Article
Biotechnology & Applied Microbiology
Jun-feng Li, Hong-fang Li, Shu-min Yao, Meng-juan Zhao, Wen-xun Dong, Sheng-kang Liang, Xing-yong Xu
Summary: The study focused on enhancing the oxygen utilization efficiency and production of Sophorolipids by introducing the Vitreoscilla hemoglobin (VHb) gene into S. bombicola. The results showed improved SLs production in shake flask culture and a higher DO level in the fermentation broth in a 5 L bioreactor. VHb expression also upregulated key genes related to electron transfer, TCA cycle, and SL synthesis, leading to enhanced biosynthesis of SLs and intracellular oxygen utilization efficiency.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Adilson Jose da Silva, Josivan de Souza Cunha, Teri Hreha, Kelli Cristina Micocci, Heloisa Sobreiro Selistre-de-Araujo, Blanca Barquera, Mattheos A. G. Koffas
Summary: This study engineered the pyocyanin biosynthetic pathway for high production in a heterologous host for the first time. Optimized culture and induction parameters led to a 3.5-fold increase in pyocyanin accumulation, while pathway balancing through testing plasmids with different copy numbers resulted in a fourfold difference in product titer among the engineered strains. Co-expression of Vitreoscilla hemoglobin Vhb further improved pyocyanin production to a final titer of 18.8 mg/L, demonstrating the potential of using Escherichia coli for phenazines production in electro-fermentation systems.
METABOLIC ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Xiao-Ting Ding, Yong Fan, Er-Ying Jiang, Xiao-Yi Shi, Eike Krautter, Guang-Rong Hu, Fu-Li Li
Summary: This study successfully improved the growth rate and eicosapentaenoic acid content of N. oceanica cells by introducing the Vitreoscilla hemoglobin gene, while reducing ROS levels and photorespiration levels. These results indicate that Vitreoscilla hemoglobin can enhance microalgal growth under stress conditions by mitigating ROS damage and modulating photorespiration.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Agricultural Engineering
Yang Zhang, Xiuling Zhou, Yingying Yao, Qianqian Xu, Haiying Shi, Kuiming Wang, Wei Feng, Yanbing Shen
Summary: Coexpression of Vitreoscilla hemoglobin and sterol transporter ATPase genes in Mycobacterium sp. was found to improve androstenone production. Immobilization on bagasse increased productivity significantly.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Selvaraju Kanagarajan, Magnus L. R. Carlsson, Sandeep Chakane, Karin Kettisen, Emanuel Smeds, Ranjeet Kumar, Niklas Ortenlof, Magnus Gram, Bo Akerstrom, Leif Bulow, Li-Hua Zhu
Summary: This study successfully produced recombinantly fused endotoxin-free HbF in Nicotiana benthamiana through Agrobacterium tumefaciens-mediated transient expression, demonstrating its high potential for use as blood substitutes. The recombinant protein showed structural and functional properties similar to native HbF, confirmed by various analyses including SDS-PAGE, Western blot, and mass spectrometry.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Karim E. Jaen, Daniela Velazquez, Juan-Carlos Sigala, Alvaro R. Lara
Summary: This study demonstrated that expressing Vitreoscilla hemoglobin (VHb) in E. coli chromosome can improve plasmid DNA (pDNA) production in biphasic fed-batch cultures by reducing acetate accumulation and increasing pDNA yields. The presence of VHb also led to higher fluxes in anaplemtic pathways and increased lactate production in cells under microaembic conditions. Despite lower RNAII/RNAI ratio, VHb-expressing strain achieved a significantly higher final pDNA titer compared to the wild type strain.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Review
Microbiology
Dale A. Webster, Kanak L. Dikshit, Krishna R. Pagilla, Benjamin C. Stark
Summary: In 1986, the discovery of hemoglobin (VHb) in Vitreoscilla bacteria expanded the scope of oxygen binding proteins. Research in the following years focused on its biochemical properties, genetic sequence, and expression control, leading to practical applications in microbial hosts. VHb's pioneering role in bacterial hemoglobin research and its ongoing biotechnological applications have made it a topic of interest 35 years after its discovery.
Article
Microbiology
Zi-Xu Wang, Na Li, Jun-Wei Xu
Summary: Efficient expression of the Vitreoscilla hemoglobin gene in G. lucidum increased the production of exopolysaccharides and enhanced their antioxidant activity, without changing their monosaccharide composition or molecular weight distribution.
Article
Biotechnology & Applied Microbiology
Qing Zhang, Yaozhong Chen, Lin Gao, Jian'gang Chen, Xin Ma, Dongbo Cai, Dong Wang, Shouwen Chen
Summary: This study optimized the expression cassette of VHB for the production of gamma-PGA, resulting in increased yields and improved oxygen transfer and ATP generation. The study also identified a suitable promoter for VHB expression and gamma-PGA synthesis, leading to further increases in gamma-PGA yield. Overall, this study achieved an efficient VHB expression cassette for oxygen transfer and gamma-PGA synthesis, with potential applications in the production of other metabolites.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Article
Chemistry, Physical
Yana Duan, Zhanglin Hou, Yiwu Zong, Fangfu Ye, Kun Zhao
Summary: The research reveals that for systems with a concentrated arrested region, the ratio of n-merization increases with the increase of area fraction, eventually becoming the dominant reaction in the system; dynamic heterogeneity originates from the divergent size of clusters consisting of high-mobility particles; high-mobility particles have a higher ability to react with others at high area fractions, and binding reactions are spatially correlated with dynamic heterogeneity.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Article
Medicine, Research & Experimental
Qinzi Xu, Jason J. Rose, Xiukai Chen, Ling Wang, Anthony W. DeMartino, Matthew R. Dent, Sagarika Tiwari, Kaitlin Bocian, Xueyin N. Huang, Qin Tong, Charles F. McTiernan, Lanping Guo, Elmira Alipour, Trevor C. Jones, K. Burak Ucer, Daniel B. Kim-Shapiro, Jesus Tejero, Mark T. Gladwin
Summary: This study evaluates different hemoproteins and finds that 2,3-diphosphoglycerate stripped human hemoglobin (StHb) and N-ethylmaleimide modified hemoglobin (NEMHb) have high CO binding affinities and efficiently scavenge CO from erythrocytes, suggesting their potential therapeutic role in CO poisoning.
Article
Green & Sustainable Science & Technology
B. Miyawaki, A. B. Mariano, J. V. C. Vargas, W. Balmant, A. C. Defrancheschi, D. O. Correa, B. Santos, N. F. H. Selesu, J. C. Ordonez, V. M. Kava
Summary: In this study, microalgae were cultivated using different organic wastes and wastewater, and the results showed a significant impact of BSM on the growth of microalgae and bioenergy production. Microalgae were able to efficiently remove ammonia and phosphorus from BSM, degrade organic matter content, and significantly increase biomass and lipid content.
Article
Chemistry, Multidisciplinary
Mengshi Jiang, Bing Qin, Lihua Luo, Xiang Li, Yingying Shi, Junlei Zhang, Zhenyu Luo, Chunqi Zhu, Guannan Guan, Yongzhong Du, Jian You
Summary: The study compared three methods of alleviating tumor hypoxia in terms of biosafety, efficacy and clinical applicability, with Hb@lipo standing out as a promising oxygen carrier. The combination therapy with PD-1 antibody showed a distinct synergistic suppression effect on tumors with more M1 macrophages.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Chemistry, Multidisciplinary
Yanqin Song, Muhammad Ismail, Qi Shan, Jianing Zhao, Yanping Zhu, Leiming Zhang, Yuan Du, Longbing Ling
Summary: The study introduced a new liposome nanoformulation Dex@FA-ROS-Lips for precise treatment of rheumatoid arthritis, showing improved therapeutic effect and potential in targeting inflammatory diseases.
Article
Optics
Habibeh Pourhassan, Ebrahim Safari, Ayuob Aghanejad, Mohammad Reza Tohidkia
Summary: Surface plasmon resonance (SPR) biosensors are widely used in medicine and the pharmaceutical industry for studying and analyzing interactions between target molecules and biological detection. A new dissolved oxygen sensor based on SPR and Goos-Hanchen shift was proposed for sensitive detection of oxygen-dissolved hemoglobin.
OPTICS AND LASER TECHNOLOGY
(2022)
Article
Environmental Sciences
Laura Haak, Ratul Roy, Krishna Pagilla
Article
Biochemistry & Molecular Biology
Ratul Roy, Laura Haak, Lin Li, Krishna Pagilla
PROCESS BIOCHEMISTRY
(2016)
Article
Biotechnology & Applied Microbiology
Taner Sar, Benjamin C. Stark, Meltem Yesilcimen Akbas
Article
Construction & Building Technology
S. A. Kunkel, P. Azimi, H. Zhao, B. C. Stark, B. Stephens
Article
Cell Biology
Benjamin C. Stark, M. Hunter Lanier, John A. Cooper
MOLECULAR BIOLOGY OF THE CELL
(2017)
Review
Engineering, Environmental
Lin Li, Krishna R. Pagilla
Article
Multidisciplinary Sciences
Jia Wang, Robert R. Butler, Fan Wu, Jean-Francois Pombert, John J. Kilbane, Benjamin C. Stark
Article
Energy & Fuels
Taner Sar, Benjamin C. Stark, Meltem Yesilcimen Akbas
Summary: The study shows that the Vitreoscilla hemoglobin/immobilization system can enhance ethanol production, with strain TS3 producing higher yields compared to strain FBR5, and ethanol production increasing with lactose concentration.
Article
Biotechnology & Applied Microbiology
T. Sar, Y. Chen, Y. Bai, B. Liu, P. Agarwal, B. C. Stark, M. Y. Akbas
Summary: The enhancement of desulfurization activities of Paenibacillus strains 32O-W and 32O-Y was investigated using different sulfur sources, with the co-culture of strains showing the best growth under specific conditions.
LETTERS IN APPLIED MICROBIOLOGY
(2021)
Review
Microbiology
Dale A. Webster, Kanak L. Dikshit, Krishna R. Pagilla, Benjamin C. Stark
Summary: In 1986, the discovery of hemoglobin (VHb) in Vitreoscilla bacteria expanded the scope of oxygen binding proteins. Research in the following years focused on its biochemical properties, genetic sequence, and expression control, leading to practical applications in microbial hosts. VHb's pioneering role in bacterial hemoglobin research and its ongoing biotechnological applications have made it a topic of interest 35 years after its discovery.
Article
Developmental Biology
Benjamin C. Stark, Yuanyuan Gao, Diane S. Sepich, Lakyn Belk, Matthew A. Culver, Bo Hu, Marlene Mekel, Wyndham Ferris, Jimann Shin, Lilianna Solnica-Krezel, Fang Lin, John A. Cooper
Summary: Analysis of zebrafish carrying mutations of carmil3 revealed impaired endodermal and DFC migration during gastrulation, affecting KV morphogenesis. Mutant KVs displayed smaller size, reduced cell numbers, and decreased cilia, leading to defects in L/R patterning with varying penetrance and expressivity. These findings suggest a new role for CARMIL3 in vertebrate embryo morphogenesis, involving endodermal cell and DFC migration, as well as KV and L/R asymmetry formation.
DEVELOPMENTAL BIOLOGY
(2022)
Editorial Material
Microbiology
Benjamin C. Stark
Article
Biotechnology & Applied Microbiology
Taner Sar, Murat Ozturk, Benjamin C. Stark, Meltem Yesilcimen Akbas
Summary: This study investigated improvements in desulfurization of DBT and DBTS by Paenibacillus strains through immobilization and nanoparticle coating, showing promising results for enhancing biodesulfurization efficiency.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Tayebeh Goli, Kamrun Ahmadi, Krishna R. Pagilla, Eric A. Marchand
Summary: This study investigated the removal of dissolved organic nitrogen (DON) in reclaimed water through enhanced coagulation, ozonation, and ozonation/biodegradation at both lab and pilot scales. The results showed that DON could be effectively removed by enhanced coagulation, ozonation, and ozonation/biodegradation, with the best results achieved by the combination of ozonation and biodegradation.
JOURNAL OF WATER PROCESS ENGINEERING
(2023)
Meeting Abstract
Cell Biology
B. C. Stark, L. Solnica-Krezel, J. A. Cooper
MOLECULAR BIOLOGY OF THE CELL
(2017)
