Article
Multidisciplinary Sciences
Jaimie M. Mayner, Evan M. Masutani, Elena Demeester, Aditya Kumar, Gail Macapugay, Pranjali Beri, Valentina Lo Sardo, Adam J. Engler
Summary: 9p21.3 locus polymorphisms show the strongest correlation with coronary artery disease, but the connection is unclear due to its noncoding nature. The lncRNA ANRIL in 9p21.3 may regulate VSMC phenotype to contribute to disease risk. Different subpopulations of risk haplotype cells exhibit variable morphology, proliferation, contraction, and adhesion, suggesting that variable lncRNA penetrance may drive mixed functional outcomes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Akihito Kira, Ichiko Tatsutomi, Keisuke Saito, Machiko Murata, Izumi Hattori, Haruna Kajita, Naoko Muraki, Yukako Oda, Saya Satoh, Yuta Tsukamoto, Seisuke Kimura, Kenta Onoue, Shigenobu Yonemura, Satoko Arakawa, Hiroki Kato, Tsuyoshi Hirashima, Kohki Kawane
Summary: Cell extrusion is a universal mechanism for cell removal from tissues, and its understanding is crucial for maintaining tissue homeostasis. This study reveals a conserved execution mechanism of apoptotic cell extrusion, where extracellular vesicles (EVs) play a key role. EV formation is regulated by lipid-scramblase-mediated phosphatidylserine exposure and promotes neighboring cells' invasion. This research highlights the importance of membrane dynamics in coordinating cell exit and demonstrates the significance of EV formation in cell delamination and tissue homeostasis.
DEVELOPMENTAL CELL
(2023)
Article
Physics, Multidisciplinary
Xuechen Shi, Zezhou Liu, Luyi Feng, Tiankai Zhao, Chung-Yuen Hui, Sulin Zhang
Summary: The study investigates the elastocapillary effect at cell-matrix contacts and its impact on tissue morphogenesis, wound healing, and cancer metastasis. By observing cell colonies indenting soft hydrogels, the researchers propose a two-step traction force microscopy to separate active cell traction from interfacial stresses. The study provides a foundation for understanding elastocapillarity-induced mechanobiological processes in cells and tissues.
Article
Multidisciplinary Sciences
Robert S. Fischer, Xiaoyu Sun, Michelle A. Baird, Matt J. Hourwitz, Bo Ri Seo, Ana M. Pasapera, Shalin B. Mehta, Wolfgang Losert, Claudia Fischbach, John T. Fourkas, Clare M. Waterman
Summary: This study investigated the impact of contact guidance on cell migration, showing that high-amplitude fiber wave substrates inhibited cell polarization and directional migration, while low-amplitude substrates promoted directional migration. Manipulating the amplitude of ECM fibril waveforms can affect the polarization response of cancer cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Courtney M. Klappenbach, Nicholas M. Negretti, Jesse Aaron, Teng-Leong Chew, Michael E. Konkel
Summary: Our study reveals that C. jejuni infection alters the size and topology of focal adhesions in epithelial cells, leading to changes in cell adhesion strength and motility. The increased phosphorylation of paxillin and altered turnover at focal adhesions may contribute to the effects on cell behavior. Additionally, inhibiting protein synthesis or using specific mutants of C. jejuni can partially reverse these effects, suggesting a potential mechanism for the observed restricted intestinal repair in infected animals.
Review
Developmental Biology
Naoya Yamaguchi, Holger Knaut
Summary: Cell-extracellular matrix interactions are essential for cell anchoring and migration, with focal adhesions (FAs) playing a key role. However, the role of FAs in vivo is still not well understood.
Article
Oncology
Jiyuan Hu, Linhui Wang, Luanfeng Li, Yutao Wang, Jianbin Bi
Summary: A prognostic model consisting of 7 genes was constructed using gene analysis and bioinformatics methods, which can predict the survival risk and overall survival of bladder cancer patients effectively.
Article
Multidisciplinary Sciences
Jui-Chien Lien, Yu-li Wang
Summary: The reorientation of adherent cells upon uniaxial cyclic stretching is primarily driven by relaxation phase, during which rapid global retraction and extension transverse to the direction of stretching take place. Myosin II inhibition causes cells to orient along the direction of stretching, while disassembly of microtubules enhances transverse reorientation. This study highlights the distinct roles of stretching and relaxation in cell reorientation.
SCIENTIFIC REPORTS
(2021)
Article
Biophysics
Shannon M. Flanary, Victor H. Barocas
Summary: This study presents a computational model that captures the major aspects of cell traction process. The model incorporates four interacting components: a biochemical signaling network, individual actomyosin fiber bundle contraction, a cytoskeletal network of interconnected fibers, and elastic substrate displacement due to cytoskeletal force. It provides a framework to interpret TFM data and link biochemical and biomechanical phenomena at the single-cell level.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2023)
Article
Energy & Fuels
Nur Hazlin Hazrin-Chong, Theerthankar Das, Michael Manefield
Summary: This study investigated the influence of surface physico-chemistry on microbial cell attachment on coal, revealing that natural lignite and subbituminous coals offered the most conducive surface for cell attachment. Coal pretreatments significantly affected the interaction between cells and coal, with peroxide treatment showing favorable coal-cell interaction. Additionally, cell colonisation was strongest on lignite due to its relatively high van der Waals surface energy component.
INTERNATIONAL JOURNAL OF COAL GEOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Hiroki Sakuta, Naoki Nakatani, Takayuki Torisawa, Yutaka Sumino, Kanta Tsumoto, Kazuhiro Oiwa, Kenichi Yoshikawa
Summary: Water phase separation in cells can lead to mechanical motion by entrapping protein motors and generating vortex flow inside cell-sized droplets. This study reveals that the water phase separation interface can drive translational motion of droplets by spontaneously entrapping kinesins and microtubules and generating vortex flow. The findings highlight the importance of water phase separation in both chemical and mechanical processes.
COMMUNICATIONS CHEMISTRY
(2023)
Article
Mechanics
Jianlin Huang, Jingzhu Wang, Jian Huang, Pengyu Lv, Hongyuan Li, Yiwei Wang
Summary: This numerical study investigates the influence of wall wettability on the vortex flow and wall shear stress induced by the collapse of a cavitation bubble. The results show that superhydrophobic and hydrophilic surfaces broaden the wall-vortex regimes compared to a neutral surface. The main area of shear stress is enlarged for a superhydrophobic surface and extended spatially for a hydrophilic surface. The findings provide valuable guidance for ultrasonic cleaning in engineering applications.
Article
Cell Biology
Aysegul Dede Eren, Amy W. A. Lucassen, Urandelger Tuvshindorj, Roman Truckenmuller, Stefan Giselbrecht, E. Deniz Eren, Mehmet Orhan Tas, Phanikrishna Sudarsanam, Jan de Boer
Summary: This study investigated how cells organize their adhesive machinery on surfaces with different topographies and adhesive island geometries, and examined the reciprocal interaction between stress fiber and focal adhesion formation. The results confirm that the spatial organization of adhesive sites determines the ability of cells to form focal adhesions and stress fibers.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Gregory Adams, Magdalena Preciado Lopez, Alexander X. Cartagena-Rivera, Clare M. Waterman
Summary: The study revealed the plasticity of migration modes in cancer cells migrating in confined microenvironments, particularly showing leader bleb-based migration in nonadhesive environments. Fluorescently tagged organelle-specific markers and actin-associated proteins localization demonstrated the crucial role of specific actin crosslinkers in LBBM.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Article
Multidisciplinary Sciences
Yuh-Ru Julie Lee, Soichiro Yamada, Su Hao Lo
Summary: Biomolecular condensates formed by liquid-liquid phase separation include GFP-tagged tensin-1 (TNS1) proteins. Live-cell imaging revealed that TNS1 condensates emerge from the disassembling ends of focal adhesions (FAs) and their presence is cell cycle dependent. TNS1 condensates dissolve prior to mitosis and reappear when postmitotic daughter cells form new FAs, containing selected FA proteins and signaling molecules.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Mechanics
L. Molari, M. Maraldi, G. Molari
MECHANICS RESEARCH COMMUNICATIONS
(2017)
Article
Agricultural Engineering
Mirko Maraldi, Luisa Molari, Giovanni Molari, Nicolo Regazzi
BIOSYSTEMS ENGINEERING
(2018)
Article
Endocrinology & Metabolism
N. Movilla, C. Borau, C. Valero, J. M. Garcia-Aznar
Article
Multidisciplinary Sciences
Clara Valero, Hippolyte Amaveda, Mario Mora, Jose Manuel Garcia-Aznar
Article
Engineering, Chemical
A. Chiminelli, C. Valero, M. Lizaranzu, C. I. Lopez, M. Canales
JOURNAL OF ADHESION
(2019)
Article
Agricultural Engineering
Michele Mattetti, Mirko Maraldi, Enrico Sedoni, Giovanni Molari
BIOSYSTEMS ENGINEERING
(2019)
Article
Agricultural Engineering
Michele Mattetti, Stefano Davoli, Mirko Maraldi, Francesco Paolini, Stefano Fiorati, Giovanni Molari
BIOSYSTEMS ENGINEERING
(2019)
Article
Agricultural Engineering
Nicola Regazzi, Mirko Maraldi, Giovanni Molari
BIOSYSTEMS ENGINEERING
(2019)
Article
Agronomy
Massimiliano Varani, Michele Mattetti, Mirko Maraldi, Giovanni Molari
Article
Agriculture, Multidisciplinary
Michele Mattetti, Mirko Maraldi, Nicola Lenzini, Stefano Fiorati, Eugenio Sereni, Giovanni Molari
Summary: Tractor manufacturers can optimize machine design by understanding how farmers utilize their agricultural tractors, which is made easier with sensors integrated into the CAN-BUS network and accessed through the ISO 11783 protocol. A new classification scheme for CAN-BUS data has been developed in this study, allowing for a more precise outline of tractor usage and potential benefits from the collected data.
COMPUTERS AND ELECTRONICS IN AGRICULTURE
(2021)
Article
Agronomy
Lianet Avello Fernandez, Mirko Maraldi, Michele Mattetti, Massimiliano Varani
Summary: This study develops a computational tool for determining an optimized three-point hitch geometry to minimize the weight-transfer effect. Simulation results show that the optimized geometry can effectively reduce the weight-transfer effect.
Article
Construction & Building Technology
Silvia Greco, Mirko Maraldi, Luisa Molari
Summary: This paper proposes a strength-based grading method for bamboo, filling the current lack of grading methods using a four-point bending test. Experimental results show that statistical groups can be defined based on mechanical properties, supporting the appropriateness of strength classes. The study focuses on six different bamboo species grown in Italy, with limited data available in the literature.
CONSTRUCTION AND BUILDING MATERIALS
(2023)
Article
Agricultural Engineering
Michele Mattetti, Massimiliano Varani, Mirko Maraldi, Francesco Paolini, Stefano Fiorati, Giovanni Molari
JOURNAL OF AGRICULTURAL ENGINEERING
(2020)
Article
Cell Biology
Nieves Movilla, Clara Valero, Carlos Borau, Jose Manuel Garcia-Aznar
INTEGRATIVE BIOLOGY
(2019)
Article
Materials Science, Multidisciplinary
Carlos Saenz Ezquerro, Manuel Laspalas, Agustin Chiminelli, Francisco Serrano, Clara Valero