Optimizing methodologies for PCR-based DNA methylation analysis

Methylation profiling of normal individuals reveals mosaic promoter methylation of cancer-associated genes.

(2015) Lasse S. Kristensen et al.   Oncotarget

Primer design versus PCR bias in methylation independent PCR amplifications

(2014) Tomasz K Wojdacz et al.   Epigenetics

Genome-Wide Identification and Validation of a Novel Methylation Biomarker, SDC2, for Blood-Based Detection of Colorectal Cancer

(2013) TaeJeong Oh et al.   JOURNAL OF MOLECULAR DIAGNOSTICS

BioWord: A sequence manipulation suite for Microsoft Word

(2012) Laura J Anzaldi et al.   BMC BIOINFORMATICS

Comparative assessment of 5 methods (methylation-specific polymerase chain reaction, methylight, pyrosequencing, methylation-sensitive high-resolution melting, and immunohistochemistry) to analyze O6-methylguanine-DNA-methyltranferase in a series of 100 g

(2012) Véronique Quillien et al.   CANCER

Peripheral blood DNA methylation profiles are indicative of head and neck squamous cell carcinoma: An epigenome-wide association study

(2012) Scott M. Langevin et al.   Epigenetics

DNA methylation profiling in the clinic: applications and challenges

(2012) Holger Heyn et al.   NATURE REVIEWS GENETICS

DNA methylation in neurodegenerative disorders: a missing link between genome and environment?

(2011) S Iraola-Guzmán et al.   CLINICAL GENETICS

Poor reliability and reproducibility of PCR-based testing of O6-methylguanine-DNA methyltransferase gene (MGMT) promoter methylation status in formalin-fixed and paraffin-embedded neurosurgical biopsy specimens

(2011) M. Preusser et al.   CLINICAL NEUROPATHOLOGY

Java web tools for PCR, in silico PCR, and oligonucleotide assembly and analysis

(2011) Ruslan Kalendar et al.   GENOMICS

Advances in genome-wide DNA methylation analysis

(2010) Romi Gupta* et al.   BIOTECHNIQUES

BISMA - Fast and accurate bisulfite sequencing data analysis of individual clones from unique and repetitive sequences

(2010) Christian Rohde et al.   BMC BIOINFORMATICS

Quantitative assessment of AKAP12 promoter methylation in colorectal cancer using methylation-sensitive high resolution melting: Correlation with Duke's stage

(2010) Weiwei Liu et al.   CANCER BIOLOGY & THERAPY

Development of a multiplex MethyLight assay for the detection of multigene methylation in human colorectal cancer

(2010) Qiong He et al.   CANCER GENETICS AND CYTOGENETICS

Hypermethylated DAPK in serum DNA of women with uterine leiomyoma is a biomarker not restricted to cancer

(2010) Norman Häfner et al.   GYNECOLOGIC ONCOLOGY

Methylation of MGMT in malignant pleural mesothelioma occurs in a subset of patients and is associated with the T allele of the rs16906252 MGMT promoter SNP

(2010) Lasse Sommer Kristensen et al.   LUNG CANCER

Epigenetic modifications and human disease

(2010) Anna Portela et al.   NATURE BIOTECHNOLOGY

Principles and challenges of genome-wide DNA methylation analysis

(2010) Peter W. Laird   NATURE REVIEWS GENETICS

Lasting Epigenetic Influence of Early-Life Adversity on the BDNF Gene

(2009) Tania L. Roth et al.   BIOLOGICAL PSYCHIATRY

Verification of complete bisulfite modification using Calponin-specific primer sets

(2009) Ruethairat Sriraksa et al.   CLINICAL BIOCHEMISTRY

High Quality Assessment of DNA Methylation in Archival Tissues from Colorectal Cancer Patients Using Quantitative High-Resolution Melting Analysis

(2009) Marija Balic et al.   JOURNAL OF MOLECULAR DIAGNOSTICS

Rapid quantification of DNA methylation by measuring relative peak heights in direct bisulfite-PCR sequencing traces

(2009) Minghong Jiang et al.   LABORATORY INVESTIGATION

The human colon cancer methylome shows similar hypo- and hypermethylation at conserved tissue-specific CpG island shores

(2009) Rafael A Irizarry et al.   NATURE GENETICS

Methylation-sensitive high-resolution melting

(2009) Tomasz K Wojdacz et al.   Nature Protocols

MethMarker: user-friendly design and optimization of gene-specific DNA methylation assays

(2009) Peter Schüffler et al.   GENOME BIOLOGY

Single-molecule polymerase chain reaction reduces bias: Application to DNA methylation analysis by bisulfite sequencing

(2008) Aparna Chhibber et al.   ANALYTICAL BIOCHEMISTRY

Promoting coherent minimum reporting guidelines for biological and biomedical investigations: the MIBBI project

(2008) Chris F Taylor et al.   NATURE BIOTECHNOLOGY

DNA Methylation Markers and Early Recurrence in Stage I Lung Cancer

(2008) Malcolm V. Brock et al.   NEW ENGLAND JOURNAL OF MEDICINE

DNA methylation analysis by digital bisulfite genomic sequencing and digital MethyLight

(2008) D. J. Weisenberger et al.   NUCLEIC ACIDS RESEARCH

Sensitive Melting Analysis after Real Time- Methylation Specific PCR (SMART-MSP): high-throughput and probe-free quantitative DNA methylation detection

(2008) L. S. Kristensen et al.   NUCLEIC ACIDS RESEARCH

Rapid analysis of heterogeneously methylated DNA using digital methylation-sensitive high resolution melting: application to the CDKN2B (p15) gene

(2008) Ida LM Candiloro et al.   Epigenetics & Chromatin