Article
Psychiatry
Anil Srivastava, Oluwagbenga Dada, Jessica Qian, Nzaar Al-Chalabi, Ali Bani Fatemi, Philip Gerretsen, Ariel Graff, Vincenzo De Luca
Summary: Schizophrenia is a chronic psychotic disorder that significantly impacts behavior, thought, and cognition, with possible contributions from both genetic and epigenetic factors. Current research focuses on DNA methylation, histone modification, and non-coding RNA, offering potential new avenues for diagnosis and treatment of schizophrenia. Limitations in current knowledge and potential future research directions are also discussed.
PSYCHIATRY RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Futaba Inoue, Kenbun Sone, Yusuke Toyohara, Yu Takahashi, Asako Kukita, Aki Hara, Ayumi Taguchi, Michihiro Tanikawa, Tetsushi Tsuruga, Yutaka Osuga
Summary: Epigenetic changes play a crucial role in endometrial cancer, including DNA methylation and histone modification. There are currently inhibitors targeting epigenetic regulators in preclinical studies and clinical trials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Jiaxin Liu, Jia-nan Li, Hongyu Wu, Panpan Liu
Summary: Epigenetic regulation plays a crucial role in lymphoma development and is closely associated with various malignancies. Understanding these epigenetic mechanisms can guide the development of clinical treatments.
FRONTIERS IN ONCOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Farzin Zobdeh, Ivan I. Eremenko, Mikail A. Akan, Vadim V. Tarasov, Vladimir N. Chubarev, Helgi B. Schioeth, Jessica Mwinyi
Summary: Migraine is a complex neurological disorder and current drug treatments have limited success rates. Understanding the epigenetic changes associated with migraine may help to improve treatment strategies and identify new therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Yuhao Zhao, Mao Yang, Shijia Wang, Sk Jahir Abbas, Junzhe Zhang, Yongsheng Li, Rong Shao, Yingbin Liu
Summary: This review focuses on the mechanistic insights of DNA, histone, and RNA methylation in regulating the progression of pancreatic cancer. The roles of methylation regulators in modulating gene expression associated with cell proliferation, invasion, and apoptosis are discussed. Recent clinical trials on methylation drug targeting are also explored. Understanding the novel regulatory mechanisms of methylation modification may offer alternative opportunities to improve therapeutic efficacy in combating this devastating disease.
FRONTIERS IN ONCOLOGY
(2022)
Review
Oncology
Francisco Gimeno-Valiente, Gerardo Lopez-Rodas, Josefa Castillo, Luis Franco
Summary: This review focuses on the interconnections between epigenetics and alternative splicing in the development of cancer. It discusses the mechanisms involved in these interconnections and the potential diagnostic and therapeutic tools that can be derived from them. The reversible nature of epigenetic alterations and the possibility of correcting aberrant alternative splicing offer promising therapeutic possibilities for cancer treatment.
Article
Biochemistry & Molecular Biology
Deimante Zukauskaite, Aida Vitkeviciene, Akvile Zlibinaite, Raminta Bausyte, Diana Ramasauskaite, Ruta Navakauskiene
Summary: The aim of this study was to analyze the changes in epigenetic modulators, histone modifications, and DNA methylation during induced human endometrial stromal cells (ESC) in vitro decidualization. The results revealed that HDAC2 and subunits of PRC2 were down-regulated while the global level of H4hyperAc and H3K27me3 increased during ESC decidualization. Specific gene promoters' histone acetylation level also increased during the induced ESC decidualization, indicating the importance of epigenetic regulation in endometrial decidualization.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alison C. Brewer
Summary: The epigenetic landscape plays a crucial role in gene transcription and cellular phenotype determination, with chromatin remodelling during cellular differentiation being a key factor. Redox-dependent molecular mechanisms, including NADPH oxidases generating ROS, are important in regulating epigenetic changes. The role of NADPH oxidases in redox-dependent chromatin remodelling and the impact of epigenetic changes on ROS-producing enzymes are discussed in relation to cellular differentiation and homeostasis, particularly focusing on Nox4.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Review
Chemistry, Medicinal
Carmen Nunez-Carro, Margarita Blanco-Blanco, Karla Mariuxi Villagran-Andrade, Francisco J. Blanco, Maria C. de Andres
Summary: In this study, we reviewed the current understanding of epigenetic mechanisms in relation to the pathogenesis of OA, particularly in articular cartilage. Evidence suggests that dysregulation of crucial cartilage molecules is a result of aberrant epigenetic regulatory mechanisms, which contribute to the development and progression of OA. This provides an opportunity to explore new therapeutic targets and novel biomarkers for the disease.
Review
Biochemistry & Molecular Biology
Disha Agarwal, Ruchika Kumari, Ashal Ilyas, Shweta Tyagi, Rajnish Kumar, Nitesh Kumar Poddar
Summary: Epigenetics has opened a new dimension in exploring unanswered questions in genetics, particularly in neurodegenerative diseases like Alzheimer's and cancers. The alterations in DNA methylation levels, histone acetylation, and methylation play a significant role in conditions such as Alzheimer's disease. Studying the interplay between the mTOR regulatory pathway and epigenetic machinery may lead to early diagnosis and novel therapeutic strategies for Alzheimer's disease.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Genetics & Heredity
Merrick S. Fallah, Dora Szarics, Clara M. Robson, James H. Eubanks
Summary: Epigenetic processes play a critical role in regulating gene expression patterns in neurodevelopment, including the dynamic network of post-translational histone modifications. Mutations in histone-modifying enzymes can disrupt this system and lead to developmental consequences. This review examines neurodevelopmental conditions associated with histone methylation and acetylation, discussing clinical phenotypes, genetic bases, and model systems for studying pathological mechanisms.
FRONTIERS IN GENETICS
(2021)
Review
Biochemistry & Molecular Biology
Rachel J. Kehrberg, Namita Bhyravbhatla, Surinder K. Batra, Sushil Kumar
Summary: Cancer-associated fibroblasts (CAFs), a significant component of the tumor microenvironment (TME), contribute to cancer progression through the secretion of extracellular matrix (ECM), growth factors, and metabolites. It is now well recognized that CAFs are a heterogenous population with ablation experiments leading to reduced tumor growth and single-cell RNA sequencing demonstrating CAF subgroups. CAFs lack genetic mutations yet substantially differ from their normal stromal precursors.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2023)
Review
Pharmacology & Pharmacy
Chaithanya Ganji, Batoul Farran
Summary: This review focuses on dysregulated genes associated with epigenetic mechanisms in pancreatic cancer progression and resistance, as well as current clinical trials for epigenetic drugs. Combining epigenetic drugs with targeted therapies might represent a promising approach for treating pancreatic cancer.
DRUG DISCOVERY TODAY
(2022)
Review
Genetics & Heredity
Subramaniam Jayanthi, Michael T. McCoy, Jean Lud Cadet
Summary: Methamphetamine-use disorder is a serious condition that can lead to long-term changes in the brain and gene expression, highlighting the importance of researching the underlying mechanisms.
Review
Oncology
Richard Sean Lee, Kirti Sad, Dorelle V. Fawwal, Jennifer Marie Spangle
Summary: Breast cancer is a frequent and deadly disease in women, with histone-modifying enzymes playing a role in treatment response and patient outcomes. Epigenetic changes to chromatin affect the tumor environment and can be targeted for breast cancer treatment. Understanding these mechanisms may improve therapeutic interventions and patient outcomes.
Article
Biochemistry & Molecular Biology
Reynand Jay Canoy, Franck Andre, Anna Shmakova, Joelle Wiels, Marc Lipinski, Yegor Vassetzky, Diego Germini
Summary: This article presents a novel and efficient protocol for transfection of B-cell lines and B cells from PBMCs, which are notoriously difficult to transfect. The protocol does not require expensive equipment or reagents and achieves high transfection and viability rates through optimization of salt concentration in the transfection medium and plasmid amount. The validation experiment of generating a TP53(-/-) RPMI8866 lymphoblastoid cell line demonstrates the potential applicability of this method in hematological and blood cancer studies.
Article
Cell Biology
Ekaterina Kiseleva, Olesya Serbina, Anna Karpukhina, Vincent Mouly, Yegor S. Vassetzky
Summary: Facioscapulohumeral muscular dystrophy (FSHD) is a genetic disease caused by the ectopic expression of the DUX4 gene in skeletal muscle, leading to muscle degeneration and replacement by fat and connective tissue. This study demonstrates that the interaction between FSHD myoblasts and MSCs plays a crucial role in the pathophysiology of FSHD, including stimulation of MSC migration, proliferation, and collagen secretion.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Vlada V. Zakharova, Mikhail D. Magnitov, Laurence Del Maestro, Sergey Ulianov, Alexandros Glentis, Burhan Uyanik, Alice Williart, Anna Karpukhina, Oleg Demidov, Veronique Joliot, Yegor S. Vassetzky, Rene-Marc Mege, Matthieu Piel, Sergey Razin, Slimane Ait-Si-Ali
Summary: Imbalance in the system of chromatin-modifying enzymes is a hallmark of diseases such as cancer, as it affects the epigenome and transcriptional reprogramming. This study demonstrates that a loss-of-function mutation in the histone lysine methyltransferase SETDB1 leads to changes in the overall structure and mechanical properties of the nucleus in lung cancer cells, due to genome-wide redistribution of heterochromatin. This perturbs the spatial compartmentalization of chromatin and results in the reversed oncogenic potential of lung adenocarcinoma cells.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Cell Biology
Hong-Toan Lai, Reynand Jay Canoy, Michelangelo Campanella, Yegor Vassetzky, Catherine Brenner
Summary: Hepatocellular carcinoma (HCC), a type of liver cancer, has a poor prognosis and its molecular etiology is still unclear. Abnormalities in genes coding for proteins involved in calcium homeostasis are associated with tumorigenesis, metastasis, and evasion of cell death in HCC.
Review
Developmental Biology
A. A. Shmakova, O. P. Shmakova, A. A. Karpukhina, Y. S. Vassetzky
Summary: The discovery of the CRISPR/Cas system has revolutionized biology and biomedicine in the 21st century. This article discusses the milestones in the development of CRISPR/Cas genome editing technology, from its discovery to current advancements, including medical applications. It also explores the technical and ethical issues associated with editing human embryonic genomes using CRISPR/Cas.
RUSSIAN JOURNAL OF DEVELOPMENTAL BIOLOGY
(2022)
Review
Oncology
Reynand Jay Canoy, Anna Shmakova, Anna Karpukhina, Mikhail Shepelev, Diego Germini, Yegor Vassetzky
Summary: Chromosomal translocations are products of the illegitimate repair of DNA double-strand breaks, leading to the illegitimate joining of non-homologous chromosomal ends. They are associated with various diseases. The formation of chromosomal translocations is not only influenced by the proximity of gene loci, but also regulated by other cellular factors.
Letter
Oncology
Anna Shmakova, Nikolai Lomov, Vladimir Viushkov, Tatyana Tsfasman, Yana Kozhevnikova, Darina Sokolova, Vadim Pokrovsky, Marina Syrkina, Diego Germini, Mikhail Rubtsov, Yegor Vassetzky
CANCER COMMUNICATIONS
(2023)
Editorial Material
Oncology
Anna Shmakova, Yegor Vassetzky
INTERNATIONAL JOURNAL OF CANCER
(2023)
Review
Cell Biology
Vladimir S. Viushkov, Nikolai A. Lomov, Mikhail A. Rubtsov, Yegor S. Vassetzky
Summary: The understanding of the genome has evolved from a linear molecule to a complex 3D structure with hierarchical organization. Genomics is now focusing on the study of chromatin dynamics over time. Different methods, such as fluorescent protein targeting and programmed protein targeting, can be used to visualize the movements of chromatin loci. This review discusses and analyzes these methods.
Article
Biochemistry & Molecular Biology
Daniil A. A. Chernyavskij, Olga Yu. Pletjushkina, Anastasia V. V. Kashtanova, Ivan I. I. Galkin, Anna Karpukhina, Boris V. V. Chernyak, Yegor S. S. Vassetzky, Ekaterina N. N. Popova
Summary: Many muscular pathologies involving oxidative stress and elevated TNF levels cause muscle protein catabolism and impair myogenesis. This study found that mitoROS play a role in this process by triggering and enhancing mitophagy during myogenesis. Treatment with TNF before myogenesis decreases myoblast fusion and MYH2 synthesis, but antioxidants and autophagy suppression can partially restore myogenesis.
Review
Cell Biology
Olga Klaudia Szewczyk-Roszczenko, Piotr Roszczenko, Anna Shmakova, Nataliya Finiuk, Serhii Holota, Roman Lesyk, Anna Bielawska, Yegor Vassetzky, Krzysztof Bielawski
Summary: Endocytosis is a major cellular communication process that can be hijacked by pathogens and involved in oncogenic transformation. This review explores different approaches to inhibit endocytosis, discusses chemical inhibitors, and explores potential clinical applications.
Article
Biochemistry & Molecular Biology
Reynand Jay Canoy, Anna Shmakova, Anna Karpukhina, Nikolai Lomov, Eugenia Tiukacheva, Yana Kozhevnikova, Franck Andre, Diego Germini, Yegor Vassetzky
Summary: Most cancer-related chromosomal translocations appear to be specific to certain cell types. However, our study shows that any translocation can potentially arise in any type of cell, and the frequency of translocation is correlated with the spatial proximity between gene loci after double-strand break induction. Furthermore, only specific translocations persisted after long-term culture.
Meeting Abstract
Cell & Tissue Engineering
O. Serbina, E. Kiseleva, Y. Vassetzky
Meeting Abstract
Cell & Tissue Engineering
O. Serbina, E. Kiseleva, A. Karpukhina, Y. Vassetzky
Article
Virology
Margarita A. Kurnaeva, Arthur O. Zalevsky, Eugene A. Arifulin, Olga M. Lisitsyna, Anna Tvorogova, Maria Y. Shubina, Gleb P. Bourenkov, Maria A. Tikhomirova, Daria M. Potashnikova, Anastasia Kachalova, Yana R. Musinova, Andrey Golovin, Yegor S. Vassetzky, Eugene Sheval
Summary: This study investigated the integration of nuclear localization signal (NLS) and nucleolar localization signal (NoLS) into the basic domain of HIV-1 Tat and found that these two supplementary functions are embedded within the amino acid sequence. Integration of NLSs and NoLSs into functional domains of viral proteins enriched with positively charged amino acids allows the concentration of different functions within small protein regions and may have influenced viral evolution by preventing an increase in protein size.
JOURNAL OF VIROLOGY
(2022)
