Uncovering methods for the prevention of protein aggregation and improvement of product quality in a transient expression system

When less becomes more: Optimization of protein expression in HEK293–EBNA1 cells using plasmid titration – A case study for NLRs

(2014) Els F. Halff et al.   PROTEIN EXPRESSION AND PURIFICATION

Block decoys: Transcription-factor decoys designed for in vitro gene regulation studies

(2013) Adam J. Brown et al.   ANALYTICAL BIOCHEMISTRY

Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells

(2013) David L. Hacker et al.   PROTEIN EXPRESSION AND PURIFICATION

Russell body inducing threshold depends on the variable domain sequences of individual human IgG clones and the cellular protein homeostasis

(2012) Janelle Stoops et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells

(2012) Yashas Rajendra et al.   BIOTECHNOLOGY AND BIOENGINEERING

Identifying bottlenecks in transient and stable production of recombinant monoclonal-antibody sequence variants in chinese hamster ovary cells

(2012) Megan Mason et al.   BIOTECHNOLOGY PROGRESS

In VivoCrystallization of Human IgG in the Endoplasmic Reticulum of Engineered Chinese Hamster Ovary (CHO) Cells

(2011) Haruki Hasegawa et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Reversal of Cancer Cachexia and Muscle Wasting by ActRIIB Antagonism Leads to Prolonged Survival

(2010) Xiaolan Zhou et al.   CELL

Mild Hypothermia Improves Transient Gene Expression Yields Several Fold in Chinese Hamster Ovary Cells

(2008) S. Wulhfard et al.   BIOTECHNOLOGY PROGRESS

Proteomic Identification and Functional Validation of Activins and Bone Morphogenetic Protein 11 as Candidate Novel Muscle Mass Regulators

(2008) Tatyana A. Souza et al.   MOLECULAR ENDOCRINOLOGY

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

(2008) Gaurav Backliwal et al.   NUCLEIC ACIDS RESEARCH

Enhanced Production of Monomeric Interferon-β by CHO Cells through the Control of Culture Conditions

(2005) J. Rodriguez et al.   BIOTECHNOLOGY PROGRESS