Article
Biochemistry & Molecular Biology
Hanbeen Kim, Jakyeom Seo
Summary: Two potential endolysins were identified, with one showing better efficacy against MRSA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Zhimin Bai, Min Chen, Qiaofa Lin, Ying Ye, Hongmei Fan, Kaizhen Wen, Jianxing Zeng, Donghong Huang, Wenfei Mo, Ying Lei, Zhijun Liao
Summary: By extracting feature vectors from protein sequences and using classification tools, MRSA and MSSA were successfully distinguished. MRSA showed high resistance to penicillin and high pathogenic risk. Cross-infection between different types of MRSA was found in Quanzhou, and MRSA in traditional hospitals exhibited increasingly blurred molecular characteristics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Microbiology
Oren Gordon, Dustin A. Dikeman, Roger Ortines, Yu Wang, Christine Youn, Mohammed Mumtaz, Nicholas Orlando, Jeffrey Zhang, Aman Patel, Ethan Gough, Amit Kaushik, Eric L. Nuermberger, Anna M. Upton, Nader Fotouhi, Lloyd S. Miller, Nathan K. Archer
Summary: Staphylococcus aureus is a major cause of bloodstream, skin, and bone infections in humans. The emergence of drug-resistant strains increases the difficulty in treating these infections. This study compared the efficacy of a novel oxazolidinone antibiotic, TBI-223, with linezolid in mouse models of MRSA infection. TBI-223 showed comparable efficacy to linezolid in reducing bacterial burden and disease severity in all three models.
MICROBIOLOGY SPECTRUM
(2022)
Article
Food Science & Technology
Martina Florianova, Kristyna Korena, Helena Juricova
Summary: This study evaluated the prevalence of Staphylococcus aureus in dry-fermented salami and found multidrug-resistant and virulent strains. The source of contamination was identified as raw pork and human carriers. Therefore, maintaining high standards of hygiene and manufacturing practices in food processing is crucial.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Gao-Feng Zha, Habbanakuppe D. Preetham, Shobith Rangappa, Kothanahally S. Sharath Kumar, Yarabahally R. Girish, Kadalipura P. Rakesh, Milad Ashrafizadeh, Ali Zarrabi, Kanchugarakoppal S. Rangappa
Summary: The development of small molecule inhibitors as antibacterial agents against MRSA is a growing field in medicinal chemistry. Benzimidazoles, a class of heterocyclic compounds, have shown promising capability in fighting against MRSA due to their high biocompatibility, synergistic behavior with antibiotics, and tunable physicochemical properties. These compounds act as DNA binding agents, enzyme inhibitors, and anti-biofilm agents, showing a synergistic effect with available antibiotics to achieve antibacterial activity against MRSA. This review focuses on recent developments of benzimidazole-hybrid molecules as anti-MRSA agents, discussing their possible mechanism of action and structure-activity relationship.
BIOORGANIC CHEMISTRY
(2021)
Article
Infectious Diseases
Jian Peng, Biswajit Mishra, Rajamohammed Khader, LewisOscar Felix, Eleftherios Mylonakis
Summary: In this study, a smaller antimicrobial peptide C18 derived from cecropin 4 showed high antibacterial activity against various bacteria and yeasts, with a remarkable effect on drug-resistant Staphylococcus aureus. C18 also exhibited the capability to eliminate persister cells, reduce biofilm adherence, interact with DNA, and down-regulate virulence factor genes. Moreover, C18 showed promising results in a Galleria mellonella model, highlighting its potential as a therapeutic candidate for severe MRSA infections.
Article
Food Science & Technology
Shen Yang, Mingyao Wang, Jialong Gao, Jiaxin Liu, Ritian Jin, Rong Lin, Wuyin Weng, Jude Juventus Aweya
Summary: The activity of antimicrobial peptides (AMPs) can be enhanced by low concentration of sodium chloride (NaCl), which can increase the antibacterial effect against Staphylococcus aureus. The enhancement is achieved through the destruction of bacterial structure, transformation of peptide structure, and increased binding and permeability to bacterial membranes.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Infectious Diseases
Stefan Monecke, Amira K. Bedewy, Elke Mueller, Sascha D. Braun, Celia Diezel, Amel Elsheredy, Ola Kader, Martin Reinicke, Abeer Ghazal, Shahinda Rezk, Ralf Ehricht
Summary: The study characterised MRSA isolates from a tertiary care centre in Alexandria, Egypt. The isolates belonged to 11 different clonal complexes and 14 strains, with CC15-MRSA-[V+fus] and CC1-MRSA-[V+fus+tir+ccrA/B-1] (PVL+) being the most common strains. Most isolates carried variant SCCmec elements and the fusidic acid resistance gene fusC. PVL genes were also prevalent. The study revealed an increase in fusC and PVL carriage and a decrease in the prevalence of the CC239 strain when compared to data from a previous study.
Article
Microbiology
Mohamad Hamad, Farah Al-Marzooq, Vunnam Srinivasulu, Hany A. Omar, Ashna Sulaiman, Dana M. Zaher, Gorka Orive, Taleb H. Al-Tel
Summary: We report the discovery of a new class of potent antimicrobial compounds effective against Gram-positive bacteria, including MRSA. These compounds exhibit low minimum inhibitory concentrations and rapid killing ability, without inducing bacterial resistance. Furthermore, they show minimal toxicity to human red blood cells. The study suggests that these small molecules hold promise as new antibacterial agents against MRSA infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Infectious Diseases
Martyna Kasela, Mateusz Ossowski, Ewelina Dzikon, Katarzyna Ignatiuk, Lukasz Wlazlo, Anna Malm
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant cause of infectious diseases in humans and animals, resulting in economic losses in human healthcare and the farming sector. The changing epidemiology of MRSA globally affects animal welfare and public health concerns. Livestock have high rates of MRSA colonization, raising questions about reservoirs and transmission, while companion animals vary in MRSA colonization and infection rates, impacting human health. This article highlights the circulation of animal-associated MRSA (AA-MRSA) in the environment and factors influencing its transmission, as well as successful strategies to limit its spread among animals and humans.
Article
Microbiology
Isabel Guthridge, Simon Smith, Matthew Law, Enzo Binotto, Josh Hanson
Summary: Lincosamides, particularly when used as a predominant therapy, show potential efficacy in the treatment of MRSA bacteremia, especially in young, clinically stable patients with few comorbidities. Patients receiving lincosamide-predominant therapy had lower in-hospital mortality and a lower incidence of renal complications compared to those receiving an alternative regimen. Prospective studies are needed to further define the optimal role of lincosamides in the treatment of MRSA bacteremia.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Environmental Sciences
Anne Mette Madsen, Fei Zhang, Yonghui Zeng, Margit W. Frederiksen
Summary: This study investigated the potential human exposure to airborne microorganisms, including MRSA, in a pigeon exhibition in Denmark. The results showed high concentrations of fungi, bacteria, and endotoxin in the air, with concentrations up to 200 times higher than outdoor references. The bacteria found in the exhibition were mainly species found in pigeon coops, indicating that pigeons are the sources of exposure. The presence of airborne MRSA highlights the importance of considering this environment as a potential place of exchange of resistant bacteria between animals and humans.
ENVIRONMENTAL RESEARCH
(2023)
Article
Immunology
Philip Nikolic, Poonam Mudgil, David G. Harman, John Whitehall
Summary: This study aimed to identify and compare the general differences in protein profiles among clinical strains of S. aureus sensitive and resistant to methicillin. The proteomic analysis revealed differences in protein abundances related to virulence, metabolism, and protein synthesis between MSSA and MRSA strains.
MICROBIAL PATHOGENESIS
(2023)
Review
Microbiology
Bohdan L. Bilyk, Viralkumar V. Panchal, Mariana Tinajero-Trejo, Jamie K. Hobbs, Simon J. Foster
Summary: The development of resistance to beta-lactam antibiotics has made Staphylococcus aureus, especially MRSA, a global clinical burden. The acquisition of mecA, which encodes PBP2A, allows MRSA to proliferate in the presence of beta-lactams. Further genetic adjustments are required to develop high-level resistance.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2022)
Article
Microbiology
Jiade Zhu, Banghui Liu, Xueqin Shu, Baolin Sun
Summary: Through comparative genome analysis, this study identified 10 mutations in 9 genes and verified the role of a novel mutation, WalK (I237T), in conferring VISA characteristics in MSSA. The mutation resulted in thickened cell walls, reduced autolysis, attenuated virulence, and altered gene expression associated with cell wall metabolism and virulence control. Further assays showed that WalK can directly bind to specific promoter regions and regulate gene expression, providing insight into the molecular mechanisms of developmental VISA resistance in MSSA with point mutations.
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Infectious Diseases
Elena Martinez, Nadine Holmes, Peter Jelfs, Vitali Sintchenko
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2015)
Article
Microbiology
Qinning Wang, Nadine Holmes, Elena Martinez, Peter Howard, Grant Hill-Cawthorne, Vitali Sintchenko
JOURNAL OF CLINICAL MICROBIOLOGY
(2015)
Article
Microbiology
Nadine McCallum, Timothy J. Gray, Qinning Wang, Jimmy Ng, Leanne Hicks, Trang Nguyen, Marion Yuen, Grant A. Hill-Cawthorne, Vitali Sintchenko
JOURNAL OF CLINICAL MICROBIOLOGY
(2015)
Article
Pathology
J. C. Kwong, N. McCallum, V. Sintchenko, B. P. Howden
Article
Microbiology
Anastasia Phillips, Cristina Sotomayor, Qinning Wang, Nadine Holmes, Catriona Furlong, Kate Ward, Peter Howard, Sophie Octavia, Ruiting Lan, Vitali Sintchenko
Article
Multidisciplinary Sciences
Ulziijargal Gurjav, Alexander C. Outhred, Peter Jelfs, Nadine McCallum, Qinning Wang, Grant A. Hill-Cawthorne, Ben J. Marais, Vitali Sintchenko
Article
Pathology
Winkie Fong, Verlaine Timms, Nadine Holmes, Vitali Sintchenko
Article
Infectious Diseases
Ulziijargal Gurjav, Peter Jelfs, Nadine McCallum, Ben J. Marais, Vitali Sintchenko
BMC INFECTIOUS DISEASES
(2014)
Article
Multidisciplinary Sciences
Alexander C. Outhred, Nadine Holmes, Rosemarie Sadsad, Elena Martinez, Peter Jelfs, Grant A. Hill-Cawthorne, Gwendolyn L. Gilbert, Ben J. Marais, Vitali Sintchenko
Article
Biochemical Research Methods
Alexander Payne, Nadine Holmes, Vardhman Rakyan, Matthew Loose
Article
Biochemical Research Methods
Rachael E. Workman, Alison D. Tang, Paul S. Tang, Miten Jain, John R. Tyson, Roham Razaghi, Philip C. Zuzarte, Timothy Gilpatrick, Alexander Payne, Joshua Quick, Norah Sadowski, Nadine Holmes, Jaqueline Goes de Jesus, Karen L. Jones, Cameron M. Soulette, Terrance P. Snutch, Nicholas Loman, Benedict Paten, Matthew Loose, Jared T. Simpson, Hugh E. Olsen, Angela N. Brooks, Mark Akeson, Winston Timp
Correction
Biochemical Research Methods
Rachael E. Workman, Alison D. Tang, Paul S. Tang, Miten Jain, John R. Tyson, Roham Razaghi, Philip C. Zuzarte, Timothy Gilpatrick, Alexander Payne, Joshua Quick, Norah Sadowski, Nadine Holmes, Jaqueline Goes de Jesus, Karen L. Jones, Cameron M. Soulette, Terrance P. Snutch, Nicholas Loman, Benedict Paten, Matthew Loose, Jared T. Simpson, Hugh E. Olsen, Angela N. Brooks, Mark Akeson, Winston Timp
Article
Infectious Diseases
Erik Alm, Eeva K. Broberg, Thomas Connor, Emma B. Hodcroft, Andrey B. Komissarov, Sebastian Maurer-Stroh, Angeliki Melidou, Richard A. Neher, Aine O'Toole, Dmitriy Pereyaslov
Article
Multidisciplinary Sciences
Karen H. Miga, Sergey Koren, Arang Rhie, Mitchell R. Vollger, Ariel Gershman, Andrey Bzikadze, Shelise Brooks, Edmund Howe, David Porubsky, Glennis A. Logsdon, Valerie A. Schneider, Tamara Potapova, Jonathan Wood, William Chow, Joel Armstrong, Jeanne Fredrickson, Evgenia Pak, Kristof Tigyi, Milinn Kremitzki, Christopher Markovic, Valerie Maduro, Amalia Dutra, Gerard G. Bouffard, Alexander M. Chang, Nancy F. Hansen, Amy B. Wilfert, Francoise Thibaud-Nissen, Anthony D. Schmitt, Jon-Matthew Belton, Siddarth Selvaraj, Megan Y. Dennis, Daniela C. Soto, Ruta Sahasrabudhe, Gulhan Kaya, Josh Quick, Nicholas J. Loman, Nadine Holmes, Matthew Loose, Urvashi Surti, Rosa Ana Risques, Tina A. Graves Lindsay, Robert Fulton, Ira Hall, Benedict Paten, Kerstin Howe, Winston Timp, Alice Young, James C. Mullikin, Pavel A. Pevzner, Jennifer L. Gerton, Beth A. Sullivan, Evan E. Eichler, Adam M. Phillippy
Article
Biotechnology & Applied Microbiology
Joseph G. Chappell, Theocharis Tsoleridis, Gemma Clark, Louise Berry, Nadine Holmes, Christopher Moore, Matthew Carlile, Fei Sang, Bisrat J. Debebe, Victoria Wright, William L. Irving, Brian J. Thomson, Timothy C. J. Boswell, Iona Willingham, Amelia Joseph, Wendy Smith, Manjinder Khakh, Vicki M. Fleming, Michelle M. Lister, Hannah C. Howson-Wells, Edward C. Holmes, Matthew W. Loose, Jonathan K. Ball, C. Patrick McClure
Summary: The research focused on testing patients with COVID-19 compatible symptoms admitted to a large teaching hospital in the UK, and found evidence of early widespread community circulation of SARS-CoV-2. Genome sequencing showed distinct lineages of the virus in early cases, with some cases acquiring the virus in the UK rather than from high-risk areas like Daegu. Analysis revealed multiple viral introductions in the Nottinghamshire area mainly in late February and March, emphasizing the importance of timely and extensive community testing to prevent future transmission.
JOURNAL OF GENERAL VIROLOGY
(2021)