PEG chain length impacts yield of solid-phase protein PEGylation and efficiency of PEGylated protein separation by ion-exchange chromatography: Insights of mechanistic models

Characterization of a Protein Conjugate Using an Asymmetrical-Flow Field-Flow Fractionation and a Size-Exclusion Chromatography with Multi-Detection System

(2012) Katja Rebolj et al.   ANALYTICAL CHEMISTRY

Microchip capillary gel electrophoresis of multiply PEGylated high-molecular-mass glycoproteins

(2012) Birgit K. Seyfried et al.   Biotechnology Journal

Isoform separation and binding site determination of mono-PEGylated lysozyme with pH gradient chromatography

(2012) Benjamin Maiser et al.   JOURNAL OF CHROMATOGRAPHY A

Mechanistic Insights into the Stabilization of srcSH3 by PEGylation

(2012) Wei Meng et al.   LANGMUIR

The Conformation of the Poly(ethylene glycol) Chain in Mono-PEGylated Lysozyme and Mono-PEGylated Human Growth Hormone

(2011) Sheetal S. Pai et al.   BIOCONJUGATE CHEMISTRY

Aldehyde PEGylation Kinetics: A Standard Protein versus a Pharmaceutically Relevant Single Chain Variable Fragment

(2011) Anna Moosmann et al.   BIOCONJUGATE CHEMISTRY

Integrated Solid-Phase Synthesis and Purification of PEGylated Protein

(2011) Xiaojiao Shang et al.   BIOMACROMOLECULES

Microcalorimetric study of the adsorption of PEGylated lysozyme on a strong cation exchange resin

(2011) Tim Blaschke et al.   JOURNAL OF CHROMATOGRAPHY A

Influence of protein and stationary phase properties on protein–matrix-interaction in cation exchange chromatography

(2011) Marina Urmann et al.   JOURNAL OF CHROMATOGRAPHY A

State of the art in PEGylation: The great versatility achieved after forty years of research

(2011) Gianfranco Pasut et al.   JOURNAL OF CONTROLLED RELEASE

An oriented adsorption strategy for efficient solid phase PEGylation of recombinant staphylokinase by immobilized metal-ion affinity chromatography

(2011) Jun Wang et al.   PROCESS BIOCHEMISTRY

Effects of Polymer Molecular Weight on the Size, Activity, and Stability of PEG-Functionalized Trypsin

(2010) Karen Chiu et al.   BIOMACROMOLECULES

Purification of singly PEGylated α-lactalbumin using charged ultrafiltration membranes

(2010) Krisada Ruanjaikaen et al.   BIOTECHNOLOGY AND BIOENGINEERING

Interaction mechanism of mono-PEGylated proteins in electrostatic interaction chromatography

(2010) Mitsuyo Abe et al.   Biotechnology Journal

Solubility and binding properties of PEGylated lysozyme derivatives with increasing molecular weight on hydrophobic-interaction chromatographic resins

(2010) Egbert Müller et al.   JOURNAL OF CHROMATOGRAPHY A

Purification of PEGylated Protein Using Membrane Chromatography

(2010) Deqiang Yu et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Protein PEGylation Attenuates Adsorption and Aggregation on a Negatively Charged and Moderately Hydrophobic Polymer Surface

(2010) Sheetal S. Pai et al.   LANGMUIR

Product development issues for PEGylated proteins

(2010) Robert W. Payne et al.   PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY

Binding and elution behavior of proteins on strong cation exchangers

(2009) Timothy M. Pabst et al.   JOURNAL OF CHROMATOGRAPHY A

Comparison of agarose and dextran-grafted agarose strong ion exchangers for the separation of protein aggregates

(2009) Eric J. Suda et al.   JOURNAL OF CHROMATOGRAPHY A

Effect of PEG molecular weight and linking chemistry on the biological activity and thermal stability of PEGylated trypsin

(2008) Benjaporn Treetharnmathurot et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

Effects of ionic strength and mobile phase pH on the binding orientation of lysozyme on different ion-exchange adsorbents

(2008) Florian Dismer et al.   JOURNAL OF CHROMATOGRAPHY A

Separation of PEGylated from unmodified ribonuclease A using sepharose media

(2008) Mayra Cisneros-Ruiz et al.   SEPARATION AND PURIFICATION TECHNOLOGY

Solution Structure of Poly(ethylene) Glycol-Conjugated Hemoglobin Revealed by Small-Angle X-Ray Scattering: Implications for a New Oxygen Therapeutic

(2007) Dmitri I. Svergun et al.   BIOPHYSICAL JOURNAL