Article
Biophysics
Silio Lima Moura, Arnau Pallares-Rusinol, Luciano Sappia, Merce Marti, Maria Isabel Pividori
Summary: This article introduces a biosensor combining immunomagnetic separation and electrochemical biosensing based on the intrinsic ALP activity of exosomes. By improving the analytical performance for detecting ALP activity in exosomes, reliable differentiation between breast cancer patients and healthy individuals is achieved.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemical Research Methods
Preeyanut Butmee, Gamolwan Tumcharern, Chomphunuch Songsiriritthigul, Marie Jose Durand, Gerald Thouand, Margaret Kerr, Kurt Kalcher, Anchalee Samphao
Summary: This study fabricated a novel enzymatic electrochemical biosensor for detecting glyphosate-based acid phosphatase inhibition. The biosensor functioned by measuring oxidation current through enzymatic reactions, and was successfully applied for selective analysis of glyphosate in actual samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Fuchun Si, Yaping Zhang, Jing Lu, Mengyuan Hou, Huaixia Yang, Yanju Liu
Summary: Alkaline phosphatase (ALP) is an important enzyme with significant implications in biomedical research and clinical diagnosis. A novel electrochemical biosensor based on photoinduced atom transfer radical polymerisation (photoATRP) has been developed for the analysis of ALP. This biosensor demonstrates a wide linear range, low detection limits, and excellent selectivity and interference resistance in human serum samples.
Article
Biochemical Research Methods
Wenbin Wang, Jing Lu, Lulu Hao, Huaixia Yang, Xuejie Song, Fuchun Si
Summary: A novel electrochemical method utilizing aminoferrocene as an electroactive probe for alkaline phosphatase (ALP) activity detection was described in this study. The approach demonstrated good linear relationship and sensitivity in detecting ALP activity, with high selectivity and applicability.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Review
Biochemical Research Methods
Patrick Severin Sfragano, Serena Pillozzi, Gerolama Condorelli, Ilaria Palchetti
Summary: In order to address cancer-related issues, the biosensing field is constantly searching for new biomarkers that can be non-invasively analyzed. Extracellular vesicles (EVs) have emerged as promising biomarkers that can provide direct information on diseases. However, there is a lack of practical and effective quantitative procedures for EV detection due to difficulties in the pre-analytical and analytical phases. This review aims to provide a comprehensive overview of recent progress in EV detection using electrochemical and photoelectrochemical biosensors.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Lin Cui, Chen-yu Zhu, Juan Hu, Xiao-meng Meng, Mengmeng Jiang, Wenqiang Gao, Xiaolei Wang, Chun-yang Zhang
Summary: A dual-mode biosensor based on fully-pi conjugated covalent organic framework (COF) and cobalt oxyhydroxide (CoOOH) nanoflakes is demonstrated for electrochemiluminescent (ECL) and electrochemical sensing of alkaline phosphatase (ALP). This dual-mode biosensor can efficiently eliminate errors and provide more reliability and accuracy than a single-mode biosensor.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Sara Ranjbari, Majid Rezayi, Reza Arefinia, Seyed Hamid Aghaee-Bakhtiari, Behnaz Hatamluyi, Alireza Pasdar
Summary: In this study, a novel electrochemical biosensor amplified with hierarchical flower-like gold, poly(n-butyl acrylate), and MXene nanocomposite was developed for the detection of miRNA-122 as a biomarker of breast cancer.
Article
Chemistry, Analytical
Eleni Koukouviti, Christos Kokkinos
Summary: The study presents a novel miniature all-3D-printed 4-electrode biochip capable of simultaneous determination of different biomarkers, showcasing high sensitivity and transferability.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Ali Khodadoust, Navid Nasirizadeh, Seyed Morteza Seyfati, Ramezan Ali Taheri, Mostafa Ghanei, Hasan Bagheri
Summary: In this study, a novel nano-biosensor has been developed using dual signal-labeled hairpin-structured DNA-based probes. The biosensor can sensitively and simultaneously detect miRNA-141 and miRNA-21, and it is resistant to interference from environmental factors.
Review
Chemistry, Analytical
Linlin Wang, Wenlei Zhu, Jianrong Zhang, Jun-Jie Zhu
Summary: Electrochemical biosensors, utilizing enzymatic biofuel cells as energy and signal generators, have become a research hotspot due to their power self-support and simplified structure. Enzymatic biofuel cell-powered biosensors (EBFC-SPBs) have achieved tremendous success in health monitoring, but the miniaturization of portable EBFC-SPBs remains challenging. Microfluidic technology has significantly contributed to the progress of miniaturized and portable EBFC-SPBs. This review summarizes the achievements and progress in miniaturized EBFC-SPBs and discusses the advantages and challenges of microfluidic and screen-printing technologies for wearable and disposable EBFC-SPBs.
Article
Biochemistry & Molecular Biology
Jianhua Yan, Kaidi Wang, Hongjie Liu, Liwei Wang, Yixiang Li, Guoqing Zhang, Li Deng
Summary: In this study, an electrochemical biosensor was developed based on the flower-like MoSe2@1T-MoS2 heterojunction electrode material and specific RNA recognition probes for rapid detection and quantification of miRNA-155 in breast cancer. The biosensor exhibited a wide detection range of 1 fM to 1 nM with a low limit of detection (LOD) of 0.34 fM. The biosensor accurately measured the miRNA-155 content in blood samples of tumor-bearing mice and normal mice, demonstrating its strong identification ability and potential as a diagnostic marker. A portable sensor platform was also designed for on-site detection simulation, which showed good recovery efficiency and verified the accuracy and reliability of the biosensor compared to the standard detection method RT-qPCR, suggesting its potential for point-of-care testing (POCT) in early breast cancer diagnosis.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Qingyu Wang, Haobo Sun, Dongxiao Wen, Lei Wang, Lianzhi Li, Jinming Kong, Xueji Zhang
Summary: An electrochemical sensing platform based on ATRP signal amplification strategy has been developed for ultrasensitive determination of breast and prostate cancer marker miRNA-141, with a linear range from 10 pM to 10 aM and a detection limit of 3.23 aM, providing a new and promising path for ultrasensitive analysis of tumor-related miRNAs.
Article
Chemistry, Analytical
Xiao-Yan Yang, Yi-Yan Bai, Yue-Yue Huangfu, Wen-Jing Guo, Yan-Ju Yang, Dai-Wen Pang, Zhi-Ling Zhang
Summary: An ultrasensitive electrochemiluminescence biosensor based on a closed bipolar electrode was proposed for the detection of alkaline phosphatase. The sensitivity of the system was greatly enhanced by utilizing enzyme catalysis for signal amplification and electrodeposition of Au nanoparticles on the cathode surface. This biosensor showed high sensitivity and specificity, with a detection limit as low as 3.7 aM, and successfully realized ALP detection in single cells.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Zahra Mohammadpour, Mojtaba Kamankesh, Mahdieh Shokrollahi Barough, Tavia Walsh, Sadegh Ghorbanzadeh, David Hamdi, Mohsen Akbari, Yasser Zare, Kyong Yop Rhee, Amir Seyfoori
Summary: Polymer and nanosized objects integration in electrochemical biosensors has been shown to enhance the analysis of breast cancer biomarkers. Recent developments have demonstrated improved sensitivity and selectivity, as well as signal amplification through the incorporation of nanocomposites. Polymer-based nanocomposites in electrochemical biosensors have the potential to revolutionize disease management by enabling user-friendly breast cancer screening and early detection.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Li Hou, Xuanhan Zhang, Yuxiu Huang, Min Wang, Xiaoyu Chen, Tianran Lin, Yanhui Tan, Shulin Zhao
Summary: A sensitive and ratiometric electrochemical biosensor was developed for the determination of dimethoate via alkaline phosphatase (ALP) mediated dissolution of nano-MnO2 and [Ru(NH3)(6)](3+)(Ru(III)) redox recycling. The biosensor showed good performance in detecting dimethoate with high sensitivity and a detection limit of 6.3 pg mL(-1).
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Huseyin Oguzhan Kaya, Merve Karpuz, Seda Nur Topkaya
Summary: In this study, the electrochemical quantification of CMS released from liposomes was achieved for the first time. The cationic liposome showed higher encapsulation efficiency and controlled release with zero-order drug release kinetics.
Article
Biochemistry & Molecular Biology
Emre Gezer, Goklem Uner, Melis Kucuksolak, Mustafa Unver Kurt, Gamze Dogan, Petek Ballar Kirmizibayrak, Erdal Bedir
Summary: Polyether ionophores are a group of naturally occurring compounds mainly produced by Streptomyces species with various bioactivities. In this study, four undescribed and nine known polyether compounds were identified from Streptomyces cacaoi. The antimicrobial activity assays showed strong inhibitory effects of these compounds, including an undescribed compound, on the growth of Bacillus subtilis and methicillin-resistant Staphylococcus aureus (MRSA). Additionally, two compounds displayed significant cytotoxicity, with the C15-demethoxy derivative showing higher selectivity towards cancer cells than K41-A. Mechanistic experiments revealed that the uninvestigated compound acts as a multitarget compound, inhibiting autophagic flux, inducing reactive oxygen species formation, abolishing proteasome activity, and stimulating ER stress.
Article
Biochemistry & Molecular Biology
Sinem Yilmaz, Erdal Bedir, Petek Ballar Kirmizibayrak
Summary: The small molecule telomerase activator Cycloastragenol (CA) has been found to have a positive impact on age-related pathophysiologies by increasing NRF2 activity, regulating telomerase activity, and enhancing proteasome activity. These findings suggest that CA plays a role in multiple cellular pathways associated with aging.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Multidisciplinary Sciences
Goklem Uner, Erdal Bedir, Onur Sercinoglu, Petek Ballar Kirmizibayrak
Summary: The discovery of novel chemotherapeutics that act through different mechanisms is crucial for addressing tumor heterogeneity and therapeutic resistance. This study further elucidates the unique cell death mechanism of AG-08 and its ability to form supramolecular particles. Through investigation of 18 analogs, the structural features required for the formation of particulate structures and stimulation of AG-08's unprecedented cell death mechanism were determined.
SCIENTIFIC REPORTS
(2022)
Review
Chemistry, Analytical
Dilsat Ozkan-Ariksoysal
Summary: Since the introduction of the first commercial biosensor for blood glucose measurement in the 1970s, many types of biosensors have been developed, and this field of research remains popular globally. It is predicted that in the near future, biosensor design technologies such as handheld or wearable devices will be preferred and highly valuable in various fields. Nanoparticles continue to play a crucial role in science and technology, with carbon-based nanoparticles, especially graphene oxide, being considered one of the most valuable nanomaterials in electrochemical biosensors. This review summarizes recent developments using graphene oxide-based nanomaterials in biosensor technologies for cancer diagnosis.
Review
Medicine, General & Internal
Mohamad Ahmad Najib, Kasturi Selvam, Muhammad Fazli Khalid, Mehmet Ozsoz, Ismail Aziah
Summary: Infectious diseases are the leading cause of death worldwide. As the risk of emerging diseases increases, it is crucial to quickly diagnose infections and prevent further transmission. Current detection methods are time-consuming and less sensitive, while point-of-care testing offers the advantages of being rapid, user-friendly, and highly sensitive and specific. However, conventional detection methods still face the challenge of low sensitivity.
Review
Medicine, General & Internal
Nor Syafirah Zambry, Mohamad Ahmad Najib, Mohd Syafiq Awang, Kasturi Selvam, Muhammad Fazli Khalid, Yazmin Bustami, Hairul Hisham Hamzah, Mehmet Ozsoz, Asrulnizam Abd Manaf, Ismail Aziah
Summary: This scoping review assesses the current trends in electrochemical aptasensors for detecting and quantifying Salmonella. The review found that electrochemical aptasensors offer simplicity, on-site quantitative analysis, rapid detection time, high sensitivity, and portability. The developed aptasensors mainly target Salmonella enterica Typhimurium in chicken meat samples, with detection times ranging from 5 minutes to 240 minutes and detection limits from 550 CFU/mL to as low as 1 CFU/mL. The promising performance of electrochemical aptasensors highlights their potential as excellent alternatives to existing detection methods, but further research is needed to determine their sensitivity and specificity, particularly in human clinical samples.
Article
Biochemistry & Molecular Biology
Irem Tatar, Sirin Uysal, Sinem Yilmaz, Ayse H. Tarikogullari, Petek Ballar Kirmizibayrak, Zeynep Soyer
Summary: A series of novel noncovalent glycine/beta-alanine anilide derivatives with 2-chloronaphthoquinone structure were designed, synthesized, and evaluated for their antiproliferative and antiproteasomal activities against MCF-7 cell line. The target compounds showed antiproliferative activity in the range of IC50 = 7.10 +/- 0.10-41.08 +/- 0.14 μM and exhibited inhibitory efficacy against the catalytic subunits of proteasome. Among the compounds, compounds 7 and 9 showed the highest antiproliferative activity, and compound 7 displayed comparable potency in terms of beta 5 antiproteasomal activity.
CHEMICAL BIOLOGY & DRUG DESIGN
(2023)
Article
Biotechnology & Applied Microbiology
Melis Kucuksolak, Sinem Yilmaz, Petek Ballar-Kirmizibayrak, Erdal Bedir
Summary: In this study, a new endophytic fungus, Camarosporium laburnicola, was found to catalyze the synthesis of new metabolites from Astragalus angustifolius, which are potential telomerase activators. Seven new metabolites were obtained and characterized through biotransformation, demonstrating the potential of C. laburnicola as a biocatalyst.
MICROBIAL CELL FACTORIES
(2023)
Article
Cell Biology
Esra Atalay Sahar, Petek Ballar Kirmizibayrak
Summary: The heterogeneity of cancer necessitates the exploration of alternative pathways for targeting. Endoplasmic reticulum stress-related pathways, such as ERAD, which mediates proteasome-dependent degradation of unfolded proteins, are gaining attention as potential new anticancer treatments. SVIP, an endogenous ERAD inhibitor, has been found to play a role in cancer progression, particularly in glioma, prostate, and head and neck cancers. In this study, the expression and function of the SVIP gene in breast cancer was evaluated using RNA-seq and gene array studies, revealing differential expression patterns and potential interactions with the tumor suppressor protein p53.
Article
Chemistry, Analytical
Yeni Wahyuni Hartati, Irkham Irkham, Iis Sumiati, Santhy Wyantuti, Shabarni Gaffar, Salma Nur Zakiyyah, Muhammad Ihda H. L. Zein, Mehmet Ozsoz
Summary: Fast, sensitive, and easy-to-use label-free electrochemical DNA biosensor method was developed for the detection of pork in processed meat samples. The method utilized gold electrodeposited screen-printed carbon electrodes and differential pulse voltammetry for probe-target DNA hybridization detection. The detection limit was 0.135 μg/mL, and the method showed selectivity against 5% pork DNA in a mixture of meat samples. This electrochemical biosensor method has the potential to be developed into a portable point-of-care detection method for pork or food adulterations.
Article
Chemistry, Analytical
Suzan Yanik, Deniz Emre, Meltem Alp, Fatih Algi, Selehattin Yilmaz, Ali Bilici, Dilsat Ozkan-Ariksoysal
Summary: The study demonstrates the performance of a newly developed nanomaterial-based biosensor for the analysis of drug-DNA interaction. The nanomaterial-modified sensor showed significantly higher sensitivity compared to the bare electrode. The developed nanobiosensor system provided highly specific determination between the anticancer drug Mitomycin C and the drug Acyclovir by discriminating their interactions with double-stranded DNA.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Engineering, Electrical & Electronic
Sena Yaman, Meryem Beyza Avci, Fatma Kurul, Seda Nur Topkaya, Arif E. Cetin
Summary: We have developed a lensfree optofluidic platform for label-free identification of biomolecular interactions. Our platform is compact, portable, and utilizes microfluidics and on-chip computational imaging. The experimental results demonstrate the high sensing capabilities and cost-effective infrastructure of our platform, making it a promising tool for biosensing applications.
SENSORS AND ACTUATORS A-PHYSICAL
(2023)
Proceedings Paper
Engineering, Biomedical
Fatma Kurul, Ziya Ata Yazici, Zeynep A. Kocer, Seda Nur Topkaya, Arif E. Cetin
Summary: The development of rapid diagnostic kits is crucial for early diagnosis and treatment of infectious diseases. This study introduces a lightweight and portable biosensor that utilizes a plasmonic chip based on nanohole arrays integrated into a lens-free imaging system for label-free virus detection in field settings. The biosensor platform employs a high-efficiency CMOS camera to observe the diffraction field patterns of nanohole arrays, enabling reliable and accurate detection of H1N1 viruses at medically relevant concentrations. The platform technology is adaptable and can be used for detecting other viral diseases such as COVID-19 or influenza by modifying the plasmonic chip surface.
TRANSLATIONAL BIOPHOTONICS: DIAGNOSTICS AND THERAPEUTICS III
(2023)
Proceedings Paper
Engineering, Biomedical
Meryem Beyza Avci, Seda Nur Topkaya, Ozden Yalcin-Ozuysal, Ali Khademhosseini, Arif E. Cetin
Summary: A new platform has been developed to measure the weight and growth of individual cells in real time, allowing for quick measurement of cell growth in small samples and determination of differences in cell population growth and cancer cells' response to drug treatment.
TRANSLATIONAL BIOPHOTONICS: DIAGNOSTICS AND THERAPEUTICS III
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
