Article
Cell & Tissue Engineering
Mengfei Zhang, Na Li, Wenqing Liu, Xiaomin Du, Yudong Wei, Donghui Yang, Zhe Zhou, Fanglin Ma, Sha Peng, Shiqiang Zhang, Xin He, Chunling Bai, Guangpeng Li, Jinlian Hua
Summary: The study revealed an important role of Eif2s3y in dairy goat reproduction, promoting the proliferation of spermatogonial stem cells by activating the ERK signaling pathway and generating more colonies in infertile mice. This finding provides a crucial basis for future research on male spermatogenic cell restoration.
STEM CELLS INTERNATIONAL
(2021)
Review
Cell & Tissue Engineering
Kai Song, Maryam Farzaneh
Summary: Breast cancer stem cells play a critical role in the metastasis and aggressiveness of breast cancer, making them a suitable target for treatment. Signaling pathways such as Wnt, NF kappa B, and miRNAs are key players in regulating BCSC self-renewal and metastasis. Targeting these pathways may offer a novel therapeutic approach for breast cancer diagnosis and therapy.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Biochemistry & Molecular Biology
Chiara Mazziotta, Carmen Lanzillotti, Maria Rosa Iaquinta, Francesca Taraballi, Elena Torreggiani, John Charles Rotondo, Lucia Oton-Gonzalez, Elisa Mazzoni, Francesca Frontini, Ilaria Bononi, Monica De Mattei, Mauro Tognon, Fernanda Martini
Summary: MSCs are important source for bone regeneration and play a crucial role in osteogenic differentiation. MiRNAs regulate gene expression in osteogenic differentiation of MSCs and are involved in controlling important signaling pathways such as TGF-beta/BMP and Wnt/beta-catenin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Ava Nasrolahi, Shirin Azizidoost, Klaudia Radoszkiewicz, Sajad Najafi, Farhoodeh Ghaedrahmati, Omid Anbiyaee, Seyed Esmaeil Khoshnam, Maryam Farzaneh, Shahab Uddin
Summary: Glioma is a common malignant brain tumor that arises from glial tissue. Glioma cancer stem cells (GCSCs) have been identified as playing important roles in tumor initiation and metastasis. Targeting GCSCs and their associated signaling pathways, as well as non-coding RNAs, could be a novel strategy for glioma treatment. In this review, critical signaling pathways involved in GCSC tumorigenesis and invasiveness are summarized.
CELLULAR SIGNALLING
(2023)
Review
Cell Biology
Ekaterina Sergeevna Novoseletskaya, Pavel Vladimirovich Evdokimov, Anastasia Yurievna Efimenko
Summary: The extracellular matrix plays a crucial role in regulating cell behavior and fate in stem cell microenvironments. Different types of stem cells possess molecules that interact with the extracellular matrix and cause changes in gene expression. Signals from the extracellular matrix are important for embryonic and postnatal development, as well as tissue renewal and repair. Various methods are used to study the cellular signaling networks from the extracellular matrix and predict the pathways in target cells.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Biophysics
Thais Moraes-Lacerda, Marcelo Bispo de Jesus
Summary: Solid lipid nanoparticles (SLN) are widely used in various fields and show promising results in delivering biomolecules. However, the potential cytotoxic effects of SLN have been overlooked, and there is a lack of research in this area. This critical review emphasizes the importance of testing the biocompatibility and cytotoxicity of empty SLN and calls for further investigation on SLN toxicity, particularly in biomedical applications.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Review
Emergency Medicine
Xiaoxiang Wang, Yinghui Liu, Jia He, Jingru Wang, Xiaodong Chen, Ronghua Yang
Summary: This article reviews the regulation of hair follicles, HFSCs and related signaling pathways, summarizes previous research results, and reveals the regulatory mechanisms of HFSC proliferation and differentiation.
Article
Cell Biology
Xiaomin Du, Siyu Wu, Yudong Wei, Xiuwei Yu, Fanglin Ma, Yuanxin Zhai, Donghui Yang, Mengfei Zhang, Wenqing Liu, Haijing Zhu, Jiang Wu, Mingzhi Liao, Na Li, Chunling Bai, Guangpeng Li, Jinlian Hua
Summary: The study identified a subset of spermatogonial stem cells in dairy goats with high PAX7 expression, indicating that PAX7 can maintain the self-renewal of these cells.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jun Yu, Cong Shen, Meng Lin, Xia Chen, Xiuliang Dai, Zhiran Li, Yunhao Wu, Yangbo Fu, Jinxing Lv, Xiaoyan Huang, Bo Zheng, Fei Sun
Summary: In this study, we identified BMI1 as a key regulator of spermatogonial stem cell (SSC) maintenance. BMI1 represses target genes by increasing H2AK119ub and reducing H3K4me3 in SSCs. Additionally, BMI1 inhibition activates Wnt signaling and promotes nuclear translocation of beta-catenin in SSCs. Suppression of Wnt/beta-catenin signaling restores SSC maintenance in BMI1-deficient SSCs. Our findings not only reveal a novel mechanism for BMI1 in SSC maintenance, but also provide a potential strategy for treating male infertility.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Review
Oncology
Lei Wang, Zeng Jin, Rohan P. Master, Chandra K. Maharjan, Madison E. Carelock, Tiffany B. A. Reccoppa, Myung-Chul Kim, Ryan Kolb, Weizhou Zhang
Summary: This article summarizes the important features of breast cancer stem cells, major signaling pathways, and cellular interactions within the tumor microenvironment, highlighting the significance of BCSCs in breast cancer and potential therapeutic strategies.
Article
Cell & Tissue Engineering
Sidse Ehnnsen, Henrik J. Ditzel
Summary: Breast cancer stem cells play a crucial role in cancer initiation, therapy resistance, and progression, with recent research focusing on cellular signaling pathways such as the WNT pathway, NF-κB pathway, and cholesterol biosynthesis pathway. Targeting these pathways in combination with standard treatments may improve the prognosis of breast cancer patients.
Review
Endocrinology & Metabolism
Lei Diao, Paul J. Turek, Constance M. John, Fang Fang, Renee A. Reijo Pera
Summary: Spermatogonial stem cells (SSCs) are essential adult stem cells involved in spermatogenesis and male fertility. Understanding the regulation of SSCs is crucial for studying their function and the causes of male infertility, as well as developing new treatments through in vitro culture and differentiation of SSCs. This review highlights the recent research progress on SSC regulation and discusses the potential application of SSCs in fertility restoration.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Cell Biology
Alejandro Ordaz-Ramos, Olivia Tellez-Jimenez, Karla Vazquez-Santillan
Summary: Breast cancer stem cells (BCSCs) are a specific subpopulation of cells capable of self-renewal and differentiation into diverse tumor cells. The involvement of cancer stem cells in treatment resistance and cancer recurrence is well established. Specific signaling pathways, such as Wnt/beta-catenin, NF-kappa B, Notch, Hedgehog, TGF-beta, and Hippo, play crucial roles in regulating the self-renewal ability of cancer stem cells. Targeting these signaling pathways holds promise as a therapeutic strategy for combating breast cancer aggressiveness. This review discusses the main signaling pathways involved in maintaining the self-renewal ability of BCSCs and highlights current strategies to disrupt stemness-related signaling molecules.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Genetics & Heredity
Cai Hu, Qisheng Zuo, Kai Jin, Zongyi Zhao, Yuhui Wu, Jichang Gao, Chaoyong Wang, Yingjie Wang, Wanda Zhan, Jing Zhou, Fufu Cheng, Hongyan Sun, Yingjie Niu, Yani Zhang
Summary: This study reveals the crucial role of RA in promoting the formation of chicken spermatogonial stem cells through regulating the ECM-receptor interaction signaling pathway.
Article
Medicine, Research & Experimental
Fatemeh Sokouti Nasimi, Saber Zahri, Shahin Ahmadian, Afsaneh Bagherzadeh, Nahideh Nazdikbin Yamchi, Leila Haghighi, Alberto Miranda Bedate, Balal Khalilzadeh, Reza Rahbarghazi, Mahdi Mahdipour
Summary: The study showed that estradiol can increase the viability of mouse SSCs in a dose-dependent manner, leading to changes in cell morphology and gene expression, as well as reduced sperm parameters and testicular progenitor cells. This suggests that estradiol may induce Sertoli cell differentiation of SSCs while long-term exogenous administration can lead to infertility.
Article
Microbiology
Soon-Hwan Oh, Allyson Isenhower, Rubi Rodriguez-Bobadilla, Brooke Smith, Jillian Jones, Vit Hubka, Christopher Fields, Alvaro Hernandez, Lois L. Hoyer
Summary: The ALS gene family encodes adhesins that promote colonization by opportunistic fungal pathogens such as Candida tropicalis. Long-read DNA sequencing technology was used to characterize ALS loci in C. tropicalis, providing insight for future experimentation on adhesion and pathogenesis.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Carl J. Yeoman, Christopher J. Fields, Pascale Lepercq, Philippe Ruiz, Evelyne Forano, Bryan A. White, Pascale Mosoni
Summary: This study provides the first evidence of in vivo competitions between F. succinogenes and the two Ruminococcus species. It shows that a simple disequilibrium within the cellulolytic community has repercussions on the rumen metabolome and fermentation end products. This finding will have to be considered in the future when determining strategies aiming at directing rumen fermentations for animal production.
Article
Biochemical Research Methods
Verena Ras, Gerrit Botha, Shaun Aron, Katie Lennard, Imane Allali, Shantelle Claassen-Weitz, Kilaza Samson Mwaikono, Dane Kennedy, Jessica R. Holmes, Gloria Rendon, Sumir Panji, Christopher J. Fields, Nicola Mulder
Summary: By extending the remote classroom model and utilizing containerized workflows, H3ABioNet successfully developed a 16S rRNA Microbiome Intermediate Bioinformatics Training course to enhance the knowledge and skills of African-based research groups in microbiome studies and data analysis. This approach allowed for a standard replicable software stack deployment across different hosting sites, enabling participants to replicate analyses and results on real data post-training.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Editorial Material
Andrology
Marie-Claude Hofmann, Aleksander Giwercman
Editorial Material
Andrology
Marie-Claude Hofmann, Aleksander Giwercman
Review
Endocrinology & Metabolism
Marie-Claude Hofmann, Elena McBeath
Summary: This review focuses on the activation of master regulators of the niche in Sertoli cells and their targets, as well as novel molecular mechanisms underlying the regulation of growth and differentiation factors through NOTCH signaling and other pathways.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Oncology
Marie-Claude Hofmann, Muthusamy Kunnimalaiyaan, Jennifer R. Wang, Naifa L. Busaidy, Steven Sherman, Stephen Y. Lai, Mark Zafereo, Maria E. Cabanillas
Summary: Protein kinases play critical roles in cell survival, proliferation, and motility, and their dysregulation is common in solid tumors like thyroid cancer. Inhibiting activated protein kinases has been a breakthrough in thyroid cancer therapy, but drug resistance remains a challenge that needs to be overcome with new strategies.
ENDOCRINE-RELATED CANCER
(2022)
Review
Obstetrics & Gynecology
Lars Bjorndahl, Christopher L. R. Barratt, David Mortimer, Ashok Agarwal, Robert J. Aitken, Juan G. Alvarez, Natalie Aneck-Hahn, Stefan Arver, Elisabetta Baldi, Lluis Bassas, Florence Boitrelle, Riana Bornman, Douglas T. Carrell, Jose A. Castilla, Gerardo Cerezo Parra, Jerome H. Check, Patricia S. Cuasnicu, Sally Perreault Darney, Christiaan de Jager, Christopher J. De Jonge, Joel R. Drevet, Erma Z. Drobnis, Stefan S. Du Plessis, Michael L. Eisenberg, Sandro C. Esteves, Evangelini A. Evgeni, Alberto Ferlin, Nicolas Garrido, Aleksander Giwercman, Ilse G. F. Goovaerts, Trine B. Haugen, Ralf Henkel, Lars Henningsohn, Marie-Claude Hofmann, James M. Hotaling, Piotr Jedrzejczak, Pierre Jouannet, Niels Jorgensen, Jackson C. Kirkman Brown, Csilla Krausz, Maciej Kurpisz, Ulrik Kvist, Dolores J. Lamb, Hagai Levine, Kate L. Loveland, Robert McLachlan, Ali Mahran, Liana Maree, Sarah Martins da Silva, Michael T. Mbizvo, Andreas Meinhardt, Roelof Menkveld, Sharon T. Mortimer, Sergey Moskovtsev, Charles H. Muller, Maria Jose Munuce, Monica Muratori, Craig Niederberger, Cristian O'Flaherty, Rafael Oliva, Willem Ombelet, Allan A. Pacey, Michael A. Palladino, Ranjith Ramasamy, Liliana Ramos, Nathalie Rives, Eduardo Rs Roldan, Susan Rothmann, Denny Sakkas, Andrea Salonia, Maria Cristina Sanchez-Pozo, Rosanna Sapiro, Stefan Schlatt, Peter N. Schlegel, Hans-Christian Schuppe, Rupin Shah, Niels E. Skakkebaek, Katja Teerds, Igor Toskin, Herman Tournaye, Paul J. Turek, Gerhard van der Horst, Monica Vazquez-Levin, Christina Wang, Alex Wetzels, Theodosia Zeginiadou, Armand Zini
Summary: Biomedical science is making rapid progress in terms of transparency, openness, and reproducibility of scientific publications, particularly in studies based on human semen examination. Two recent documents, the 6th edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen, and the International Standard ISO 23162:2021, provide guidelines for authors to follow these laboratory methods and use a checklist to ensure the reliability of their studies when publishing in peer-reviewed journals.
HUMAN REPRODUCTION
(2022)
Editorial Material
Andrology
Aleksander Giwercman, Marie Claude Hofmann
Article
Andrology
Ewa Rajpert-De Meyts, Douglas T. Carrell, Manuela Simoni, Aleksander Giwercman, Marie-Claude Hofmann
Review
Andrology
Robert John Aitken, Sheena E. M. Lewis
Summary: This review summarizes the causes and consequences of DNA damage in the male germ line, including how DNA integrity is maintained in stem cells and the increase in background mutation rates with paternal age. It explains the repair pathways activated in germ cells and spermatozoa in response to genetic damage, as well as the potential mutagenic effects of such damage on offspring. The strategies for managing high levels of DNA damage in spermatozoa to reduce risks to offspring health are also discussed.
Article
Oncology
Madison M. Rose, Veronica L. Espinoza, Katelyn J. Hoff, Laura A. Pike, Vibha Sharma, Marie-Claude Hofmann, Aik Choon Tan, Nikita Pozdeyev, Rebecca E. Schweppe
Summary: Thyroid cancer is a common malignancy with limited treatment options. Inhibition of Src and MAPK pathway can enhance anti-tumor effects, and BIM may serve as a potential biomarker of response.
Review
Biochemistry & Molecular Biology
Elena McBeath, Keigi Fujiwara, Marie-Claude Hofmann
Summary: Until recently, the methods for generating floxed mice have been challenging, expensive, error-prone, or time-consuming. To overcome these issues, some labs have successfully used a small artificial intron to conditionally knockout a gene of interest in mice. However, others have encountered difficulties with the technique, primarily due to incorrect splicing or insufficient knockout of the gene's protein. This article presents a guide on selecting the appropriate exon and placing the recombinase-regulated artificial intron to prevent splicing disruption and maximize mRNA degradation. Following these recommendations should increase the success rate of producing tissue-specific knockout mice using this alternative technique.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Xiao Zhao, Jennifer Rui Wang, Ramona Dadu, Naifa Lamki Busaidy, Lei Xu, Kim O. Learned, Noah N. Chasen, Thinh Vu, Anastasios Maniakas, Arturo A. Eguia, Julia Diersing, Neil D. Gross, Ryan Goepfert, Stephen Y. Lai, Marie-Claude Hofmann, Renata Ferrarotto, Charles Lu, Gary Brandon Gunn, Michael T. Spiotto, Vivek Subbiah, Michelle D. Williams, Maria E. Cabanillas, Mark E. Zafereo
Summary: This study aimed to describe the outcomes of patients with BRAF(V600E)-mutated ATC who received neoadjuvant BRAF-directed therapy followed by surgery. The results showed that neoadjuvant therapy reduced the extent of surgery and surgical morbidity, and patients treated with neoadjuvant therapy had higher overall survival and progression-free survival rates compared to those who didn't have surgery or had surgery before therapy.
Review
Endocrinology & Metabolism
Sarah Hamidi, Marie-Claude Hofmann, Priyanka C. C. Iyer, Maria E. E. Cabanillas, Mimi I. I. Hu, Naifa L. L. Busaidy, Ramona Dadu
Summary: The treatment of advanced, radioiodine refractory, differentiated thyroid cancers (RR-DTCs) has seen significant advancements in the past decade, leading to a paradigm shift in patient management and prognosis. Improved understanding of tumor molecular drivers and access to next generation sequencing have resulted in the development and FDA-approval of targeted therapies for RR-DTCs, including multikinase inhibitors and fusion-specific kinase inhibitors. However, current treatments are not curative, and research efforts are focused on identifying and overcoming resistance mechanisms to tyrosine kinase inhibitors.
FRONTIERS IN ENDOCRINOLOGY
(2023)
