Challenges and advances in the heterologous expression of cellulolytic enzymes: a review

Engineered thermostable fungal cellulases exhibit efficient synergistic cellulose hydrolysis at elevated temperatures

(2014) Devin L. Trudeau et al.   BIOTECHNOLOGY AND BIOENGINEERING

Protein engineering of cellulases

(2014) Andreas S Bommarius et al.   CURRENT OPINION IN BIOTECHNOLOGY

Protein folding and secretion: mechanistic insights advancing recombinant protein production in S. cerevisiae

(2014) Carissa L Young et al.   CURRENT OPINION IN BIOTECHNOLOGY

Expression of threeTrichoderma reeseicellulase genes inSaccharomyces pastorianusfor the development of a two-step process of hydrolysis and fermentation of cellulose

(2014) J. Fitzpatrick et al.   JOURNAL OF APPLIED MICROBIOLOGY

Expression of endoglucanases in Pichia pastoris under control of the GAP promoter

(2014) Anikó Várnai et al.   Microbial Cell Factories

Recombinant Cellulase Accumulation in the Leaves of Mature, Vegetatively Propagated Transgenic Sugarcane

(2014) Mark D. Harrison et al.   MOLECULAR BIOTECHNOLOGY

A Highly Tunable System for the Simultaneous Expression of Multiple Enzymes in Saccharomyces cerevisiae

(2014) Yoichiro Ito et al.   ACS Synthetic Biology

Metabolic engineering of yeasts by heterologous enzyme production for degradation of cellulose and hemicellulose from biomass: a perspective

(2014) William Kricka et al.   Frontiers in Microbiology

Characterization and High Level Expression of Acidic Endoglucanase in Pichia pastoris

(2013) Ali Akbarzadeh et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Engineering pyruvate decarboxylase-mediated ethanol production in the thermophilic host Geobacillus thermoglucosidasius

(2013) L. J. Van Zyl et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Endowing non-cellulolytic microorganisms with cellulolytic activity aiming for consolidated bioprocessing

(2013) Ryosuke Yamada et al.   BIOTECHNOLOGY ADVANCES

Ethanol inducible expression of a mesophilic cellulase avoids adverse effects on plant development

(2013) Holger Klose et al.   Biotechnology for Biofuels

Assembling a cellulase cocktail and a cellodextrin transporter into a yeast host for CBP ethanol production

(2013) Jui-Jen Chang et al.   Biotechnology for Biofuels

Secretory pathway of cellulase: a mini-review

(2013) Shaomin Yan et al.   Biotechnology for Biofuels

Improving activity of minicellulosomes by integration of intra- and intermolecular synergies

(2013) Qi Xu et al.   Biotechnology for Biofuels

Expression of cellulase genes in Saccharomyces cerevisiae via δ-integration subject to auxotrophic markers

(2013) Lin Liu et al.   BIOTECHNOLOGY LETTERS

Genetic engineering of the chloroplast: novel tools and new applications

(2013) Ralph Bock   CURRENT OPINION IN BIOTECHNOLOGY

Improved production of secreted heterologous enzyme in Bacillus subtilis strain MGB874 via modification of glutamate metabolism and growth conditions

(2013) Kenji Manabe et al.   Microbial Cell Factories

Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidase

(2013) Sujin Kim et al.   Microbial Cell Factories

Enhanced production of carboxymethylcellulase of a marine microorganism, Bacillus subtilis subsp. subtilis A-53 in a pilot-scaled bioreactor by a recombinant Escherichia coli JM109/A-53 from rice bran

(2013) Eun-Jung Lee et al.   MOLECULAR BIOLOGY REPORTS

Improved recombinant cellulase expression in chloroplast of tobacco through promoter engineering and 5′ amplification promoting sequence

(2013) Sera Jung et al.   PLANT MOLECULAR BIOLOGY

Glycosylated linkers in multimodular lignocellulose-degrading enzymes dynamically bind to cellulose

(2013) Christina M. Payne et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cellulases for biomass degradation: comparing recombinant cellulase expression platforms

(2013) Megan Garvey et al.   TRENDS IN BIOTECHNOLOGY

A Genome-Wide Activity Assessment of Terminator Regions in Saccharomyces cerevisiae Provides a ″Terminatome″ Toolbox

(2013) Mamoru Yamanishi et al.   ACS Synthetic Biology

Increasing protein production by directed vector backbone evolution

(2013) Felix Jakob et al.   AMB Express

Efficient direct ethanol production from cellulose by cellulase- and cellodextrin transporter-co-expressing Saccharomyces cerevisiae

(2013) Ryosuke Yamada et al.   AMB Express

Engineering microbial surfaces to degrade lignocellulosic biomass

(2013) Grace L Huang et al.   Bioengineered

Flow cytometry-based ultra-high-throughput screening assay for cellulase activity

(2012) Raluca Ostafe et al.   ANALYTICAL BIOCHEMISTRY

Specific Fusion of β-1,4-Endoglucanase and β-1,4-Glucosidase Enhances Cellulolytic Activity and Helps in Channeling of Intermediates

(2012) Nidhi Adlakha et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Recombinant Bacillus subtilis That Grows on Untreated Plant Biomass

(2012) Timothy D. Anderson et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Overexpression of native PSE1 and SOD1 in Saccharomyces cerevisiae improved heterologous cellulase secretion

(2012) Heinrich Kroukamp et al.   APPLIED ENERGY

A review of lignocellulose bioconversion using enzymatic hydrolysis and synergistic cooperation between enzymes—Factors affecting enzymes, conversion and synergy

(2012) J.S. Van Dyk et al.   BIOTECHNOLOGY ADVANCES

Paradigmatic status of an endo- and exoglucanase and its effect on crystalline cellulose degradation

(2012) Sarah Moraïs et al.   Biotechnology for Biofuels

PGASO: A synthetic biology tool for engineering a cellulolytic yeast

(2012) Jui-Jen Chang et al.   Biotechnology for Biofuels

Hyperthermophilic endoglucanase for in planta lignocellulose conversion

(2012) Holger Klose et al.   Biotechnology for Biofuels

Promoter engineering: Recent advances in controlling transcription at the most fundamental level

(2012) John Blazeck et al.   Biotechnology Journal

Present and potential applications of cellulases in agriculture, biotechnology, and bioenergy

(2012) Paripok Phitsuwan et al.   FOLIA MICROBIOLOGICA

Cellulose Degradation by Sulfolobus solfataricus Requires a Cell-Anchored Endo-β-1-4-Glucanase

(2012) Michele Girfoglio et al.   JOURNAL OF BACTERIOLOGY

Effective approach to greatly enhancing selective secretion and expression of three cytoplasmic enzymes in Escherichia coli through synergistic effect of EDTA and lysozyme

(2012) Sen-Lin Liu et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Engineering a thermoregulated intein-modified xylanase into maize for consolidated lignocellulosic biomass processing

(2012) Binzhang Shen et al.   NATURE BIOTECHNOLOGY

Modification of esterified cell wall phenolics increases vulnerability of tall fescue to herbivory by the fall armyworm

(2012) Marcia M. de O. Buanafina et al.   PLANTA

Cellobiohydrolase secretion by yeast: Current state and prospects for improvement

(2012) Riaan den Haan et al.   PROCESS BIOCHEMISTRY

Cultivation at 6–10°C is an effective strategy to overcome the insolubility of recombinant proteins in Escherichia coli

(2012) Jung Min Song et al.   PROTEIN EXPRESSION AND PURIFICATION

Expression of an Acidothermus cellulolyticus endoglucanase in transgenic rice seeds

(2012) Qing Zhang et al.   PROTEIN EXPRESSION AND PURIFICATION

Enhanced expression of an endoglucanase in Bacillus subtilis by using the sucrose-inducible sacB promoter and improved properties of the recombinant enzyme

(2012) Sen-Lin Liu et al.   PROTEIN EXPRESSION AND PURIFICATION

Efficient extracellular secretion of an endoglucanase and a β-glucosidase in E. coli

(2012) Shefali Gupta et al.   PROTEIN EXPRESSION AND PURIFICATION

A nanocluster design for the construction of artificial cellulosomes

(2012) Do-Myoung Kim et al.   Catalysis Science & Technology

Synthesis and Characterization of Chimeric Proteins Based on Cellulase and Xylanase from an Insect Gut Bacterium

(2011) Nidhi Adlakha et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Assembly of Minicellulosomes on the Surface of Bacillus subtilis

(2011) Timothy D. Anderson et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The Issue of Secretion in Heterologous Expression of Clostridium cellulolyticum Cellulase-Encoding Genes in Clostridium acetobutylicum ATCC 824

(2011) Florence Mingardon et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Cloning of Thermostable Cellulase Genes of Clostridium thermocellum and Their Secretive Expression in Bacillus subtilis

(2011) Jian-Ming Liu et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Co-expression of a cellobiose phosphorylase and lactose permease enables intracellular cellobiose utilisation by Saccharomyces cerevisiae

(2011) Christa J. Sadie et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Direct ethanol production from cassava pulp using a surface-engineered yeast strain co-displaying two amylases, two cellulases, and β-glucosidase

(2011) Waraporn Apiwatanapiwat et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Consolidated Pretreatment and Hydrolysis of Plant Biomass Expressing Cell Wall Degrading Enzymes

(2011) Dongcheng Zhang et al.   BioEnergy Research

Industrial yeast strain engineered to ferment ethanol from lignocellulosic biomass

(2011) Nikolai Khramtsov et al.   BIORESOURCE TECHNOLOGY

High level secretion of cellobiohydrolases by Saccharomyces cerevisiae

(2011) Marja Ilmén et al.   Biotechnology for Biofuels

In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize

(2011) Roman Brunecky et al.   Biotechnology for Biofuels

How recombinant swollenin from Kluyveromyces lactis affects cellulosic substrates and accelerates their hydrolysis

(2011) Gernot Jäger et al.   Biotechnology for Biofuels

Trichoderma reesei cellobiohydrolase II is associated with the outer membrane when overexpressed in Escherichia coli

(2011) Diya M. Abdeljabbar et al.   BIOTECHNOLOGY LETTERS

Expression of a secretory β-glucosidase from Trichoderma reesei in Pichia pastoris and its characterization

(2011) Ping Chen et al.   BIOTECHNOLOGY LETTERS

Effect of codon optimization on the expression of Trichoderma reesei endoglucanase 1 in Pichia pastoris

(2011) Gunseli Bayram Akcapinar et al.   BIOTECHNOLOGY PROGRESS

Recent progress in consolidated bioprocessing

(2011) Daniel G Olson et al.   CURRENT OPINION IN BIOTECHNOLOGY

Production of minicellulosomes for the enhanced hydrolysis of cellulosic substrates by recombinant Corynebacterium glutamicum

(2011) Jeong Eun Hyeon et al.   ENZYME AND MICROBIAL TECHNOLOGY

Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharification processes

(2011) Mathieu Bey et al.   Microbial Cell Factories

Trichoderma reesei RUT-C30 - thirty years of strain improvement

(2011) R. Peterson et al.   MICROBIOLOGY-SGM

Accumulation of recombinant cellobiohydrolase and endoglucanase in the leaves of mature transgenic sugar cane

(2011) Mark D. Harrison et al.   PLANT BIOTECHNOLOGY JOURNAL

Manipulating corn germplasm to increase recombinant protein accumulation

(2011) Elizabeth E. Hood et al.   PLANT BIOTECHNOLOGY JOURNAL

High-level expression of a suite of thermostable cell wall-degrading enzymes from the chloroplast genome

(2011) Kerstin Petersen et al.   PLANT MOLECULAR BIOLOGY

An efficient downstream box fusion allows high-level accumulation of active bacterial beta-glucosidase in tobacco chloroplasts

(2011) Benjamin N. Gray et al.   PLANT MOLECULAR BIOLOGY

Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli

(2011) G. Bokinsky et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enhancement of Cellulolytic Enzyme Activity by Clustering Cellulose Binding Domains on Nanoscaffolds

(2011) Do-Myoung Kim et al.   Small

Alternatives to Trichoderma reesei in biofuel production

(2011) Alexander V. Gusakov   TRENDS IN BIOTECHNOLOGY

Engineering new metabolic capabilities in bacteria: lessons from recombinant cellulolytic strategies

(2011) Roberto Mazzoli et al.   TRENDS IN BIOTECHNOLOGY

Deconstruction of Lignocellulosic Biomass to Fuels and Chemicals

(2011) Shishir P.S. Chundawat et al.   Annual Review of Chemical and Biomolecular Engineering

Cellulosomes: Highly Efficient Nanomachines Designed to Deconstruct Plant Cell Wall Complex Carbohydrates

(2010) Carlos M.G.A. Fontes et al.   Annual Review of Biochemistry

The Genetics of Lignin Biosynthesis: Connecting Genotype to Phenotype

(2010) Nicholas D. Bonawitz et al.   Annual Review of Genetics

Surface Display of a Functional Minicellulosome by Intracellular Complementation Using a Synthetic Yeast Consortium and Its Application to Cellulose Hydrolysis and Ethanol Production

(2010) S.-L. Tsai et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineering cellulolytic ability into bioprocessing organisms

(2010) Daniel C. la Grange et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Synthetic enzyme mixtures for biomass deconstruction: Production and optimization of a core set

(2010) Goutami Banerjee et al.   BIOTECHNOLOGY AND BIOENGINEERING

Mining metagenomes for novel cellulase genes

(2010) Cheng-Jie Duan et al.   BIOTECHNOLOGY LETTERS

Engineered microbial systems for enhanced conversion of lignocellulosic biomass

(2010) James G Elkins et al.   CURRENT OPINION IN BIOTECHNOLOGY

Production of minicellulosomes from Clostridium cellulovorans for the fermentation of cellulosic ethanol using engineered recombinant Saccharomyces cerevisiae

(2010) Jeong-eun Hyeon et al.   FEMS MICROBIOLOGY LETTERS

Enhanced cellulose degradation by nano-complexed enzymes: Synergism between a scaffold-linked exoglucanase and a free endoglucanase

(2010) Sarah Moraïs et al.   JOURNAL OF BIOTECHNOLOGY

Development of a stationary phase-specific autoinducible expression system in Bacillus subtilis

(2010) Su-Jin Lee et al.   JOURNAL OF BIOTECHNOLOGY

Engineering the cell surface display of cohesins for assembly of cellulosome-inspired enzyme complexes on Lactococcus lactis

(2010) Andrew S Wieczorek et al.   Microbial Cell Factories

Cocktail δ-integration: a novel method to construct cellulolytic enzyme expression ratio-optimized yeast strains

(2010) Ryosuke Yamada et al.   Microbial Cell Factories

Characterization and varied expression of a membrane-bound endo-β-1,4-glucanase in hybrid poplar

(2010) Victoria J. Maloney et al.   PLANT BIOTECHNOLOGY JOURNAL

Autoluminescent Plants

(2010) Alexander Krichevsky et al.   PLoS One

In planta differential targeting analysis of Thermotoga maritima Cel5A and CBM6-engineered Cel5A for autohydrolysis

(2010) Shobana Arumugam Mahadevan et al.   TRANSGENIC RESEARCH

Cellulolytic Systems in Insects

(2009) Hirofumi Watanabe et al.   Annual Review of Entomology

Regulation of the Display Ratio of Enzymes on the Saccharomyces cerevisiae Cell Surface by the Immunoglobulin G and Cellulosomal Enzyme Binding Domains

(2009) J. Ito et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Functional Assembly of Minicellulosomes on the Saccharomyces cerevisiae Cell Surface for Cellulose Hydrolysis and Ethanol Production

(2009) S.-L. Tsai et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Effect of Linker Length and Dockerin Position on Conversion of a Thermobifida fusca Endoglucanase to the Cellulosomal Mode

(2009) J. Caspi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Exploring improved endoglucanase expression in Saccharomyces cerevisiae strains

(2009) Lisa du Plessis et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The noncellulosomal family 48 cellobiohydrolase from Clostridium phytofermentans ISDg: heterologous expression, characterization, and processivity

(2009) Xiao-Zhou Zhang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Cellulases and biofuels

(2009) David B Wilson   CURRENT OPINION IN BIOTECHNOLOGY

Commercial cellulosic ethanol: The role of plant-expressed enzymes

(2009) Manuel B. Sainz   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Cloning of a gene encoding thermostable cellobiohydrolase from the thermophilic fungusChaetomium thermophilumand its expression inPichia pastoris

(2009) Y.-L. Li et al.   JOURNAL OF APPLIED MICROBIOLOGY

Disruption of the OCH1 and MNN1 genes decrease N-glycosylation on glycoprotein expressed in Kluyveromyces lactis

(2009) Bo Liu et al.   JOURNAL OF BIOTECHNOLOGY

The rosettazyme: A synthetic cellulosome

(2009) Shigenobu Mitsuzawa et al.   JOURNAL OF BIOTECHNOLOGY

Green tissue-specific production of a microbial endo-cellulase in maize (Zea maysL.) endoplasmic-reticulum and mitochondria converts cellulose into fermentable sugars

(2009) Chuansheng Mei et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Loosening Xyloglucan Accelerates the Enzymatic Degradation of Cellulose in Wood

(2009) Rumi Kaida et al.   Molecular Plant

Expression ofAcidothermus cellulolyticusE1 endo-β-1,4-glucanase catalytic domain in transplastomic tobacco

(2009) Thomas Ziegelhoffer et al.   PLANT BIOTECHNOLOGY JOURNAL

A family of thermostable fungal cellulases created by structure-guided recombination

(2009) P. Heinzelman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Modulation of cellulosome composition inClostridium cellulolyticum: Adaptation to the polysaccharide environment revealed by proteomic and carbohydrate-active enzyme analyses

(2009) Jean-Charles Blouzard et al.   PROTEOMICS

Secretion and subcellular localizations of bacterial proteins: a semantic awareness issue

(2009) Mickaël Desvaux et al.   TRENDS IN MICROBIOLOGY

Extracellular recombinant protein production from anEscherichia coli lppdeletion mutant

(2008) Hyun-Dong Shin et al.   BIOTECHNOLOGY AND BIOENGINEERING

High-level bacterial cellulase accumulation in chloroplast-transformed tobacco mediated by downstream box fusions

(2008) Benjamin N. Gray et al.   BIOTECHNOLOGY AND BIOENGINEERING

Implications of cellobiohydrolase glycosylation for use in biomass conversion

(2008) Tina Jeoh et al.   Biotechnology for Biofuels

Conversion of Thermobifida fusca free exoglucanases into cellulosomal components: Comparative impact on cellulose-degrading activity

(2008) Jonathan Caspi et al.   JOURNAL OF BIOTECHNOLOGY

Exhaustion of the chloroplast protein synthesis capacity by massive expression of a highly stable protein antibiotic

(2008) Melanie Oey et al.   PLANT JOURNAL

Overexpression of Poplar Cellulase Accelerates Growth and Disturbs the Closing Movements of Leaves in Sengon

(2008) S. Hartati et al.   PLANT PHYSIOLOGY

Heterologous expression of glycosyl hydrolases in planta: a new departure for biofuels

(2008) Larry E. Taylor et al.   TRENDS IN BIOTECHNOLOGY