Article
Genetics & Heredity
Hassan Dastsooz, Ahad Alizadeh, Parham Habibzadeh, Ali Nariman, Asieh Hosseini, Yaser Mansoori, Hamed Haghi-Aminjan
Summary: Our study provides insights into the ceRNA networks of TCGA-GI cancers, revealing common and distinct mRNA and lncRNA with prognostic values. Furthermore, we identified several genes, including KDELC1, that could act as novel proto-oncogenes in cancers and serve as potential prognostic and therapeutic targets. Additionally, we found cell cycle and extracellular matrix structural constituent as the top shared KEGG and molecular function, respectively, among GI cancers.
FRONTIERS IN GENETICS
(2022)
Article
Virology
Margarita Valdes Aleman, Luca D. Bertzbach, Thomas Speiseder, Wing Hang Ip, Ramon A. Gonzalez, Thomas Dobner
Summary: During human adenovirus infection, the late phase of viral replication results in a significant increase in viral late mRNA abundance, which outcompetes cellular mRNA biogenesis rather than selectively exporting viral mRNA.
Review
Surgery
Teng Cai, Lawrence Lawer Atteh, Xianzhuo Zhang, Chongfei Huang, Mingzhen Bai, Haidong Ma, Chao Zhang, Wenkang Fu, Long Gao, Yanyan Lin, Wenbo Meng
Summary: m6A modification is the most abundant internal modification of mRNA in higher eukaryotes, with dynamic and reversible effects. It plays important roles in both gastrointestinal tumors and non-tumor diseases, offering new insights into tumor treatment.
FRONTIERS IN SURGERY
(2022)
Article
Critical Care Medicine
Richi Nakatake, Tetsuya Okuyama, Masaya Kotsuka, Morihiko Ishizaki, Hiroaki Kitade, Katsuhiko Yoshizawa, Rene H. Tolba, Mikio Nishizawa, Mitsugu Sekimoto
Summary: Sepsis after major liver surgery is a critical issue due to the overproduction of nitric oxide (NO) in hepatocytes and macrophages. This study shows that a single-stranded oligonucleotide called SO1 can inhibit the interaction between iNOS mRNA and AS transcripts, leading to a reduction in iNOS mRNA levels.
Article
Engineering, Biomedical
Julius Sundermann, Sarah Oehmichen, Steffen Sydow, Laura Burmeister, Bastian Quaas, Robert Haensch, Ursula Rinas, Andrea Hoffmann, Henning Menzel, Heike Bunjes
Summary: Different surface modifications of PCL fiber mats influence the binding and release of BMP-2, with polydopamine coating showing the highest binding capacity. Functionalization with chitosan nanogel considerably increases BMP-2 binding on all hydrophilized surfaces and results in sustained release without loss of bioactivity.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Biochemistry & Molecular Biology
Colin F. Davenport, Tobias Scheithauer, Alessia Dunst, Frauke Sophie Bahr, Marie Dorda, Lutz Wiehlmann, Doan Duy Hai Tran
Summary: Downregulation of multiple tumor suppressor genes is crucial in cancer formation, with epigenetic modifications playing a significant role. Using nanopore sequencing technology, the study identified potential TSGs in HCC and demonstrated the tumor suppressor function of GCK in inhibiting HCC cell proliferation through lactate accumulation-induced energy crisis. These findings offer valuable insights for further understanding tumorigenesis in human cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Sebastian Burbano De Lara, Doan Duy Hai Tran, Aldrige Bernardus Allister, Mareike Polenkowski, Bjoern Nashan, Martina Koch, Teruko Tamura
Summary: This study identifies C20orf204-189AA as a cancer type-specific fine tuner in HCC, enhancing cell proliferation and ribosomal RNA transcription, and interacting with ribosomal RNA and nucleolin. The expression of C20orf204-189AA correlates with tumor differentiation grade and patient survival in HCC.
Article
Pharmacology & Pharmacy
Julius Sundermann, Steffen Sydow, Laura Burmeister, Andrea Hoffmann, Henning Menzel, Heike Bunjes
Summary: This study investigated the release performance of BMP-2 from PCL fiber mat-based implant prototypes coated with chitosan nanogel, highlighting the significant effects of chitosan and solubilizing additives on the recovery rate of BMP-2 in in vitro release experiments.
Article
Cell Biology
Beatrice Oelze, Kirsten Elger, Patrik Schadzek, Laura Burmeister, Anika Hamm, Sandra Laggies, Virginia Seiffart, Gerhard Gross, Andrea Hoffmann
Summary: The research suggests that the inflammatory mediator TAK1 in LPS-treated murine MSCs may have anti-inflammatory properties through the regulation of IL-6 expression.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Article
Biochemical Research Methods
Carla Lizbeth Segovia-Trinidad, Bastian Quaas, Zhaopeng Li, Antonina Lavrentieva, Yvonne Roger, Thomas Scheper, Andrea Hoffmann, Ursula Rinas
Summary: Smad8 is a transcriptional regulator in the TGF-beta signaling pathway, with cSmad8 showing constitutive activity and tendon cell inducing potential. By engineering cSmad8 to include the HIV TAT signal, researchers successfully achieved cellular internalization and demonstrated biological activity in C3H10T1/2 cells.
PROTEIN EXPRESSION AND PURIFICATION
(2021)
Review
Pathology
Christof A. Bertram, Nikolas Stathonikos, Taryn A. Donovan, Alexander Bartel, Andrea Fuchs-Baumgartinger, Karoline Lipnik, Paul J. van Diest, Federico Bonsembiante, Robert Klopfleisch
Summary: Digital microscopy is increasingly used in pathology for routine diagnostic and research work, with validation studies showing high reliability. However, individual laboratories are encouraged to conduct their own validation studies to ensure expected performance in their specific clinical or research setting.
VETERINARY PATHOLOGY
(2022)
Article
Veterinary Sciences
Nicole Schulze, Natasha Werpy, Jennifer Gernhardt, Guido Fritsch, Thomas Hildebrandt, Katrien Vanderperren, Robert Klopfleisch, Racem Ben Romdhane, Christophorus Lischer, Anna Ehrle
Summary: This study investigated the feasibility of using dynamic CT for diagnostic imaging of equine cervical articular process joints (APJs) and provided a detailed description of the APJ movement pattern. The results showed that the image quality of dynamic 3D videos was significantly lower than 2D videos, and the translational displacement of the APJ surface was greater in flexion movement than extension movement.
EQUINE VETERINARY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Mareike Polenkowski, Sebastian Burbano de Lara, Aldrige Bernardus Allister, Thi Nhu Quynh Nguyen, Teruko Tamura, Doan Duy Hai Tran
Summary: Identification of cancer-specific lncRNAs and micropeptides can contribute to novel treatment and immunotherapeutic strategies. In this study, we found a HCC-specific lncRNA encoding a micropeptide that enhances transcription of ribosomal RNA and supports growth of HCC. We also discovered novel micropeptides originating from HCC-specific lncRNAs. One of these micropeptides, Linc013026-68AA, is expressed in a subset of HCC cells and plays a role in cell proliferation. Our findings shed light on the importance of the "dark proteome" in cancer maintenance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Christian Marzahl, Jenny Hill, Jason Stayt, Dorothee Bienzle, Lutz Welker, Frauke Wilm, Joern Voigt, Marc Aubreville, Andreas Maier, Robert Klopfleisch, Katharina Breininger, Christof A. Bertram
Summary: This study introduces a novel, fully annotated multi-species P-Hem dataset, and develops an annotation pipeline using deep learning and data visualization techniques. The dataset is one of the largest publicly available WSIs datasets in terms of the number of annotations, scanned area, and species covered.
Article
Multidisciplinary Sciences
Frauke Wilm, Marco Fragoso, Christian Marzahl, Jingna Qiu, Chloe Puget, Laura Diehl, Christof A. Bertram, Robert Klopfleisch, Andreas Maier, Katharina Breininger, Marc Aubreville
Summary: The study presents a dataset consisting of 350 whole slide images and 12,424 polygon annotations for studying histological classification of canine cutaneous tumors. The provided labels show high consistency, and the dataset is validated by training a deep neural network for tissue segmentation and tumor subtype classification.
Article
Pathology
Elena Riccardi, Robert Klopfleish, Frazer Bell, Sophie Le Calvez, Louise J. Dawson
Summary: This study investigated the expression of MUM1 antigen in canine peripheral T-cell lymphoma (PTCL) and diffuse large B cell lymphoma (DLBCL). The results showed that MUM1 antigen was expressed in both PTCL and DLBCL. These findings suggest that a subset of neoplastic T and B lymphocytes can express MUM1. Further research is needed to understand the role of MUM1 in the biological behavior and prognosis of canine lymphoma.
VETERINARY PATHOLOGY
(2023)
Article
Pharmacology & Pharmacy
Leonie Berten-Schunk, Yvonne Roger, Heike Bunjes, Andrea Hoffmann
Summary: The design of implants for tissue transitions is challenging, and the rotator cuff in the shoulder is an example of such a transition. Electrospun fiber mats of PCL loaded with CS/TPP nanoparticles and TGF-beta(3) were used to optimize the implant for enthesis regeneration. Higher loading concentrations of TGF-beta(3) led to increased release, larger cell pellets, and enhanced chondrogenic differentiation of MSCs.
Article
Multidisciplinary Sciences
Mareike Polenkowski, Aldrige Bernardus Allister, Sebastian Burbano de Lara, Andrew Pierce, Bethany Geary, Omar El Bounkari, Lutz Wiehlmann, Andrea Hoffmann, Anthony D. Whetton, Teruko Tamura, Doan Duy Hai Tran
Summary: THOC5 is essential for the 3'processing of inducible genes, mRNA export, and stem cell survival. Depletion of THOC5 leads to altered mRNA 3'cleavage and changes in RNA polymerase II binding across genes. THOC5 is recruited near high-density polymerase II sites, suggesting its involvement in transcriptional elongation.
Article
Engineering, Biomedical
Merle Kempfert, Elmar Willbold, Sebastian Loewner, Cornelia Blume, Johannes Pitts, Henning Menzel, Yvonne Roger, Andrea Hoffmann, Nina Angrisani, Janin Reifenrath
Summary: Chronic tendon ruptures are common in orthopedics and the use of traditional surgical methods with implants is necessary. The study demonstrated the potential of 3D-printed PCL scaffolds as suitable candidates for tendon implants, with enhanced cytocompatibility achieved through surface modifications.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2022)