Antigen generation and display in therapeutic antibody drug discovery - a neglected but critical player

Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications

(2012) Carissa L. Young et al.   Biotechnology Journal

Deep sequencing analysis of phage libraries using Illumina platform

(2012) Wadim L. Matochko et al.   METHODS

Phage display screening without repetitious selection rounds

(2011) Peter A.C. ’t Hoen et al.   ANALYTICAL BIOCHEMISTRY

Refolding of proteins from inclusion bodies: rational design and recipes

(2011) Anindya Basu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Insect cells as factories for biomanufacturing

(2011) Jean-Christophe Drugmand et al.   BIOTECHNOLOGY ADVANCES

Utilization of non-AUG initiation codons in a flow cytometric method for efficient selection of recombinant cell lines

(2011) Victor R. Cairns et al.   BIOTECHNOLOGY AND BIOENGINEERING

Tuning different expression parameters to achieve soluble recombinant proteins in E. coli: Advantages of high-throughput screening

(2011) Agustín Correa et al.   Biotechnology Journal

Seeing the Light: Luminescent Reporter Gene Assays

(2011) Loren J. Miraglia et al.   COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING

Introduction to current and future protein therapeutics: A protein engineering perspective

(2011) Paul J. Carter   EXPERIMENTAL CELL RESEARCH

A new glycoengineered insect cell line with an inducibly mammalianized protein N-glycosylation pathway

(2011) Jared J Aumiller et al.   GLYCOBIOLOGY

Efficient Large Volume Lentiviral Vector Production Using Flow Electroporation

(2011) Scott R. Witting et al.   HUMAN GENE THERAPY

A simple high-yielding process for transient gene expression in CHO cells

(2011) Yashas Rajendra et al.   JOURNAL OF BIOTECHNOLOGY

Opportunities and challenges for the baculovirus expression system

(2011) Monique M. van Oers   JOURNAL OF INVERTEBRATE PATHOLOGY

Thermostabilisation of an Agonist-Bound Conformation of the Human Adenosine A2A Receptor

(2011) Guillaume Lebon et al.   JOURNAL OF MOLECULAR BIOLOGY

A simplified polyethylenimine-mediated transfection process for large-scale and high-throughput applications

(2011) Céline Raymond et al.   METHODS

Optimizing the baculovirus expression vector system

(2011) Richard B. Hitchman et al.   METHODS

Production and characterization of peptide antibodies

(2011) N.H. Trier et al.   METHODS

Strain engineering for improved expression of recombinant proteins in bacteria

(2011) Tomohiro Makino et al.   Microbial Cell Factories

Diversity of Phage-Displayed Libraries of Peptides during Panning and Amplification

(2011) Ratmir Derda et al.   MOLECULES

Design of Experiment in CHO and HEK transient transfection condition optimization

(2011) Frederic Bollin et al.   PROTEIN EXPRESSION AND PURIFICATION

Production of disulfide-bonded proteins in Escherichia coli

(2011) Mehmet Berkmen   PROTEIN EXPRESSION AND PURIFICATION

GFP-based evaluation system of recombinant expression through the secretory pathway in insect cells and its application to the extracellular domains of class C GPCRs

(2011) Yuji Ashikawa et al.   PROTEIN SCIENCE

MultiBac: expanding the research toolbox for multiprotein complexes

(2011) Christoph Bieniossek et al.   TRENDS IN BIOCHEMICAL SCIENCES

Identification of the shortest Aβ-peptide generating highly specific antibodies against the C-terminal end of amyloid-β42

(2011) Trine Veje Axelsen et al.   VACCINE

Quality requirements for critical assay reagents used in bioanalysis of therapeutic proteins: what bioanalysts should know about their reagents

(2011) Roland F Staack et al.   Bioanalysis

Bioanalytical approaches to analyzing peptides and proteins by LC–MS/MS

(2011) Matthew Ewles et al.   Bioanalysis

Cell-based assays: fuelling drug discovery

(2010) Elisa Michelini et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Disposable bioreactors: the current state-of-the-art and recommended applications in biotechnology

(2010) Regine Eibl et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Selection of soluble protein expression constructs: the experimental determination of protein domain boundaries

(2010) Michael R. Dyson   BIOCHEMICAL SOCIETY TRANSACTIONS

Technical refolding of proteins: Do we have freedom to operate?

(2010) Maria K. Eiberle et al.   Biotechnology Journal

Use of the GTPγS ([35S]GTPγS and Eu-GTPγS) binding assay for analysis of ligand potency and efficacy at G protein-coupled receptors

(2010) Philip G. Strange   BRITISH JOURNAL OF PHARMACOLOGY

Label-free screening assays: a strategy for finding better drug candidates

(2010) Charles A Lunn   Future Medicinal Chemistry

The state of antibody therapy

(2010) Ali ElBakri et al.   HUMAN IMMUNOLOGY

New baculovirus expression tools for recombinant protein complex production

(2010) Simon Trowitzsch et al.   JOURNAL OF STRUCTURAL BIOLOGY

CXCR4 nanobodies (VHH-based single variable domains) potently inhibit chemotaxis and HIV-1 replication and mobilize stem cells

(2010) S. Jahnichen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Computational analysis of off-rate selection experiments to optimize affinity maturation by directed evolution

(2010) Christian Zahnd et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Bridging the gap: A GFP-based strategy for overexpression and purification of membrane proteins with intra and extracellular C-termini

(2010) Jennifer M. Hsieh et al.   PROTEIN SCIENCE

Strategies for analysing and improving the expression and quality of recombinant proteins made in mammalian cells

(2009) Nigel Jenkins et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Next generation immunotherapeutics—honing the magic bullet

(2009) Carrie Enever et al.   CURRENT OPINION IN BIOTECHNOLOGY

The ABC of clinical and experimental adjuvants—A brief overview

(2009) Richard Brunner et al.   IMMUNOLOGY LETTERS

Characterization of High-Molecular-Weight Nonnative Aggregates and Aggregation Kinetics by Size Exclusion Chromatography With Inline Multi-Angle Laser Light Scattering

(2009) Yi Li et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Virus-like Particles as Quantitative Probes of Membrane Protein Interactions†

(2008) Sharon Willis et al.   BIOCHEMISTRY

Optimal conditions for freezing CHO-S and HEK293-EBNA cell lines: Influence of Me2SO, freeze density, and PEI-mediated transfection on revitalization and growth of cells, and expression of recombinant protein

(2008) Marika I. Kleman et al.   BIOTECHNOLOGY AND BIOENGINEERING

Large-scale production of functional membrane proteins

(2008) F. Junge et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Vaccine adjuvant systems: Enhancing the efficacy of sub-unit protein antigens

(2008) Yvonne Perrie et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae

(2008) David Drew et al.   Nature Protocols

Cell-free protein synthesis: applications in proteomics and biotechnology

(2008) Mingyue He   New Biotechnology

A small-scale method for the preparation of plant N-linked glycans from soluble proteins for analysis by MALDI-TOF mass spectrometry

(2008) Saskia R. Karg et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

Reflections on more than 10 years of TGE approaches

(2008) Sabine Geisse   PROTEIN EXPRESSION AND PURIFICATION