Technology developments in biological tools for targeted genome surgery

Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity

(2014) John P Guilinger et al.   NATURE METHODS

Cell-Penetrating Peptide-Mediated Delivery of TALEN Proteins via Bioconjugation for Genome Engineering

(2014) Jia Liu et al.   PLoS One

Transcription activator-like effector nuclease-mediated transduction of exogenous gene into IL2RG locus

(2014) Yohei Matsubara et al.   Scientific Reports

Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems

(2014) Akihiro Yasue et al.   Scientific Reports

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

A single-chain TALEN architecture for genome engineering

(2013) Ning Sun et al.   Molecular BioSystems

Robust, synergistic regulation of human gene expression using TALE activators

(2013) Morgan L Maeder et al.   NATURE METHODS

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

Engineering nucleases for gene targeting: safety and regulatory considerations

(2013) Katia Pauwels et al.   New Biotechnology

TAL Effector Specificity for base 0 of the DNA Target Is Altered in a Complex, Effector- and Assay-Dependent Manner by Substitutions for the Tryptophan in Cryptic Repeat –1

(2013) Erin L. Doyle et al.   PLoS One

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Crystal structure of a TALE protein reveals an extended N-terminal DNA binding region

(2012) Haishan Gao et al.   CELL RESEARCH

Recognition of methylated DNA by TAL effectors

(2012) Dong Deng et al.   CELL RESEARCH

A TALEN Genome-Editing System for Generating Human Stem Cell-Based Disease Models

(2012) Qiurong Ding et al.   Cell Stem Cell

Advances in targeted genome editing

(2012) Pablo Perez-Pinera et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Overcoming Transcription Activator-like Effector (TALE) DNA Binding Domain Sensitivity to Cytosine Methylation

(2012) Julien Valton et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Tell Me a Tale of TALEs

(2012) Alejandra Muñoz Bodnar et al.   MOLECULAR BIOTECHNOLOGY

In vivo genome editing using a high-efficiency TALEN system

(2012) Victoria M. Bedell et al.   NATURE

High-efficiency TALEN-based gene editing produces disease-resistant rice

(2012) Ting Li et al.   NATURE BIOTECHNOLOGY

TAL effector RVD specificities and efficiencies

(2012) Jana Streubel et al.   NATURE BIOTECHNOLOGY

A ligation-independent cloning technique for high-throughput assembly of transcription activator–like effector genes

(2012) Jonathan L Schmid-Burgk et al.   NATURE BIOTECHNOLOGY

Rapid hierarchical assembly of medium-size DNA cassettes

(2012) Jonathan Leo Schmid-Burgk et al.   NUCLEIC ACIDS RESEARCH

Targeted transcriptional activation of silent oct4 pluripotency gene by combining designer TALEs and inhibition of epigenetic modifiers

(2012) Sebastian Bultmann et al.   NUCLEIC ACIDS RESEARCH

Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs

(2012) Lindsay Cade et al.   NUCLEIC ACIDS RESEARCH

Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers

(2012) Adrian W. Briggs et al.   NUCLEIC ACIDS RESEARCH

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient TALEN-mediated gene knockout in livestock

(2012) D. F. Carlson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target

(2012) A. N.-S. Mak et al.   SCIENCE

Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors

(2012) D. Deng et al.   SCIENCE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains

(2012) Le Cong et al.   Nature Communications

Conservation and divergence of DNA methylation in eukaryotes

(2011) Zhixi Su et al.   Epigenetics

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

Targeted gene disruption in somatic zebrafish cells using engineered TALENs

(2011) Jeffry D Sander et al.   NATURE BIOTECHNOLOGY

Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection

(2011) Vikram Pattanayak et al.   NATURE METHODS

Gene-editing nucleases

(2011) Monya Baker   NATURE METHODS

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

(2011) Tomas Cermak et al.   NUCLEIC ACIDS RESEARCH

Assembly of custom TALE-type DNA binding domains by modular cloning

(2011) Robert Morbitzer et al.   NUCLEIC ACIDS RESEARCH

A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity

(2011) Claudio Mussolino et al.   NUCLEIC ACIDS RESEARCH

Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein

(2011) Magdy M. Mahfouz et al.   PLANT MOLECULAR BIOLOGY

Assembly of Designer TAL Effectors by Golden Gate Cloning

(2011) Ernst Weber et al.   PLoS One

CRISPR/Cas System and Its Role in Phage-Bacteria Interactions

(2010) Hélène Deveau et al.   Annual Review of Microbiology

Zinc-finger nucleases: new strategies to target the rat genome

(2010) Aron M. Geurts et al.   CLINICAL SCIENCE

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE

Conserved role of intragenic DNA methylation in regulating alternative promoters

(2010) Alika K. Maunakea et al.   NATURE

A TALE nuclease architecture for efficient genome editing

(2010) Jeffrey C Miller et al.   NATURE BIOTECHNOLOGY

TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain

(2010) Ting Li et al.   NUCLEIC ACIDS RESEARCH

Regulation of selected genome loci using de novo-engineered transcription activator-like effector (TALE)-type transcription factors

(2010) R. Morbitzer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CRISPR/Cas, the Immune System of Bacteria and Archaea

(2010) P. Horvath et al.   SCIENCE

Homing endonucleases: from basics to therapeutic applications

(2009) Maria J. Marcaida et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

The type III effectors ofXanthomonas

(2009) FRANK F. WHITE et al.   MOLECULAR PLANT PATHOLOGY

Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

(2009) J. Boch et al.   SCIENCE

Unexpected failure rates for modular assembly of engineered zinc fingers

(2008) Cherie L Ramirez et al.   NATURE METHODS

Disruption of the CFTR Gene Produces a Model of Cystic Fibrosis in Newborn Pigs

(2008) C. S. Rogers et al.   SCIENCE

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE

CRISPR Interference Limits Horizontal Gene Transfer in Staphylococci by Targeting DNA

(2008) Luciano A. Marraffini et al.   SCIENCE