Article
Energy & Fuels
Jeeraporn Pekkoh, Khomsan Ruangrit, Chayakorn Pumas, Kritsana Duangjan, Supakit Chaipoot, Rewat Phongphisutthinant, Itthipon Jeerapan, Kasirawat Sawangrat, Wasu Pathom-aree, Sirasit Srinuanpan
Summary: This study produced protein hydrolysates from microalgal Chlorella biomass using enzymatic hydrolysis, which showed cosmetic and nutraceutical properties. The hydrolysates had high degree of hydrolysis and bioactivities, including antioxidative and tyrosinase inhibition activities. The molecular weight of the hydrolysates was determined to be <= 2 kDa, and they contained essential amino acids meeting the human nutrient requirements. This highlights the potential use of enzymatically derived protein hydrolysates from microalgae in cosmetic and nutraceutical applications.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Biotechnology & Applied Microbiology
Jinzhi Yang, Di Cai, Xudong Liu, Liqi Zhu, Changwei Zhang, Qing Peng, Yanxia Han, Guozhen Liu, Ming Yang
Summary: This study compared the enzymatic hydrolysis efficiency of wet, freeze-dried, and oven-dried microalgal biomass, with and without an acidic pretreatment. The results showed that wet microalgal biomass had a higher glucose yield compared to oven-dried biomass, possibly due to the hindered accessibility of enzymes in the cross-linked cells of the latter. Acidic pretreatment significantly improved the glucose recovery, with potential for direct saccharification process. The enzymatic hydrolysate of wet microalgal biomass was successfully used for ABE fermentation, producing 7.2 g/L of ABE, indicating its potential in bioalcohol fuel fermentation.
FERMENTATION-BASEL
(2023)
Article
Agricultural Engineering
Hong Liu, Zhiping Zhang, Chaoyang Lu, Jian Wang, Kaixin Wang, Siyi Guo, Quanguo Zhang
Summary: The method of enzymatic hydrolysis after alkaline pretreatment significantly improves the production efficiency of biohydrogen from microalgae biomass, providing scientific reference for the development of efficient and low-cost biohydrogen production technology.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Laixin Dai, Melanie Cepeda, Joerg Hinrichs, Jochen Weiss
Summary: The study investigated the emulsifying behavior of untreated insoluble microalgae protein fraction and two of its hydrolysates obtained at different temperatures and acid concentrations. Emulsions with Hydrolysates 85 showed the smallest droplet size and highest apparent viscosity, with gravitational separation hindered as oil content increased. The results suggest that Hydrolysates 85 may be a good emulsifier for concentrated emulsion-based foods with high oil content.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Agricultural Engineering
Yingying Ke, Jun Chen, Taotao Dai, Ruihong Liang, Wei Liu, Chengmei Liu, Lizhen Deng
Summary: To facilitate biomolecules extraction and bioaccessibility of Chlorella pyrenoidosa, a novel industry-scale microfluidization (ISM) was used to disrupt cells effectively. Microscope images showed ISM damaged cell integrity, disorganized cell wall structure, pulverized cell membrane and promoted the release of intracellular components. The decrease of particle size and the increase of ζ-potential also confirmed the cell disruption. The cell breakage ratio of sample treated at 120 MPa was 98%. Compared with untreated samples, total soluble solid content and protein extraction rate of the sample treated at 120 MPa increased by 2 degrees Brix and 12%. Protein was degraded by ISM, the release of intracellular protein and the reduction of molecular weight increased protein digestibility by 20% in in vitro gastric phase. Lipid yield and chlorophyll b content were also increased by ISM. These results provided a new solution to cell disruption of microalgae and expanded the application field of ISM.
BIORESOURCE TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Miguel Angulo, Ma Carmen Marquez
Summary: This research focuses on the enzymatic hydrolysis of meat waste as a green technology to obtain high-value products. Optimized process variables for protein hydrolysis included pH 8.0, temperature 50°C, and Eo/So 0.16 AU/g, resulting in easily digestible and pleasant-tasting protein hydrolysates and collagen. For lipid hydrolysis, the optimal process variables were Eo'/So' 0.83 kLU/g, pH 8.0, and temperature 50°C, with unsaturated fatty acids being the predominant components. The combination of Alcalase and Resinase enzymes showed potential for simultaneous recovery of protein hydrolysates, collagen, and fatty acids.
APPLIED SCIENCES-BASEL
(2023)
Article
Multidisciplinary Sciences
Sophie Weber, Philipp M. Grande, Lars M. Blank, Holger Klose
Summary: With their ability to fix CO2 using sunlight, algae and microalgae are being studied for the production of proteins and valuable compounds. However, the successful utilization of algal biomass relies on effectively disrupting the recalcitrant cell wall. This study evaluated different methods to break up the cell wall and measured success by protein and carbohydrate release. The analysis also identified factors contributing to the robustness of the cell wall.
Review
Food Science & Technology
Yuanhao Chen, Honghao Liang, Hong Du, Valentina Jesumani, Weiling He, Kit-Leong Cheong, Tangcheng Li, Ting Hong
Summary: The world is currently facing hunger issues and new environmentally friendly food sources are needed. Microalgae offer potential as a source of nutrients and bioactive components for high-value products. However, there are problems in the microalgae food industry, such as evaluating nutritional value and limited market data.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Monirul Islam, Yatao Huang, Serajul Islam, Bei Fan, Litao Tong, Fengzhong Wang
Summary: Soybean protein hydrolysates were prepared using Alcalase and Protamex, and their degree of hydrolysis and functional properties were evaluated. Protamex showed excellent solubility, emulsifying activity, and foaming capacity, while Alcalase had high water-holding capacity. Additionally, Protamex exhibited the strongest antioxidant activity. These findings indicate the potential of soybean protein hydrolysates in improving the functional properties, antioxidant activity, and nutritional values of soybeans.
Article
Chemistry, Multidisciplinary
Aline C. C. Pena, Lucas Manique Raymundo, Luciane F. Trierweiler, Mariliz Gutterres
Summary: In this study, microalgae biochar was used to synthesize carbon dots (CDs) for detecting toxic metal ions. The synthesized CDs showed high fluorescence intensity and stability. The results demonstrated selective quenching ability of the CDs towards Cr (VI), indicating their potential for metal ion detection.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Kourosh Ghodrat Jahromi, Zhila Heydari Koochi, Gholamreza Kavoosi, Alireza Shahsavar
Summary: Citrus peel fatty acids can be used as an inexpensive organic nutrient source for the cultivation of Chlorella, leading to increased biomass, lipid production, and nutritional quality.
SCIENTIFIC REPORTS
(2022)
Article
Agricultural Engineering
Gabriela F. Ferreira, Luisa F. Rios Pinto, Rubens Maciel Filho, Leonardo V. Fregolente
Summary: This study aimed to cultivate Chlorella vulgaris and Desmodesmus sp. using sugarcane biorefinery residues bagasse and vinasse, exploring the effects of varying growth conditions. The results showed that under specific conditions, high cell density and specific metabolite accumulation can be achieved.
BIORESOURCE TECHNOLOGY
(2021)
Article
Food Science & Technology
Mohammed S. Qoms, Brisha Arulrajah, Rosnah Shamsudin, Nurul Shazini Ramli, Wan Zunairah Wan Ibadullah, De-Ming Chau, Nazamid Saari
Summary: This study produced Azolla pinnata fern protein concentrate (AFPC) through alkaline solubilisation with isoelectric precipitation and investigated the effects of different proteases on the physicochemical, techno-functional properties, and biological activities of Azolla fern protein hydrolysates. The results showed that enzymolysis with all proteases decreased hydrophobicity, denaturation temperature, and secondary structures as the degree of hydrolysis increased. Techno-functional properties including water and oil holding capacities, emulsion properties, and foam stability were reduced with increasing hydrolysis, except for solubility and foaming capacity. Hydrolysis with all proteases had a positive effect on antioxidant and antibacterial activities. The study suggests the potential application of Azolla fern protein hydrolysates as nutraceutical and functional food ingredients.
Article
Food Science & Technology
Wenhan Zhang, Nan Jia, Zihao Zhu, Yanchao Wang, Jingfeng Wang, Changhu Xue
Summary: This study investigated the effects of enzymatic hydrolysis and activated carbon decoloration on antioxidative activities and peptidomic patterns of microalgae protein hydrolysates using a peptidomic approach. Activated carbon decoloration significantly increased color lightness but decreased antioxidative activities and relative abundances of peptides, particularly aromatic amino acid containing peptides. Understanding the dynamics of production processes at a molecular scale can enhance the production and quality control of bioactive peptides from Auxenochlorella pyrenoidosa.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Energy & Fuels
Amanpreet Kaur, Monica Sachdeva Taggar, Anu Kalia, Manpreet Singh
Summary: Limiting nitrate concentration can promote the accumulation of carbohydrates and starch in microalgae, while optimizing the nitrate concentration is beneficial for the production of high-value carotenoids, contributing to the economic feasibility of bioethanol production.
BIOENERGY RESEARCH
(2022)
Article
Fisheries
Pablo S. Hernandez-Hurtado, Hector Nolasco-Soria, Olimpia Carrillo-Farnes, Helios Hernandez-Hurtado, Rafael Garcia de Quevedo-Machain, Gustavo Casas-Andreu, Cynthia Montoya-Martinez, Fernando Vega-Villasante
LATIN AMERICAN JOURNAL OF AQUATIC RESEARCH
(2018)
Article
Fisheries
Cynthia Montoya-Martinez, Hector Nolasco-Soria, Fernando Vega-Villasante, Olimpia Carrillo-Farnes, Alfonso Alvarez-Gonzalez, Roberto Civera-Cerecedo
LATIN AMERICAN JOURNAL OF AQUATIC RESEARCH
(2018)
Article
Fisheries
Cynthia E. Montoya-Martinez, Cristian D. Carrillo-Perez, Hector Nolasco-Soria, Alfonso Alvarez-Gonzalez, Olimpia Carrillo-Farnes, Roberto Civera-Cerecedo, Fernando Vega-Villasante
LATIN AMERICAN JOURNAL OF AQUATIC RESEARCH
(2018)
Article
Fisheries
Cynthia Montoya-Martinez, Hector Nolasco-Soria, Fernando Vega-Villasante, Olimpia Carrillo-Farnes, Alfonso Alvarez-Gonzalez, Roberto Civera-Cerecedo
LATIN AMERICAN JOURNAL OF AQUATIC RESEARCH
(2018)
Article
Fisheries
Jorge Manuel Lopez-Huerta, Fernando Vega-Villasante, Maria Teresa Viana, Olimpia Carrillo-Farnes, Daniel Badillo-Zapata
LATIN AMERICAN JOURNAL OF AQUATIC RESEARCH
(2018)
Article
Microbiology
Gabriel Llaurado Maury, Humberto J. Morris-Quevedo, Annick Heykers, Ellen Lanckacker, Davie Cappoen, Peter Delputte, Wim Vanden Berghe, Zelene Salgueiro, Paul Cos
Summary: The study found that Pleurotus ostreatus mycelium extract can activate macrophage functions, increase nitric oxide production, elevate levels of TNF-alpha and IL-6, and enhance phagocytic activity in macrophages. It also stimulates the activation of NF-kappa B promoter.
Article
Microbiology
Georgina Uriarte-Frias, Martha M. Hernandez-Ortega, Gabriela Gutierrez-Salmean, Miriam Magale Santiago-Ortiz, Humberto J. Morris-Quevedo, Marcos Meneses-Mayo
Summary: This study evaluated the impact of partial replacement of whole-wheat flour with oyster mushroom, nopal, and amaranth flours on the nutritional and antioxidant properties of fortified cookies. The fortified cookies showed higher protein, ash, flavonoids, dietary fiber, and polyphenol content, as well as improved scavenging ability of ABTS(& BULL;+) compared to traditional cookies. The study demonstrates the potential of oyster mushroom, nopal, and amaranth flours as sustainable ingredients for functional cookie production.
Article
Pharmacology & Pharmacy
Clara A. Berenguer-Rivas, Onel Fong Lores, Julio C. Escalona-Arranz, Jorge de la Vega-Acosta, Diana J. Arro-Diaz, Frenkel Guisado-Bourzac, Gabriel Llaurado-Maury, Humberto J. Morris-Quevedo
Summary: The study evaluated the cytoprotective activity of Adelia ricinella extracts on red blood cells membrane, showing significant protection against oxidative damage-induced hematological alterations. The extracts also exhibited no cytotoxicity on mammalian Vero cells.
JOURNAL OF PHARMACY & PHARMACOGNOSY RESEARCH
(2021)
Article
Plant Sciences
Yaixa Beltran Delgado, Humberto Morris Quevedo, Daliannis Oliva Dominguez, Pedro Batista Corbal, Gabriel Llaurado Maury
Summary: The study found that the content of proteins, reducing sugars, and flavonoids was higher in the aqueous extract of mature fruiting bodies of Pleurotus ostreatus, while carbohydrates and total phenols were increased in the extract of primordia. The antioxidant assays showed that the EC50 values were lower in the extract of mature fruiting bodies compared to the extract of primordia.
ACTA BIOLOGICA COLOMBIANA
(2021)
Article
Pharmacology & Pharmacy
Rodolfo Rodriguez-Ravelo, Ariel Ruiz-Urquiola, Lourival D. Possani-Postay, Humberto J. Morris-Quevedo, Magdelaine Rodriguez-Ravelo, Georgina Espinosa-Lopez
Summary: In this study, populations of R. junceus from different districts in Moanicum sector of Cuba were characterized using genetic and proteomic analysis, revealing significant genetic differentiation and variation in venom composition among districts. The findings suggest a diverse scorpion population with different toxin profiles, which could have implications for pharmacological applications.
JOURNAL OF PHARMACY & PHARMACOGNOSY RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Gabriel Llaurado Maury, Daniel Mendez Rodriguez, Sophie Hendrix, Julio Cesar Escalona Arranz, Yilan Fung Boix, Ania Ochoa Pacheco, Jesus Garcia Diaz, Humberto J. Morris-Quevedo, Albys Ferrer Dubois, Elizabeth Isaac Aleman, Natalie Beenaerts, Isidro E. Mendez-Santos, Teresa Orbera Raton, Paul Cos, Ann Cuypers
Article
Medicine, Research & Experimental
Cristina Rey-Blanes, Yalina Perez-Portero, Humberto J. Morris-Quevedo, Virginia Casas, Roberto Abdala, Ana R. Quesada, Beatriz Martinez-Poveda, Miguel Angel Medina
BIOMEDICINE & PHARMACOTHERAPY
(2020)
Article
Pharmacology & Pharmacy
Gabriel Llaurado, Yaixa Beltran, Humberto J. Morris, Ebert Marcos, Usnavia Diaz, Jane Marcos, Jesus Garcia, Dunilka Disotuar, Paul Cos
JOURNAL OF PHARMACY & PHARMACOGNOSY RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Yamila Lebeque, Humberto J. Morris, Yaixa Beltran, Gabriel Llaurado, Isabelle Gaime-Perraud, Marcos Meneses, Serge Moukha, Rosa C. Bermudez, Nora Garcia
INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS
(2018)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)