Article
Engineering, Environmental
Xin Dong, Kentaro Yaji, Xiaomin Liu
Summary: This paper investigates the design of the mixing unit in a T-shape micromixer considering non-Newtonian fluid effects using topology optimization. The optimized structures exhibit superior performance in maximizing mixing quality, especially for non-Newtonian fluids.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Analytical
Yuchen Dai, Haotian Cha, Michael J. Simmonds, Hedieh Fallahi, Hongjie An, Hang T. Ta, Nam-Trung Nguyen, Jun Zhang, Antony P. McNamee
Summary: This study investigates the effects of altering fluid viscoelasticity on blood plasma extraction. Poly (ethylene oxide) (PEO) was added to increase blood viscoelasticity, resulting in improved cell focusing performance. The optimal PEO concentration was found to be in the range of 100 to 200 ppm, and the optimal flow rate was determined to be between 1 and 15 mu L/min.
Article
Chemistry, Analytical
Jianyu Ji, Shizhi Qian, Zhaohui Liu
Summary: This study numerically investigates the electroosmotic flow (EOF) of viscoelastic fluid and finds that under certain conditions, the EOF of viscoelastic fluid becomes unstable and vortices form at the upstream of the constriction. Compared to Newtonian fluid, the EOF velocity of viscoelastic fluid is spatially and temporally dependent, with the velocity at the exit much higher than the entrance.
Article
Chemistry, Analytical
Yao Chen, Lin Jiang, Xiaozhe Zhang, Zhonghua Ni, Nan Xiang
Summary: This article introduces an efficient label-free sorting and mechanical phenotyping method for tumor cells, which utilizes a viscoelastic-sorting integrated deformability cytometer (VSDC) to sort, extract, and analyze the mechanical properties of tumor cells. Machine learning algorithms are used to distinguish tumor cells from white blood cells.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Chemical
You Ma, Chunying Zhu, Taotao Fu, Youguang Ma, Huai Z. Li
Summary: The dynamics of non-Newtonian droplet breakup in a microfluidic Y-junction with partial obstruction was visually investigated. Three breakup stages were identified: squeezing, transition, and pinch-off. Factors such as the concentration of CMC, continuous capillary number, and dispersed phase local capillary number were found to affect the breakup process, with the pinch-off stage primarily determined by surface tension.
CHEMICAL ENGINEERING SCIENCE
(2021)
Article
Mathematics
Gerasim Vladimirovich Krivovichev
Summary: The paper compares different one-dimensional models of blood flow, taking into account the non-Newtonian property of blood. It analytically solves the simplified nonlinear problem for a semi-infinite vessel with constant properties and compares the solutions for different models. The effects of velocity profile flattening and hematocrit value on deviation from the Newtonian model are investigated.
Article
Chemistry, Analytical
Anoshanth Jeyasountharan, Francesco Del Giudice
Summary: The study investigates the encapsulation of particles in a microfluidic device using a non-Newtonian viscoelastic solution. The research reveals that the droplet formation mechanism for non-Newtonian solutions follows the same behavior as Newtonian solutions. It also identifies experimental conditions where the encapsulation efficiency of particles exceeds the stochastic limit predicted by Poisson statistics.
Article
Chemistry, Analytical
Jinhyeuk Song, Jaekyeong Jang, Taehoon Kim, Younghak Cho
Summary: This study investigates particle separation in a T-shaped microchannel and finds that a Newtonian/viscoelastic co-flow system effectively separates particles based on size compared to a Newtonian/Newtonian system. Increasing the flow rate ratio enhances the separation efficiency in the Newtonian/viscoelastic co-flow configuration.
Article
Engineering, Chemical
Bimalendu Mahapatra, Aditya Bandopadhyay
Summary: Geometrically modified microchannels fabricated using stereolithography are used to study micromixing of polymeric solutions. Experimental and numerical analyses were conducted to evaluate the validity of Newtonian fluid flow in these channels. The presence of inertio-elastic instability in the geometrically modified microchannels for viscoelastic fluid transport is observed, which significantly enhances the mixing efficiency. This analysis provides important insights for the cost-effective design and operation of microfluidic devices handling viscoelastic fluids, particularly in designing passive micromixers for bio/polymeric fluids transportation.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Chemistry, Physical
Sumit Kumar Mehta, Bappa Mondal, Sukumar Pati, Promod Kumar Patowari
Summary: This study investigates the mixing and hydrodynamic characteristics of a pure electroosmotic flow of non-Newtonian fluid through a nonuniformly charged micromixer with obstacles arranged in staggered and inline orders. The results show that the rheology of the fluids and the formation of recirculatory zones strongly influence the mixing efficiency. The presence of heterogeneous charged surfaces enhances the mixing, especially for shear-thickening fluids and staggered arrangements of obstacles.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Chemistry, Multidisciplinary
Seyong Kwon, Jieung Oh, Min Seok Lee, Eujin Um, Joonwoo Jeong, Joo H. Kang
Summary: A hemolysis-free and highly efficient plasma separation platform has been developed using enhanced diamagnetic repulsion of blood cells. By supplementing blood with superparamagnetic iron oxide nanoparticles, complete removal of blood cells from blood plasma is achieved, allowing for successful collection of plasma without losing plasma proteins, platelets, or exosomes.
Article
Chemistry, Analytical
Hyunjung Lim, Jae Young Kim, Seunghee Choo, Changseok Lee, Byoung Joe Han, Chae Seung Lim, Jeonghun Nam
Summary: This study demonstrates viscoelastic microfluidic techniques for continuous separation, concentration, and subsequent washing of Candida cells in the blood. Candida cells were successfully separated from the white blood cells and concentrated by 74.6-fold. Finally, Candida cells at extremely low concentrations became detectable after the removal of white blood cells, buffer solution, and further removal of blood lysate and washing.
Article
Polymer Science
Meng Zhang, Zihuang Wang, Yanhua Zheng, Bifeng Zhu, Bingzhi Zhang, Xiaohui Fang, Wenli Shang, Wu Zhang
Summary: This paper reports on the manipulation of elastic instability of a viscoelastic fluid in a rhombus cross microchannel structure. The study demonstrates bistable and unsteady instabilities of flow in a standard cross microchannel for reference. It is found that the stabilizing effect of the rhombus structure enables the maintenance of bistable instability over a wider range of injection rates in the rhombus cross microchannel. Additionally, a semi-bistable instability is established at high injection rates. Moreover, unsteady elastic instability is achieved in the rhombus cross microchannel through asymmetric injection rate conditions.
Article
Chemistry, Multidisciplinary
Yanfang Guan, Xiaoliang Wang, Guangyu Liu, Wujie Li, Kun Zhang, Baoshuo Sun, Feifan Shi, Yanbo Hui, Bingsheng Yan, Jie Xu, Zaihui Wu, Zhiyong Duan, Ronghan Wei
Summary: This study introduces 2D and 3D platforms driven by BAW and LCBE in liquid crystal solutions for efficient microparticle manipulation, allowing for precise control of microparticles and alteration of their moving direction.
Article
Mechanics
Uddipta Ghosh, Siddhartha Mukherjee, Suman Chakraborty
Summary: The study analyzes the electrophoretic motion of non-uniformly charged particles in a viscoelastic medium, revealing significant differences in their behavior compared to Newtonian fluids. It shows that the particle's velocity strongly depends on its size and surface charge distribution in a viscoelastic medium, contrary to the established theory for weakly charged particles in Newtonian fluids. The presence of non-uniform surface charge enhances the influence of the medium's viscoelasticity on the particle's translational and angular velocities, with the effect depending strongly on the nature of surface charge distribution.
JOURNAL OF FLUID MECHANICS
(2021)
Article
Oncology
Yuri Belotti, Elaine Hsuen Lim, Chwee Teck Lim
Summary: In this study, a 19-gene TME-related HGSOC prognostic genetic panel was identified using bioinformatics methods, and an association between tumor-infiltrating immune cells and mortality rates was found.
Review
Cell Biology
Jia Mei Hong, Hyeyoung Lee, Nishanth Menon, Chwee Teck Lim, Luke P. Lee, Catherine W. M. Ong
Summary: This review evaluates the current diagnostic assays endorsed by the World Health Organization for tuberculosis and discusses the development of rapid tests and microfluidics technology.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Oncology
Yuri Belotti, Serenella Tolomeo, Rongjun Yu, Wan-Teck Lim, Chwee Teck Lim
Summary: This study investigates the role of dysregulated neurotransmitter receptors (NTRs) genes in the prognosis of low-grade glioma (LGG) and glioblastoma multiforme (GBM). The findings suggest that certain NTR genes are associated with different patient survival outcomes in LGG and GBM. These genes show downregulation across glioma grades and have a negative correlation with genes related to immune response, inflammation, and cancer hallmarks in LGG. The study highlights the potential implications for therapeutic and preventive strategies targeting the regulatory networks involving the autonomic nervous system, cancer cells, and the tumor microenvironment.
Article
Chemistry, Analytical
Seunghee Choo, Hyunjung Lim, Tae Eun Kim, Jion Park, Kyu Been Park, Chaewon Park, Chae Seung Lim, Jeonghun Nam
Summary: Water contamination is a critical global public health issue. This study presents a viscoelastic microfluidic device for the continuous concentration of bacterial cells, aiming to address the limitations of current detection methods. The device was validated for cell concentration by optimizing flow characteristics and achieving highly concentrated bacterial cells.
Article
Chemistry, Multidisciplinary
Yufeng Shou, Xin Yong Teo, Xianlei Li, Zhicheng Le, Ling Liu, Xinhong Sun, Win Jonhson, Jun Ding, Chwee Teck Lim, Andy Tay
Summary: This study developed a dynamic 3D magnetic hydrogel that can wirelessly and reversibly change its matrix stiffness multiple times using an external magnet, enabling the study of the impact of matrix softening on cancer biology. It was found that increasing matrix stiffness promotes tumor malignancy, but the malignant transformations can be halted or reversed by matrix softening, enhancing drug efficacy.
Article
Chemistry, Multidisciplinary
Shuwen Chen, Shicheng Fan, Jiaming Qi, Ze Xiong, Zheng Qiao, Zixiong Wu, Joo Chuan Yeo, Chwee Teck Lim
Summary: A bilayer liquid-solid conductor (b-LSC) with amphiphilic properties is introduced in this study to interface reliably with both rigid electronics and elastomeric substrates. It exhibits high strain-insensitive conductivity, extreme stretchability, and negligible resistance change under ultra-high strain. The b-LSC can be used for various applications, such as stretchable sensor arrays, multi-layered stretchable displays, and highly integrated haptic user-interactive optoelectric E-skins.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Analytical
Huanqian Zhang, Jee Chin Teoh, Jianfeng Wu, Longteng Yu, Chwee Teck Lim
Summary: One advantage of a resistive sensor array (RSA) with shared rows and shared columns is the reduced number of wires, which can lessen the complexity and burden on wearable electronic systems. However, the drawback is the crosstalk current effect between adjacent elements, which leads to high measurement error. This study introduces a new circuit design called the dynamic zero current method (DZCM) to decrease the measurement error further.
Article
Engineering, Biomedical
Hardik Makkar, Ying Zhou, Kai Soo Tan, Chwee Teck Lim, Gopu Sriram
Summary: Gingival crevice and gingival crevicular fluid (GCF) flow are crucial for maintaining gingival health and preventing periodontal disease. However, current in vitro models lack the dynamic nature of the gingival crevicular microenvironment, hindering the development of periodontal therapeutics. This study describes a microfluidic platform that mimics GCF flow to culture gingival connective tissue. The platform successfully replicated microbial colonization, biofilm formation, and early gingival inflammation in response to oral symbiont and periodontal pathogen exposure. The induction of flow attenuated the secretion of inflammatory mediators, demonstrating the protective effect of GCF flow.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Y. Belotti, D. S. Jokhun, V. L. M. Valerio, T. W. Chong, C. T. Lim
Summary: We developed a fast and cost-effective method that combines pH imaging and machine learning for non-invasive identification of different cell populations at the single-cell level. By leveraging biophysical and biochemical markers obtained from pH imaging with deep learning, we achieved highly accurate classification of cancer cells.
Article
Chemistry, Analytical
Hyunjung Lim, Jae Young Kim, Seunghee Choo, Changseok Lee, Byoung Joe Han, Chae Seung Lim, Jeonghun Nam
Summary: This study demonstrates viscoelastic microfluidic techniques for continuous separation, concentration, and subsequent washing of Candida cells in the blood. Candida cells were successfully separated from the white blood cells and concentrated by 74.6-fold. Finally, Candida cells at extremely low concentrations became detectable after the removal of white blood cells, buffer solution, and further removal of blood lysate and washing.
Review
Nanoscience & Nanotechnology
Christy Kwokdinata, Vaibavi Ramanujam, Jiahui Chen, Paula Nunes de Oliveira, Mui Hoon Nai, Wai Hon Chooi, Chwee Teck Lim, Shi Yan Ng, Laurent David, Sing Yian Chew
Summary: This study found that using injectable hyaluronan-gelatin hydrogel in transplantation therapy can promote cell survival and neural differentiation of human spinal cord progenitor cells.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Biomedical
Hardik Makkar, Chwee Teck Lim, Kai Soo Tan, Gopu Sriram
Summary: Gingival connective tissue and its vasculature are important for the immune response against oral bacteria and act as a connection between the oral and systemic environments. However, there's a lack of models that mimic the complex features of vascularized gingival connective tissue and its interaction with oral bacteria, limiting our understanding of periodontal health and disease. In this study, the researchers developed vascularized gingival tissue models to study the interactions between oral bacteria and gingival tissues in different states. They found that different oral bacteria had different effects on the tissue, with some leading to disease symptoms. The models also showed potential for studying periodontitis and vascular diseases. Overall, the vascularized gingival tissue models provide an in vitro platform for studying host-microbiome interactions and immune response in periodontal health and disease, and may contribute to the development of new periodontal therapeutics.
Article
Chemistry, Analytical
Hyunjung Lim, Minji Kim, Yeongmu Kim, Seunghee Choo, Tae Eun Kim, Jaesung Han, Byoung Joe Han, Chae Seung Lim, Jeonghun Nam
Summary: This study demonstrates a continuous on-chip washing process using a co-flow system with viscoelastic and Newtonian fluids, addressing limitations of centrifugation-based medium exchange. It also showcases the application of on-chip washing for white blood cells (WBCs) in lysed blood samples.
Article
Chemistry, Multidisciplinary
Jiaming Qi, Feng Gao, Guanghui Sun, Joo Chuan Yeo, Chwee Teck Lim
Summary: To enhance the realism and immersion of human-computer interaction, novel haptics technologies are developed to bridge the gap between the physical world and digital environment. The HaptGlove, an untethered and lightweight pneumatic glove, allows users to physically interact in a VR environment, providing natural and realistic kinesthetic and cutaneous sensations. Through a user study, significant improvements in VR realism and immersion are observed, with participants achieving 78.9% accuracy in sorting virtual objects of different stiffnesses. Importantly, HaptGlove facilitates VR training, education, entertainment, and socialization.
Review
Chemistry, Multidisciplinary
Shuwen Chen, Shicheng Fan, Henryk Chan, Zheng Qiao, Jiaming Qi, Zixiong Wu, Joo Chuan Yeo, Chwee Teck Lim
Summary: Flexible wearable devices and medical robotics are crucial for smart healthcare. Liquid metal materials have emerged as key elements for these innovations. This review discusses the latest breakthroughs in liquid metal functionalization and their applications in healthcare-related wearables and soft robotics.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
