxacin against Actinobacillus pleuropneumoniae (AP), and the relationship between PK/PD parameters and antibacterial activity was analyzed.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Lukas Schuwerk, Doris Hoeltig, Karl-Heinz Waldmann, Peter Valentin-Weigand, Judith Rohde
Summary: Serotyping and molecular methods play a crucial role in characterizing the behavior and genetic features of A. pleuropneumoniae isolates in outbreaks of porcine pleuropneumonia in Germany, highlighting the importance of specific serovars in different clinical cases. Furthermore, some isolates deviate from the typical gene profiles of reference strains, indicating a need for further investigation into genetic variability within this pathogen.
VETERINARY RESEARCH
(2021)
Article
Immunology
Ulrik Fahnoe, Andreas Ronit, Ronan M. G. Berg, Sofie E. Jorgensen, Trine H. Mogensen, Alexander P. Underwood, Troels K. H. Scheel, Jens Bukh, Ronni R. Plovsing
Summary: The study found that in CARDS patients, treatment with dexamethasone did not regulate inflammatory pathways, but rather regulated other local immune responses. Additionally, a distinct dexamethasone-dependent transcriptomic signature was detected in the lungs of mechanically ventilated COVID-19 patients.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Cardiac & Cardiovascular Systems
Song Mi, Na Cui, Xiaokai Feng, Chunguo Jiang, Kewu Huang, Liming Zhang
Summary: This study evaluated the lymphocyte profile in lymph node puncture fluid, bronchoalveolar lavage fluid, and peripheral blood in patients with stage 2 sarcoidosis. The results showed that the CD4/CD8 ratio in bronchoalveolar lavage fluid was positively correlated with respiratory symptoms, indicating that the difference in lymphocyte profile between bronchoalveolar lavage fluid and lymph node puncture fluid may help understand the pathophysiological process.
RESPIRATORY MEDICINE
(2022)
Article
Oncology
Iwona Kwiecien, Elzbieta Rutkowska, Agata Raniszewska, Agnieszka Rzeszotarska, Malgorzata Polubiec-Kownacka, Joanna Domagala-Kulawik, Jolanta Korsak, Piotr Rzepecki
Summary: This study investigated the antigenic and cytokine profile of macrophages in bronchoalveolar lavage fluid from lung affected by cancer and healthy lung. The findings revealed higher proportion of macrophages in cancer-affected lung, as well as the presence of different antigens and cytokines in macrophages, indicating their diversity and unique properties.
Article
Respiratory System
Ziliang Hou, Dan Peng, Jingjing Yang, Shuai Zhang, Jinxiang Wang
Summary: The study developed a predictive model consisting of seven genes for risk stratification and individualized survival prediction in patients with idiopathic pulmonary fibrosis (IPF). The model demonstrated excellent discrimination and calibration in the derivation and validation cohorts.
JOURNAL OF THORACIC DISEASE
(2022)
Article
Oncology
Daxiong Zeng, Cangguo Wang, Chuanyong Mu, Meiqin Su, Jingyu Mao, Jianan Huang, Jiayue Xu, Lin Shao, Bing Li, Haiyan Li, Bingsi Li, Jun Zhao, Junhong Jiang
Summary: The study demonstrated the potential of BALF cfDNA in differentiating malignant lung tumors from benign pulmonary nodules. Analyzing mutations and methylation in BALF showed better sensitivity and accuracy compared to traditional methods, especially in distinguishing small malignant tumors from benign nodules.
ANNALS OF TRANSLATIONAL MEDICINE
(2021)
Article
Medicine, General & Internal
O. V. Voronkova, J. G. Birulina, V. V. Ivanov, E. E. Buyko, I. E. Esimova, A. Grigorieva, I. A. Osikhov, N. A. Chernyshov, E. A. Motlokhova
Summary: This study aimed to identify the features of bronchoalveolar lavage fluid composition and cytokine profile in rats with diet-induced metabolic syndrome. The results showed that the changes in the parameters of bronchoalveolar lavage fluid were inflammatory in nature and were formed during a systemic inflammatory response accompanying metabolic disorders.
BYULLETEN SIBIRSKOY MEDITSINY
(2022)
Article
Virology
Andrew M. Kropinski, Dann Turner, John H. E. Nash, Hans-Wolfgang Ackermann, Erika J. Lingohr, Richard A. Warren, Kenneth C. Ehrlich, Melanie Ehrlich
Article
Biochemical Research Methods
Genevieve Labbe, Marisa A. Rankin, James Robertson, Jonathan Moffat, Elissa Giang, Lok Kan Lee, Kim Ziebell, Joanne MacKinnon, Chad R. Laing, E. Jane Parmley, Agnes Agunos, Danielle Daignault, Sadjia Bekal, Linda Chui, Kimberley A. MacDonald, Linda Hoang, Durda Slavic, Danielle Ramsay, Frank Pollari, John H. E. Nash, Roger P. Johnson
JOURNAL OF MICROBIOLOGICAL METHODS
(2019)
Article
Multidisciplinary Sciences
James Robertson, Janet Lin, Amie Wren-Hedgus, Gitanjali Arya, Catherine Carrillo, John H. E. Nash
Article
Multidisciplinary Sciences
Clifford G. Clark, Chrystal Landgraff, James Robertson, Frank Pollari, Stephen Parker, Celine Nadon, Victor P. J. Gannon, Roger Johnson, John Nash
Article
Genetics & Heredity
James Robertson, Kyrylo Bessonov, Justin Schonfeld, John H. E. Nash
MICROBIAL GENOMICS
(2020)
Article
Biotechnology & Applied Microbiology
Tam T. Tran, Andrew Scott, Yuan-Ching Tien, Roger Murray, Patrick Boerlin, David L. Pearl, Kira Liu, James Robertson, John H. E. Nash, Edward Topp
Summary: The study found that on-farm anaerobic digestion can reduce the abundance of enteric bacteria, antibiotic resistance-associated gene targets, and the horizontal transfer potential of ESBL genes. Most gene targets were more abundant in raw manure, but some resistance gene targets were more abundant in digested samples.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Clifford G. Clark, Ashley K. Kearney, Lorelee Tschetter, James Robertson, Frank Pollari, Stephen Parker, Gitanjali Arya, Kim Ziebell, Roger Johnson, John Nash, Celine Nadon
Summary: The study analyzed genetic variations, distribution, and associations of Monophasic Salmonella 4,[5]:12:i:- and S. Typhimurium through whole genome sequencing, identifying different genetic variations and their correlations. Common characteristics were found in both clinical case clusters and non-human sources.
Article
Environmental Sciences
Ainslie J. Butler, Katarina Pintar, Janis L. Thomas, Manon Fleury, Stefanie Kadykalo, Kim Ziebell, John Nash, David Lapen
Summary: Recreational water sites along the main river had higher pathogen presence compared to beach sites, likely due to different pollution sources and hydrological disposition. Birds were identified as an important source of pathogens at beach sites, suggesting that wild bird management could reduce public health risks. Generic Escherichia coli concentrations did not significantly differ between beaches and the river, but were associated with the presence of other pathogens, indicating their potential as indicators of contamination and human health risk.
JOURNAL OF WATER AND HEALTH
(2021)
Article
Genetics & Heredity
Genevieve Labbe, Peter Kruczkiewicz, James Robertson, Philip Mabon, Justin Schonfeld, Daniel Kein, Marisa A. Rankin, Matthew Gopez, Darian Hole, David Son, Natalie Knox, Chad R. Laing, Kyrylo Bessonov, Eduardo N. Taboada, Catherine Yoshida, Kim Ziebell, Anil Nichani, Roger P. Johnson, Gary Van Domselaar, John H. E. Nash
Summary: Hierarchical genotyping approaches, such as BioHansel, provide rapid and high-resolution classification of bacterial pathogens. The tool is organism-agnostic and uses SNP-based genotyping schemes to distinguish predefined lineages, showing full concordance with traditional methods while being significantly faster. Additionally, the quality assurance module of BioHansel is effective in detecting contamination even in populations with low genetic diversity, making it ideal for use by public health agencies.
MICROBIAL GENOMICS
(2021)
Article
Infectious Diseases
Julie L. Shaw, Vincent Deslandes, Jennifer Smith, Marc Desjardins
Summary: The study evaluated the performance of the Abbott PanbioTM COVID-19 rapid antigen assay in asymptomatic individuals, showing its ability to rapidly identify infected individuals but not suitable for ruling out SARS-CoV-2 infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2021)
Article
Multidisciplinary Sciences
Vincent Deslandes, Darya Rafipour, Ivan Gorn, Elham Sabri, Nadia Sant, Marc Desjardins
Summary: Delayed entry of blood culture bottles is a common issue in consolidated laboratories. This study found that prolonged preincubation time can increase the total detection time while reducing the incubation time. Therefore, optimizing courier routes is recommended to improve the efficiency of centralized microbiological services.
SCIENTIFIC REPORTS
(2022)
Article
Genetics & Heredity
Kyrylo Bessonov, Chad Laing, James Robertson, Irene Yong, Kim Ziebell, Victor P. J. Gannon, Anil Nichani, Gitanjali Arya, John H. E. Nash, Sara Christianson
Summary: This study developed a tool called ECTyper for Escherichia and Shigella species identification and in silico serotype prediction, and compared its performance with other tools. Results showed that ECTyper exhibited the highest concordance on newly isolated strains, while on a larger genomic dataset, it performed similarly to SerotypeFinder.
MICROBIAL GENOMICS
(2021)
Article
Pharmacology & Pharmacy
Ryan Marko, Julia Hajjar, Vanessa Nzeribe, Michelle Pittman, Vincent Deslandes, Nadia Sant, Juthaporn Cowan, Kwadwo Kyermentang, Tim Ramsay, Sheryl Zelenitsky, Salmaan Kanji
Summary: This study aimed to investigate the discordance rate between trough levels and AUC(24)/MIC values, and compare the treatment failure and nephrotoxicity outcomes between patients achieving and not achieving their pharmacodynamic targets. Results showed that a portion of patients had discordance between trough levels and AUC(24)/MIC values, while 57% of patients received an initial vancomycin dose less than 15 mg/kg. The treatment failure rate was 34%, with a nephrotoxicity rate of 18%.
CANADIAN JOURNAL OF HOSPITAL PHARMACY
(2021)
Article
Infectious Diseases
Vincent Deslandes, Eric Clark, Venkatesh Thiruganasambandamoorthy, Marc Desjardins
Summary: The study found that the ID Now COVID-19 assay is a rapid and accurate tool for detecting infection in symptomatic patients, with high agreement and sensitivity compared to laboratory-based molecular methods.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2022)
Article
Genetics & Heredity
James Robertson, Justin Schonfeld, Kyrylo Bessonov, Patrick Bastedo, John H. E. Nash
Summary: In this study, the MOB-suite toolset was used to reconstruct and classify plasmids in a large-scale population of Salmonella. The transfer of plasmids and antimicrobial resistance genes between different MOB-clusters and serotypes were characterized. The majority of Salmonella plasmids were not involved in the dissemination of antimicrobial resistance genes, and the transfer of genes was more significant between different MOB-clusters than between different serotypes. This plasmid characterization approach can be applied to other organisms to identify high-risk genes and plasmids for horizontal transfer.
MICROBIAL GENOMICS
(2023)