Journal
ANALYTICAL CHEMISTRY
Volume 87, Issue 15, Pages 7610-7617Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.5b00640
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Funding
- National Natural Science Foundation of China [21275050, 21145001]
- Hunan Provincial Natural Science Foundation of China [13JJ1016]
- Scientific Research Fund of Hunan Provincial Education Department [13A053]
- Construct Program of the Key Discipline in Hunan Province
- Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province
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Carbon nanotubes (CNTs) labeled with aptamer and horseradish peroxidase (HRP) were used as a probe to amplify the impedimetric sensing of the aptamerprotein (with thrombin as the model) interaction. The HRP-biocatalyzed oxidation of 3,3-diaminobenzidine (DAB) in the presence of H2O2 and the postelectropolymerization of insoluble precipitates produced on the electrode supports were used as a signal amplification route for the sensing process. Thrombin was sensed by aptamer 1 immobilized on a glassy carbon electrode. The multiwalled CNT-aptamer 2-HRP probe was linked to the aptamer 1thrombin complex through the thrombinaptamer 2 interaction. The postelectropolymerization of biocatalyzed precipitates of DAB on the electrode greatly increased the electron-transfer resistance at the electrode-solution interface. Cyclic voltammetry and electrochemical impedance spectroscopy were employed to follow the stepwise fabrication of the aptasensor and impedimetric detection of thrombin. Thrombin concentration as low as 0.05 pM could be detected by this method. In addition, the proposed impedimetric aptasensor exhibits good sensitivity (5195 Omega decade(-1)), selectivity, and reproducibility. The aptasensor also has acceptable recovery for thrombin detection in complex protein sample.
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