Article
Medicine, General & Internal
Juan Carlos Cordova, Sheng Sun, Jeffrey Bos, Srinath Thirumalairajan, Sanjeevani Ghone, Miyako Hirai, Ricarda A. Busse, Julia S. v. der Hardt, Ian Schwartz, Jieyu Zhou
Summary: Membrane chromatography, specifically utilizing Sartobind(R) S and Phenyl membranes, is used to remove aggregates and excess payload in ADC downstream processing. This approach effectively improves the purity of ADCs by clearing unwanted species and increasing homogeneity of drug-to-antibody ratio (DAR).
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Evolene Desligniere, Helene Diemer, Stephane Erb, Pierre Coliat, Xavier Pivot, Alexandre Detappe, Oscar Hernandez-Alba, Sarah Cianferani
Summary: This study provides an overview of the chromatographic tools available for the characterization of monoclonal antibody formats, exemplified by the antibody-drug conjugate trastuzumab deruxtecan. By combining different chromatographic techniques with native mass spectrometry, a comprehensive characterization of the antibody-drug conjugate can be achieved without extensive sample preparation.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2022)
Review
Engineering, Chemical
Xing Yang, Andrea Merenda, Riyadh AL-Attabi, Ludovic F. Dumee, Xiwang Zhang, San H. Thang, Hung Pham, Lingxue Kong
Summary: Membrane chromatography is considered a potential solution for downstream processing in protein purification. Despite limited commercial adoption in large-scale production, significant progress has been made. However, robust evaluation of its performance is still needed, and the disconnection between academic and commercial efforts should be addressed.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Biochemical Research Methods
Zhen Liu, Zheng Fang, Keyun Wang, Mingliang Ye
Summary: A new strategy combining hydrophobic derivatization and high pH strong cation exchange enrichment has been developed to expand the identification coverage of the methylproteome. This strategy successfully identified more methylation sites.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Busra Gurel, Melike Berksoz, Eda Capkin, Ayhan Parlar, Meltem Corbacioglu Pala, Aylin Ozkan, Yilmaz Capan, Duygu Emine Daglikoca, Meral Yuce
Summary: Avastin (R) is a recombinant monoclonal antibody used in cancer treatment. The biosimilar candidate SIMAB054 developed in this study showed different charge variant profile compared to the innovator product. The structural and functional analysis revealed that they share common glycoforms and post-translational modifications, but with different ratios in some samples. The differences mainly originated from the presence of extra C-terminal lysine residues.
Article
Biochemical Research Methods
Liesa Verscheure, An Cerdobbel, Pat Sandra, Frederic Lynen, Koen Sandra
Summary: This study reported a fully automated method for characterizing monoclonal antibody charge variants, which significantly reduces operation time and sample loss, and improves information gathering efficiency. The method can effectively reveal major and minor charge variants, as well as post-translational modifications and modification sites.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Sunil Kumar, Vineela Peruri, Anurag S. Rathore
Summary: In this study, an online volatile-salt-containing two-dimensional liquid chromatography (2D-LC) method coupled with mass spectrometry (MS) was used to characterize the charge heterogeneity of monoclonal antibodies (mAbs). The 2D-LC method successfully separated and identified more mAb variants, including minor variations in charge distribution or conformation that could not be detected by single analytical methods alone.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Biochemical Research Methods
Yifeng Li
Summary: For monoclonal antibodies produced in mammalian cells, ensuring viral safety is crucial. The combination of column chromatography, virus inactivation, and nanofiltration can effectively clear potential endogenous and adventitious viruses.
PROTEIN EXPRESSION AND PURIFICATION
(2022)
Article
Biochemical Research Methods
Amarande Murisier, Bastiaan L. Duivelshof, Szabolcs Fekete, Julien Bourquin, Andrew Schmudlach, Matthew A. Lauber, Jennifer M. Nguyen, Alain Beck, Davy Guillarme, Valentina D'Atri
Summary: This study successfully demonstrated the direct hyphenation of cation exchange chromatography with a compact Time of Flight mass spectrometer for analyzing monoclonal antibodies. By testing various buffers and column dimensions, optimal chromatographic conditions were identified for rapid analysis of charge variants.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Heikki Saari, Reetta Pusa, Heli Marttila, Marjo Yliperttula, Saara Laitinen
Summary: The traditional methods for purifying extracellular vesicles have limitations such as induced aggregation, low yields, poor scalability, and one-dimensional separation process. Ion exchange chromatography, especially anion exchange chromatography, has been evaluated as a promising alternative for extracellular vesicle purification. However, cation exchange chromatography could also be applicable due to cationic domains on the vesicle surface. The choice of resin structure for cation exchange chromatography is critical, as the conventional-type cation exchanger only captures and elutes less than 20% of extracellular vesicles, while the tentacle-type resin can achieve comparable yields and superior purity when combined with conventional cation exchange resin.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Gaoya Yuan, Xudong Zhang, Yuanyi Zhang, Wanyuan Dong, Yifeng Li
Summary: In this work, a method for continuous separation and collection of acidic and basic charge variants was developed, enabling efficient generation of these variants with high purity.
PROTEIN EXPRESSION AND PURIFICATION
(2023)
Article
Chemistry, Analytical
Jelle De Vos, Patricia Pereira Aguilar, Christoph Koeppl, Andreas Fischer, Clemens Gruenwald-Gruber, Mark Duerkop, Miriam Klausberger, Juergen Mairhofer, Gerald Striedner, Monika Cserjan-Puschmann, Alois Jungbauer, Nico Lingg
Summary: The NP protein of SARS-CoV-2 is crucial for viral life cycle and can be a potential diagnostic target, with high titers being expressed in E. coli. Purification methods can yield high purity NP products, and the HIC-MALS method allows for monitoring of product purity and molecular mass.
Review
Chemistry, Applied
Solmaz Hajizadeh
Summary: The demand for efficient separation and purification methods has led to the development of new techniques and materials, such as composite cryogels. These composite cryogels can directly purify targets from particulate-containing fluids and offer higher binding capacities, improved selectivity, and enhanced overall performance compared to conventional chromatographic columns.
REACTIVE & FUNCTIONAL POLYMERS
(2023)
Article
Engineering, Chemical
Elena Lietta, Alessandro Pieri, Antonio G. Cardillo, Marco Vanni, Roberto Pisano, Antonello A. Barresi
Summary: This work investigates the application of Hydrophobic Interaction Chromatography (HIC) as a polishing step in the purification of therapeutic proteins. Two different approaches for studying HIC are compared, with the predictive approach providing more information but requiring a higher experimental effort, and the estimation approach being more effective but resulting in less knowledge gained. One of the approaches is also successfully applied to an industrial purification process.
Article
Chemistry, Multidisciplinary
Oliver Musl, Irina Sulaeva, Ivan Sumerskii, Arnulf Kai Mahler, Thomas Rosenau, Jana Falkenhagen, Antje Potthast
Summary: Lignosulfonates are industrial biorefinery products with significant variability and heterogeneity in their structural composition. This study utilizes a novel hydrophobic chromatography method to provide a comprehensive understanding of the characteristic composition of lignosulfonates and reveals significant differences in hydrophobic composition among different industrial lignosulfonates.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)