Article
Chemistry, Multidisciplinary
Niklas Freund, Alexander Taylor, Sebastian Arangundy-Franklin, Nithya Subramanian, Sew-Yeu Peak-Chew, Amy M. Whitaker, Bret D. Freudenthal, Mikhail Abramov, Piet Herdewijn, Philipp Holliger
Summary: In this study, a two-residue "gate" was discovered in an archaeal DNA polymerase, which enables the synthesis of 2'-modified RNA oligomers. This discovery expands the application potential of 2'-modified RNA in nucleic acid therapeutics and biotechnology.
Article
Chemistry, Multidisciplinary
Yuqing Ke, Behafarid Ghalandari, Shiyi Huang, Sijie Li, Chengjie Huang, Xiao Zhi, Daxiang Cui, Xianting Ding
Summary: In this study, we propose a strategy to enhance the specificity of the Cas12a system by using 2'-O-methyl modified guide RNA (gRNA), and we validate the effectiveness of this strategy through experiments. Our results show that the 2'-OMe modified gRNAs can significantly improve the specificity of Cas12a in discriminating single-base mutations, and molecular docking simulations reveal the mechanism of this enhancement.
Article
Microbiology
Ashley M. Edwards, Katherine A. Black, Patricia C. Dos Santos
Summary: This study reveals that in the model Gram-positive organism Bacillus subtilis, the levels of s(2)U are responsive to sulfur availability. Downregulation of the s(2)U biosynthetic components leads to lower s(2)U levels, which may serve as a signal for the slowing of the translational apparatus during cellular nutrient insufficiency.
JOURNAL OF BACTERIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Muthusamy Ramakrishnan, K. Shanmugha Rajan, Sileesh Mullasseri, Sarin Palakkal, Krishnan Kalpana, Anket Sharma, Mingbing Zhou, Kunnummal Kurungara Vinod, Subbiah Ramasamy, Qiang Wei
Summary: There is growing evidence that post-transcriptional RNA modifications can greatly impact crop production. However, the roles of the abundant RNA modifications of pseudouridine and 2'-O-methylation in plants remain unclear. This review discusses the latest methods and insights gained in mapping these modifications in plants, as well as the limitations in current high-throughput technologies. Future challenges in regulating the plant epitranscriptome are also highlighted.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Materials Science, Multidisciplinary
J. Matthew Kurleya, Rodney D. Hunt, Jake W. McMurray, Andrew T. Nelson
Summary: This study synthesized uranium-bearing gel microspheres with a narrow size distribution using microfluidics, and successfully converted them to U 3 O 8 and UO 2 . The experimental results showed that the prepared gel microspheres had a relatively stable broth, fast gelation speed, and small size.
JOURNAL OF NUCLEAR MATERIALS
(2022)
Article
Chemistry, Physical
Basak Kosar Kirca, Cigdem Albayrak Kastas, Cem Cuneyt Ersanli
Summary: Two new Schiff base compounds were synthesized and characterized using various techniques, with density functional theory calculations confirming the most stable configuration as the OH tautomeric form. The crystal structures exhibit significant interactions that play a crucial role in building a three-dimensional network.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Chemistry, Medicinal
Fangjie Lyu, Seongjin An, Yoshiaki Kobayashi, Kohei Nomura, Rintaro Baba, Naoko Abe, Haruka Hiraoka, Fumitaka Hashiya, Zhaoma Shu, Kumiko Ui-Tei, Yasuaki Kimura, Hiroshi Abe
Summary: The medicinal applications of siRNAs have limitations in terms of molecular stability and off-target effects. Chemical modifications to the nucleoside, such as introducing a new uridine analog (U*), can improve the stability of siRNAs under biological conditions and have positive effects on target gene silencing. The study provides useful insights for the development of nucleoside analogs to enhance the molecular properties of siRNAs for medicinal applications.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Roxane Valentin, Christophe Wong, Arwaf S. Alharbi, Solene Pradeloux, Makala P. Morros, Kim A. Lennox, Julia Ellyard, Aurelie J. Garcin, Tomalika R. Ullah, Gina D. Kusuma, Genevieve Pepin, Hong-Mei Li, Jaclyn S. Pearson, Jonathan Ferrand, Rebecca Lim, Rakesh N. Veedu, Eric F. Morand, Carola G. Vinuesa, Mark A. Behlke, Michael P. Gantier
Summary: The study demonstrates that 2'OMe ASOs exhibit sequence-dependent inhibitory effects on RNA sensor TLR7, DNA sensors cGAS and TLR9. Different ASO sequences have varying potency in inhibiting these immune sensors, with only a few shown to inhibit both cGAS and TLR9 pathways. This complex pattern of immunomodulation confounds the interpretation of in vivo functions of ASOs.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Crystallography
Rafal Dolot, Anna Maciaszek, Barbara Mikolajczyk, Barbara Nawrot
Summary: The crystallization experiments of heteroduplexes composed of DNA and 2'-OMe RNA strands showed that overhangs at the ends of the duplex greatly influence the success of crystallization and the crystal packing. The structures of these heteroduplexes, which adopted a conformation close to the A-RNA double helix, provide insight into their properties and can be useful for future applications.
Article
Biochemistry & Molecular Biology
Hajam Towseef Ahmad, K. K. Mohammed Ameen, H. Saleem, M. Syed Ali Padusha, F. M. Mashood Ahamed
Summary: A Schiff base compound was synthesized and characterized using experimental and computational methods. The compound exhibited good antibacterial and antifungal activity.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Chemistry, Physical
M. Hakki Yildirim
Summary: A novel compound, (E)-4,6-dibromo-2-[(2-bromo-4-methylphenylimino)methyl]-3-methoxyphenol, was synthesized and characterized using experimental and computational methods. Its molecular structure, crystal structure, spectral properties, and biological activity were investigated in this study.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Chemistry, Analytical
Miriam Chavez, Guadalupe Sanchez-Obrero, Rafael Madueno, Jose Manuel Sevilla, Manuel Blazquez, Teresa Pineda
Summary: The modification of surfaces by polyethylene glycol is an effective method to reduce antifouling effects in biological media. This study investigated the formation and characteristics of a self-assembled monolayer of EG7-SAM on different surfaces, and found that the EG7-SAM showed the best organization after 1 hour of modification time and exhibited antifouling properties against protein adsorption.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Jennifer Porat, Viktor A. Slat, Stephen D. Rader, Mark A. Bayfield
Summary: Splicing requires tight coordination between dynamic spliceosomal RNAs and proteins. In this study, the authors found that Bmc1 and Pof8 play important roles in the formation of a distinct U6 snRNP that promotes U6 2'-O-methylation, and identified a non-canonical snoRNA guiding this methylation. They also discovered that the 5' γ-monomethyl phosphate capping activity of Bmc1 is not required for its role in promoting snoRNA-guided 2'-O-methylation. These findings provide insights into the novel functions of Bmc1/MePCE family members and the general role of Bmc1 and Pof8 in guiding noncoding RNP assembly.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Energy & Fuels
Noa Miguez, David Fernandez-Polo, Paloma Santos-Moriano, Barbara Rodriguez-Colinas, Ana Poveda, Jesus Jimenez-Barbero, Antonio O. Ballesteros, Francisco J. Plou
Summary: This study presents a simple method for the production of acidic xylooligosaccharides (XOS) from hardwood hemicellulose through enzymatic hydrolysis. The enzyme Depol 670L displayed the highest activity and resulted in high yield of acidic XOS. The acidic XOS exhibited high antioxidant activity.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Chemistry, Multidisciplinary
Virginia Martin-Nieves, Luis Miguel Menendez-Mendez, Carme Fabrega, Susana Fernandez, Yogesh S. Sanghvi, Miguel Ferrero, Ramon Eritja
Summary: Nucleosides and their analogues, especially l-nucleosides, are important molecules with potential antiviral and antiproliferative activity. They have emerged as a new class of therapeutic agents due to their comparable biological activity, improved toxicological properties, and greater metabolic stability compared to d-nucleosides. l-nucleosides can also be used as building blocks for l-oligonucleotides, which have similar physical properties as d-oligonucleotides but are resistant to nuclease degradation, non-toxic, and immunologically passive. The synthesis and incorporation of 2'-O-methyl/2'-O-MOE-l-nucleoside pyrimidine derivatives into G-rich oligonucleotides were described, showing their potential in providing enhanced nuclease resistance without significant impact on nucleic acid structural properties.
Article
Chemistry, Multidisciplinary
Munefumi Tokugawa, Kazuhei Kaneko, Masanori Saito, Takashi Kanamori, Yoshiaki Masaki, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio
Article
Chemistry, Medicinal
Kohji Seio, Yurie Ohno, Kentaro Ohno, Leo Takeshita, Takashi Kanamori, Yoshiaki Masaki, Mitsuo Sekine
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2016)
Article
Chemistry, Medicinal
Yu Ito, Yoshiaki Masaki, Takashi Kanamori, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2016)
Article
Chemistry, Multidisciplinary
Yoshiaki Masaki, Hyugo Ito, Yuki Oda, Kazufumi Yamazaki, Nobuhiro Tago, Kentaro Ohno, Nozomi Ishii, Hirosuke Tsunoda, Takashi Kanamori, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio
CHEMICAL COMMUNICATIONS
(2016)
Article
Chemistry, Multidisciplinary
Munefumi Tokugawa, Yoshiaki Masaki, Jan Christian Canggadibrata, Kazuhei Kaneko, Takashi Shiozawa, Takashi Kanamori, Morten Grotli, L. Marcus Wilhelmsson, Mitsuo Sekine, Kohji Seio
CHEMICAL COMMUNICATIONS
(2016)
Article
Chemistry, Physical
Hiroya Asami, Munefumi Tokugawa, Yoshiaki Masaki, Shun-ichi Ishiuchi, Eric Gloaguen, Kohji Seio, Hiroyuki Saigusa, Masaaki Fujii, Mitsuo Sekine, Michel Mons
JOURNAL OF PHYSICAL CHEMISTRY A
(2016)
Article
Chemistry, Organic
Takeshi Inde, Yoshiaki Masaki, Atsuya Maruyama, Yu Ito, Naoaki Makio, Yuya Miyatake, Takahito Tomori, Mitsuo Sekine, Kohji Seio
ORGANIC & BIOMOLECULAR CHEMISTRY
(2017)
Article
Chemistry, Organic
Takashi Kanamori, Akihiro Takamura, Nobuhiro Tago, Yoshiaki Masaki, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio
ORGANIC & BIOMOLECULAR CHEMISTRY
(2017)
Article
Chemistry, Organic
Kohji Seio, Munefumi Tokugawa, Kazuhei Kaneko, Takashi Shiozawa, Yoshiaki Masaki
Article
Biochemistry & Molecular Biology
Takeshi Inde, Shuhei Nishizawa, Yuusaku Hattori, Takashi Kanamori, Hideya Yuasa, Kohji Seio, Mitsuo Sekine, Akihiro Ohkubo
BIOORGANIC & MEDICINAL CHEMISTRY
(2018)
Article
Chemistry, Organic
Takahito Tomori, Kento Nagaoka, Leo Takeshita, Takashi Shiozawa, Yuya Miyatake, Yoshiaki Masaki, Mitsuo Sekine, Kohji Seio
JOURNAL OF ORGANIC CHEMISTRY
(2018)
Article
Chemistry, Organic
Kohji Seio, Takashi Kanamori, Yoshiaki Masaki
TETRAHEDRON LETTERS
(2018)
Article
Biochemistry & Molecular Biology
Yoshiaki Masaki, Yusuke Iriyama, Hiroyuki Nakajima, Yusuke Kuroda, Tatsuro Kanaki, Satoshi Furukawa, Mitsuo Sekine, Kohji Seio
NUCLEIC ACID THERAPEUTICS
(2018)
Article
Chemistry, Organic
Kohji Seio, Takashi Kanamori, Yoshiaki Masaki
JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY JAPAN
(2018)
Article
Biochemistry & Molecular Biology
Kentaro Ohno, Daiki Sugiyama, Leo Takeshita, Takashi Kanamori, Yoshiaki Masaki, Mitsuo Sekine, Kohji Seio
BIOORGANIC & MEDICINAL CHEMISTRY
(2017)