Review
Biotechnology & Applied Microbiology
Muneyoshi Kanai, Masaki Mizunuma, Tsutomu Fujii, Haruyuki Iefuji
Summary: Sake yeasts possess specific brewing characteristics that are beneficial for sake making, and they also accumulate various functional components such as S-adenosylmethionine (SAM) and folate. Understanding the mechanisms of these components is crucial for fully characterizing "sake yeast identity" and improving the sake brewing process.
FEMS YEAST RESEARCH
(2023)
Article
Microbiology
Norapat Klinkaewboonwong, Shinsuke Ohnuki, Tomoya Chadani, Ikuhisa Nishida, Yuto Ushiyama, Saki Tomiyama, Atsuko Isogai, Tetsuya Goshima, Farzan Ghanegolmohammadi, Tomoyuki Nishi, Katsuhiko Kitamoto, Takeshi Akao, Dai Hirata, Yoshikazu Ohya
Summary: Modifying the genetic background and introducing targeted mutations are essential for breeding desired traits in crops, livestock, and microorganisms. However, it is not well understood how similar traits emerge when the same mutation is introduced into different genetic backgrounds.
Article
Biochemistry & Molecular Biology
Weronika Rzepnikowska, Joanna Kaminska, Andrzej Kochanski
Summary: In this study, a simple yeast model was developed to evaluate the significance of IGHMBP2 gene mutations in the development of SMARD1. The results showed that the model can distinguish between pathogenic and non-pathogenic mutations, providing important insights for clinical diagnosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Arya Vindu, Byung-Sik Shin, Kevin Choi, Eric T. Christenson, Ivaylo P. Ivanov, Chune Cao, Anirban Banerjee, Thomas E. Dever
Summary: Polyamines are essential for cell viability and their levels are maintained by post-transcriptional regulation of key biosynthetic enzymes. This study shows that the membrane transporter Hol1 in S. cerevisiae is under translational control by polyamines and is required for yeast growth under limiting polyamine conditions. Additionally, polyamines inhibit the translation factor eIF5A to repress Hol1 synthesis under conditions of elevated polyamines, highlighting the extensive control cells impose on polyamine levels.
Article
Biochemistry & Molecular Biology
Kunal Chatterjee, William A. Marshall, Anita K. Hopper
Summary: This study identifies a new tRNA nuclear exporter and reveals exporter preferences for specific tRNA families. Additionally, it demonstrates the importance of tRNA nuclear import in tRNA quality control.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Jun-Min Lee, Min-Ho Park, Bu-Soo Park, Min-Kyu Oh
Summary: This study achieved microbial production of S-methyl-methionine (SMM) by introducing a plant-derived MMT gene into engineered Saccharomyces cerevisiae sake K6.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Tyler W. Doughty, Rosemary Yu, Lucy Fang- Chao, Zhongjun Qin, Verena Siewers, Jens Nielsen
Summary: The single chromosome strain exhibited poorer growth characteristics on non-fermentable carbon sources due to sirtuin-mediated silencing. Nicotinamide was able to rescue the diminished growth of the strain on ethanol.
Article
Microbiology
Masataka Ohashi, Shota Isogai, Hiroshi Takagi
Summary: This study revealed the crucial role of key residues in the formation of the arginine-binding cavity for arginine recognition of NAGK. Additionally, novel arginine feedback inhibition-insensitive variants of NAGK in sake yeast mutants with ornithine overproduction were analyzed, suggesting that amino acid substitutions in the NAGK variants destabilize the arginine-binding cavity, leading to lower sensitivity to arginine feedback inhibition of NAGK activity.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Lifang Yu, Mario Andrea Marchisio
Summary: This study presents an efficient tool to induce mRNA degradation in yeast using CRISPR-(d)Cas system, specifically (d)Cas12a. The study highlights the potential of V-A Cas proteins in yeast and demonstrates their applications in constructing Boolean logic gates.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Yan Guo, Huiyi Wang, Xin Wei, Zhiqi Wang, Huaiguang Wang, Jing Chen, Jianbin Li, Jidong Liu
Summary: In this study, a novel K+ uptake channel was introduced to the SAM production pathway in Saccharomyces cerevisiae, activated by K+ in molasses to enhance SAM synthase (SAM-s) activity and improve SAM production efficiency. By combining with bio-process optimization, a final yield of 2.22 g/L was achieved from pretreated molasses. This work provides a practical and cost-effective strategy for large-scale biosynthesis of SAM from cane molasses.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Multidisciplinary Sciences
William M. Shaw, Lucie Studena, Kyler Roy, Piotr Hapeta, Nicholas S. McCarty, Alicia E. Graham, Tom Ellis, Rodrigo Ledesma-Amaro
Summary: CRISPR gene activation and inhibition is a powerful synthetic tool for influencing gene expression, with applications in foundational studies, cellular reprogramming, and metabolic engineering. The authors developed a method for inducible expression of multiple gRNAs, increasing the capacity for CRISPR gene activation and inhibition and enhancing flexibility in target gene manipulation. They also created a highly regulated and user-friendly CRISPR toolkit in yeast, enabling simultaneous activation and repression of 11 genes in central metabolism, resulting in a significant increase in succinic acid production.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Mrinal Sharma, Prranshu Yadav, Ankita Doshi, Hemang D. Brahmbhatt, C. Ratna Prabha
Summary: The study examined the effects of six double mutants derived from the ubiquitin mutant UbEP42 on cell functions, finding that the double mutation L50P-I61T had the most detrimental impact. Overall, these double mutations made cells more sensitive, and the negative effects under certain conditions could be compensated for by each other.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Cell Biology
Akos Farkas, Henning Urlaub, Katherine E. Bohnsack, Blanche Schwappach
Summary: The study reveals the important role of the guided entry of tail-anchored proteins (GET) pathway in lipotoxicity and uncovers the hairpin membrane protein Erg1 as a non-canonical GET pathway client. The GET pathway is particularly important for the acute upregulation of Erg1 under low sterol conditions, and several other proteins anchored to the endoplasmic reticulum via a hairpin are also identified as potential GET clients.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Ignacio Belda, Thomas C. Williams, Miguel de Celis, Ian T. Paulsen, Isak S. Pretorius
Summary: Synthetic metagenomics has the potential to reveal the complexity of microbial ecosystems by showing the simplicity of microbial communities captured in a single cell. Conceptually, a yeast cell carrying a representative synthetic metagenome could uncover the complexity of interactions between multiple species, as illustrated here with wine ferments.
NATURE COMMUNICATIONS
(2021)
Article
Water Resources
Mohaddeseh Zahmatkesh Anbarani, Afsaneh Esmaeili Nasrabadi, Ziaeddin Bonyadi
Summary: The aim of this study was to evaluate the efficiency of S. cerevisiae in removing PS from aqueous solutions. BBD was used to determine the optimal removal conditions. The maximum PS removal efficiency was 98.81% under optimized conditions. Based on the results, it can be concluded that S. cerevisiae can be used as a natural and environmentally friendly biocoagulant to remove PS.
APPLIED WATER SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Tetsuya Goshima, Ryo Nakamura, Kazunori Kume, Hiroki Okada, Eri Ichikawa, Hiroyasu Tamura, Hirokazu Hasuda, Masaaki Inahashi, Naoto Okazaki, Takeshi Akao, Hitoshi Shimoi, Masaki Mizunuma, Yoshikazu Ohya, Dai Hirata
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2016)
Article
Multidisciplinary Sciences
Takafumi Ogawa, Ryohei Tsubakiyama, Muneyoshi Kanai, Tetsuya Koyama, Tsutomu Fujii, Haruyuki Iefuji, Tomoyoshi Soga, Kazunori Kume, Tokichi Miyakawa, Dai Hirata, Masaki Mizunuma
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Biotechnology & Applied Microbiology
Muneyoshi Kanai, Tomoko Kawata, Yoshie Yoshida, Yasuko Kita, Takafumi Ogawa, Masaki Mizunuma, Daisuke Watanabe, Hitoshi Shimoi, Akihiro Mizuno, Osamu Yamada, Tsutomu Fujii, Haruyuki Iefuji
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2017)
Article
Biochemistry & Molecular Biology
Kazunori Kume, Sayuri Kaneko, Kenji Nishikawa, Masaki Mizunuma, Dai Hirata
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Article
Genetics & Heredity
Karen Kubo, Hiroki Okada, Takuya Shimamoto, Yoshitaka Kimori, Masaki Mizunuma, Erfei Bi, Shinsuke Ohnuki, Yoshikazu Ohya
Article
Multidisciplinary Sciences
Takahiro Ogawa, Yukihiro Kodera, Dai Hirata, T. Keith Blackwell, Masaki Mizunuma
SCIENTIFIC REPORTS
(2016)
Article
Biochemistry & Molecular Biology
Muneyoshi Kanai, Norito Yasuda, Tomoko Morimoto, Satoko Yoshida, Nahoko Nishibori, Masaki Mizunuma, Tsutomu Fujii, Haruyuki Iefuji
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Koji Masumura, Sachi Matsukami, Kumiko Yonekita, Muneyoshi Kanai, Kazunori Kume, Dai Hirata, Masaki Mizunuma
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Muneyoshi Kanai, Tomoko Kawata, Tomoko Morimoto, Masaki Mizunuma, Daisuke Watanabe, Takeshi Akao, Tsutomu Fujii, Haruyuki Iefuji
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2020)
Article
Cell Biology
Takafumi Ogawa, Koji Masumura, Yuki Kohara, Muneyoshi Kanai, Tomoyoshi Soga, Yoshikazu Ohya, T. Keith Blackwell, Masaki Mizunuma
Summary: Methionine restriction can extend lifespan and delay aging-associated pathologies. Supplementation with S-adenosyl-L-homocysteine (SAH) can mimic the effects of methionine restriction by reducing methionine levels and activating AMP-activated protein kinase (AMPK), thereby extending lifespan.
Article
Microbiology
Tianyu Zhao, Asaka Chida, Yuichi Shichino, Dongwoo Choi, Masaki Mizunuma, Shintaro Iwasaki, Yoshikazu Ohya
Summary: This study reveals the global translational regulation during replicative aging in yeast using ribosome profiling technique. The research found that the translational efficiency of genes involved in DNA repair and chromosome organization increased, while the translational efficiency of genes encoding ribosome components, transposons and cell wall assembly decreased during replicative aging.
Review
Biotechnology & Applied Microbiology
Muneyoshi Kanai, Masaki Mizunuma, Tsutomu Fujii, Haruyuki Iefuji
Summary: Sake yeasts possess specific brewing characteristics that are beneficial for sake making, and they also accumulate various functional components such as S-adenosylmethionine (SAM) and folate. Understanding the mechanisms of these components is crucial for fully characterizing "sake yeast identity" and improving the sake brewing process.
FEMS YEAST RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Kazunori Kume, Tomoyo Hashimoto, Masashi Suzuki, Masaki Mizunuma, Takashi Toda, Dai Hirata
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2017)
Article
Biochemistry & Molecular Biology
Ryo Nakamura, Kumiko Nakano, Hiroyasu Tamura, Masaki Mizunuma, Tohru Fushiki, Dai Hirata
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2017)
Review
Biotechnology & Applied Microbiology
Muneyoshi Kanai, Masaki Mizunuma, Tsutomu Fujii, Haruyuki Iefuji
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2017)