Article
Chemistry, Multidisciplinary
Juntao Zhao, Feng Li, Shutian Kong, Tao Chen, Hao Song, Zhiwen Wang
Summary: By synthesizing riboflavin and overexpressing the outer membrane cytochrome MtrC and the cell division inhibitor sulA, Shewanella oneidensis achieves enhanced extracellular electron transfer (EET). Moreover, a hybrid biofilm composed of riboflavin, multiwalled carbon nanotubes (MWCNTs), and graphene oxide (GO) significantly increases power generation and reduces charge-transfer resistance.
Article
Microbiology
Xiao Zhu, Fei Dou, Mingliang Long, Xinxin Wang, Wei Liu, Fangbai Li, Tongxu Liu, Yundang Wu
Summary: This study found that AQS can promote both biofilm formation and electron shuttling in S. oneidensis MR-1, but excessive AQS may hinder biofilm formation. The results also demonstrated that microbial-catalyzed ES bio-reduction is the key limiting factor of the maximum biocurrent density in BESs.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ning Xu, Tai-Lin Wang, Wen-Jie Li, Yan Wang, Jie-Jie Chen, Jun Liu
Summary: This study investigated the effects of different electron shuttles on electron transfer in Shewanella oneidensis MR-1 and found that anthraquinone-2-sulfonate (AQS) led to the highest cathodic current density, total charge production, and reduction product formation. Additionally, the introduction of -OH or -NH2 group into AQS significantly affected redox potentials, with AQS-1-NH2 showing lower redox potential and higher Coulombic efficiency compared to AQS. The study also showed that cytochromes MtrA and MtrB are essential for optimal AQS-mediated electron transfer in S. oneidensis MR-1.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Chemical
Xizi Long, Hongrui Zhang, Xian Cao, Hui Wang, Kohei Shimokawa, Huimei Chi, Chengming Zhang, Akihiro Okamoto, Xianning Li
Summary: The research shows that Shewanella oneidensis MR-1 can oxidize manganese Mn(II) to form Mn2O3 nanoparticles, driven by cytochrome c reductase Complex III. Genomic analysis indicates that genes encoding Complex III were upregulated during the oxidation process, associated with the inability of mutants to oxidize.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Environmental Sciences
Mengmeng Tang, Chaoyong Wang, Zaitian Dong, Qianjin Che, Zetang Wang, Yuxuan Zhu
Summary: The biological reduction of Fe (III) in underground environments has significant impacts on the biogeochemical cycle of iron and the migration and transformation of pollutants. Humic substances, especially the electron shuttle anthraquinone-2-sulfonate (AQS), can effectively promote the bio-reduction of Fe (III) and enhance the bioavailability of Fe (III). This study demonstrated that aniline, when mediated by AQS, significantly increased the production of Fe (II) and promoted the metabolism of Shewanella oneidensis MR-1 cells, leading to more efficient degradation of aniline. The findings provide insights into the reduction mechanism of Fe (III) in complex environments and offer a theoretical basis for the biodegradation of aromatic hydrocarbon pollutants.
Article
Biotechnology & Applied Microbiology
Atsumi Hirose, Atsushi Kouzuma, Kazuya Watanabe
Summary: Bioelectrochemical systems (BESs) utilize electrochemical interactions between electrochemically active bacteria (EAB) and electrodes, attracting considerable attention. Research suggests that hydrogen can promote specific bacteria's current generation and cell growth, while the cycling of hydrogen may hinder the quantitative evaluation of organic substrate-dependent current generation in BESs.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Multidisciplinary Sciences
Feng Li, Rui Tang, Baocai Zhang, Chunxiao Qiao, Huan Yu, Qijing Liu, Junqi Zhang, Liang Shi, Hao Song
Summary: Researchers systematically engineered Shewanella oneidensis, an exoelectrogenic model organism, to promote biofilm formation and enhance biofilm conductivity in microbial electrochemical systems. The engineered biofilm achieved a maximum output power density of 3.62 +/- 0.06 W m-2, 39.3 times higher than that of the wild-type strain, setting a new record for the output power density of genetically engineered Shewanella biofilms.
Article
Engineering, Environmental
Yuan Li, Kai Liu, Rongrong Mao, Boya Liu, Lei Cheng, Xianyang Shi
Summary: This study examines the chemotactic behaviors of electroactive bacteria (EAB) Shewanella oneidensis MR-1 towards nitroaromatic compounds (NACs) and reveals the underlying molecular mechanism. The research also shows that external riboflavin enhances the chemotaxis response and bioavailability of NACs.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Alejandra Hernandez-Santana, Joseph M. Suflita, Mark A. Nanny
Summary: This study highlights the role of Shewanella oneidensis and its strains in the corrosion of steel through DET and H2 transfer mechanisms. The experiments demonstrate that S. oneidensis accelerates the corrosion rate of steel through DET, and the loss of DET capability significantly reduces the corrosion rate.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2022)
Article
Engineering, Environmental
Siyu Zhang, Changhao Li, Changdong Ke, Sijia Liu, Qian Yao, Weilin Huang, Zhi Dang, Chuling Guo
Summary: Photosensitized biohybrid system (PBS) allows bacteria to utilize light energy harvested by semiconductors for efficient pollutants transformation, showing great potential in water reclamation. Establishing a biocompatible environment during photocatalytic reactions is crucial for the development of PBS-based treatment technologies. Supplying extracellular polymeric substances (EPS) fractions is found to enhance bacterial viability under adverse conditions, improving the performance of PBS for environmental remediation.
Review
Biochemistry & Molecular Biology
Sota Ikeda, Yuki Takamatsu, Miyu Tsuchiya, Keigo Suga, Yugo Tanaka, Atsushi Kouzuma, Kazuya Watanabe
Summary: The genus Shewanella includes various heterotrophic bacteria, some pathogenic and others essential in the global carbon cycle. The representative strain Shewanella oneidensis MR-1 has versatile respiratory capacities and electrochemical activity, making it a focus of research for applications in electro-biotechnology.
MICROBIAL CELL FACTORIES-BOOK
(2021)
Review
Biochemistry & Molecular Biology
Atsushi Kouzuma
Summary: Electrochemically active bacteria (EAB) interact with electrodes via extracellular electron transfer (EET) pathways, dependent on carbon catabolism and respiration regulation, with biofilm formation being crucial for electric current generation.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemical Research Methods
Yuxuan Zang, Hongyu Zhao, Bo Cao, Beizhen Xie, Yue Yi, Hong Liu
Summary: Water toxicity detection is crucial for ensuring water supply safety. A novel microbial electrochemical sensor (MES) using suspended electrochemically active bacteria (EAB) as the sensing element has been reported, but its sensitivity needs further improvement. In this study, the extracellular electron transfer (EET) of the suspended EAB-based MES was optimized to enhance sensitivity. The optimized parameters for inward EET were a potential of - 0.5 V, a cell density of 1.8 x -10(8) CFU/mL, and an electron acceptor concentration of 15 mM.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Agricultural Engineering
Lang Chen, Yang Wu, Quilting Shen, Xiong Zheng, Yinguang Chen
Summary: This study found that Cu NPs exposure significantly enhanced the Cr(VI) reduction efficiency of Shewanella oneidensis MR-1. Cu NPs can influence the bacterial Cr(VI) reduction ability by enhancing electron migration capacity and regulating the expression of key genes.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Keisuke Tomita, Atsumi Hirose, Yugo Tanaka, Atsushi Kouzuma, Kazuya Watanabe
Summary: Electrochemically active bacteria (EAB) can interact electrically with electrodes, making them useful in bioelectrochemical systems (BESs). Controlling the metabolic activities of EAB is crucial for optimizing BES performance. A recent study found that the EAB Shewanella oneidensis MR-1 uses the Arc system to regulate the expression of catabolic genes in response to electrode potentials, suggesting the development of electrogenetics to control gene expression in EAB.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2023)
Article
Microbiology
Isabel R. Baker, Bridget E. Conley, Jeffrey A. Gralnick, Peter R. Girguis
Summary: Some bacteria and archaea have evolved the ability to use extracellular electron transfer (EET) as a means of energy metabolism. The MtrCAB system, which transfers electrons from metabolic substrates to electron acceptors outside the cell, has been found in a diverse range of bacteria. These findings highlight the broad relevance of EET to different taxa and biogeochemical cycles in our biosphere and provide a foundation for further research on its evolution and role in Earth's redox landscape.
Article
Biotechnology & Applied Microbiology
Maurice Finger, Fabio Sentek, Lukas Hartmann, Ana M. Palacio-Barrera, Ivan Schlembach, Miriam A. Rosenbaum, Jochen Buechs
Summary: In this study, high-throughput online monitoring was used to gain a deep understanding of the cultivation process of the model organism Streptomyces coelicolor A3(2). By measuring the oxygen transfer rate and autofluorescence online, valuable information about nutrient availability and pigment product formation patterns can be obtained. The study identified the filling volume of the cultivation vessel as a factor influencing pigment formation.
ENGINEERING IN LIFE SCIENCES
(2023)
Article
Microbiology
Christian-Marco Klask, Benedikt Jaeger, Isabella Casini, Largus T. Angenent, Bastian Molitor
Summary: The RNF complex plays a crucial role in energy conversion in Clostridium ljungdahlii. Disruption of RNF complex genes leads to loss of autotrophy, but can be restored through plasmid complementation. The rseC gene is important for autotrophic growth, and there is a connection between nitrate reduction and energy metabolism.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biochemical Research Methods
Esteban Marcellin, Largus T. Angenent, Lars K. Nielsen, Bastian Molitor
Summary: This review explores the potential of gas fermentation and synthetic biology to transform food production and achieve sustainability.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Editorial Material
Biotechnology & Applied Microbiology
David P. B. T. B. Strik, Ramon Ganigue, Largus T. Angenent
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Environmental
Rodolfo Palomo-Briones, Jiajie Xu, Catherine M. Spirito, Joseph G. Usack, Lauren H. Trondsen, Juan J. L. Guzman, Largus T. Angenent
Summary: pH is a critical parameter in chain-elongating bioreactors, affecting product concentration, thermodynamics, and kinetics. The effect of different pH levels on n-caprylate production was examined, and it was found that mildly acidic pH led to n-caproate production while near-neutral and neutral pH favored n-caprylate production. High concentrations of undissociated n-caprylic acid inhibited methanogenesis at near-neutral and neutral pH. The microbial communities at near-neutral and neutral pH were more diverse.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Agricultural Engineering
Sarah Schulz, Bastian Molitor, Largus T. Angenent
Summary: Recycling waste gases from industry is crucial for transitioning towards a circular economy. A gas fermentation experiment using C. ljungdahlii and pure CO as feedstock was conducted, omitting yeast extract. The results showed that using only CO as the feedstock increased ethanol production rate and selectivity when supplemented with sodium acetate. Oxygen contaminations, however, reversed this effect. These findings emphasize the potential of CO-fermentation with acetate augmentation and the importance of preventing oxygen contaminations.
BIORESOURCE TECHNOLOGY
(2023)
Article
Microbiology
Justin P. Shaffer, Louis-Felix Nothias, Luke R. Thompson, Jon G. Sanders, Rodolfo A. Salido, Sneha P. Couvillion, Asker D. Brejnrod, Franck Lejzerowicz, Niina Haiminen, Shi Huang, Holly L. Lutz, Qiyun Zhu, Cameron Martino, James T. Morton, Smruthi Karthikeyan, Melissa Nothias-Esposito, Kai Duehrkop, Sebastian Boecker, Hyun Woo Kim, Alexander A. Aksenov, Wout Bittremieux, Jeremiah J. Minich, Clarisse Marotz, MacKenzie M. Bryant, Karenina Sanders, Tara Schwartz, Greg Humphrey, Yoshiki Vasquez-Baeza, Anupriya Tripathi, Laxmi Parida, Anna Paola Carrieri, Kristen L. Beck, Promi Das, Antonio Gonzalez, Daniel McDonald, Joshua Ladau, Soren M. Karst, Mads Albertsen, Gail Ackermann, Jeff DeReus, Torsten Thomas, Daniel Petras, Ashley Shade, James Stegen, Se Jin Song, Thomas O. Metz, Austin D. Swafford, Pieter C. Dorrestein, Janet K. Jansson, Jack A. Gilbert, Rob Knight
Summary: This study presents a multi-omics analysis framework for characterizing microbial communities across diverse habitats on Earth, demonstrating relationships between metabolites and microbial taxa and providing a reference database for future studies. The research showcases the significance of microbial and chemical ecology and lays a foundation for multi-omics microbiome studies.
NATURE MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Christian Fink, Gines Martinez-Cano, Jeremiah Shuster, Aurora Panzera, Kim E. Rennhack, Nils Rohbohm, Largus T. Angenent, Bastian Molitor
Summary: This study investigates the genetic basis and functional role of Mth60 fimbriae in Methanothermobacter thermautotrophicus Delta H. The researchers performed constitutive expression and deletion experiments of Mth60 fimbria-encoding genes in the organism. They found that overexpression increased the number of fimbriae, while deletion led to their loss in planktonic cells. The presence or absence of Mth60 fimbriae correlated with the formation of biotic cell-cell connections in the strains.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Editorial Material
Biochemical Research Methods
Ilje Pikaar, Tim Hulsen, Largus T. Angenent
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Microbiology
Andrew W. Grenfell, Peter J. Intile, John A. McFarlane, Dani C. Leung, Khalid Abdalla, Michael C. Wold, Eric D. Kees, Jeffrey A. Gralnick
Summary: Phages exert selective pressure on bacteria, influencing and shaping microbial populations. This study discovered a zebrafish-associated phage called Shewanella phage FishSpeaker, which uses outer membrane decaheme cytochrome OmcA and the flagellum to recognize and infect susceptible cells.
JOURNAL OF BACTERIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Farzaneh Pourmasoumi, Sundar Hengoju, Katharina Beck, Philipp Stephan, Lukas Klopfleisch, Maria Hoernke, Miriam A. Rosenbaum, Hajo Kries
Summary: This study establishes a microfluidic platform for reliable detection of nonribosomal peptide gramicidin S production and identification of NRPS mutants, providing a high-throughput method for studying large libraries of NRPS variants. The platform enables more sophisticated structure-activity studies and new engineering applications in the future.
Article
Microbiology
Madison J. Kalb, Andrew W. Grenfell, Abhiney Jain, Jane Fenske-Newbart, Jeffrey A. Gralnick
Summary: In this study, a recombineering workflow using five phage-encoded recombinases was developed to edit the genomes of three PGPR strains. By introducing point mutations, the maximum recombineering efficiencies were achieved. This work validates the potential of genetic manipulation in the Pseudomonas genus using these tools.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Isabella Casini, Tim Mccubbin, Sofia Esquivel-Elizondo, Guillermo G. Luque, Daria Evseeva, Christian Fink, Sebastian Beblawy, Nicholas D. Youngblut, Ludmilla Aristilde, Daniel H. Huson, Andreas Draeger, Ruth E. Ley, Esteban Marcellin, Largus T. Angenent, Bastian Molitor
Summary: This study investigates the metabolic mechanism of methane-producing archaea and reveals the impact of formate anabolism on carbon conversion, providing guidance for their application in power-to-gas technology and value-added chemical production.
Article
Biochemical Research Methods
Fengjie Zhao, Marko S. Chavez, Kyle L. Naughton, Christina M. Niman, Joshua T. Atkinson, Jeffrey A. Gralnick, Mohamed Y. El-Naggar, James Q. Boedicker
Summary: This study developed a facile technique for controlling the formation of electroactive biofilms by patterning conductive biofilms of Shewanella oneidensis through the regulation of aggregation protein expression. The technique has implications for both studying and harnessing bioelectronics.
ACS SYNTHETIC BIOLOGY
(2022)