Towards a simple on-line coupling of ion exchange chromatography and native mass spectrometry for the detailed characterization of monoclonal antibodies

Comparative Elucidation of Cetuximab Heterogeneity on the Intact Protein Level by Cation Exchange Chromatography and Capillary Electrophoresis Coupled to Mass Spectrometry

(2020) Florian Fussl et al.   ANALYTICAL CHEMISTRY

Impact of the column on effluent pH in cation exchange pH gradient chromatography, a practical study

(2020) Evelin Farsang et al.   JOURNAL OF CHROMATOGRAPHY A

Coupling non-denaturing chromatography to mass spectrometry for the characterization of monoclonal antibodies and related products

(2020) Evelin Farsang et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Recent advances in LC–MS based characterization of protein-based bio-therapeutics – mastering analytical challenges posed by the increasing format complexity

(2020) Tobias Graf et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Application of Native Ion Exchange Mass Spectrometry to Intact and Subunit Analysis of Site-Specific Antibody–Drug Conjugates Produced by AJICAP First Generation Technology

(2020) Yutaka Matsuda et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Versatile, Sensitive, and Robust Native LC–MS Platform for Intact Mass Analysis of Protein Drugs

(2020) Yuetian Yan et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Optimisation of the use of sliding window deconvolution for comprehensive characterisation of trastuzumab and adalimumab charge variants by native high resolution mass spectrometry

(2020) Silvia Millán-Martín et al.   EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

A generic workflow for the characterization of therapeutic monoclonal antibodies—application to daratumumab

(2019) Bastiaan L. Duivelshof et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Tuning selectivity in cation-exchange chromatography applied for monoclonal antibody separations, part 1: Alternative mobile phases and fine tuning of the separation

(2019) Evelin Farsang et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Tuning selectivity in cation-exchange chromatography applied for monoclonal antibody separations, part 2: Evaluation of recent stationary phases

(2019) Amarande Murisier et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Charge Variant Analysis of Monoclonal Antibodies Using Direct Coupled pH Gradient Cation Exchange Chromatography to High-Resolution Native Mass Spectrometry

(2018) Florian Füssl et al.   ANALYTICAL CHEMISTRY

Rapid charge variant analysis of monoclonal antibodies to support lead candidate biopharmaceutical development

(2018) Anne Trappe et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Ultrasensitive Characterization of Charge Heterogeneity of Therapeutic Monoclonal Antibodies Using Strong Cation Exchange Chromatography Coupled to Native Mass Spectrometry

(2018) Yuetian Yan et al.   ANALYTICAL CHEMISTRY

How Similar Is Biosimilar? A Comparison of Infliximab Therapeutics in Regard to Charge Variant Profile and Antigen Binding Affinity

(2018) Beate Beyer et al.   Biotechnology Journal

Identification and quantification of product-related quality attributes in bio-therapeutic monoclonal antibody via a simple, and robust cation-exchange HPLC method compatible with direct online detection of UV and native ESI-QTOF-MS analysis

(2018) Praveen Kallamvalliillam Sankaran et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Charge variants characterization of a monoclonal antibody by ion exchange chromatography coupled on-line to native mass spectrometry: Case study after a long-term storage at +5 °C

(2017) Y. Leblanc et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Determination of isoelectric points and relative charge variants of 23 therapeutic monoclonal antibodies

(2017) Alexandre Goyon et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Advances in native high-performance liquid chromatography and intact mass spectrometry for the characterization of biopharmaceutical products

(2017) Marco Tassi et al.   JOURNAL OF SEPARATION SCIENCE

Unbiased in-depth characterization of CEX fractions from a stressed monoclonal antibody by mass spectrometry

(2017) François Griaud et al.   mAbs

Emerging Strategies for Developing Next-Generation Protein Therapeutics for Cancer Treatment

(2016) James R. Kintzing et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Characterization of Intact Protein Conjugates and Biopharmaceuticals Using Ion-Exchange Chromatography with Online Detection by Native Electrospray Ionization Mass Spectrometry and Top-Down Tandem Mass Spectrometry

(2015) Khaja Muneeruddin et al.   ANALYTICAL CHEMISTRY

Method development for the separation of monoclonal antibody charge variants in cation exchange chromatography, Part I: Salt gradient approach

(2015) Szabolcs Fekete et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Method development for the separation of monoclonal antibody charge variants in cation exchange chromatography, Part II: pH gradient approach

(2015) Szabolcs Fekete et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Effects of Ammonium Bicarbonate on the Electrospray Mass Spectra of Proteins: Evidence for Bubble-Induced Unfolding

(2013) Jason B. Hedges et al.   ANALYTICAL CHEMISTRY

Charge heterogeneity profiling of monoclonal antibodies using low ionic strength ion-exchange chromatography and well-controlled pH gradients on monolithic columns

(2013) Mohammad Talebi et al.   JOURNAL OF CHROMATOGRAPHY A

Chromatographic analysis of the acidic and basic species of recombinant monoclonal antibodies

(2012) Yi Du et al.   mAbs

Simultaneous Assessment of Asp Isomerization and Asn Deamidation in Recombinant Antibodies by LC-MS following Incubation at Elevated Temperatures

(2012) Katharina Diepold et al.   PLoS One

On-line characterization of monoclonal antibody variants by liquid chromatography–mass spectrometry operating in a two-dimensional format

(2011) Melissa Alvarez et al.   ANALYTICAL BIOCHEMISTRY

Charge variants in IgG1

(2010) Leslie A. Khawli et al.   mAbs