Journal
BIOTECHNIQUES
Volume 51, Issue 5, Pages 335-+Publisher
BIOTECHNIQUES OFFICE
DOI: 10.2144/000113763
Keywords
BAC DNA; transgenesis; RECOCHIP; Electro-elution
Funding
- National Institutes of Health (NIH)
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An unresolved bottleneck in bacterial artificial chromosome (BAG) transgenesis is low efficiency generation of founder mice because of suboptimal quality of the manipulated BAG DNA. Using mini-gel electrophoresis and electro-elution that circumvents CsCl(2) centrifugation, column chromatography, and resin purifications, we have used RECOCHIP, a commercially available dialysis cassette for the purification of BAG DNA that generates transgenic founders with up to 80% efficiency.
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