Article
Hematology
Kai Kammers, Margaret A. Taub, Rasika A. Mathias, Lisa R. Yanek, Kanika Kanchan, Vidya Venkatraman, Niveda Sundararaman, Joshua Martin, Senquan Liu, Dixie Hoyle, Koen Raedschelders, Ronald Holewinski, Sarah Parker, Victoria Dardov, Nauder Faraday, Diane M. Becker, Linzhao Cheng, Zack Z. Wang, Jeffrey T. Leek, Jennifer E. Van Eyk, Lewis C. Becker
Summary: Deriving megakaryocytes (MKs) from pluripotent stem cells (iPSC) allows for the comprehensive study of gene and protein expression important in MK and platelet function. Factors specific to individuals influence the differentiation of MKs from iPSCs, with differential gene and protein expression seen based on subject's sex and race. Numerous genes and proteins highly expressed in MKs provide potential candidates for future research on hematopoiesis, platelet formation, and/or platelet function.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Biochemistry & Molecular Biology
Julian O. Kimura, Marcela Bolanos, Lorenzo Ricci, Mansi Srivastava
Summary: This study reveals the cellular mechanism and molecular trajectory for the formation of adult pluripotent stem cells (aPSCs) during embryonic development. By utilizing lineage tracing and single-cell transcriptome profiling, the researchers identified a molecular trajectory for neoblast formation and showed that aPSCs derive from one lineage instead of multiple tissue-specific lineages.
Article
Cell Biology
Min Yang, Tiago Rito, Jakob Metzger, Jeffrey Naftaly, Rohan Soman, Jianjun Hu, David F. Albertini, David H. Barad, Ali H. Brivanlou, Norbert Gleicher
Summary: The research provides evidence that embryos containing aneuploid cells are still suitable for in vitro fertilization transfer. It was found that healthy births can result from aneuploid embryos, challenging the belief that aneuploid embryos should be withheld from transfer. Additionally, the presence of euploid cells can help rescue embryonic tissues in mosaic embryos.
NATURE CELL BIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Lu Zhang, Xiao-Jing-Nan Ma, Yuan-Yuan Fei, Heng-Tong Han, Jun Xu, Lu Cheng, Xun Li
Summary: The liver has the ability to repair itself, but pathological changes can impair its regenerative capacity and lead to liver failure. Mesenchymal stem cells (MSCs) have shown promise in liver regeneration, and induced pluripotent stem cells have made progress in organoid establishment and drug screening.
PHARMACOLOGY & THERAPEUTICS
(2022)
Review
Biochemistry & Molecular Biology
Michael Dannemann, Irene Gallego Romero
Summary: The emergence of induced pluripotent stem cells (iPSCs) has transformed the study of human evolution by providing a viable and powerful model system. With the ability to be established easily, maintained indefinitely, and differentiated into various tissue types, iPSCs are rapidly becoming a crucial tool in addressing previously challenging questions in human evolution.
Article
Cell Biology
Ying Zhang, Jun Wei, Jiani Cao, Kehua Zhang, Yaojin Peng, Hongkui Deng, Jiuhong Kang, Guangjin Pan, Yong Zhang, Boqiang Fu, Shijun Hu, Jie Na, Yan Liu, Lei Wang, Lingmin Liang, Huanxin Zhu, Yu Zhang, Zi-Bing Jin, Jie Hao, Aijin Ma, Tongbiao Zhao, Junying Yu
Summary: "Requirements for Human-Induced Pluripotent Stem Cells" is the first set of guidelines in China on human-induced pluripotent stem cells. It provides detailed technical requirements and aims to promote international standardization.
CELL PROLIFERATION
(2022)
Article
Cell & Tissue Engineering
Wei-Kai Huang, Samuel Zheng Hao Wong, Sarshan R. Pather, Phuong T. T. Nguyen, Feng Zhang, Daniel Y. Zhang, Zhijian Zhang, Lu Lu, Wanqi Fang, Luyun Chen, Analiese Fernandes, Yijing Su, Hongjun Song, Guo-Li Ming
Summary: Human brain organoids derived from induced pluripotent stem cells (iPSCs) provide a valuable model for studying features of human brain development and diseases, such as the arcuate nucleus of the hypothalamus. By generating arcuate organoids (ARCOs) and analyzing their molecular heterogeneity, a human arcuate nucleus molecular signature was revealed. Furthermore, ARCOs derived from Prader-Willi syndrome (PWS) patient iPSCs showed aberrant differentiation and transcriptomic dysregulation, providing insights into disease mechanisms during early human arcuate development.
Article
Biochemistry & Molecular Biology
Alberto Martin, Carolina Epifano, Borja Vilaplana-Marti, Ivan Hernandez, Rocio I. R. Macias, Angel Martinez-Ramirez, Ana Cerezo, Pablo Cabezas-Sainz, Maria Garranzo-Asensio, Sandra Amarilla-Quintana, Deborah Gomez-Dominguez, Eduardo Caleiras, Jordi Camps, Gonzalo Gomez-Lopez, Marta Gomez de Cedron, Ana Ramirez de Molina, Rodrigo Barderas, Laura Sanchez, Susana Velasco-Miguel, Ignacio Perez de Castro
Summary: Despite being frequently observed in cancer cells, chromosomal instability (CIN) and aneuploidy have adverse effects on cellular homeostasis. Epitranscriptomic marks catalyzed by RNA-modifying enzymes change under stress, but their association with aneuploidy is still unknown. TRMT61B, a mitochondrial RNA methyltransferase enzyme, is found to be associated with high levels of aneuploidy in cancer cells. Depletion of TRMT61B induces senescence in cells with low aneuploidy, and apoptosis in cells with high aneuploidy. These findings suggest TRMT61B as a potential biomarker for targeting highly aneuploid tumors.
CELL DEATH AND DIFFERENTIATION
(2023)
Review
Neurosciences
Alejandro De Los Angeles, Michael B. Fernando, Nicola A. L. Hall, Kristen J. Brennand, Paul J. Harrison, Brady J. Maher, Daniel R. Weinberger, Elizabeth M. Tunbridge
Summary: A key challenge in psychiatry research is to develop high-fidelity model systems for exploring pathophysiological mechanisms of illness, where iPSCs offer potential but selecting relevant phenotypes and model systems remains a challenge. The importance of considering research hypotheses to guide the choice of the most appropriate model system is emphasized.
BIOLOGICAL PSYCHIATRY
(2021)
Article
Biochemical Research Methods
Julia Vallverdu, Raquel A. Martinez Garcia de la Torre, Inge Mannaerts, Stefaan Verhulst, Ayla Smout, Mar Coll, Silvia Arino, Teresa Rubio-Tomas, Beatriz Aguilar-Bravo, Celia Martinez-Sanchez, Delia Blaya, Catherine M. Verfaillie, Leo A. van Grunsven, Pau Sancho-Bru
Summary: Human iPSCs are differentiated into HSCs with growth factors for in vitro modeling. The protocol yields iPSC-HSCs with phenotypic and functional characteristics of primary HSCs, suitable for high-throughput in vitro studies. Coculturing iPSC-HSCs with hepatocytes allows for the formation of 3D hepatic spheroids, enabling modeling and drug screening studies.
Review
Biochemistry & Molecular Biology
Tianyuan Shi, Martin Cheung
Summary: Using rodents for modeling neurological diseases has limitations due to differences in genetics, neural development, and physiology between humans and rodents. In the past decade, induced pluripotent stem cells and induced neural stem cells generated through reprogramming somatic cells have offered a powerful alternative for studying disease pathogenesis and testing regenerative medicines. Urine-derived stem cells are an ideal cell source for reprogramming due to their proliferation, multipotency, epithelial nature, and ease of reprogramming.
CELL AND BIOSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Yue Su, Ling Wang, Zhiqiang Fan, Ying Liu, Jiaqi Zhu, Deborah Kaback, Julia Oudiz, Tayler Patrick, Siu Pok Yee, Xiuchun (Cindy) Tian, Irina Polejaeva, Young Tang
Summary: This study successfully generated biPSCs with long-term self-renewal capacity and pluripotent characteristics from bovine mesenchymal stem cells. The cells exhibited morphological changes under different culture conditions, and transcriptome analysis showed high similarity to bovine ESCs and embryos. The naive-like biPSCs also demonstrated the ability to integrate into extra-embryonic tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Hong Cao, Qian Zhou, Chungeng Liu, Yecen Zhang, Minghui Xie, Weihua Qiao, Nianguo Dong
Summary: This study investigates the effect of substrate stiffness on the differentiation of human-induced pluripotent stem cells (h-iPSCs) into valve endothelial-like cells (h-iVECs). The results show that moderate substrate stiffness can enhance the differentiation efficiency of h-iVECs and is regulated by the WNT/Calcineurin signaling pathway. This study emphasizes the importance of using substrate stiffness to achieve a more specific and mature differentiation of h-iVECs.
ACTA BIOMATERIALIA
(2022)
Article
Cardiac & Cardiovascular Systems
Parvin Forghani, Aysha Rashid, Fangxu Sun, Rui Liu, Dong Li, Megan R. Lee, Hyun Hwang, Joshua T. Maxwell, Anant Mandawat, Ronghu Wu, Khalid Salaita, Chunhui Xu
Summary: Anti-cancer therapies have improved patient outcomes, but cardiac side effects from these treatments are still a significant challenge. This study investigated the mechanisms underlying cardiotoxicity induced by the proteasome inhibitor carfilzomib using hiPSC-CMs. The findings showed that carfilzomib treatment caused deleterious changes in cellular and functional characteristics of hiPSC-CMs, affecting mitochondrial function and contractility. Insights into these changes were gained from omic analyses of gene and protein expression.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2021)
Article
Biochemistry & Molecular Biology
Ana Artero-Castro, Kathleen Long, Andrew Bassett, Almudena Avila-Fernandez, Marta Corton, Antonio Vidal-Puig, Pavla Jendelova, Francisco Javier Rodriguez-Jimenez, Eleonora Clemente, Carmen Ayuso, Slaven Erceg
Summary: Hereditary retinal dystrophies are a significant cause of blindness due to lack of effective treatments. Patient-derived human-induced pluripotent stem cells can potentially provide autologous cells for therapy, but the presence of gene mutations remains a challenge.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Meili Chen, Yibo Hu, Jingxing Liu, Qi Wu, Chenglin Zhang, Jun Yu, Jingfa Xiao, Fuwen Wei, Jiayan Wu
SCIENTIFIC REPORTS
(2015)
Article
Plant Sciences
Yongbing Zhao, Jinlong Yin, Haiyan Guo, Yuyu Zhang, Wen Xiao, Chen Sun, Jiayan Wu, Xiaobo Qu, Jun Yu, Xumin Wang, Jingfa Xiao
FRONTIERS IN PLANT SCIENCE
(2015)
Review
Genetics & Heredity
Jingfa Xiao, Zhewen Zhang, Jiayan Wu, Jun Yu
GENOMICS PROTEOMICS & BIOINFORMATICS
(2015)
Article
Microbiology
Guoxiang Shi, Mingming Su, Junrong Liang, Ran Duan, Wenpeng Gu, Yuchun Xiao, Zhewen Zhang, Haiyan Qiu, Zheng Zhang, Yi Li, Xiaohe Zhang, Yunchao Ling, Lai Song, Meili Chen, Yongbing Zhao, Jiayan Wu, Huaiqi Jing, Jingfa Xiao, Xin Wang
ARCHIVES OF MICROBIOLOGY
(2016)
Article
Biochemical Research Methods
Xin Sheng, Jiayan Wu, Qianqian Sun, Xue Li, Feng Xian, Manman Sun, Wan Fang, Meili Chen, Jun Yu, Jingfa Xiao
BRIEFINGS IN BIOINFORMATICS
(2017)
Article
Microbiology
Xinpeng Tian, Zhewen Zhang, Tingting Yang, Meili Chen, Jie Li, Fei Chen, Jin Yang, Wenjie Li, Bing Zhang, Zhang Zhang, Jiayan Wu, Changsheng Zhang, Lijuan Long, Jingfa Xiao
FRONTIERS IN MICROBIOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Yongbing Zhao, Chen Sun, Dongyu Zhao, Yadong Zhang, Yang You, Xinmiao Jia, Junhui Yang, Lingping Wang, Jinyue Wang, Haohuan Fu, Yu Kang, Fei Chen, Jun Yu, Jiayan Wu, Jingfa Xiao
Article
Microbiology
Xinyu Chen, Yadong Zhang, Zhewen Zhang, Yongbing Zhao, Chen Sun, Ming Yang, Jinyue Wang, Qian Liu, Baohua Zhang, Meili Chen, Jun Yu, Jiayan Wu, Zhong Jin, Jingfa Xiao
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Yongbing Zhao, Jinyue Wang, Fang Liang, Yanxia Liu, Qi Wang, Hao Zhang, Meiye Jiang, Zhewen Zhang, Wenming Zhao, Yiming Bao, Zhang Zhang, Jiayan Wu, Yan W. Asmann, Rujiao Li, Jingfa Xiao
NUCLEIC ACIDS RESEARCH
(2019)
Article
Biotechnology & Applied Microbiology
Yun Shu, Hui Li, Hongjuan Shang, Jun Chen, Xiaoxing Su, Wei Le, Yan Lei, Liming Tao, Cailiang Zou, Wendy Wu
ONCOTARGETS AND THERAPY
(2020)
Article
Oncology
ZeWen Xiao, Wendy Wu, Chunlong Wu, Man Li, Fuming Sun, Lu Zheng, Gaojing Liu, Xiaoling Li, Zhiyuan Yun, Jiebing Tang, Yang Yu, Shengnan Luo, Wenji Sun, Xiaohong Feng, Qian Cheng, Xue Tao, Shuangxiu Wu, Ji Tao
Summary: This study revealed distinct 5hmC signatures in cfDNA of CRC and AD patients compared to healthy controls, potentially aiding in early-stage CRC screening and assessing adenoma malignancy. The findings imply the importance of 5hmC modifications for different pathological subtypes in colorectal diseases, suggesting the need for further large-scale follow-up studies.
MOLECULAR ONCOLOGY
(2021)
Article
Oncology
Wei Zhang, Wenjuan Han, Bo Yu, Xin Zhao, Gaojun Lu, Wendy Wu, Yi Zhang
Summary: Gene mutations in primary and metastatic lesions of NSCLC patients were examined, revealing EGFR gene mutation as the most common with EGFR amplification more prevalent in the metastatic group. Metastatic group showed higher mutant-allele tumor heterogeneity value and shorter progression-free survival than the non-metastatic group.
Article
Biotechnology & Applied Microbiology
YunYu Lin, Yan Lei, LinWei Li, Xiaoxing Su, Qiqi Tian, Wendy Wu
Summary: This study identified a novel BAIAP2-ROS1 rearrangement and provided more knowledge of ROS1 fusion in clinical personalized treatment. The good response to crizotinib therapy emphasizes the importance of DNA-based and RNA-based NGS in rare fusion identification in clinical practice.
ONCOTARGETS AND THERAPY
(2022)
Review
Cell Biology
Wenjuan Zhang, Jian Cao, Ke Liu, Ziwei Qu, Ying Zheng, Jun Yu, Yishan Yu, Yongsheng Wang, Wendy Wu
Summary: Immunotherapy has become the main systemic therapy for cancer patients, but current biomarkers are limited by tumor tissue accessibility. Rare mutations in CD274 gene's UTR region have been suggested as potential biomarkers for immunotherapy efficacy. This study explores the multi-omics analysis of CD274 UTR region using cSMART 2.0 technology and finds that rare structural variants exist in different Chinese cancer populations, which can predict response to immune checkpoint inhibitors. Liquid biopsy-based cSMART 2.0 technology offers a more sensitive and accurate detection method when tissue samples are unavailable.
CELL DEATH DISCOVERY
(2023)
Article
Biotechnology & Applied Microbiology
Jun Jin, Rujiao Li, Chunlai Jiang, Ruosi Zhang, Xiaomeng Ge, Fang Liang, Xin Sheng, Wenwen Dai, Meili Chen, Jiayan Wu, Jingfa Xiao, Weiheng Su