Tuning different expression parameters to achieve soluble recombinant proteins in E. coli: Advantages of high-throughput screening

Tuned Escherichia coli as a host for the expression of disulfide-rich proteins

(2011) Gustavo Salinas et al.   Biotechnology Journal

Marker-free plasmids for gene therapeutic applications—Lack of antibiotic resistance gene substantially improves the manufacturing process

(2010) Jürgen Mairhofer et al.   JOURNAL OF BIOTECHNOLOGY

The high-throughput protein sample production platform of the Northeast Structural Genomics Consortium

(2010) Rong Xiao et al.   JOURNAL OF STRUCTURAL BIOLOGY

Applications of the Restriction Free (RF) cloning procedure for molecular manipulations and protein expression

(2010) Tamar Unger et al.   JOURNAL OF STRUCTURAL BIOLOGY

Side effects of chaperone gene co-expression in recombinant protein production

(2010) Mónica Martínez-Alonso et al.   Microbial Cell Factories

Towards universal systems for recombinant gene expression

(2010) Hans Sørensen   Microbial Cell Factories

Production of recombinant proteins in E. coli by the heat inducible expression system based on the phage lambda pL and/or pR promoters

(2010) Norma A Valdez-Cruz et al.   Microbial Cell Factories

Screening optimized protein purification protocols by coupling small-scale expression and mini-size exclusion chromatography

(2010) Elisa Sala et al.   PROTEIN EXPRESSION AND PURIFICATION

Production of protein complexes via co-expression

(2010) John J. Kerrigan et al.   PROTEIN EXPRESSION AND PURIFICATION

The pURI family of expression vectors: A versatile set of ligation independent cloning plasmids for producing recombinant His-fusion proteins

(2010) José Antonio Curiel et al.   PROTEIN EXPRESSION AND PURIFICATION

Conformational stability of pGEX-expressed Schistosoma japonicum glutathione S-transferase: A detoxification enzyme and fusion-protein affinity tag

(2010) Warren Kaplan et al.   PROTEIN SCIENCE

Gene optimization mechanisms: A multi-gene study reveals a high success rate of full-length human proteins expressed inEscherichia coli

(2010) Barbara Maertens et al.   PROTEIN SCIENCE

Soluble Expression inEscherichia coliof Active Human Cyclic Nucleotide Phosphodiesterase Isoform 4B2 in Fusion with Maltose-Binding Protein

(2009) Sha ZHU et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Cell lysis methods for high-throughput screening or miniaturized assays

(2009) Zhanglin Lin et al.   Biotechnology Journal

Over-expression of proteins using a modified pBAD24 vector in E. coli expression system

(2009) Sampali Banerjee et al.   BIOTECHNOLOGY LETTERS

Chaperone-fusion expression plasmid vectors for improved solubility of recombinant proteins in Escherichia coli

(2009) Christos A. Kyratsous et al.   GENE

High-level production of a candidacidal peptide lactoferrampin in Escherichia coli by fusion expression

(2009) Yalin Yang et al.   JOURNAL OF BIOTECHNOLOGY

Rare codon content affects the solubility of recombinant proteins in a codon bias-adjusted Escherichia coli strain

(2009) Germán L Rosano et al.   Microbial Cell Factories

Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli

(2009) Ario de Marco   Microbial Cell Factories

Enabling IMAC purification of low abundance recombinant proteins from E. coli lysates

(2009) Audur Magnusdottir et al.   NATURE METHODS

Expression and purification of the calcium binding photoprotein mitrocomin using ZZ-domain as a soluble partner in E. coli cells

(2009) Satoshi Inouye et al.   PROTEIN EXPRESSION AND PURIFICATION

Single-step affinity purification of recombinant proteins using the silica-binding Si-tag as a fusion partner

(2009) Takeshi Ikeda et al.   PROTEIN EXPRESSION AND PURIFICATION

Protein production and purification

(2008) Susanne Gräslund et al.   NATURE METHODS

Tuning Escherichia coli for membrane protein overexpression

(2008) S. Wagner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Facile, reagentless and in situ release of Escherichia coli intracellular enzymes by heat-inducible autolytic vector for high-throughput screening

(2008) Z. Cai et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Maltose binding protein facilitates high-level expression and functional purification of the chemokines RANTES and SDF-1α from Escherichia coli

(2008) Hee-Jeong Cho et al.   PROTEIN EXPRESSION AND PURIFICATION

Soluble expression of archaeal proteins in Escherichia coli by using fusion-partners

(2008) Seonghun Kim et al.   PROTEIN EXPRESSION AND PURIFICATION

Enhanced Bacterial Protein Expression During Auto-Induction Obtained by Alteration of Lac Repressor Dosage and Medium Composition

(2007) Paul G. Blommel et al.   BIOTECHNOLOGY PROGRESS

Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts

(2007) Heath E. Klock et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Production of Soluble Human Interleukin-6 in Cytoplasm by Fed-Batch Culture of Recombinant E. coli

(2005) Tae Wan Kim et al.   BIOTECHNOLOGY PROGRESS

High-throughput automated refolding screening of inclusion bodies

(2004) Renaud Vincentelli et al.   PROTEIN SCIENCE

Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli

(2002) Martin Hammarström et al.   PROTEIN SCIENCE