期刊
BIOTECHNIQUES
卷 51, 期 5, 页码 335-+出版社
BIOTECHNIQUES OFFICE
DOI: 10.2144/000113763
关键词
BAC DNA; transgenesis; RECOCHIP; Electro-elution
资金
- National Institutes of Health (NIH)
An unresolved bottleneck in bacterial artificial chromosome (BAG) transgenesis is low efficiency generation of founder mice because of suboptimal quality of the manipulated BAG DNA. Using mini-gel electrophoresis and electro-elution that circumvents CsCl(2) centrifugation, column chromatography, and resin purifications, we have used RECOCHIP, a commercially available dialysis cassette for the purification of BAG DNA that generates transgenic founders with up to 80% efficiency.
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